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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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ANALYSIS OF DENGUE SPECIFIC IMMUNE RESPONSE BASED ON SEROTYPE, TYPE AND SEVERITY OF DENGUE INFECTION Ade Rochaeni; Aryati Aryati; Puspa Wardhani; Usman Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1199

Abstract

Infeksi Virus Dengue (IVD) menimbulkan derajat klinis beragam dari DD hingga DBD/SSD. Respons imun spesifik dengue berupaIgM dan IgG anti dengue masih merupakan perdebatan untuk patogenesis DBD di samping faktor virulensi virus dan jenis infeksi.Penelitian ini bertujuan untuk menganalisis respons imun spesifik dengue terhadap serotipe, jenis dan derajat IVD di Surabaya. Subjekadalah pasien IVD yang dirawat di Ruang Tropik Infeksi Penyakit Dalam RSUD Dr. Soetomo dengan hasil penyaringan uji cepat NS1 (SDBioline Dengue Duo) dan/atau PCR (Simplexa Dengue) positif. Pemeriksaan IgM dan IgG anti dengue kuantitatif dengan metode ELISA(Panbio Dengue Duo IgM and IgG Capture). Penelitian dilakukan Maret–Agustus 2016 dan didapatkan 61 pasien dengan hasil NSI dan/atau PCR dengue positif. Identifikasi serotipe didominasi DEN-3, namun serotipe yang lebih virulen ditunjukkan DEN-1 yaitu semuapasien bermanifestasi sebagai infeksi sekunder dan DBD. Jenis infeksi primer sebanyak 19 (31,1%) dan infeksi sekunder 42 (68,9%).Derajat IVD meliputi DD 10 (16,4%), DBD 47 (77%) dan SSD 4 (6,56%). Nilai indeks rerata IgM dan IgG anti dengue di kelompokinfeksi serotipe DEN-1 (5,140 dan 5,774), DEN-2 (2,971 dan 2,222), DEN-3 (1,863 dan 2,792); kelompok jenis infeksi primer (1,478 dan0,746), sekunder (4,028 dan 4,864) dan kelompok derajat DD (1,170 dan 1,492), DBD I (3,370 dan 3,651), DBD II (3,924 dan 4,439)dan DBD III (4,164 dan 4,243). Sebagai simpulan respons imun spesifik dengue didapatkan lebih tinggi bermakna di kelompok infeksiserotipe DEN-1, kelompok jenis infeksi sekunder dan kelompok DBD/SSD.
EVALUATION OF BLOOD GLUCOSE TESTING USING CONTOUR® PLUS GLUCOMETER Beauty, Venny; Sukartini, Ninik
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1340

Abstract

The use of glucometers has been widely recommended to help patients in controlling their blood glucose level. This study compared the blood glucose level measured by Contour® Plus glucometer and Cobas c501 chemistry analyzer, as the reference method. The study design was cross-sectional and conducted in the Dr. Cipto Mangunkusumo Hospital in April 2017. Study materials were 120 capillary blood examined by Contour® Plus glucometer and plasma analyzed by Cobas c501 chemistry analyzer. Precision, correlation, accuracy, and clinical accuracy tests were performed based on ISO 15197:2013, using Parkes error grid analysis. Contour® Plus glucometer yielded a CV of 1.56-2.2%, following the recommendation of the American Diabetes Association, of <5%, there was a strong positive correlation between the glucose level of capillary blood and plasma (r=0.997). Accuracy test based on ISO 15197:2013 showed that 100% of capillary blood glucose deviations were within the ±15 mg/dL range for glucose level <100 mg/dL and ±15% range for glucose level ≥100 mg/dL. Clinical accuracy test with Parkes error grid showed 100% of results were in zone A. Contour® Plus glucometer test results met the ISO 15197:2013 criteria, so the results were proportional to the reference method’s results and clinically acceptable. Contour® Plus glucometer is safe to be used in blood glucose monitoring, as long as careful attention is given to the device specifications.
ERROR RATE OF DISC DIFFUSION METHOD IN CEFTAZIDIME/ CEFOTAXIME SUSCEPTIBILITY TEST ON CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE (Laju Kesalahan Uji Kepekaan Ceftazidim/Cefotaxime Metode Difusi Cakram pada Klebsiella pneumoniae) Luz Maria GBW; Osman Sianipar; Usi Sukorini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1233

