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Journal of Parasite Science

JoPS
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Published by Universitas Airlangga

ISSN : 25990993 EISSN : 26565331 DOI : https://doi.org/10.24073

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

Journal of Parasite Science (JoPS) publishes the results of original research in all aspects of basic and applied parasitology, and ranging from parasites biodiversity, parasites of all wildlife, invertebrate and vertebrate, as well as host-parasite relationships of intrinsic biological interest to those of social and economic importance predominately in veterinary, human medicine and agriculture aspect. Original research includes the development of novel and innovative concepts and ideas, as well as experimental and observational science that raises new theory.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 136 Documents

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Identifikasi Protozoa pada Darah dan Saluran Pencernaan Biawak Air (Varanus salvator) Azizah Bilqis Nurkarimah; Mufasirin Mufasirin; Ratna Damayanti; Lucia Tri Suwanti; Boedi Setiawan; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research is to identify the variaous of protozoa in the bloodanddigestive tract on water monitor lizard (Varanus salvator) was captured in Sidoarjo, East Java. This research was taken on March until June 2019. This research used a non-experimental method through an observation study. As many as 50 water monitor lizard were used this research and examined at the Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga.The samples of this research were made in thin blood smear using Giemsa stain, while fecal examination using native method, sucrose flotation, and modified Ziehl-Neelsen stain.The results showed that two various of protozoa found were single infection. Observations on the blood was obtained Haemogregarina sp. (14%) and observations of feces obtained Eimeria sp. (2%). The conclusion of this research indicate that type of protozoa was Haemogregarina sp. and Eimeria sp. were found on water monitor lizard(Varanus salvator).

Anthelmintic Potential Extract Mango Gadung Seed (Mangifera indica L.) Mecistocirrus digitatus in vitro Ria Nikmatul Jannah; Sri Mumpuni Sosiawati; Sri Chusniati; Kusnoto Kusnoto; Rahmi Sugihartuti; Setiawati Sigit
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aim to attest the potential anthelmintic extract mango seed gadung (Mangifera indica L.) against worms Mecistosirrus digitatus in vitro, the study also aims to determine the effect of immersion time, variasis concentration and the relationship between time and treatment. this study used 210 M. digitatus worm extracted regardless of gender worms. treatment that is given was the concentration extract mango seed gadung was 5%, 7.07%, 10%, 14.14%, 20%, negative control using NaCl physiological and comparison using levamisole Hidrokloride 0.0025 mL. Observations death worm Mecistocirrus digitatus and observations were made at the 6th hour, 12th hour, 18th hour and 24th hour. The results showed that variations in concentration, soaking time and the relationship between soaking time with the treatment affect mortality Mecistocirrus digitatus worms. Test results of the analysis statitistik using factorial Anova and Duncan's Multiple Range Test showed a significant difference (p<0.01) between the time factor, variations in concentration and relations between time and treatment.

Prevalence and Nematode Infection Level on Gastrointestinal Tract at Horse (Equus caballus) in Bangkalan Madura Elok Apriliawati; Mufasirin Mufasirin; Wurlina Wurlina; Poedji Hastutiek; Lucia Tri Suwanti; Benjamin Christoffel Tehupuring
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study is to determine the prevalence, infection level, sex and age effect on the infection level of GIT Nematode parasite in a horse. The fecal samples analyzed using the methods of native, sedimentation, floatation, and worm eggs count per gram of feces. Sample examination found 54 positive infected gastrointestinal nematode parasite with prevalence rate of 87% (54 from 62). The prevalence of Trichonema sp., Strongylus sp., and Parascaris equorum are 37.1%, 16.1%, and 1.6%. There were also mixed infestation like Strongylus sp. and Trichonema sp; Strongylus sp. and Parascaris equorum; Trichonema sp. and Parascaris equorum with total prevalence 27.4%, 1.6%, and 3.2%. Sex and age of horse had a very significant and significant effect in prevalence and infection level of Nematode parasite.

The Prevalence of Gastrointestinal Tract Helminthiasis Through Stool Examination in Cattle at Benowo Landfill Surabaya Ratih Prajnya Paramitha; Rahaju Ernawati; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 1 No. 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Tujuan penelitian ini adalah untuk mengetahui prevalensi cacing, untuk mengetahui berbagai jenis telur cacing saluran cerna dan jenis umur terhadap infeksi cacing saluran cerna pada sapi di tanah Benowo, Surabaya. Sampel tinja diambil dari TPA sebanyak 41 sampel. Pemeriksaan tinja dilakukan dengan metode asli, sedimentasi sederhana dan pelapisan Fulleborn. Hasil penelitian menunjukkan bahwa 30 sampel positif menentang gastrointestinal, ini menunjukkan prevalensi cacing adalah 73%. Jenis telur cacing adalah kelas Nematode, yaitu Oesophagostomum sp., Trichostrongylus sp., Bunostomum sp. , Mecistocirrus digitatus, Trichuris sp dan Toxocara vitulorum.Menurut analisis statistik, dapat disimpulkan jenis dan jenis kelamin tidak dapat dihitung terhadap infeksi cacing.

