cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Syamsurizal
Contact Email
syam_unp@fmipa.unp.ac.id
Phone
-
Journal Mail Official
syam_unp@fmipa.unp.ac.id
Editorial Address
-
Location
Kota padang,
Sumatera barat
INDONESIA
Bioscience
Published by Universitas Negeri Padang
ISSN : 2614669X     EISSN : 2579308X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.
Arjuna Subject : -
Articles 146 Documents
 8   9   10   11   12 
Isolation and Identification of Probiotic Candidate Lactic Acid Bacteria (Lab) from Shrimp Paste (Mysis relicta) Based on 16s rRNA Gene Rahmat Wahyudi Putra; Resti Fevria
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151107569-0-00

Abstract

Terasi is one of the fermented product of shrimp or fish which is often used as food seasoning by the people. Terasi is also a source of Lactic Acid Bacteria (LAB) which has the potential as a culture for probiotic starters. Probiotics are dietary supplements containing live microbes that profitable to their hosts. This study aimed to identify probiotic lactic acid bacteria isolated from terasi of shrimp based on the 16S ribosomal ribonucleic acid (rRNA) gene sequence. LAB isolation from terasi was done by pour plate method using selective medium, MRSA (Man Rogosa Sharpe Agar). The identification of LAB in this study used a method of molecular with a marker gene of 16S rRNA. A total of 3 isolates were obtained from the isolation process. All LAB isolates are rod (Basil) positive Gram bacteria, negative catalase. Two isolates (TR 1 and TR 2) were identified as Enterococcus faecalis. While one more isolate (TR 3) were identified as Weissella cibaria.
Primer Construction to detect SNP rs11196205 Transcription Factor 7 Like 2 (TCF7L2) Using Amplification Refractory Mutation System (ARMS) PCR to detect Type-2 Diabetes Mellitus Elsa Badriyya; Afifatul Achyar
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042108497-0-00

Abstract

Type-2 Diabetes Mellitus (T2DM) is a metabolic disease characterized by an increase in blood glucose levels. The macrovascular and microvascular complications of T2DM can increase the risk of death in patient. SNP rs11196205 Transcription Factor 7 Like 2 (TCF7L2) gene has been associated with T2DM in several regions like Iceland, Denmark, the United State, and Thailand. SNP rs11196205 is characterized by polymorphism at position 113.047.288 from nucleotide Guanine to Cytosine. Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS PCR) is a method that can be used to detect mutation or polymorphism in a DNA. The aim of this research was to design a specific primer to detect SNP rs11196205 TCF7L2 gene using ARMS PCR. The design of study was a descriptive study to determine the specific primer. The method of the research includes DNA isolation, primer design using Geneious software, amplification of targeted area using ARMS PCR, and DNA sequencing method for bioinformatics study. Based on the research, four primers to detect SNP rs11196205 TCF7L2 gene have been successfully constructed. The primers were rs11196205-F, rs11196205-R, rs11196205-F(C) as a specific primer for C allele, and rs11196205- R(G) to detect G allele, the reaction produced 3 fragments of 856bp, 559bp, and 339bp.  
Anti-Microbial Activities of Shallots (Allium cepa L.) Extract and Garlic (Allium sativum L.) Extract on the Growth of Peat Soil Bacteria Chaidir Adam
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151110068-0-00

Abstract

This study aims to examine the antimicrobial activity of red onion (Allium cepa L.) and garlic (Allium sativum L.) extracts on the growth of peat soil bacteria. This type of research is an experimental study using a Completely Randomized Design with four levels of treatment. Test for bacterial activity using the agar diffusion method with the paper disc technique. Data were analyzed statistically using One Way Anova and the Games-Howell post-hoc test at the 5% significance level. The results showed that red onion (Allium cepa L.) and garlic (Allium sativum L.) extracts had antimicrobial activity against the growth of tested bacteria as indicated by the presence of a clear zone as an indicator of inhibition of bacterial growth. Garlic has better antimicrobial activity seen from the diameter of the clear zone that appears in the garlic extract treatment ranging from 8-13 mm, whereas in the treatment of onions it ranges from 3–5 mm. 
Morfometri Kepiting Air Tawar Parathelphulsa maindroni Dari Dua Tipe Habitat di Taman Nasional Gunung Palung Kalimantan Barat Junardi Junardi; Ina Idola; Tri Rima Setyawati
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042109331-0-00

