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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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ANALYSIS OF LABORATORY PARAMETERS AS SEPSIS MARKERS IN NEONATALS WITH HYPERBILIRUBINEMIA Bachtiar Syamsir; Rachmawati Muhiddin; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1154

Abstract

Hiperbilirubin di neonatus merupakan kondisi peningkatan bilirubin total (> 5mg/dL) di bayi baru lahir. Angka kejadian di bayicukup bulan mencapai 60%. Kejadian hiperbilirubin di bayi baru lahir salah satu penyebab terbanyak bayi harus mendapat perawatan.Kejadian hiperbilirubinemia di neonatus yang disebabkan oleh sepsis 3–8%. Tujuan penelitian ini untuk mengetahui hubunganpeningkatan petanda sepsis C-Reaktif Protein (CRP), Procalcitonin (PCT), rasio jumlah imature to total neutrofil (IT ratio) dan jumlahtrombosit di neonatus dengan hiperbilirubinemia dan menilai kenasabannya. Penelitian retrospektif dengan pendekatan potong lintangdengan mengambil data rekam medis pasien yang diperiksa kadar bilirubin total di RSUP Dr. Wahidin Sudirohusodo Makassar masawaktu Januari-Desember 2015. Patokan kesertaan, pasien dengan pemeriksaan petanda sepsis CRP, PCT, IT Ratio dan trombosit,dengan diagnosis akhir sepsis. Data dikelompokkan menjadi kelompok hiperbilirubin dan non-hiperbilirubin, diuji dengan Uji MannWhitnney atau Uji T Independen untuk melihat hubungan antara keempat petanda sepsis dengan kelompok bilirubin. Data kelompokhiperbilirubinemia kemudian diuji kenasaban Spearman untuk melihat kenasaban keempat petanda sepsis dengan kadar bilirubin.Diperoleh total 92 sampel yang memenuhi patokan kesertaan, kelompok hiperbilirunemia 67,4% dan non-hiperbilirubinemia 32,6%.Hasil penelitian menunjukkan tidak terdapat perbedaan bermakna nilai keempat petanda sepsis diantara kedua kelompok (p>0,05),terdapat kenasaban negatif jumlah trombosit dengan sepsis hiperbilirubinemia (p<0,05; r.-0,261). Tidak dapat disimpulkan adanyahubungan CRP, PCT, IT ratio dan trombosit terhadap kejadian hiperbilirubinemia. Pada subjek dengan hiperbilirubinemia, makin tinggikadar bilirubin makin rendah jumlah trombosit.
KADAR SURFACTANT PROTEIN-D SERUM PADA PASIEN PENYAKIT PARU OBSTRUKTIF KRONIS BERKEBAHAYAAN KAMBUHAN RENDAH DAN TINGGI Dewi Nurhayati; Ida Parwati; Tiene Rostini; Arto Yuwono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1122

Abstract

Chronic obstructive pulmonary disease (COPD) cause high morbidity and mortality worldwide. The exacerbations in chronicobstructive pulmonary disease accelerate the decline in lung function and health status, as well as in increasing the treatment cost andmortality risk. The spiro metric measurement has several limitations in assessing the severity as well as the exacerbation risk in COPDpatients. Currently, has been available serum surfactant protein-D, a marker of lung inflammation and lung tissue damage. This proteinis produced by the alveolar type II cells and the Clara cells that play role in maintaining the lung stability and pulmonary immunesystem. The increased level of serum SPD indicates that there is lung epitihelial leakage in line with COPD severity increment and reflectedin COPD exacerbation level of risk according to combined COPD assessment GOLD criteria of the year 2011. The aim of this study wasto know the differences of SP-D serum levels between low and high risk of exacerbation in COPD patients by determination them. Thisstudy was conducted from March to July 2014. The subjects of this study were COPD patients diagnosed by spiro metric measurement.The research was conducted in comparative analytic way with a cross sectional study design. The statistical analysis was performedusing Mann-Whitney non-parametric test. The subjects were 62 COPD patients. The SP-D serum level at low risk of exacerbation groupdiffer significantly compared to the high risk exacerbation group, 1.8–68.4 ng/mL and 3.36–116.4 ng/mL respectively (P=0.018).Based on this study it can be concluded that the SP-D serum levels were higher in COPD patients with high risk exacerbation than thelower risk one. The SP-D serum levels may be considered as a specific marker of lung tissue injury to assess the risk of the exacerbationin COPD patients.
PATOGENESIS dan PEMERIKSAAN LABORATOPRIUM MIELOFIBROSIS PRIMER Johanis Johanis; Arifoel Hajat
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1025

