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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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METABOLIC SYNDROME AMONG ADULTS IN RURAL AREAS (Sindrom Metabolik pada Dewasa di Daerah Pedesaan) Fenty Fenty; Widayati A; Virginia DM; Hendra P
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1241

Abstract

Sindrom metabolik merupakan sekumpulan tanda yang dicirikan dengan kondisi dislipidemia, peningkatan tekanan darah dankadar glukosa darah puasa serta kegemukan daerah perut. Sindrom metabolik telah menjadi salah satu masalah kesehatan di negaraberkembang. Penelitian menunjukan ada peningkatan prevalensi sehubungan sindrom metabolik, tetapi sebagian besar kajian dilakukandi daerah perkotaan.Tujuan penelitian ini adalah untuk mengetahui prevalensi sehubungan sindrom metabolik, di populasi dewasa yangberusia di atas 40 tahun di daerah pedesaan di Yogyakarta dengan cara menilainya. Penelitian ini menggunakan rancangan potonglintang yang dilaksanakan antara bulan Mei−Juni 2015 di Cangkringan Yogyakarta. Pengambilan data meliputi sosiodemografi melalui:wawancara, pengukuran antropometrik dan tekanan darah, serta kadar glukosa darah serta profil lipid. Responden penelitian terdiridari 50 laki-laki dan 50 perempuan dewasa usia ≥40 tahun. Batasan sindrom metabolik, menggunakan patokan International DiabetesFederation (IDF). Prevalensi sindrom metabolik berdasarkan patokan IDF di daerah pedesaan di Cangkringan Yogyakarta sebesar 25%.Sindrom metabolik, lebih banyak ditemukan di perempuan (32%) daripada laki-laki (18%). Komponen terbanyak dari sindrom metabolikadalah peningkatan tekanan darah di semua responden penelitian, kemudian diikuti dengan komponen kegemukan perut dan kadarHDL yang rendah di perempuan, sedangkan di laki-laki diikuti dengan hipertrigliseridemia. Prevalensi sindrom metabolik cukup tinggidi daerah pedesaan Yogyakarta khususnya bagi perempuan. Pencegahan terhadap sindrom metabolik menjadi keutamaan kesehatanmasyarakat dalam menurunkan penyakit kardiovaskular di daerah pedesaan di Yogyakarta.
Immature to Total Neutrophil (I/T) Ratio sebagai penunjang Diagnosis Sepsis Neonatorum Bastiana, Bastiana; Aryati, Aryati; Iriani, Yulia
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.967

Abstract

Early diagnosis of neonatal sepsis plays an important role in the management of patients. Blood culture, currently used as thegold standard, has several limitations such as time consuming and low positive rate. For this reason, a rapid and accurate diagnosticmethod is required. Manual differential count is a practical, inexpensive method and can support the diagnosis of bacterial infections.A shift to the left in differential white count with a raised immature neutrophil count has been documented in patients with bacterialinfections. This led to the use of I/T ratio as a indicator towards bacterial infections. The aim of this study is to obtain the diagnosticvalue of I/T ratio in diagnosing neonatal sepsis. The study was a prospective and cross-sectional. The subjects were enrolled consecutively,consisting of newborn babies (from birth to 30-days old) admitted to the Neonatal Intensive Care Unit (NICU) of the Dr. SoetomoHospital, Surabaya. Forty and three samples, consisting of 13 sepsis samples and 30 nonsepsis as controls samples were examined. I/Tratio are a ratio between immature neutrophils against total neutrophils in blood smear preparation. For the determination of the whitecell differential count, a total of 100 white cells (granulocytes) were counted. I/T ratio > 0.2 showed an abnormality that suggestaninfection process occur. Blood smear evaluations were done by three (3) independent observers. The result from three (3) observerswere as follows: sensitivity and specificity of I/T ratio in the diagnosis of neonatal sepsis were 69.2%, 92.3%, 61.5% and 50%, 50%,63.3%, respectively. Positive and negative predictive values were 37.50%, 44.44%, 42.10% and 78.94%, 93.75%, 79.16%, respectively.According to Cochran test there was no difference found between the 3 observers (p = 0.086). However, using Kappa test no agreementbetween I/T ratio and sepsis (p = 0.051) differences were found. from this study so far, the value in the diagnosis of neonatal sepsis theI/T ratio showed a low diagnostic.
DIAGNOSIS TIROID (Diagnosis of Thyroid) Liong Boy Kurniawan; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1285

