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Prof. Dr. Imam Mustofa, drh., M.Kes
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Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlaangga, Kampus C Mulyorejo – 60115, Surabaya 3015
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Ovozoa: Journal of Animal Reproduction
Published by Universitas Airlangga
ISSN : 23026464     EISSN : 2722967X     DOI : http://dx.doi.org/10.20473/ovz.v9i1.2020.1-6
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
Ovozoa Journal of Animal Reproduction (Ovozoa J Anim Reprod) published original articles, review articles, and case reports in Indonesian or English, in the scope of veterinary reproduction and animal reproductive biotechnology from researchers, lecturers, students, and practitioners around Indonesia and worldwide. The main objective of the Ovozoa J Anim Reprod is to disseminate the results of scientific researches in the field of animal reproduction. Ovozoa J Anim Reprod invites manuscripts in the areas:  Development of reproductive diagnostic techniques  Development of reproductive technologies  Infectious and non-infectious reproductive diseases  Comparative pathology of reproductive diseases  Reproductive endocrinology  Infertility  Environmental and stress effects on reproduction  Better understanding of the in vivo fertilization process  Better understanding of embryonic development  Artificial insemination and embryo transfer  In vitro fertilisation and embryo culture  Sexual selection of sperm and embryo  Semen and embryo preservation  Reproductive cloning  Stem cell in reproduction  Development of assisted reproductive technologies  Molecular biology, genetics, proteomics, metabolomics application to reproductive biology
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 7 Documents
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Search results for , issue "Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction" : 7 Documents clear
Effect of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic cells and Leydig cells of gentamicin-induced rats Ngizzah, Nailul; Wurlina Wurlina; Poedji Hastutiek; Iwan Sahrial Hamid; Eka Pramyrtha Hestianah; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.99-106

Abstract

This study aimed to determine the effect of the administration of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic and Leydig cells of gentamicin-induced rats (Rattus norvegicus). This study used 25 white male rats divided randomly into five groups. The rats were injected with Gentamicin 5 mg/kg bw/day subcutaneously for 14 days, except for the negative control group (NCG). During the next 14 days, the rats were given oral moringa leaf extract of 200, 316, and 500 mg/kg bw/day respectively for E200, E316, and E500 groups. The data were analyzed with one-way ANOVA followed by Duncan's test. The results showed that the number of spermatogonia, spermatocytes, and spermatids in NCG was higher (p <0.05) than in the other groups. The number of spermatogonia, spermatocytes, and spermatids in the E316 group was higher (p <0.05) than in PCG. The number of spermatogonia in the E500 group was higher (p <0.05) than in the E316 group, but the number of spermatocytes and spermatids in the E316 group was similar (p >0.05) to the E500 group. The number of PCG Leydig cells was the smallest (p <0.05) compared to the other groups. The administration of Moringa leaves extract in the E200 and E316 groups increased (p <0.05) the number of Leydig cells compared to PCG. The number of Leydig PCG cells in the E500 group was not significantly different (p >0.05) from that of the NCG. It could be concluded that administration of Moringa oleifera leaves extract restored the number of spermatogenic and Leydig cells of gentamicin-induced rats.
The addition of egg yolk to the physiological saline extender improved the motility and viability of kampung rooster spermatozoa at cool temperatures Diba, Lucky; Suzanita Utama; Tjuk Imam Restiadi; Suherni Susilowati; Nusdianto Triakoso; Dwi Wijayanti
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.81-89