Abstract

Primary myelofibrosis (PMF) is a neoplastic hematologic disease, characterized by clonal hemapoietic stem cell and collagenaccumulation in bone marrow. PMF is not related with underlying myeloproliferative disorders or other diseases. The features of PMFshow marrow fibrosis, megakaryocytes and granulocytes proliferation, and extramedullary hemapoiesis. PMF is classified as BCR-ABLnegativemyeloproliferative disorders. Diagnosis of PMF is based on clinical symptoms of splenomegaly and myelopthisis; bone marrowbiopsy shows granulocytes/megakaryocytes hyperplasia, megakaryocytes dysmorphic and fibrosis; cytogenic testing for mutation ofJAK2V617F+, MPLW5I5L/K+ and BCR-ABL. The diagnostic criterion according to the 2008 WHO classification considers major andminor crieria. The primary purpose for treatment is to improve the quality of life by paliative tratment. The five year survival of PMFpatients is low.
PROFIL ASAM LAKTAT PENDERITA DIABETES MELLITUS TERKENDALI (KONTROL) DAN TIDAK TERKENDALI (KONTROL) Laily Indrayanti; Harjo Mulyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 3 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i3.927

Abstract

Lactic acid is an intermediate product of carbohydrate metabolism. Increment of plasma lactic acid level usually correlated withaerobic metabolism defect which caused by hypoperfusion or hypoxia, that can be happened in DM (Diabetes Mellitus) patients. DiabetesMellitus is a risk factor of lactic acidosis. Determination of glycated Hb (HbA1c) is a parameter to monitor the blood glucose. The aimof this study is to compare the mean lactic acid level between uncontrolled and controlled DM) patients and their correlation betweenlactic acid and HbA1c level. The research carried out by cross sectional study which was done at the Clinical Pathology Laboratory ofSardjito Hospital between September–October 2007. Inclusion criteria of samples were diabetic patients who had HbA1c examination.Statistical analysis was done by independent t test and Pearson correlation test. Twenty one patients were included in this research.They were divided into two (2) groups, group I are those who had HbA1c ≤ 7%, they consist of 10 patients, group II are patients whohad HbA1c ≥ 7.1%, they consist 11 patients. The mean of lactic acid of group I was 1.85 mmol/L and group II was 1.74 mmol/L (p = 0.574). There wasn’t any significant correlation between HbA1c level and lactic acid. (r = -0.179, p = 0.437). The mean of lacticacid level in uncontrolled DM was lower than the controlled one but not significant, and there was no significant correlation betweenHbA1c level and lactic acid. It is suggested to continue this study but with larger sample to know the correlation between lactic acid andHbA1c in DM patients who had metformin therapy.
SD DENGUE DUO® (NS1, IgG, IgM) RAPID TEST DALAM MENUNJANG DIAGNOSIS INFEKSI VIRUS DENGUE Diah Puspita Rini; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.980

Abstract

Dengue virus infection can cause dengue hemorrhagic fever (DHF) which is still a major health problem in Indonesia. Thediagnosis of DHF is established based on WHO criteria and IgM/IgG antidengue serologic markers. A rapid method is needed for thedetecting or screening the disease. Antigen detection (NS1) can be performed by immunochromatography (rapid test) or enzyme -linkedimmunosorbent assay (ELISA). Recently, a rapid test to detect NS1 as an antigen and IgM/IgG antidengue to differentiate primary andsecondary dengue virus infection is available in one cassette. This study evaluated the new commercial dengue rapid test, SD DengueDuo for the detection of both antigen and antibodies to dengue virus. Serum samples used in this study were collected from 33 denguevirus infection patients according to WHO criteria and admitted in the Tropical Ward, Dr. Soetomo Hospital. Samples were taken twice,during acute and convalescent phase. SD Dengue Duo (NS1, IgG, IgM) rapid test was used and confirmed by ELISA as the gold standard.To determine the diagnostic specificity 20 samples of non dengue virus infection (typhoid fever and malaria) confirmed by laboratorytests were used. In the acute phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed IgG sensitivity 94.7% (18/19), specificity 92.9%(13/14), IgM sensitivity 85% (17/20), specificity 100% (13/13), NS1 sensitivity 50% (10/20), specificity 100% (13/13). In theconvalescent phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed antidengue IgG sensitivity 96.3% (26/27), specificity 66.7%(4/6), IgM sensitivity 95% (22/23), specificity 80% (8/10), NS1 sensitivity 42.9% (3/7), specificity 100% (26/26). To establish thediagnosis of dengue virus infection, not only NS1, but also antidengue IgM/IgG is needed. SD Dengue Duo containing dengue NS1 antigencombined with IgG/IgM test in one cassette is a rapid, practical and has a high validity diagnostic result.
PROFIL LIPID PENDERITA DIABETES MELLITUS TIPE 2 P S Josten; Mutmainnah Mutmainnah; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 1 (2006)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i1.894