Abstract

Thyroid disease often causes unspecific or mild symptoms, so laboratory tests are needed to confirm the functional diagnosis of thethyroid disorder. The laboratory tests which are important to establish the diagnosis of thyroid disorder include: total and free thyroidhormones, its related (thyroid) hormone binding proteins and auto antibodies. The thyroid hormone tests are mostly measured withcompetitive or sandwich immunoassays and each method can be interfered by several factors. Some drugs may increase or decrease thethyroid functional tests and several factors such as: underlying diseases, age, pregnancy, occurrence of heterophil antibody and autoantibodies may also interfere the thyroid tests results. The interpretation of an unusual combination from thyroid stimulating hormonesuch as free thyroxin and tri-iodothyronine results needs confirmation of underlying condition for establishing the right diagnosis. Thisreview is aimed to evaluate several factors which may influence the thyroid tests and interpretation.
PEMETAAN PERUBAHAN (MUTASI) VIRUS HEPATITIS B Tonang DA; Rina AS; JB Suparyatmo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 2 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i2.901

Abstract

Recently, the issue of Hepatitis B Virus (HVB) mutation is becoming a significant point to consider. The mutation might rendersignificant problems as the virus might escape from the detection method, vaccination induced-protection and treatment modalities. Thestudy was to analyze the profile of serological parameters in the HVB infected serum in Surakarta. As many as 36 HBsAg-positive serawere randomly retrieved from the patients in Moewardi Hospital and donors in Blood Bank of Indonesian Red Cross (PMI) in Surakartaduring August-September 2007. Having analyzed by immunoanalyzer, 13 (36.1%) of 36 sera were HBeAg-positive suggesting an activeinfection and potent of transmission. Interestingly, 3 (8.3%) of 36 sera were Anti-HBs-positive, while the other 5 (13.8%) showeddetected level of Anti-HBs even lower than the cut-off (12 mIU/ml). Accordingly, 22 (61.1%) of 36 serum were Anti-HBe-positive, whileone (2.8%) sera was HBe-Ag-positive as well as Anti-HBe-positive. The data suggested some possibilities: double infection with two ormore subtypes of HBV, mutation resulting in quasi-species phenomenon, or the so-called healthy carrier. More extensive and specificstudies are necessary to confirm and elucidate the profile.
CORRELATION OF URINE N-ACETYL-BETA-D-GLUCOSAMINIDASE ACTIVITY WITH URINE ALBUMIN CREATININE RATIO IN TYPE 2 DIABETES MELLITUS Melly Ariyanti; Lillah Lillah; Ellyza Nasrul; Husni Husni
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1207

Abstract

Urine Albumin Creatinine Ratio (UACR) merupakan metode untuk mengukur mikroalbuminuria, petanda awal dan paling banyakdigunakan untuk diagnosis nefropati diabetes. N-asetil-beta-d-glukosaminidase (NAG) air kemih adalah enzim lisosom dengan beratmolekul besar sehingga tidak difiltrasi oleh glomerulus. Ekskresi NAG di air kemih sangat mudah terganggu terutama oleh filtrasialbumin. Tujuan penelitian ini untuk mengetahui kenasaban NAG air kemih dengan UACR di pasien DM tipe 2. Penelitian ini merupakanpenelitian analitik dengan rancangan potong lintang terhadap 25 pasien DM tipe 2 yang berobat ke Poliklinik Endokrin Penyakit DalamRSUP Dr. M. Djamil Padang. Penelitian dilakukan mulai bulan Januari 2016–September 2016. Albumin air kemih diukur denganmetode imunoturbidimetri, kreatinin air kemih dengan metode Jaffe dan NAG dengan Enzyme-Linked Immune Sorbent Assay (ELISA).Kenasaban Spearman dilakukan untuk mengetahui hubungan aktivitas NAG air kemih dan UACR. Penelitian ini terdiri dari laki-laki68% dan perempuan 32%. Rerata umur 56,16±7,6 tahun dengan rentang 39–67 tahun. Kadar glukosa, ureum dan kreatinin serumberturut-turut, 148±49 mg/dL, 24±6,2 mg/dL dan 0,9±0,3 mg/dL. Normoalbuminuria sebanyak 80% dan mikroalbuminuria 20%.Median nilai UACR pasien DM tipe 2 adalah 6,02 (16,46) mg/g dengan rentang 1,53–119,41 mg/g dan rerata kadar NAG adalah51,01±31,88 ng/mL dengan rentang 9,45–144,38 ng/mL. Uji kenasaban Spearman menunjukkan kenasaban yang kuat antara aktivitasNAG air kemih dengan UACR dengan r=0,614 dan p<0,05. Terdapat kenasaban yang kuat antara aktivitas NAG air kemih denganUACR di pasien DM tipe 2.
PATTERN OF BACTERIA AND ITS ANTIBIOTIC SENSITIVITY IN SEPSIS PATIENTS (Pola Kuman dan Kepekaan terhadap Antibiotik bagi Pasien Sepsis) Wahyuni, Wahyuni; Nurahmi, Nurahmi; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1189