Abstract

Freshwater crab Parathelphusa maindroni can live in various types of aquatic habitats. High adaptability can cause differences in body size in response to diverse habitat conditions. This species has been found at Stasiun Cabang Panti Gunung Palung National Park, West Kalimantan, in two different habitat types ununfortunately data on body size is still not available. The purpose of this study was to distinguish the morphometric characteristics of P. maindroni from two different habitats. The P. maindroni crabs were collected with traps and searched using a dip net. Morphometric analysis using 12 characters of body parts. The number of specimens was collected as many as 52 individuals, 28 individuals from river and 24 individuals from freshwater swamp habitat. The freshwater crab P. maindroni that lives in two different types of habitat can only be identified based on the width of the carapace, this character can also be used to differentiate of sex.
Identification of fungi in biogas mixed with buffalo dung and leaf onion waste (Allium cepa L.) Mei Niarti; Irdawati Irdawati; Moralita Chatri; Des M Des
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152106831-0-00

Abstract

Biogas is a gas produced from the decomposition process of organic materials by microorganisms in anaerobic conditions. Waste of red onion (Allium cepa L.) and buffalo dung contain organic substances and nutrients that can be used as biogas. The purpose of this study was to identify fungi in biogas mixed with buffalo dung with scallion waste (Allium cepa L.).The study was conducted from April to September 2019 in the Microbiology Laboratory and Integrated Research Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, State University. This research is a descriptive study by observing macroscopic and microscopic characters as a basis for identification produced by fungi in buffalo dung biogas (Pariaman, West Sumatra) and onion leaf waste from Alahan Panjang, West Sumatra.The results of this study obtained 5 genus of fungi on biogas substrate from a mixture of buffalo dung with scallion waste (Allium cepa L.) including: Aspergillus, Trichoderma, Candida, Mucor and Humicolla. Biogas adalah gas yang dihasilkan dari proses penguraian bahan-bahan organik oleh mikroorganisme dalam keadaan anaerob. Limbah daun bawang merah (Allium cepa L.) dan kotoran kerbau mengandung zat organik dan unsur hara yang dapat dijadikan sebagai biogas. Tujuan penelitian ini untuk mengidentifikasi jamur pada biogas campuran kotoran kerbau dengan limbah daun bawang merah (Allium cepa L.). Penelitian dilaksanakan dari bulan April sampai bulan September 2019 di Laboratorium Mikrobiologi dan Laboratorium Penelitian Terpadu Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri. Penelitian ini merupakan penelitian deskriptif dengan mengamati karakter makroskopis dan  mikroskopis  sebagai dasar identifikasi yang dihasilkan oleh jamur pada biogas kotoran kerbau (Pariaman, sumatera barat ) dan limbah daun bawang merah dari alahan panjang, sumatera barat. Hasil dari penelitian ini diperoleh 5 genus jamur  pada substrat biogas dari campuran kotoran kerbau dengan limbah daun bawang merah (Allium cepa L.) diantaranya yaitu: Aspergillus, Trichoderma, Candida, Mucor dan Humicolla.
Effect of SNEDDS (self-nanoemulsifying drug delivery system) kawista leaf aqueous extract (Limonia acidissima L.) on body and organ weight of Rats Giriani Ayu Sabilla; Slamet Widiyanto
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152113065-0-00