The Effect of Folic Acid as Supportive Therapy of Spiramycine on Weight of Foetus to Toxoplasma gondii – Infected Pregnant Mice (Mus musculus) Alfina Azkiana; Boedi Setiawan; Erma Safitri; Lucia Tri Suwanti; Mufasirin Mufasirin; Djoko Legowo
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aimed to investigate the effect of folic acid as supportive therapy of spiramycine on weight of foetus to Toxoplasma gondii-infected pregnant mice (Mus musculus). Twenty pregnant female mice were divided into four groups as C -, C +, T1 and T2. C +, T1 and T2 were infected by Toxoplasma gondii. C – and C + administered orally 0.5 ml aquadest, T1 administered orally 130 mg/kg BW spiramycine and 0.052 µg/g BW folic acid and T2 administered orally 0.052 µg/g BW folic acid. Experimental groups received the treatments for 5 days, then animals of each groups were sacrified. Foetuses were dissected out for observation. The weight of fetuses were measured using an analytical balance. The data weight of foetuses was presented descriptively and analyzed by ANOVA test and continued by Tukey HSD. From this study, the weight of foetuses from the pregnant mice of T1 and T2 have difference compared with the controls. The result of this research is folic acid affects the weight of foetuses to Toxoplasma gondii-infected pregnant mice.

Prevalence of Protozoa in Gastrointestinal Tract of Pigeons (Columba livia) Maintenance Ekstensif and Intensif in Surabaya Felita Widyaningsih; Rimayanti Rimayanti; Setiawan Koesdarto; Lucia Tri Suwanti; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

This research was conducted to determine the prevalence of major gastrointestinal protozoal infections, the kinds of protozoa that infects and maintenance systems influence on pigeons in Surabaya. Pigeon taken from five regions of Surabaya namely North Surabaya, East Surabaya, West Surabaya, South Surabaya and Surabaya Center. Each region is taken as many as 20 pigeons consisted of 10 pigeons with extensive maintenance systemÂ and 10 pigeons with intensif maintenance system. So that the total overall sample is 100 pigeons. Laboratory examination (microscopic) was conducted in November 2017 until January 2017. Examination of the upper gastrointestinal tract using throat swabs and stool samples were examined with native methods, sedimentation, and flotation. Identification using a microscope using 40x objective scale and 10x oculer scale to determine gastrointestinal protozoal that was found. Data was analyzed using Chi Square Test statistical analysis. Results showed 78% of the positive samples gastrointestinal protozoa infections in pigeons in the city of Surabaya. Results of Chi Square Test statistical analysis showed a p value of 0.030 (p<0.05) which means significantly different. Types of protozoa found that Trichomonas gallinae (49%) and Eimeria sp. (61%). The prevalence of gastrointestinal protozoal pigeons on extensive maintenance sistemÂ was higher (44%) compared to the intensive caresis system(34%).

Anthelmintic Activity Ethanol Extract of Ocimum sanctum Linn. Leaves Against Ascaridia galli In Vitro Vanna Lidya Kharisma; Setiawan Koesdarto; Koesnoto Supriandono; Lucia Tri Suwanti; Sri Agus Sudjarwo; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this research are to determine concentration, exposure time, interaction between concentration and exposure time of ethanol extract of Ocimum sanctum Linn. Leaves to cause death toward Ascaridia galli in vitro, and the value of LC50 and LC90 ethanol extract of Ocimum sanctum Linn. Leaves. Research design that has been used in the research was completely randomized design. This research used 200 samples of Ascaridia galli with length 7-11 cm without differentiating their sex. The concentration ethanol extract of Ocimum sanctum Linn. leaves were 1.25%, 2.5%, 5%, 10%. The control was using CMC-Na 0.5%. Each treatment then being replicated four times. The observation and recording of dead worm were done at 0, 3, 6, 12 and 24 hours. Ascaridia galli were declared dead if there was no movement while disturbed by anatomy tweezers and dipped in slightly warm water (50ºC). The obtained data was analyzed using Anova Factorial and continued with Duncan Multiple Range Test by SPSS for Windows 22. The result were 10% concentration and exposure time for 24 hours caused the most mortality toward Ascaridia galli. Interaction between concentration and exposure time resulted 10% concentration ethanol extract of Ocimum sanctum Linn. leaves in 24 hours caused the most mortality towards Ascaridia galli. Probit analysis was used to calculate the LC50 and LC90 of Ocimum sanctum Linn. leaves. The results were LC50 ethanol extract of Ocimum sanctum Linn. leaves at 6 hours was 14.8%, at 12 hours was 4.8% and at 24 hours was 3.0% and the LC90 at 24 hours was 9.1%.

