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Jurnal Analis Medika Biosains (JAMBS)
Published by Poltekkes Kemenkes Mataram
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
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Compliance with Iron Supplementation Consumption in Pregnant Women on Hematological Profiles and Ret-He Nugraha, Gilang; Edijanto, Subagijo Poegoeh; Masruroh, Nur
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.333

Abstract

Fetal growth will increase the mass of maternal and fetal erythrocytes resulting in an increase in iron requirements, causing a decrease in maternal iron stores.A decrease in iron in the body will disrupt the formation of hemoglobin, because one of the components that forms it is heme which contains iron. the aim of our research is to study the compliance of pregnant women in consuming iron supplements regarding hematological features and body iron reserves through the Ret-He examination. This research is a cross-sectional study in 30 pregnant women for complete blood count (CBC), Ret-He and estradiol. There were differences in the concentrations of Hgb and MCHC (P-value < 0.05) and there were no differences in the concentrations of Hct, RBC, MCV, MCH, RDW, Ret-He and estradiol (P-value > 0.05). It was found that there was a relationship between estradiol and Hgb (P-value = 0.047; r = 0.366). Pregnant women who do not comply with taking iron supplements experience a decrease in Hgb and MCHC. The decrease in Hgb levels is strongly correlated with E2 levels in pregnant women who adhere and do not comply with iron supplementation.
Pemisahan dan Penentuan Karakter Fraksi Protein ESA Toxoplasma gondii Secara ImmunologikUntuk Pengembangan Diagnosa Dewi, Lale Budi kusuma; Agrijanti, Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.216

Abstract

Toxoplasmosis is a zoonotic disease that attacks vertebrates including humans. T gondi infection cause toxoplasmic encephalitis in immunocompromised patients, blindness, abortion, fetal abnormalities and even fetal death or miscarriage. the diagnosis of toxoplasmosis is mainly based on detection of specific antibodies in serum samples using the T gondii antigen protein. various kinds of antigens are explored to be used as bioresptors to detect antibodies against T gondii in the patient's body. the use of ESA protein has advantages in terms of simple preparation. The lacks is  the protein contamination by extraparasitic components so that it can reduce the sensitivity and specificity of T gondii antibodies detection. The purpose of this study is to separate or purify antigenic ESA proteins to increase the sensitivity and specificity detection by purifying ESA proteins with gel filtration chromatography. The results obtained 3 fractionation, the protein ESA fractionation is one of fraction that has antigenic properties towards T Gondii. dipstick test with antigen using fraction 3 the results of ESA protein fractionation, 7 samples were positive from 8 positive samples, gave a sensitivity value of 87.5% and 6 samples were negative from 8 negative samples, giving a specificity value of 75%.
Indeks Formula Eritrosit Untuk Uji Skrining Talasemia Beta Minor Setiawan, Doni; Setiawan, Hendri; Nurmalasari, Ary
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.247

Abstract

Erythrocyte indices have been used as the first indicator in screening for beta-thalassemia minor. However, iron deficiency in hemoglobinopathies can reduce the sensitivity of screening, and the spectrum of mutations of different hemoglobinopathies between populations will result in discontinuation of the results of different erythrocyte indices. The research objective in this study was to determine the description of the erythrocyte indices used to screen for beta-thalassemia trait in Ciamis. The research method used in this study was a quantitative descriptive, using a cross-sectional study, data collection had been done during March-May 2021. The subjects of this study were 104 parents of a beta-thalassemia trait who have children with a beta-thalassemia major in Ciamis. The examination parameters used were complete blood count, with processing twenty-six erythrocyte formula index values. Ninety-two respondents were included in the inclusion criteria. The results of the erythrocyte indices in beta-thalassemia trait are England and Fraser, RBC, Mentzer, Srivastava, Shine and Lal, Ricerca, Green and King, Das Gupta, Jayabose, Telmissani-MCHD, Telmissani-MDH, Huber-Herklotz, Kerman I, Kerman II, Sirdah, Ehsani, Keikhaei, Nishad, Wongprachum, Sehgal, Pornprasert, Sirachainan, Bordbar, Matos and Carvalho and CRUISE has cut-off value in that range according to previous studies. Only the Bessman erythrocyte indices has a value of 17, exceeding the cut-off. Twenty-five erythrocyte indices with cut-off value according to previous studies can be used for beta-thalassemia screening in Ciamis, except for the Bessman erythrocyte indices.
Effect of Instant Coffee Consumption Duration on Low Density Lipoprotein (LDL) and Triglyceride Levels in the Community in Tanjung District, North Lombok Regency Suaryana, I Made Chandra; Wiadnya, Ida Bagus Rai; Dewi, Lale Budi Kusuma; Rohmi, Rohmi
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.475