Abstract

Klebsiella pneumoniae merupakan salah satu bakteri Gram negatif yang banyak menimbulkan infeksi nosokomial. Difusi cakrammerupakan satu metode uji kepekaan antimikrobia yang banyak digunakan di laboratorium klinik yang juga dapat dipakai untuk menyaringK.pneumoniae penghasil enzim Extended-Spectrum β-Lactamase (ESBL). Antimikrobia pilihan untuk infeksi ini adalah cephalosporin generasiketiga (ceftazidime dan ceftotaxime). Penelitian ini bertujuan untuk mengetahui laju kesalahan uji kepekaan ceftazidime/cefotaxime metodedifusi cakram di isolat klinis K.peumoniae. Penelitian ini merupakan kajian potong lintang yang melibatkan 53 isolat klinis K.pneumoniae.Uji kepekaan ceftazidime/cefotaxime di isolat klinis K.pneumonia dilakukan menggunakan metode difusi cakram dan uji E sebagai rujukan.Hasil memeriksa dilaporkan dalam bentuk kepekaan, intermediate dan resistensi untuk setiap obat, dianalis untuk mengetahui laju kesalahan(minor error, major error dan very major error). Isolat klinis terbanyak berasal dari darah, air kemih dan nanah, berturut-turut 32,1%, 32,1%dan 18,9%. Sebagian besar isolat didapat dari ruang perawatan non-intensif (86,8%). Minor error uji kepekaan ceftazidime/cefotaximemetode difusi cakram berturut-turut 7,55% dan 1,89%, sehingga dapat disimpulkan bahwa metode difusi cakram uji kepekaan ceftazidime/cefotaxime dapat digunakan dalam uji kepekaan terhadap isolat klinik K.pneumoniae.
THE DIFFERENCES OF SODIUM, POTASSIUM AND CHLORIDE LEVELS IN STEMI AND NSTEMI PATIENTS Freddy Ciptono; Muji Rahayu
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1163

Abstract

Infark miokard telah menjadi salah satu penyebab terbesar kematian di seluruh dunia yaitu sebesar 30% dari angka kematiansecara global. STEMI dan NSTEMI dibedakan berdasarkan derajad iskemia yang dialami berupa elevasi segmen ST di pemeriksaan EKGdi pasien STEMI. Peningkatan kadar enzim jantung juga ditemukan baik pada STEMI maupun NSTEMI. Beberapa penelitian terdahulumengungkapkan adanya perubahan kadar elektrolit di pasien infark miokard. Penelitian ini bertujuan untuk menganalisis perbedaankadar natrium, kalium dan klorida di pasien STEMI dan NSTEMI. Penelitian potong lintang, subjek pasien STEMI dan NSTEMI yangdirawat di RSUP Dr. Kariadi, Semarang keluhan angina yang khas, pemeriksaan EKG dengan atau tanpa elevasi segmen ST, disertaidengan peningkatan kadar enzim jantung melebihi batas diagnostik. Data natrium dan klorida dianalisa menggunakan student’s t-testdan data kalium dianalisa menggunakan Mann Whitney U Test. Perbedaan bermakna apabila p< 0,05. Penelitian ini dilakukan di 60subjek dengan rerata umur 53,9 tahun pada kelompok STEMI dan 58,47 pada kelompok NSTEMI. Terdapat perbedaan bermakna padakadar natrium antara pasien STEMI dan NSTEMI (p=0,00). Kadar kalium (p=0,625) dan klorida (p=0,423) tidak memiliki perbedaanbermakna antara dua kelompok tersebut. Terdapat perbedaan yang bermakna antara kadar natrium di pasien STEMI dengan NSTEMI(p< 0,05). Kadar natrium yang didapatkan lebih rendah di pasien STEMI dapat menjadi salah satu petanda laboratorium dalammelakukan diagnosis banding antara STEMI dengan NSTEMI.
MUTU LAYANAN MENURUT PELANGGAN LABORATORIUM KLINIK (Service Quality Regarding to The Clinical Laboratory Customer) Mohammad Rizki; Osman Sianipar
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1287