Gambaran Jumlah dan Hitung Jenis Leukosit Ayam Petelur yang diinfeksi L2 Toxocara Cati Diyah Ayu Candra; Nunuk Dyah Retno Lastuti; Nove Hidayati; Kusnoto Kusnoto; Poedji Hastutiek; Retno Bijanti
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study was to determine leukocytes and differential counting in chicken after infected with L2 Toxocra cati. In this study, it was used twenty chickens, wich were 14 weeks old. They were divided into 4 groups. Chickens were infected by orally with dose 0 eggs/ml L2 T. cati, 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. Blood sampling were conducted on 2, 7 and 21 days after infection. Leukocyte value was determined by improve neubauer and differential counting stained with Wright’s stain then determined using microscope 1000x. The data was analyzed by Anova Factorial and then continued by BNJ Test at 5%. The result showed that increased of leukocytes value and eosinophil at 7 and 21 days after infection L2 T. cati with different dose, increased of leukocytes value and eosinophil on infection dose 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. It shows that there was not intereraction between time of infection process and infection dose of L2 T. Cati.

Effect of Garlic (Allium sativum) Infusion On The Time of Death of Ascaridia galli Ida Yuniarti; Suzanita Utama; Setiawan Koesdarto; Lucia Tri Suwanti; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Infectious disease is a major problem for animal husbandry. One of infectious disease is caused by Ascaridia galli (A. galli). Ascaridiasis occurs in either broiler or layer that caused decrease of meat and egg productions. Garlic (Allium sativum) contain alicinÂ that have the ability to kill parasites.This research was conducted to determine the effect of garlic on time of death of A. galli. In this research used A. galli with length 7-11 cm regardless the sex. The treatment used local (Indonesian garlic), kating and single garlic with concentration of 39.50%, 62.40% and 98.60%. The negative control used NaCl physiology (0.9 %). The result showed that all the treatment group can shortened the time of death of A. galli compared to control. The time of death of A. galli on local garlic infusion with 39.50 % concentration was 6.24 hours, on 62.40 % concentration was 4.94 hours and on 98.60 % concentration was 3.97 hours, kating garlic infusion on 39.50 % concentration was 5.08 hours on 62.40 % concentration was 3.81 hours, on 98.60 % concentration was 2.96 hours, single garlic infusion on 39.50 % concentration was 5.4 hours, on 62.40 % concentrationÂ was 3.86 hours, on 98.60 % concentration was 3.83 hours. The best treatment was kating garlic on 98.60% concentration because give shortest time of death.

The Distribution of Goat Gastrointestinal Tractus Worm Egg at Rambon District of Nganjuk Regency Arum Puspitasari; Boedi Setiawan; Setiawan Koesdarto; Kusnoto Kusnoto; Soeharsono Soeharsono; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the distribution of worm tract infection in foat digestion in Sub-district of Prambon, Nganjuk Regency. This research was conducted in Agustus 2018-Januari 2019 with 108 samples of stool examination iin laboratory of Helmintologi Airlangga University Department of Parasitology, were examinated by native, sedimentation. Floatation techniques, and distribution of worm types of feces. The results showed that 66 samples were positive infected by gastrointestinal worm, its indicated that helminthiasis was 61,11%. On examination it was found some kind of worm eggs, which are: Oesophagustomum spp., Bunostomum spp., Haemonchus spp., Trichostrongylus spp., Strongyloides spp., Trichuris spp., Chabertia spp and Moniezia benedini. The results of study showed the most of worm distribution in feces is Haemonchus spp.

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Jawa timur

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Editorial Address Fakultas Kedokteran Hewan, Kampus “C” Universitas Airlangga. Jl. Mulyorejo Surabaya 60115

Location Kota surabaya, Jawa timur INDONESIA

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Contact Name
Kusnoto

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jps@fkh.unair.ac.id

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+6231-5992785

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jps@fkh.unair.ac.id

Editorial Address
Fakultas Kedokteran Hewan, Kampus “C” Universitas Airlangga. Jl. Mulyorejo Surabaya 60115

Location
Kota surabaya,

Jawa timur

INDONESIA