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Dr. dr. Puspa Wardhani, SpPK
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admin@indonesianjournalofclinicalpathology.org
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+6285733220600
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majalah.jicp@yahoo.com
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Laboratorium Patologi Klinik RSUD Dr. Soetomo Jl. Mayjend. Prof. Dr. Moestopo 6-8 Surabaya
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Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Articles 1,328 Documents
THE HEMOGLOBIN, RDW, AND MEAN CORPUSCULAR VALUES IN PATIENTS WITH BETA-THALASSEMIA/HEMOGLOBIN E DISEASE AND BETA-THALASSEMIA TRAIT Vinisia Setiadji; Bidasari Lubis; Adi Koesoema Aman; Herman Hariman
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1459

Abstract

Thalassemia beta / hemoglobin E adalah suatu kondisi dengan heterozigot ganda gen pembawa thalassemia beta dan hemoglobin E. Hal ini menyebabkan kondisi dengan gambaran fenotip yang berat dibandingkan trait thalassemia beta dan trait hemoglobin E. Secara logika, nilai mean corpuscular dari thalassemia beta / hemoglobin E seharusnya memburuk. Pada penelitian ini, kami meneliti sebelas kasus dari dua keluarga dengan anggota menderita thalassemia beta / hemoglobin E.Pada keluarga-1 dua anggota dengan trait thalassemia beta memiliki nilai MCV 68 fL dan 65 fL, dan nilai MCH 21 pg dan 20 pg. Pada keluarga-2 anggota dengan trait thalassemia beta memiliki nilai MCV 60,2 fL dan MCH 18,8 pg. Anak perempuan dari kedua keluarga dengan thalassemia beta / hemoglobin E memiliki nilai mean ± SD MCV 70,8 ± 4,9 fL dan MCH 22.8 ± 2.3 pg, nilai ini signifikan lebih tinggi daripada trait thalassemia beta (p<0.05). Terdapat perbedaan yang signifikan antara nilai hemoglobin dan RDW antara thalassemia beta / hemoglobin E (p=0.001).Kami juga menemukan bahwa nilai MC dari keadaan post-transfusi signifikan lebih tinggi daripada pre-transfusi (p<0.001)Kami menyimpulkan bahwa nilai MC dari thalassemia beta / hemoglobin E secara persisten lebih tinggi daripada trait thalassemia beta. Peran transfusi darah pada pasien dengan thalassemia beta / hemoglobin E tampak memainkan peran dalam diskrepansi pada kasus ini.
ANTI HCV DAN JUMLAH PENDERITA JANGKITAN (PREVALENSI INFEKSI) VIRUS HEPATITIS C Isti Setijorini Wulandari; Kismardhani .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.993

Abstract

ABSTRACTIn various prevalence Hepatitis C Virus (HCV) infections was found all over the world. There is no accurate data about the spreading of HCV infection in Indonesia. Indonesian Department of Health makes a program to make a database from all provinces in Indonesiaabout prevalence of HCV. This is supported by request from the district Health Department of Daerah Istimewa Yogyakarta (DIY)Province to conduct HCV infection surveillance in 2007. Data of HCV infection from blood transfusion service show approximately 2%.To complete the basic data by knowing the prevalence of HCV in Dr. Sardjito General Hospital Yogyakarta at DIY province. This is an Hospital Yogyakarta at DIY province. This is anHospital Yogyakarta at DIY province. This is an observational descriptive study about HCV infection prevalence in Dr. Sardjito General Hospital Yogyakarta. Data was collected fromSeptember 2007−August 2008 conducted on Microbiology, Immunology & Infection Sub Laboratory of Clinical Pathology Departmentin Dr Sardjito General Hospital Yogyakarta. There was 753 request of anti HCV test from September 2007−August 2008. Initial reactiveresult was found in 131 patients. In the first quarter in 2007 (January−April 2007) there were 27 initial reactive from 175 samples ofanti HCV test (15.42%). In the second quarter were found 36 initial reactive out of 176 samples of anti HCV test (20.45%) and in thelast trimester in 2007 were found 19 initial reactive out of 134 samples (14.17%). In 2008, first quarter were found 21 positive resultout of 123 samples (17.03%) and in the second quarter (May−August 2008) were found 32 positive result out of 145 samples (22.06%).There are gradually increased percentage rates of initial reactive anti HCV test based on the result from each trimester HCV test based on the result from each trimesterHCV test based on the result from each trimester (15.42−20%, 45−14 %, 17−17%, 03−22.06%)
NILAI DIAGNOSTIK UJI TROPONIN I KUANTITATIF METODE IMMUNOKROMATOGRAFI Siti Fatonah; Anik Widijanti; Tinny Endang Hernowati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 1 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i1.920