Abstract

Customer satisfaction survey for outpatient laboratory customer is routinely carried out in Clinical Laboratory InstallationRSUP Dr. Sardjito using modified SERVQUAL questionnaire. One advantage of using SERVQUAL is service provider will be able to monitorits service quality dynamics using standardized tool. Changes in one’s service quality can be measured using perceived quality changesbased on gap scorebetween different periods. All these years, RSUP Dr. Sardjito has not evaluated gap score changes in all survey period.This study aims to know service quality measured by perceived service quality on Customer Satisfaction Survey Period of Semester II2013 and Semester I 2014 by comparison. This is an observational non-experimental study using data from Customer SatisfactionSurvey in Clinical Laboratory Installation RSUP Dr. Sardjito Semester II 2013 and Semester I 2014. Data was analysed using SERVQUALmethod and presented descriptively as text and table. There were 231 and 229 responders respectively in Customer Satisfaction SurveySemester II 2013 and Semester I 2014. There were decreases in all but empathy service dimension. Decrease of expectation was foundin all SERVQUAL dimensions. The decrease of expectation exceeded perception decrease resulting in a rise of gap score from Semester II2013 to Semester I 2014 hence indicating an improvement of perceived service quality. There was an improvement of perceived servicequality according to external customer of Clinical Laboratory Installation RSUP Dr. Sardjito in Semester I 2014 compared to SemesterII 2013.
CORRELATION BETWEEN NT-PROBNP AND LEFT VENTRICULAR EJECTION FRACTION BY ECHOCARDIOGRAPHY IN HEART FAILURE PATIENTS Mutiara DS; Leonita Anniwati; M. Aminuddin
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1131