Abstract

SNEDDS (Self-Nanoemulsifying Drug Delivery System) is a pharmacological method that can increase the bioavailability of a drug. Kawista leaf aqueous extract contains secondary metabolites in the form of flavonoids, tannins, and saponins. SNEDDS preparation of kawista leaf aqueous extract has not been reported on its effect on body weight and organs of rats. The purpose of this study was to determine the effect of SNEDDS preparation of kawista leaf aqueous extract on body weight, liver and kidney weight of Rattus norvegicus in acute toxicity testing using the OECD 423 method. Data on body weight and organ weight of the liver and kidneys of female white rats of the Wistar strain were obtained during toxicity testing by acute OECD method 423. In this test, rats were divided into 3 treatment groups,namely control, SNEDDS, and SNEDDS of kawista leaf aqueous extract, with a total of 5 rats per treatment. The test was carried out in 2 terms of dosing according to OECD 423, wherein the second term the amount of the dose given was determined from the data on the response of the test animals to the dosage of the preparations in the first term. The results were analyzed using One Way ANOVA followed with Duncan. The results showed that the SNEDDS preparation of kawista leaf aqueous extract did not affect the body weight or the weight of the liver and kidneys of rats which was indicated from the statistical test results that there was no significant difference between treatments. This study conclude that SNEDDS preparation of kawista leaf aqueous extract did not affect the body weight or liver and kidney organs of rats.SNEDDS (Self-Nano Emulsifying Drug Delivery System) merupakan metode  yang dapat meningkatkan bioavailabilitas suatu obat. Ekstrak air daun kawista mengandung metabolit sekunder berupa flavonoid, tanin, dan saponin. Sediaan SNEDDS ekstrak air daun kawista belum dilaporkan pengaruhnya terhadap berat badan dan organ tubuh tikus. Tujuan penelitian ini adalah untuk mengetahui pengaruh sediaan SNEDDS ekstrak air daun kawista terhadap bobot badan, bobot hati dan bobot ginjal Rattus norvegicus pada uji toksisitas akut menggunakan metode OECD 423. Data bobot badan dan bobot organ hati dan ginjal tikus putih betina galur Wistar diperoleh selama pengujian toksisitas dengan metode OECD akut 423. Pada pengujian ini tikus dibagi menjadi 3 kelompok perlakuan yaitu kontrol, SNEDDS, dan SNEDDS ekstrak air daun kawista, dengan jumlah tikus per perlakuan sebanyak 5 ekor. Pengujian dilakukan dalam 2  dosis menurut OECD 423, dimana  jumlah dosis kedua yang diberikan ditentukan dari data respon hewan uji terhadap dosis pertama. Hasil dianalisis menggunakan One Way ANOVA dilanjutkan dengan Duncan. Hasil penelitian menunjukkan bahwa sediaan SNEDDS ekstrak air daun kawista tidak berpengaruh terhadap bobot badan atau bobot hati dan ginjal tikus yang ditunjukkan dari hasil uji statistik tidak terdapat perbedaan bermakna antar perlakuan. Penelitian ini menyimpulkan bahwa sediaan SNEDDS ekstrak air daun kawista tidak berpengaruh terhadap bobot badan, hati dan ginjal tikus.
Types of birds in oil palm plantation areas west pasaman district Hafizha Ihsan; Ramadhan Sumarmin
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152107162-0-00

Abstract

Birds that are members of vertebrate animals (vertebrates) that have feathers and wings, this taxonomic animal belongs to the Aves class. Birds have important roles in ecosystems, among others, as pollinators, seed dispersers, and pest control. Biodiversity in areas determined by habitat and level of human activity, as well as various species of birds in homogeneous forest areas such as oil palm. This research was conducted in January 2019 until May 2019, using the Visual Encounter Survey (VES) and Point Count method which was conducted in 3 observation sessions (morning, afternoon, evening). The research data will be presented in tabular form and analyzed descriptively. The results obtained are 3 species of birds in the area of oil palm plantations, namely Cekakak Shrub (Halcyon smyrnensis), Tekukur (Streptopelia chinensis), and Large Lathe (Centropus sinensis). Burung adalah anggota vertebrata yang memiliki bulu dan sayap, termasuk dalam Kelas Aves. Burung memiliki peran penting dalam ekosistem sebagai penyerbuk, penyebar benih, dan pengendalian hama. Keanekaragaman hayati di kawasan tersebut ditentukan oleh habitat dan tingkat aktivitas manusia, serta berbagai jenis burung di kawasan hutan homogen seperti kelapa sawit. Penelitian ini dilaksanakan pada bulan Januari sampai Mei 2019, dengan menggunakan metode Visual Encounter Survey (VES) dan Point Count dalam 3 waktu pengamatan (pagi, siang, dan sore). Total hanya 3 jenis burung yang ditemukan di daerah penelitian, yaitu Semak Cekakak (Halcyon smyrnensis), Tekukur (Streptopelia chinensis), dan Bubut Besar (Centropus sinensis).
Study of orchid resistance induced by Ceratorhiza sp. against ORSV infection based on peroxidase activity Anggi Anggreiny; Tundjung Tripeni Handayani; Mahfut Mahfut
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152112960-0-00