Abstract

Cardiac troponins are the most sensitive and specific biochemical markers of myocardial damage but there is no standardization of WHO for cardiac troponin I, resulting in a variability for diagnostic value. It is necessary to determine diagnostic value for a new kitof troponin I. To evaluate a new quantitative immunochromatography assay for troponin I at a various cut off level. A cross sectionalstudy was conducted in 64 patients with acute myocardial infarction (AMI) and 55 non-AMI as control from February to September2007. The level of cardiac troponin I (cTnI) was measured and determined it diagnostic value at a various cut off level. The sensitivity,specificity, PPV and NPV of this assay were 91%, 91%, 92% and 89% at cut off level of 1,0 ng/ml (according to the kit), respectively.The cut off of cTnI were divided into five levels: 0.8, 1.0, 1.2, 1.5, and 2.0 with the area under curve were 0.923, 0.908, 0.912, and0.897, respectively. The sensitivity were 94%, 91%, 86%, 81% and 72%, respectively, the specificity were 91%, 91%, 96%, 98% and98%, respectively. This rapid diagnostic test is sensitive and specific to diagnose an acute coronary syndromes.
NEOPTERIN DAN PEROKSIDA SERUM SEBAGAI PETANDA MAKROFAG TERAKTIVASI PADA TUBERKULOSIS PARU AKTIF DAN INDIVIDU BERKEBAHAYAAN TINGGI (Serum Neopterin and Peroxide As Marker of Activated Macrophages on Active Pulmonary Tuberculosis and Individuals At High Risk) I Nyoman Wande; Ni Made Linawati; I Made Bagiada; IWP. Sutirta Yasa; AAN. Subawa
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 1 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i1.1227

Abstract

Failure of macrophages to phagocytize Mycobacterium tuberculosis causes the release of hydrogen peroxide/peroxide (H2O2) bythe activated macrophages. Neopterin is one of the most important markers in the activated macrophages. Neopterin is a pteridinederivative produced by the activated macrophages through the stimulation of interferon gamma. Increased levels of Neopterin has beenreported in lung tuberculosis. Activation of macrophages is essential to the development of tuberculosis infection that can lead to activepulmonary tuberculosis or latent tuberculosis, in this case is a high-risk for healthy individuals. To determine the differences in serumlevels of Neopterin and H2O2 between patients with active pulmonary tuberculosis and healthy individuals at high risk of pulmonarytuberculosis. A total of 15 patients with active pulmonary tuberculosis and 15 healthy individuals at high risk examination serumNeopterin levels and peroxide (H2O2). Active pulmonary tuberculosis patients when the results of sputum smear examination chest x-raysis obtained positive results. Healthy individuals at high risk when the results of smear examination and chest x-rays is negative. Thelevel of Neopterin was examined using a double antibody sandwich immunoassay with Human neopterin (NEOP) ELISA Kit ® BioassayTechnology Laboratory. The level of peroxide was examined using quantitative colorimetric peroxidedetermination with QuantiChromTMPeroxide Assay Kit (DIOX-250)®. Neopterin serum levels between patients with active pulmonary tuberculosis and healthy individualsat high-risk were analysed by independent samples t-test. H2O2 serum levels between patients with active pulmonary tuberculosis andhealthy individuals at high-risk were analysed with Mann Whitney Test. The confidence level is p <0.05. The mean Neopterin levelsin patients with active pulmonary tuberculosis was 5.17±4.64 nmol/L, the mean Neopterin levels in group of healthy individuals athigh risk was 3.97±1.79 nmol/L. Statistical analysis by the independent samples t-test found no significant differences between groupsin Neopterin serum levels of patients with active pulmonary tuberculosis and healthy individuals at high risk (p=0.357). The meanserum levels of H2O2 of group of patients with active pulmonary tuberculosis was 26.38±3.00 μM, the mean levels of H2O2of group ofhealthy individuals at high risk of 20.69±4.46 μM. Statistical analysis with non-parametric Mann-Whitney Test was found significantdifference in the peroxide (H2O2) levels between groups of patients with active pulmonary tuberculosis and group of healthy individualat high-risk (p=0.000). The levels of Neopterin in patients with active pulmonary tuberculosis was not significantly higher comparedto the healthy individuals of high risk. Levels of peroxide (H2O2) serum in patients with active pulmonary tuberculosis was significantlyhigher compared to the group of healthy individuals at high risk. This shows that there is increased activity of macrophages in patientswith active pulmonary tuberculosis, but not effective in eliminating of Mycobacterium tuberculosis.
MONOCYTE LYMPHOCYTE RATIO IN DENGUE HEMORRHAGIC FEVER Dwi Retnoningrum; Purwanto AP
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1130