Abstract

Petanda biologis NH2-terminal fragment of proBrain Natriuretic Peptide (NT-proBNP) berguna untuk diagnosis dini, menyingkirkangejala klinis yang berasal dari luar jantung serta pemantauan pengobatan dan meramalkan perjalanan penyakit pasien gagal jantung.Pemeriksaan NT-proBNP dapat dilakukan secara otomatis, sehingga hasil tidak bersifat subjektif. Pemeriksaan ekokardiografi merupakanpemeriksaan penunjang yang telah umum digunakan untuk mendiagnosis gagal jantung. Namun, pemeriksaan ekokardiografi tidakselalu tersedia di seluruh rumah sakit, khususnya rumah sakit di daerah, serta memerlukan tenaga ahli untuk melakukan pemeriksaandan hasil pemeriksaan bersifat subjektif. Salah satu tolok ukur yang dinilai pada pemeriksaan ekokardiografi adalah fraksi ejeksiventrikel kiri. Penelitian ini bertujuan untuk mengetahui kenasaban antara kadar NT-proBNP dengan fraksi ejeksi ventrikel kiri yangdiperoleh dari pemeriksaan ekokardiografi. Penelitian bersifat quasi experimental dengan pendekatan pretest and posttest only withoutcontrol. Sampel penelitian berjumlah 41 orang, dikumpulkan selama bulan Februari–April 2015 dari Ruang Perawatan Jantung RSUDDr. Soetomo Surabaya. Pemeriksaan kadar NT-proBNP menggunakan metode chemiluminescent (Immulite 1000) dengan prinsip solidphasetwo site chemiluminescent immunometric assay. Hasil dianalisis secara statistik menggunakan uji kenasaban Spearman’s, ujit 2 sampel berpasangan, Kruskal Wallis dan Mann Whitney. Rentang kadar NT-proBNP sebelum dan sesudah pemberian pengobatandi pasien gagal jantung masing-masing antara 1.296–34.374 pg/mL dengan rerata 10.422,49 pg/mL (Simpang Baku (SB) 8.608,05)dan 997–34.401 pg/mL dengan rerata 8.899,41 pg/mL (SB 8.489,46). Rentang persentase fraksi ejeksi ventrikel kiri sebelum dansesudah pemberian pengobatan di pasien gagal jantung masing-masing antara 20–62% dengan rerata 35,61% (SB 10,00) dan 22–71%dengan rerata 41,49% (SB 10,96). Didapatkan perbedaan bermakna rerata kadar NT-proBNP serta persentase fraksi ejeksi ventrikel kirisebelum dan sesudah pemberian pengobatan di pasien gagal jantung dengan setiap nilai p=0,001. Didapatkan kenasaban negatif yangbermakna antara kadar NT-proBNP dan fraksi ejeksi ventrikel kiri di pasien gagal jantung sebelum dan sesudah pemberian pengobatandengan masing-masing nilai p=0,001, r=-0,81 dan nilai p=0,001, r=-0,80. Didapatkan kenasaban negatif yang bermakna antarakadar NT-proBNP dengan fraksi ejeksi ventrikel kiri di pasien gagal jantung sebelum dan sesudah pemberian pengobatan. Berdasarkanhal tersebut maka pemeriksaan petanda biologis NT-proBNP dapat diusulkan untuk digunakan sebagai tolok ukur pilihan penggantiekokardiografi untuk gagal jantung.
HASIL HITUNG NORMOBLAS ANTARA SEDIAAN HAPUSAN DARAH TEPI PENDERITA AML DENGAN ALL Hidayat .; Nina Susana Dewi; Nadjwa Zamalek Dalimoenthe
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.997

Abstract

Normoblast is an immature form of erythrocyte in erythropoietin system. Normally, normoblast can be found in peripheral blood healthy neonates. The existence of normoblast in peripheral blood might be the sign of pathologic conditions such as hemolytic anemia,acute blood loss, and ischemia and bone marrows abnormalities like malignancy or leukemia. In acute leukemia (Acute MyeloblasticLeukemia and Acute Lymphoblastic Leukemia), normoblast existence in peripheral blood may due to erythropoietin system suppression.The aim of this study is to compare normoblast count between AML and ALL, and also to find out the correlation between leukocyte andnormoblast count in AML and ALL. The subject of this study were patient diagnosed as AML (30) and ALL (30) in Hematology Divisionof Clinical Pathology Department at Dr.Hasan Sadikin Hospital Bandung in July 2006–August 2008. In this study we examined 30peripheral blood smears from AML and 30 peripheral blood smears from ALL. Leukocyte count result was derived from CBC performedwith Sysmex KX-21. The mean value of normoblast count from AML blood smear patients is 1930.60 (3.60/100 WBC) while ALL bloodsmear patients is 309.60 (0.43/100 WBC). Statistically this difference is significant (p < 0.001). There are strong correlation betweenleukocyte count and normoblast count within both group (r = 0.851, r = 0.948; p < 0.001).
ALBUMIN KREATININ PENDERITA HIPERTENSI HAKIKI (ESENSIAL) T. Wongso; Dewi LS; Z. Lubis
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 2 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i2.904