Abstract

Prevalence of type 2 diabetes mellitus (DM) tends to increasing worldwide. The main cause of death in type 2 DM is coronaryheart disease (CHD) and its mortality rate can increase 2 to 4 times compared to non-diabetics. One of the risk factors in CHD isdyslipidemia. To know the lipid profile based on age and gender and to assess the relation of total cholesterol, LDL, HDL, and TG levelto age. Descriptive retrospective study in patients with type 2 DM who are 45 years old and over. From 100 Type 2 DM patients, in theDepartment of Internal Medicine, Dr. Wahidin Sudirohusodo Hospital, Makassar, period of June to December 2005, the largest age groupwith dyslipidemia was > 59 years old, with increase LDL level, 32.73% in males and 46.67% in females. There was a significant relationbetween the in crease of TG (p = 0.03) and the decrease of HDL (p = 0.02) with age. Dyslipidemia in type 2 DM patients at age group> 59 years old was shown by an increase in LDL level. The increase of TG and decrease of HDL level were significant in all age groups.Restriction of this study was not to check the antilipidemic medicine used. Early dyslipidemia of Type 2 DM should be known by lipidfraction determination and further dyslipidemia study should be conducted to predict the risk of CHD.
HIGH FLUORESCENT LYMPHOCYTE COUNT EXAMINATION IN DENGUE HEMORRHAGIC PATIENTS WITH SYSMEX XN-1000 HEMATOLOGY ANALYZER Budiono Raharjo; Solichul Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1443

Abstract

 Sysmex XN-1000 hematology analyzer is an automated 5-part diff analyzer (eosinophils, basophils, neutrophils, lymphocytes, and monocytes). In the calculated area, the type of difference between the Sysmex hematology device and other hematology devices is Immature Granulocyte (IG), Nucleated Red Blood Cell (NRBC), and High Fluorescent Lymphocytes Count (HFLC). The cells calculated in the HFLC area are atypical lymphocytes. In patients with dengue hemorrhagic fever, it is often found atypical lymphocytes called blue plasma lymphocytes. The purpose of this study was to determine the description of HFLC in patients with dengue fever using the hematology analyzer Sysmex XN-1000. A descriptive retrospective study was conducted during April-May 2017. The subjects of the study were adult patients diagnosed with dengue hemorrhagic fever with WHO criteria. Of the 47 samples of Dengue Hemorrhagic Fever (DHF) patients, the average HFLC results were between 2.0-32.3%, which was 11.5%, while the average range of normal HFLC values was between 0.0-1.4% and was 0.3%. In cases of DHF, there is an increase in HFLC. This is likely to be attributed to atypical lymphocyte increase in dengue hemorrhagic fever. Further research with more varied samples still needs to be done.
ASOSIASI HUMAN LEUKOCYTE ANTIGEN (HLA) KARSINOMA NASOFARING (KNF) F.M. Judajana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 2 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i2.946

Abstract

Nasopharyngeal Carcinoma (NPC) is one of the most frequent malignancy disease in Java and the incidence rate at several Hospitalsseems increasing yearly. Prevalence of NCP in Indonesian were 3.9 per 100.000 citizen each year. Eipsten-Barr Virus (EBV) is one of theetiological agents of Nasopharyngeal Carcinoma (NPC) and infected B lymphocyte that cause transformation of it to LymphoblastoidCell Line and expresses several antigens. One of them is known as Latent membrane Protein 2A (LMP2A). These antigens is the maintarget of Cytotoxic T lymphocyte (CTL) in immune system surveillance by recognizing an epitope Human Leukocyte Antigen (HLA)class I complexes which expressed on the target cell surface. Beside EBV, there are other factors that. Research of the HLA class I antigenis one of the immune genetic system that has the ability as genetic sensitivity to the disease. The research in NPC patients is not tobe done to show representative for of population in Indonesia especially in Java. The aim of the study was to know the associationbetween HLA class I profile and NPC patients in Java population and to isolate lymphocyte from peripheral blood 24 NPC patients formicrolymphocytotoxicity test with Terasaki Plate derived from UCLA-USA. The results is significantly associated to HLA – A 24 (RR 2.25),HLA A2 (RR 1.635) and HLA A11 (RR 1.065). Based on these HLA class 1 profile as an immune genetics marker on NPC is one of themost important target. In order to develop EBV vaccine in the future, this is necessary especially for Java Population in Indonesia.
OLD PEOPLE AND DIABETES MELLITUS Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.842