Abstract

Dengue Hemorrhagic Fever (DHF) adalah penyakit yang disebabkan oleh virus dengue, virus yang dapat ditularkan melalui gigitannyamuk. Dengue hemorrhagic fever merupakan salah satu penyebab kesakitan dan kematian terbanyak di dunia termasuk di Asia.Patogenesis infeksi DHF diduga melibatkan monosit dan limfosit akibat dari respons imun terhadap infeksi. Monocyte Lymphocyte Ratio(MLR) sebelumnya digunakan dalam menggambarkan respons imun di infeksi malaria, tuberkulosis dan HIV. Penelitian ini bertujuanuntuk mengetahui apakah terdapat perbedaan MLR di infeksi DHF derajat ringan dan berat. Metode penelitian ini observasional analitikdengan desain potong lintang di pasien DHF di RS Dr. Kariadi Semarang masa waktu Januari-Desember 2013. Nilai MLR didapat dariperhitungan jumlah monosit dibagi jumlah limfosit dari hitung jenis lekosit. Derajat DHF ditentukan sesuai dengan patokan WHO, yaituderajat I-II masuk dalam derajat ringan, derajat III-IV adalah derajat berat. Analisis statistik dengan Student t test. Kelompok I terdiridari 40 pasien DHF derajat ringan dan kelompok II terdiri dari 40 pasien DHF derajat berat. Subjek terdiri dari 41 laki-laki (51,2%)dan 39 perempuan (48,8%). Rentang nilai MLR di DHF derajat ringan ditemukan dari 0,03–0,33 (median 0,11) sedangkan di DHFderajat berat dari 0,03–0,59 (median 0,16). Analisis statistik menunjukkan tidak terdapat perbedaan nilai MLR antara kelompok I(derajat ringan) dan kelompok II (derajat berat) (p=0,08). Tidak didapatkan perbedaan nilai MLR di infeksi DHF derajat ringan danberat.
KESAHIHAN DIAGNOSTIK HEMOGLOBIN RETIKULOSIT UNTUK DETEKSI DEFISIENSI ZAT BESI DI KEHAMILAN (Diagnostic Validity of Reticulocyte Hemoglobin for Iron Deficiency Detection in Pregnancy) Tri Ratnaningsih; Budi Mulyono; Sutaryo Sutaryo; Iwan Dwiprahasto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1282