Abstract

Increasing of urine albumin excretion in hypertension patient is early sign of renal excretion disorder. Increasing of urine albuminexcretion could not detect with conventional methods, but with more sensitive methods, one of them is examined ratio of urine albuminand urine creatinine (ACR). Knowing ACR rate in essential hypertension. This research was done by cross sectional with consecutivesampling of hypertension patient with JNC VII 2003 criteria. Research used quantitative urine albumin with Albumin Tina QuantMethods draught Immunoturbidinetry. The urine creatinine was analyzed with Jaffe Methods using Roche Hitachi 902. From 25 peopleof hypertension group found ACR < 30 mg/g amount 16 people and ACR rate 30–300 mg/g amount 9 people. Mean while from 22people of non hypertension, all had ACR < 30 mg/g. In this research also found strong correlation between ACR with hypertension,diastolic and systolic blood pressure. It was found that ACR rate of hypertension group was higher than the non hypertension group. Itwas also found a strong relation between ACR with hypertension, systolic and blood diastolic pressure.
DIFFERENCE IN GLICATED HEMOGLOBIN LEVEL BETWEEN BORONATE AFFINITY METHOD AND ION EXCHANGE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD IN TYPE 2 DIABETES MELLITUS Tuti Asryani; Ellyza Nasrul; Rikarni Rikarni; Tutty Prihandani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1466

Abstract

Background: Glycated Hb (HbA1c) test is needed to control glycemic in high prevalence type 2 diabetes mellitus (DM) patients. Hemoglobin fraction separated and chemical reaction are two main concepts in HbA1c test. Ion exchange-high performance liquid chromatography (HPLC) and boronate affinity use first concept. Ion exchange-HPLC is reference method in most of clinical laboratorium. Point of care testing (POCT) with boronate affinity method that has been standardized by international institution is available. The aim of this study was to compare boronate affinity POCT method and ion exchange-HPLC method.Method: This cross sectional study was conducted to 22 type 2 DM patients those fullfill inclusion and exclusion criteria in January 2017 to February 2018. Level of HbA1c was assayed with boronate affinity POCT and ion exchange-HPLC method. T-test was used to analyse data and no significance difference if p>0.005Results: Subjects of this study are women (59.1%) more than men (40.9%) with age mean 59.23 years old (8.1). Uncontroled type 2 DM (77.3%) more than controled type 2 DM (22.7%).Mean of HbA1 level was 8.0% (1.7) in boronate affinity POCT and 8.3% (1.8) in ion exchange-HPLC. T-test showed no significance difference between those two HbA1C assay methods (p>0.005).Conclusion: There was no difference HbA1c level between boronate affinity POCT method and ion exchange-HPLC method.
MASALAH KETAHANAN (RESISTENSI) VANCOMYCIN TERHADAP ENTEROCOCCI urhayana Sennang AN
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 1 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i1.957

Abstract

Enterococcus adalah bakteri komensal dalam usus (colon) manusia dan binatang, dapat menyebabkan infeksi saluran kemih,endokarditis dan infeksi intra abdominal. Vancomycin-resistant enterococci (VRE) merupakan masalah kesehatan utama di berbagainegara. VRE yang merupakan cadangan (reservoir) glycopeptide resistance dianggap dapat menjangkit ke manusia melalui persentuhan(kontak) dengan binatang atau memakan (konsumsi) daging. Walaupun E. faecalis lebih sering terjadi infeksi di manusia, tetapivancomycin resistance lebih sering ditemui di isolat E. faecium. VRE merupakan patogen pada populasi imunokompromis terutamapenderita yang mendapatkan berbagai antibiotik dan menjalani rawat inap yang lama. terdapat enam tipe glycopeptide resistanceyang dilaporkan mengenai enterococcus yaitu VanA, VanB, VanC, VanD, VanE dan Vang. VRE merupakan salah satu penyebab infeksinosokomial dan kerentanannya (kemampuan resistensinya) dapat berpindah antar organisme atau spesies lainnya. Untuk itu kebijakanpengendalian infeksi (infection control) dan panduan pemberian antibiotik sangat penting diterapkan untuk mengendalikan penyebaranVRE dan organism yang rentan (resisten) terhadap berbagai obat.

Page 13 of 133 | Total Record : 1328

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