Abstract

Menjadi tua adalah proses mengembangkan hidup yang berkaitan dengan kumpulan perubahan yang di orang lanjut usia (lansia)dapat meningkatkan resiko kematian. Sindroma metabolik merupakan salah satu perubahan tersebut yang dikenal secara klinis danhasil laboratorik yaitu obesitas, hipertensi, intoleransi glukosa, dislipidemia, proinflammatory dan prothrombotic state. Hal ini dapatmeningkatkan prevalensi diabetes mellitus tipe 2 (T2DM) dan komplikasinya. Karena banyak kasus DM tidak terdiagnosis, penyaringan(skrining) yang diikuti pencegahan dan penatalaksanaan sangat diperlukan. Menyaring (skrining) orang lanjut usia dengan satu ataulebih komponen sindroma metabolik yang belum terdiagnosis DM dengan menilai glukosa darah puasa dan dua jam sesudah bebanuntuk diagnosis DM dan resiko kematiannya. Penyaringan (skrining) dilakukan di 1080 lansia umur 50 tahun atau lebih di Makassaryang diduga DM dengan tes glukosa plasma puasa dan dua jam sesudah beban, kemudian dinilai menurut klasifikasi WHO 1998.Dari 1080 pasien yang terdiri atas 560 (51,85%) laki-laki dan 520 (48,15%) perempuan, ditemukan DM, 420 (38,89%) denganglukosa plasma puasa  126 mg/dl, 425 (39,35%) dengan 2 jam sesudah beban glukosa  200 mg/dl dan 517 (47,87%) denganglukosa plasma puasa  126 mg/dl serta 2 jam sesudah beban  200 mg/dl. Satu atau lebih komponen sindroma metabolik orangtua merupakan biomarker atau prediktor untuk T2DM dan PJV. Pemeriksaan kesehatan secara reguler atau sedikitnya tes gula darahpuasa dan 2 jam sesudah beban glukosa di orang tua perlu dilakukan dan termasuk dalam sistem asuransi kesehatan. Diagnosis dinidiabetes dan penatalaksanaannya berarti mengurangi komplikasi, cacat, kematian maupun pembiayaan.
CORRELATION BETWEEN INTERFERON GAMMA RELEASE ASSAY OF ELISPOT METHOD AND CD4+ T LYMPHOCYTE CELL COUNT IN HIV POSITIVE PATIENTS Ambar, Nabil Salim; Aryati, Aryati; Kusmiati, Tutik; Triyono, Erwin Astha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1416

Abstract

Introduction. HIV is a virus that can cause AIDS, which affects the immune system and weakens the body function in fighting disease. The primary cells that HIV attacks are CD4+ T lymphocytes. Opportunistic Infections (OIs) are the biggest risk factors of death in HIV patients and occur in CD4+ T cells <200 cells/μL lymphocytes. TB is a disease with a high mortality rate in the world where Indonesia is a TB endemic country with the highest morbidity rates of TB in the world. The most common OI in people with HIV is TB. The number of limitations on Tuberculin Skin Test (TST) is large, thus in vitro T cells test with (Interferon Gamma Release Assay) IGRA is used in diagnosing latent TB. The aim of this study was to determine the correlation between IGRA ELISPOT method and CD4+ T lymphocyte cell count in HIV positive patients.Method. This was an observational analytical study with cross sectional design. The number of samples was 56 HIV positive patients who were treated at the UPIPI Clinic of the Dr Soetomo Surabaya Hospital. The examination of CD4+ T lymphocyte count was perfomed with FACSCalibur and IGRA was examined with T-SPOT.TB. The results were analyzed using Spearman correlation test.Results. CD4 + lymphocyte cell counts based on WHO groupings were as follows: > 500 cells / μL (33.92%), 200-349 cells / μL (25%), 350-499 cells / μL (25%) and <200 cells / μL (16 , 07%). IGRA examination results showed 35.18% positive and 64.81% negative. The grouping of CD4+ T lymphocyte cell counts based on IGRA test results was 27.77% with positive IGRA and 48.14% with negative IGRA. Spearman correlation test between CD4+ T cell lymphocytes with IGRA in HIV positive patients showed r = 0,036 (p = 0,794).Conclusion. There was no correlation between interferon gamma release assay of ELISPOT method and CD4+ T lymphocyte cell count in HIV positive patients.

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