Abstract

Entering the second trimester of pregnancy, more iron is required due to the increase in erythrocyte mass, plasma volume andthe development of fetus as well as chorion. Iron is needed the most in the third trimester. The existing hematological iron stageparameters can only detect iron deficiency in the latest stage. The aim of this study was to know the assessment validity of Ret-Heexamination as a new parameter to diagnose iron deficiency in pregnant women with anemia, as well as a screening tool for those interm pregnancy without anemia. The research design was cross sectional. The subjects were women in term pregnancy, gathered fromPKU Muhammadiyah Hospital, Bantul Yogyakarta from May to November 2013. A seven (7) mL blood sample was taken from thecubital vein of the subjects. Two mL of the sample was tested for routine hematological examination using an EDTA tube, while theRet-He was assessed using an automatic hematological instrument Sysmex XT-2000-i (Symex Corporation, Kobe, Japan). The serumof the remaining five (5) mL was used to check the serum iron and TIBC to obtain the saturation value (Tsat) using Cobas analyzerC501 (Roche Diagnostics, Germany), while the serum ferritin (SF) was examined using Minividas. The subjects were classified into two(2) groups based on the Hb levels, namely: anemia (Hb<11 g/dL) and those who did not (Hb≥11 g/dL). Furthermore, they were alsoclassified into two (2) groups based on transferrin saturation values: iron deficient (Tsat <9%) and normal (Tsat ≥9%). From 291subjects, 59 (20.3%) were found to have anemia and 232 (79.7%) did not. The cut off value of Ret-He to diagnose iron deficiency inpregnant women with anemia was 29.8 pg (82% sensitivity and 72% specificity). Meanwhile, the cut-off value of Ret-He for irondeficiency screening in pregnant women without anemia was 29.8 pg, with a sensitivity and specificity of 92% and 87% respectively.The Ret-He holds a good diagnostic validity to detect iron deficiency in pregnancy, with or without anemia.
STATUS PENGGUMPALAN (AGREGASI) TROMBOSIT SEBAGAI FAKTOR PROGNOSTIK TEJADINYA KELUARAN KLINIS STROK INFARK MENDADAK (STROK INFARK AKUT) Linda Rosita; Usi Sukorini; Budi Mulyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1021

Abstract

The propriate management of acute infarction stroke will be able to reduce the morbidity and mortality of the disease. In diagnosingand managing the diseases, for the detection of the risk or prognostic factors information’s such as the history physical findings,confirmation and other supporting tests are needed. One of the supporting test is the laboratory examination i.e. platelet aggregationtest. Platelet aggregation is suggested having an important role in haemostasis especially to prevent excessive bleeding by forming plateletplug. Finally, further hyperactivity of platelet in terms of platelet hyper aggregation can create a thrombus and moreover lead to obstructthe vessels. The occlusion will give a negative outcome of an acute infarction stroke. The aim of this study is to know whether platelethyper aggregation has a contribution in the outcome of the disease or not by certain testing. A prospective cohort study was carried out,to compare between two groups of exposed and non-exposed group in Sardjito hospital Yogyakarta during the period of March up toearly July 2007. Eighty four subjects who met the inclusion and exclusion criteria were involved in this study. The exposed group was agroup of acute infarction stroke patient who were exposed to platelet hyper aggregation 48 (57%), on the other hand, patients who didnot have platelet hyper aggregation was separated as non-exposed group is 36 (43%). Inception of cohort was applied when the patientwas admitted to the emergency unit during 72 hours of the onset, before receiving antithrombotic drugs and had no previous history ofstroke. The patients were followed after 7 days hospitalization in the stroke unit and neurology unit and the outcome was measured byevaluating the score using Gadjah Mada stroke scale. The characteristics of the subjects were grouped by baseline data X2 test. Unvariedanalysis and multivariate analysis were taken to get the relative risk of having acute infarction stroke. In this case logistic regressionanalysis was used to know the relative risk (RR) measurement. Prognostic factors had influenced the outcome of acute infarction strokein patients who had a history of cardiovascular disease and aggregation status. The outcome of the platelet hyper aggregation grouphad a RR=2.15 (95% CI: 2.01–4.07) and the history of cardiovascular disease had a RR=1.78 (95% CI: 1.18–13.28).
KENASABAN FIBRINOGEN PLASMA DENGAN PENEBALAN ARTERI INTIMA-MEDIA KAROTIS KOMUNIS DI DIABETES MELITUS (Correlation Plasma Fibrinogen With Intima-Media Thickness of Carotid Artery in Diabetes Mellitus) Dwi Aryani; Budi Mulyono; Osman Sianipar
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1250