Abstract

Sepsis merupakan masalah serius karena berkebahayaan kematian yang tinggi. Penggunaan antibiotik profilaksis telah menjaditata langkah baku untuk penanganan sepsis, tetapi jika pola kuman dan kepekaan antibiotik tidak diketahui, maka akan memperbesarkemungkinan resistensi kuman terjadi yang selanjutnya akan menyebabkan peningkatan angka infeksi. Penelitian ini bertujuanmengetahui pola kuman dan kepekaannya terhadap antibiotik di pasien sepsis di RS Dr. Wahidin Sudirohusodo Makassar masa waktuJanuari 2009–Desember 2010. Metode penelitian yang digunakan adalah observational dengan desain potong lintang. Data penelitiandiambil dari hasil kultur dan kepekaan terhadap antibiotik yang tertulis di rekam medis pasien sepsis di RS Dr. Wahidin SudirohusodoMakassar masa waktu Januari 2009–Desember 2010. Data yang dikumpulkan kemudian dianalisis menggunakan metode statistiksebaran kekerapan. Telitian menunjukkan umur pasien sepsis terbanyak adalah di atas 40 tahun. Infeksi lebih banyak disebabkan olehbakteri negatif Gram (90,48%). Urutan kuman terbanyak adalah Alkaligenes faecalis (30,16%), Escherichia coli (12,70%), Acinetobactercalcoaceticus (12,70%) dan Staphylococcus aureus (7,94%). Uji kepekaan terhadap 39 jenis antimikroba, terdapat 12 antimikroba yangmemperlihatkan presentase resistensi yang tinggi (75% ke atas). Antimikroba yang paling peka yaitu Nitrofurantoin (83,33%). Dapatdisimpulkan bahwa bakteri yang terbanyak ditemukan di sepsis adalah Alkaligenes faecalis, Escherichia coli, Acinetobacter calcoaciticusdan Staphylococcus aureus. Antimikroba yang masih peka adalah Nitrofurantoin. Disarankan untuk dilakukan penelitian pola kumandan kepekaan terhadap antibiotik secara berkala.
EVALUASI PEMERIKSAAN IMUNOKROMATOGRAFI UNTUK MENDETEKSI ANTIBODI IgM DAN IgG DEMAM BERDARAH DENGUE ANAK Ety Retno Setyowati; Aryati Aryati; Prihatini Prihatini; M.Y. Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.850

Abstract

The gold standard diagnosis of DHF by RT-PCR needs a complex technology and is time consuming. Serological tests have beendeveloped to detect IgM and IgG anti dengue to determine primary as well as secondary acute phase infection. IgM and IgG antidenguetests by immunochromatography have been used, due to a high diagnostic validity, also because they are simple, practicable, easy, rapid(15–30 minutes), can be used in a single serum sample. ELISA method has been used as a confirmation method. The aim of this studyis to evaluate the immunochromatography method in detecting IgG and IgM anti dengue of DHF patients. The study was performedon 50 serum samples from patients of the ICU Department of Paediatrics Dr. Soetomo Hospital, Surabaya during July–August 2005with dengue virus infection according to the 1997, WHO criterion and 27 serum samples from non dengue virus infection patients.ELISA method showed positive infection in 44 samples. Immunochromatography method showed positive infection in 43 samples, butwas negative in 1 sample. Diagnostic sensitivity of Immunochromatography is 97.7% (43/44) and the diagnostic specificity is 92.6%(25/27). Immunochromatography method has a high diagnostic value in assisting the diagnosis of DHF.
COMPARISON OF GLYCEMIC STATE IN PATIENTS WITH AND WITHOUT HYPERURICEMIA Corrie Abednego; Banundari Rachmawati; Muji Rahayu
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1153