Abstract

Long-term coffee consumption is thought to affect health, on blood fat levels such as Low Density Lipoprotein (LDL) cholesterol and Triglycerides. Knowing the effect of length of instant coffee consumption on Low Density Lipoprotein (LDL) and Triglyceride levels in the community in Tanjung District, North Lombok Regency. Analytic observation with a cross-sectional approach. Non-random sampling technique with a sample size of 30 respondents. Data analysis of one way anova test. Average Low Density Lipoprotein (LDL) levels in instant coffee consumers 1 year 175.5 mg/dl, 2 years 227.9 mg/dl, 3 years 296.2 and triglycerides 1 year 107.5 mg/dl, 2 years 165.8 mg/dl, 3 years 159.1 mg/dl. The results of the one-way anova test obtained a sig value of 0.006 ≤ 0.05 and triglycerides 0.007 ≤ 0.05.there is a significant effect of length of instant coffee consumption (1 year, 2 years, and 3 years) on Low Density Lipoprotein (LDL) and Triglyceride levels in the community in Tanjung District, North Lombok Regency. Where the longer the consumption of instant coffee, the higher the levels of Low Density Lipoprotein (LDL) and Triglycerides increased in the first year of instant coffee consumption, but decreased in the third year. This change may be influenced by body adaptation and other factors such as diet, physical activity, and health conditions.
Lymphocyte Cell Count Profile based on Widal Titer On Pediatric Typhoid Fever Nurmansyah, Dian; Fayumi, Syihab; Nisa, Shalehatun; Puspawati, Puspawati; Sasmitha, Maya; Mudzakkir, Musyirrah; Arsyad, Muhammad
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.480

Abstract

Typhoid fever remains a significant public health concern in Indonesia, which has the third-highest global case rate, with 350–810 cases per 100,000 people and a fatality rate of 3.1–10.4%, primarily due to delayed diagnosis and treatment. Children aged 5–14 years are most affected, with transmission occurring via contaminated food, water, or poor hygiene. Salmonella typhi invades intestinal immune tissues (Peyer’s patches), triggering responses from T and B lymphocytes, though bacterial endotoxins can suppress bone marrow function, altering leukocyte levels. This study analyzed the relationship between Widal titer and lymphocyte counts in pediatric typhoid patients using a cross-sectional design. Data from 526 patients at Graha Medika Martapura Clinic and Ratu Zalecha Hospital (January 2023–February 2025) were analyzed statistically, with inclusion criteria of Widal titer ≥1/320 and complete blood count results. Results showed 18.8% of patients had elevated lymphocytes, 79.3% had normal levels, and 1.9% exhibited lymphocytopenia. Higher Widal titers (1/640) correlated with lower lymphocyte counts (15.8% elevated vs. 23.0% at 1/320), suggesting immune suppression mediated by IL-6 through specific signaling pathways. Paradoxically, some cases showed lymphocytosis (18.8%), likely due to cytokine-induced immune modulation. Statistical analysis revealed a minimal correlation (R²=0.006), indicating Widal titer explains only 0.6% of lymphocyte variation. These findings demonstrate that while Widal titer has limited predictive value for lymphocyte changes, immune response mechanisms, particularly involving IL-6, play a critical role in typhoid fever's hematological manifestations.
Differences in Erythrocyte Indices in the First and Third Months of Hemodialysis in Chronic Kidney Failure Patients at Praya Regional Hospital Zahira, Jauhara Rana; Agrijanti, Agrijanti; Getas, I Wayan; Zaetun, Siti
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.464

Abstract

Hemodialysis therapy plays a role in replacing the kidneys' performance in filtering toxins and forming the hormone erythropoietin which forms and matures red blood cells. For this reason, it is necessary to conduct an examination of the erythrocyte index which functions to monitor the severity of anemia caused by chronic kidney failure. This study is a descriptive study with a cross-sectional approach. The sampling technique with purposive sampling is a technique with sample determination according to the researcher with a sample of patients with erythrocyte index examination data in the first and third months of undergoing hemodialysis. Data were analyzed using the Wilcoxon difference test. The total number of complete data of erythrocyte index examination in the first and third months was 22 patients. The average value of the first month erythrocyte index MCV 83.58 fL, MCH 28.31 pg, and MCHC 33.06 g/dL. In the third month the average value of the erythrocyte index MCV 85.35 fL, MCH 28.89 pg, and MCHC 33.90 g/dL. The results of the study stated a significance value of p 0.000 for MCV, MCH value p 0.015, and MCHC value p 0.005. There is a difference in the erythrocyte index in the first and third months of chronic kidney failure patients undergoing hemodialysis
Differences in Blood Glucose Levels Before and After the Consumption of Sweet Tea in People in Marong Village Aprilla, Annisa Suci; Diarti, Maruni Wiwin; Pauzi, Iswari; Jiwintarum, Yunan
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.454