Abstract

Orchid is one of the popular ornamental plants that widely grown in Indonesia. However, the process of orchid cultivation is often hampered by virus infections. The virus that often infects the orchids is Odontoglossum ringspot virus. Viruses that enter orchid cells and replicate will activate the orchid’s defense response. This defense response is characterized by the increase of peroxidase activity. The peroxidase enzyme works in the formation of lignin to thicken cell walls and prevent viruses from entering other cells. In addition, the orchid defense response can also be activated through induced systemic resistance by inoculation of Ceratorhiza sp. as endophytic mycorrhizae. In this study, Factorial Complete Randomized Design (CRD) was used with 2 factors. Factor 1 is the type of orchid (Phalaenopsis amabilis and Dendrobium discolor) and factor 2 is the type of treatment (inoculation of mycorrhizae, virus, and mycorrhizae-virus). The orchid’s resistance level is determined by the analysis results of peroxidase activity using spectrophotometer. The results obtained indicate that all treatment combinations strongly influence the increase of peroxidase activity. Peroxidase activity of Phalaenopsis amabilis is 1.42 [(Umg) / min] and Dendrobium discolor is 1.64 [(Umg) / min] in average. Peroxidase activity on Dendrobium discolor was higher than on Phalaenopsis amabilis. This indicates that Dendrobium discolor has a higher level of resistance when compared to Phalaenopsis amabilis.Proses budidaya anggrek seringkali terkendala oleh infeksi virus. Adapun virus yang paling banyak menginfeksi anggrek adalah ORSV. Virus yang masuk ke dalam sel anggrek dan bereplikasi akan mengaktifkan respon pertahanan anggrek. Respon pertahanan ini ditandai dengan peningkatan aktivitas peroksidase. Respon pertahanan anggrek juga dapat diaktifkan melalui induksi resistensi sistemik dengan inokulasi Ceratorhiza sp. sebagai mikoriza endofit. Dalam penelitian ini digunakan Rancangan Acak Lengkap (RAL) Faktorial dengan 2 faktor. Faktor pertama adalah jenis anggrek (Phalaenopsis amabilis dan Dendrobium discolor) dan faktor kedua adalah jenis perlakuan (inokulasi mikoriza, virus, dan mikoriza virus). Tingkat ketahanan anggrek ditentukan dari hasil analisis aktivitas peroksidase menggunakan spektrofotometer. Hasil yang diperoleh menunjukkan bahwa semua kombinasi perlakuan sangat berpengaruh terhadap peningkatan aktivitas peroksidase. Aktivitas peroksidase Phalaenopsis amabilis rata-rata 1,42 [(U/mg)/ menit] dan Dendrobium discolor 1,64 [(U/mg)/ menit]. Aktivitas peroksidase pada Dendrobium discolor lebih tinggi dibandingkan dengan Phalaenopsis amabilis. Hal ini menunjukkan bahwa Dendrobium discolor memiliki tingkat ketahanan yang lebih tinggi jika dibandingkan dengan Phalaenopsis amabilis.
Microstructure of kase fish scale (Thryssa dussumieri Valenciennes, 1848) (Engraulidae) Fitri Nurhidayati; Abdul Razak
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152106584-0-00

Abstract

Kase fish (Thryssa dussumieri Valenciennes, 1848) is one of small pelagic fish from family engarulidae dan genus Thryssa. There is no information about microstructure of Kase fish scale yet. Generally, microstucture of fish scale consist of circulii, radii, focus, tubercle and lepidont. This study aims to identify scale  microstructure of  Kase fish. Sample of fish is taken in Padang beach waters. Fish scale was taken from bottom part of dorsal fin dan lateralis linear. Scale was cleaned by using KOH 5% during 10 menit. Scale was put into alkohol 30%, 50%, 70%, and 90% during 1 hour. After that, scale was dried before observed by usin Scanning Microscope Electron (SEM). Observation about scale microstructure of Kase fish centered to focus, anterior field and posterior field. Observation using 1000x , and 15000x zoom. Characteristic that is obsversed consist of shape of scale fish, focus, circulii, tubercle, anterior margin, ctenii and lepidont. The research result show that, based on it’s shape Kase fish scale is included to cycloid type with circular cordate subtype. In the anterior and posterior field are the circuli that compose intersiculus space. Circuli are bounded radii. In the circuli are also lepidont. The anterior and lateral margin of the scales are smooth. In the scales posterior field are tubercles and there is no stenii. The posterior margin are crenate.Ikan Kase (Thryssa dussumieri Valenciennes, 1848) merupakan salah satu ikan pelagis kecil dari famili engarulidae dan genus Thryssa. Belum ada informasi mengenai struktur mikro sisik ikan Kase. Secara umum struktur mikro sisik ikan terdiri dari sirkuli, jari-jari, fokus, tuberkulum dan lepidont. Penelitian ini bertujuan untuk mengidentifikasi struktur mikro skala ikan Kase. Sampel ikan diambil di perairan pantai Padang. Sisik ikan diambil dari bagian bawah sirip punggung dan lateral linier. Kerak dibersihkan dengan menggunakan KOH 5% selama 10 menit. Timbangan dimasukkan ke dalam alkohol 30%, 50%, 70%, dan 90% selama 1 jam. Kerak  dikeringkan sebelum diamati dengan menggunakan Scanning Microscope Electron (SEM). Pengamatan struktur mikro sisik ikan Kase berpusat pada fokus, bidang anterior dan bidang posterior. Pengamatan menggunakan 1000x, dan zoom 15000x. Ciri-ciri yang diamati terdiri dari bentuk sisik, fokus, sirkuli, tuberkel, tepi anterior, ctenii dan lepidont. Hasil penelitian menunjukkan bahwa berdasarkan bentuknya sisik ikan Kase termasuk tipe cycloid dengan subtipe cordate melingkar. Pada bidang anterior dan posterior terdapat sirkulus yang membentuk ruang intersiculus. Lingkaran adalah jari-jari yang dibatasi. Di sirkuli juga ada lepidont. Margin anterior dan lateral sisik halus. Pada bidang posterior sisik terdapat tuberkel dan tidak ada stenii. Margin posterior berbentuk crenate.
Isolation and characterization of lactic acid bacteria in fermented sweet corn (Zea mays l) as antibacterial Rosario Trijuliamos Manalu; Amelia Febriani; S. Syamsinar
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152113708-0-00