Abstract

Hiperurisemia merupakan keadaan asam urat yang meningkat dalam serum. Beberapa penelitian melaporkan hiperurisemia menyebabkankerusakan sel beta pankreas dengan mekanisme apoptosis melalui jalur NF-kB serta berhubungan dengan komplikasi mikrovaskular danmakrovaskular pada pasien diabetes. Perbedaan status glikemia (glukosa darah puasa/GDP dan glukosa darah 2 jam post-prandial/GD2PPdan HbA1c) pada pasien dengan dan tanpa hiperurisemia belum banyak diketahui. Tujuan penelitian untuk membuktikan perbedaan statusglikemia pada pasien dengan dan tanpa hiperurisemia. Penelitian retrospektif, 110 pasien yang dibagi menjadi kelompok hiperurisemia dantanpa hiperurisemia. Glukosa darah puasa dan 2 jam PP diperiksa menggunakan metode heksokinase, asam urat dengan metode urikase,HbA1c dengan metode elektroforesis kapiler. Data diuji normalitas data dan perbedaan antara variabel, dianalisis dengan uji Mann-Whitney.Subjek 58 laki-laki dan 52 perempuan, nilai rerata umur pasien 56,36 ± 8,7 tahun. Pasien laki-laki, terdapat perbedaan bermakna statusglikemia (GDP, GD2PP, HbA1c) terhadap kelompok hiperurisemia dan tanpa hiperurisemia, p< 0,05. Pasien perempuan, terdapat perbedaanbermakna status glikemia (GDP dan GD2PP), p< 0,05 serta HbA1c tidak terdapat perbedaan bermakna pada pasien hiperurisemia dan tanpahiperurisemia dengan p=0,084. Terdapat perbedaan bermakna pada status glikemia pasien laki-laki dan perempuan terhadap kelompokhiperurisemia dan tanpa hiperurisemia, kecuali HbA1C pada perempuan. Dibutuhkan penilaian terhadap diet pasien yang dapat mempengaruhikadar asam urat.
ANALISIS KING’S SCORE DI PENYAKIT HATI KRONIS BERDASARKAN FIBROSCAN Wira Wira; Amaliyah T. Lopa; Ibrahim Abdul Samad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1121

Abstract

Chronic liver disease is a commonly found disease in Indonesia and remains a serious health problem. Chronic liver disease oftenprogresses to hepatic cirrhosis or hepatoma initiated by fibrotic process in liver. Liver biopsy result is a gold standard in determining theliver fibrosis degree, but it has some limitations. King’s Score is a non-invasive method that uses routine laboratory predictors relatedto liver fibrosis. Therefore, it is necessary to know the diagnostic value of King’s Score in determining the fibrosis degree of liver chronicdisease by using fibro scan as the gold standard in stead of biopsy. This study was a retrospective study with data collected from themedical records from the Medical Record Installation at the Dr. Wahidin Sudirohusodo General Hospital between January 2011 up toDecember 2013. The study results derived from 99 samples with Spearman correlation test showed a significant relationship between theKing’s Score and fibrosis degree of chronic liver disease (p=0.000 and r=0.651). The analysis of the receiver operating characteristics(ROC) indicated that in mild and moderate fibrosis the AUC value was too weak, whereas in heavy fibrosis the AUC value was 0.860,which was statistically very strong with a cut-off value of 7.15 and with a 80.6% sensitivity, and a 75.0% specificity, Positive PredictiveValue59.5%, Negative Predictive Value 89.5%. This meant that the King’s Score value of >7.15 could be used as a heavy fibrosis degreepredictor in chronic liver patients. Further studies are needed to analyze the King’s Score based on chronic liver disease etiology and usea liver biopsy as the gold standard in determining the degree of liver fibrosis.
KEPUASAN PELANGGAN INTERNAL Rosni Faika; O Sianipar
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 1 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i1.925

Abstract

Customer satisfaction survey is one of efforts of continuously quality improvement of care and also can be used to maintain customers that already utilize available services. Efforts of corrective action should be done based on result of this survey. The objective of this surveyis to assess desired-service and reality of the service undergone by internal customer and to improve quality of service of laboratorymedicine in Clinical Laboratory of Dr. Sardjito Hospital, Yogyakarta. This is cross sectional customer satisfaction survey. Subject of thissurvey are 23 doctors work in Dr Sardjito Hospital. Data collected by questionnaire that contain 18 items about desired-service andreality of service undergone by internal customer from service given by Clinical Laboratory of Dr. Sardjito Hospital. Respondents assessdesired-service and reality of service using Likert scale. The obtained data are analyzed by Cartesius diagram. Result of the survey showsthat average concordant between desired-service and reality of service is 85.5%. Main priority corrective action to improve quality ofservice are: 1) readiness of registry staff; 2) easiness of registration procedure; 3) skill of laboratory staff in explaining and recordingtest parameter; 4) service of cito service; 5) 24 hours laboratory service and 6) timely laboratory test.

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