Abstract

Blood glucose is the body's main energy source and is greatly influenced by the consumption patterns of food and beverages, especially those containing sugar. Uncontrolled increases in blood glucose levels can lead to metabolic disorders such as Diabetes Mellitus. Consumption of sweetened tea containing added sugar can cause a rapid spike in blood glucose levels, especially when consumed regularly and in excessive amounts. The purpose of this study is to find out the difference in blood glucose levels before and after sweet tea consumption in the Marong Village community. This study uses a pre-experimental design with a one-group pretest-posttest approach. A total of 16 respondents were selected using the purposive sampling technique. Blood glucose levels were checked before and two hours after the consumption of sweet tea using the POCT (Point of Care Testing) tool. Data were analyzed using a statistical paired sample t-test. The results of the study showed obtained the average blood glucose level before the consumption of sweet tea was 88.3 mg/dL, and after consumption was 97.3 mg/dL, with an average difference of 8.3 mg/dL. The results of the paired sample t-test showed a significance value of 0.000 (p < 0.05), which means that there is a statistically significant difference. This study concludes that the consumption of sweet tea has a significant influence on increasing blood glucose levels.  
The Effect Of Duration Of Tuak Consumption On Low Density Lipoprotein (LDL) And Triglyceride (TG) Levels In The Community In Taman Bali Village Manu, Thomas Tandi; Ananda, Dewa Ayu Putu Wiweka; Getas, I Wayan; Wiadnya, Ida Bagus Rai; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.544

Abstract

Tuak is a traditional alcoholic drink made from palm sap. Excessive alcohol consumption over a long period of time can affect lipid metabolism, increasing LDL and Triglyceride levels in the blood. Increased levels can cause cardiovascular disease. To determine the effect of the duration of tuak consumption on LDL and Triglyceride levels in the community in Dusun Taman Bali. The research used is Observational Analytical with a cross-sectional approach. The sample in this study was the community in Dusun Taman Bali who consumed Tuak for 1, 3, and 5 years with a total of 30 respondents using One Way Anova data analysis. The average LDL levels of 30 respondents with an average of 117.20 mg/dL consumed tuak for 1 year, 130.30 mg/dL for 3 years and 151.20 mg/dL for 5 years, while the average Triglyceride levels of 30 respondents who consumed Tuak for 1 year were 160.90 mg/dL, 166.60 mg/dL for 3 years and 201.10 mg/dL for 5 years. The results obtained showed a significant value of LDL levels of 0.000 while Triglyceride levels were 0.022. This means P <0.05, which means that there is an effect of the duration of tuak consumption of 1.3 and 5 years on LDL and Triglyceride levels in the community in Dusun Taman Bali.
Bacterial Resistance Test to Antibiotics Through Throat Swab Specimens of Children with Acute Respiratory Tract Infections (ARIs) Nurdin, Erpi; Zuchrullah, Mukhtasyam; Halik, Sofyanti Abdul
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.479

Abstract

Acute Respiratory Infections (ARIs) are contagious diseases that affect the respiratory tract and remain a leading cause of morbidity and mortality, particularly among children. The use of antibiotics without proper sensitivity testing can lead to bacterial resistance; therefore, research is needed to determine the effectiveness of commonly prescribed antibiotics. ARIs is one of the primary causes of morbidity and mortality, especially in children in developing countries. The irrational use of antibiotics or the absence of adequate sensitivity testing is one of the main factors contributing to the rise in bacterial resistance, which may reduce the effectiveness of treatment and increase the public health burden. Objective to determine the sensitivity levels of several types of antibiotics against bacteria that cause ARIs, obtained from throat swab specimens of children with ARIs in several community health centers (Puskesmas) under the Ternate City Health Office. This study is a descriptive research using a laboratory approach, applying the Kirby-Bauer method for antibiotic sensitivity testing against bacteria using Mueller Hinton Agar (MHA) media. Bacterial identification showed a predominance of Staphylococcus epidermidis (45%), followed by Streptococcus pneumoniae (35%), and Streptococcus pyogenes (20%). Sensitivity testing revealed that Streptococcus pneumoniae was most sensitive to gentamicin (100%), while Staphylococcus epidermidis showed high sensitivity to ciprofloxacin (100%). On the other hand, cefixime exhibited the highest level of resistance. Sensitivity testing is crucial before administering antibiotics to prevent resistance and ensure appropriate treatment for children with ARIs.
Examination of fingernail contents of Students at The Inpres Yoka Public Elementary School in Jayapura City, Papua Sinaga, Herlando; Rampa, Ester; Butiti, Lisa Chorani
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.536

Abstract

One environmental disease that is a public health concern is worm infection. Soil-transmitted helminths (STH) are a type of worm infection caused by various types of intestinal worms that spread through the soil. The purpose of this study was to determine the presence or absence of intestinal worm eggs in nail clippings from public elementary school students in Jayapura City. This study is descriptive in nature with a laboratory test approach. This study was conducted at the Microbiology Laboratory, Faculty of Health Sciences, Jayapura University of Science and Technology, from March 1, 2025, to June 1, 2025. The population in this study was all students attending public elementary schools in Jayapura City. The samples used in this study were nail clippings from elementary school students, which were examined using the flotation method on samples emulsified in a saturated NaCl solution, followed by microscopic identification. The results showed that of the 32 samples (100%) examined, 6 samples (18.75%) were positive for intestinal worm eggs, including 3 samples (9.37%) of Ascaris lumbricoides eggs (fertile) and Ascaris lumbricoides eggs (unfertile) in 3 samples (9.37%), and negative results without intestinal nematode eggs in 26 samples (81.25%).  

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