Abstract

Diabetes mellitus is a global problem due to the increase of its prevalence, morbidity and mortality. Diabetes mellitus has chroniccomplications such as : micro vascular and macro vascular. The macro vascular complications reveal as atherosclerosis. The increase ofcarotid communist IMT was examined by using USG, which has become the standard for assessing atherosclerosis and recommended by theAmerican Heart Association. Fibrinogen levels have a role in atherosclerosis. This study was design cross-sectionals to know the associationbetween plasma fibrinogen with carotid communist IMT in patients with DM by special examination. The subjects were patients with DMcoming to Dr DR. Sardjito Yogyakarta and fulfil the inclusion as well as the exclusion criteria. The examination of fibrinogen was carried out by Claus’s method. The result showed positive correlation between plasma fibrinogen with carotid communist IMT in type 2 diabetes mellitus, r = 0.36 and p = 0.021. There is also positive correlation between age and carotid IMT with r = 0.314 and p = 0.048.
THE DIFFERENCE BETWEEN NEUTROFIL TOTAL, NEUTROPHIL / LYMPHOCYTE AND PLATELETS / LYMPHOCYTE RATIO IN NORMAL PATIENTS, NSTEMI, STEMI Elisabeth Setianingrum; Purwanto A P
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1445

Abstract

Sindrom Koroner Akut(SKA) yaitu Non ST Elevasi Miokard Infark (NSTEMI) dan ST Elevasi Miokard Infark (STEMI) disebabkan oleh aterosklerosis pembuluh darah, merupakan proses inflamasi akut diawali kerusakan endotel. Neutrofil Absolut (NA), Neutrofil/Limfosit Ratio (NLR), Platelet/ Limfosit Ratio (PLR) merupakan petanda inflamasi sistemik pada penyakit inflamasi. Tujuan untuk membandingkan NA, NLR dan PLR sebagai petanda inflamasi pada pasien normal, NSTEMI dan STEMI . Penelitian observasional analitik dengan pendekatan belah lintang terhadap 101 pasien SKA di RSU Negeri yang terbagi menjadi 3 kelompok (normal, NSTEMI, STEMI). Leukosit, platelet dihitung dengan hematology analizer, limfosit dan neutrofil dengan hitung jenis . Analisis data menggunakan uji Kruskal Wallis  dilanjutkan uji post hoc Mann-Whitney. Terdapat perbedaan NA,NLR pasien normal dengan NSTEMI dan pasien normal dengan STEMI (p=0,000). Tidak terdapat perbedaan PLR pada pasien normal dan NSTEMI, pasien normal dan STEMI, NSTEMI dan STEMI. Neutrofil Absolut dan Neutrofil/Limfosit Ratio sebagai faktor independent yang baik untuk petanda inflamasi sistemik menggambarkan prognosis penyakit.
PETANDA PERADANGAN hs CRP DENGAN HIPERTENSI Suswanto .; Siti Muchayat P
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.976

Abstract

The major pathophysiologic mechanisms of hypertension include the activation of sympathetic nervous system and rennin–angiotensin–aldosterone system. Endothelial dysfunction, increased vascular reactivity, and vascular remodeling may cause that matter,rather than its consequences, of blood pressure elevation; increased vascular stiffness, which contributes to isolate systolic hypertensionin the elderly persons. Chronic low grade inflammation has been identified as an integral part in the pathogenesis of vascular disease.Inflammation may also be implicated in the development of hypertension. Several studies have demonstrated a significant relationshipbetween hs-CRP and stiffness of large arteries. If inflammation is truly linked to hypertension, then it would be reasonable to assume thatdrugs with anti-inflammatory actions may be able to arrest inflammation, improve endothelial function and lowering blood pressurein patients with hypertension. The aim of this cross sectional design study is to observe high sensitive C-reactive protein (hs-CRP)inflammatory markers in thirty five patients with hypertension. also want to know the present correlation between marker inflammationhs CRP with hypertension. With examine to some confounding factors as LDL, GDR and WBC. The results of this study showed that thereis no correlation between hs-CRP and hypertension, especially the duration of hypertension (r = 0.5 and p = 0.002). High degree ofhypertension was not followed by the increasing level of hs CRP.

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