Abstract

Sweet corn (Zea mays L.) is a staple food that is known to produce Lactic Acid Bacteria (LAB) during the fermentation process, LAB is a probiotic bacterium that can produce antimicrobial components. This study aims to examine the characteristics and antibacterial activity of LAB sweet corn against Escherchia coli and Staphylococcus aureus.The study was conducted by isolating LAB from corn fermentation spontaneously which then tested the characteristics of the isolates obtained and tested for antibacterial activity. From the isolation results 3 isolates were obtained, namely FJM 1, FJM 2 and FJM 3 with morphological characteristics of rod-shaped and Gram-positive cells and biochemical test results; negative catalase, citrate, indole, motility, VP and positive TSIA. The results of antibacterial activity tests on E. coli were obtained respectively at 14.18 mm, 12.38 mm and 11.18 mm and S. aureus respectively at 13.05 mm, 11.12 mm and 10.35 mm. All three isolates had strong category antibacterial activity. Jagung manis (Zea mays L.) adalah makanan pokok yang diketahui menghasilkan Bakteri Asam Laktat (BAL) selama proses fermentasi, BAL merupakan suatu bakteri probiotik yang dapat menghasilkan komponen antimikroba. Penelitian ini bertujuan untuk menguji karakteristik dan aktivitas antibakteri BAL jagung manis terhadap Escherchia coli dan Staphylococcus aureus. Penelitian dilakukan dengan mengisolasi BAL dari fermentasi jagung secara spontan yang kemudian diuji karakteristik dari isolat yang didapatkan serta diuji aktivitas antibakterinya. Dari hasil isolasi didapatkan 3 isolat yaitu FJM 1, FJM 2 dan FJM 3 dengan karakteristik morfologi sel berbentuk batang dan Gram positif serta hasil uji biokimia; katalase, sitrat, indol, motilitas, Vp negatif dan TSIA positif. Hasil uji aktivitas antibakteri terhadap E. coli didapatkan berturut sebesar 14,18 mm, 12,38 mm dan 11,18 mm dan S. aureus berturut sebesar 13,05 mm, 11,12 mm dan 10,35 mm. Ketiga isolat memiliki aktivitas antibakteri kategori kuat.

Page 10 of 15 | Total Record : 146

 8   9   10   11   12 

Filter by Year

2017 2024


Reset
Filter By Issues
All Issue Vol 8, No 2 (2024): Biology Vol 8, No 1 (2024): Biology Vol 7, No 2 (2023): Biology Vol 7, No 1 (2023): Biology Vol 6, No 2 (2022): Biology Vol 6, No 1 (2022): Biology Vol 5, No 2 (2021): Biology Vol 5, No 1 (2021): Biology Vol 4, No 2 (2020): Biology Vol 4, No 1 (2020): Biology Vol 3, No 2 (2019): Biology Vol 3, No 1 (2019): Biology Vol 2, No 2 (2018): Biology Vol 2, No 1 (2018): Biology Vol 1, No 2 (2017): Biology Vol 1, No 1 (2017): Biology More Issue