Abstract

The purpose of this study was to determine the motility and viability of kampung rooster (Gallus gallus domesticus) spermatozoa in 0.9% Sodium chloride (NaCl) solution as an extender with the addition of egg yolk at different concentrations at cool temperature (5°C). This study was performed using two 1.5 years old healthy local roosters. Semen was collected through massage in the morning. The pooled semen sample was divided into four treatment groups. In the T0 group the semen was diluted in 0.9% NaCl, while in the T1, T2 and T3 groups the semen was diluted in 0.9% NaCl added with 5, 10 and 15% egg yolk. The results showed that the motility and viability of spermatozoa decreased when stored at cool temperatures for ten hours (p <0.05). Semen of roosters stored at 5°C in saline solution without the addition of egg yolks showed the lowest motility and viability of spermatozoa (p <0.05). The addition of egg yolk into the saline extender increased the motility and viability of spermatozoa. Concentration of 15% egg yolk in saline solution resulted in the highest spermatozoa motility and viability when stored for up to 8 hours (p <0.05). However, motility and viability of spermatozoa at 10 hours of storage were not significantly different (p >0.05) with the addition of 10% and 15% egg yolks. Therefore, it could be concluded that the addition of 15% egg yolk into a saline solution as an extender could maintain the motility and viability of kampung rooster spermatozoa when stored at 5°C for 10 hours.
Total protein, albumin, and globulin levels of blood serum in repeat breeder Holstein Friesian cows Asrar, Siti Iqmallisa Nurrill; Rimayanti Rimayanti; Ismudiono Ismudiono; Maslichah Mafruchati; Gandul Atik Yuliani; Ginta Riady
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.90-98

Abstract

This study aimed to determine the relationship between total protein, albumin, and globulin in the blood with the incidence of repeat breeders in dairy cows. Ten normal cyclic cows and ten repeat breeders cows were taken randomly based on the reproductive records of the Tani Wilis Village Cooperative, Sendang District, Tulungagung Regency. Questionnaire data in the form of farmers' knowledge about dairy cows' reproductive systems and feed treatment is displayed descriptively.  Dairy cows blood samples were taken from the jugular vein 6 mL to measure total protein, albumin, and globulin levels. Data analyzed using the Statistical Product and Service Solution software at a significance level of 5%. The results showed that the total protein, albumin, and globulin levels in normal dairy cows and dairy cows with repeat breeders were not significantly different (p>0.05). However, in normal cyclic cows, most of the total protein, albumin, and globulin levels (80-100% of normal cyclic cows samples) are within the normal range. Meanwhile, the majority of repeat breeder cows (60-70% of the repeat breeder cows) had total protein, albumin, and globulin levels lower than the normal range (p<0.05). It could be concluded that there was a relationship between the incidence of repeat breeder and total protein, albumin, and globulin levels in the blood serum of dairy cows with repeat breeder. Therefore, it is suggested to raise awareness of smallholder farmers about proper feeding and feeding management to reduce repeat breeder cases.
Spermiogramic parameters of Japanese quails (Coturnix coturnix japonica) to aqueous administration of egg lime molasses mixture Akintunde, Adeyinka Oye; Ndubuisi-Ogbonna, Lois Chidinma; Ladele, Mofiyinfoluwa M.; Olorunfemi, Oladapo Ayodeji; Ojo, Olayinka Abosede; Oyewumi, Samson O.; Bolatito Adenike Shobo; Olufunso Emmanuel Akinboye
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.59-70

Abstract

As demand for animal protein rises, raising Japanese quail holds much potentials in bridging the gap. Phytogenics has been reported to have effect on spermiogramic parameters of animals. This research was done to ascertain the spermiogramic parameters of Japanese quails to administration of aqueous solution of egg lime molasses mixture (ELM). Fresh eggs were placed in a dish, followed by 500g of molasses and 1 liter of lime juice. It was then covered for 10 days and the entire mixture was blended. Two hundred day old Japanese quails were assigned to five treatments in a Completely Randomized Design and were subdivided into 4 replicates of 10 birds each. The control (T1) having no administration of ELM, T2 had an inclusion level of 10 mL, T3: 20 mL, T4: 30 mL and T5: 40 mL, all in 500 mL of water.  Feed and water were provided ad libitum. The study was carried out for 49 days. Data were collected on genitalia morphometry and fertilizing potentials. The birds administered 20 mL of ELM had significantly (p <0.05) higher left epididymis weight (0.36 ± 0.05g) compared to the other groups. The inclusion of ELM in the water significantly influenced (p <0.05) left epididymis volume, paired epididymis volume, left epididymis density, paired epididymis density and spermatozoa reserves in the right epididymis. Testosterone values significantly increased (p <0.05) with increased ELM inclusion. It can be concluded that the administration of ELM did not alter growth parameters however birds that received 20 mL per 500 mL of water had the best reproductive parameters.
Retained placenta in dairy cows living in an all-day cowshed rearing system Sayi Datur Rohmah; Hermin Ratnani; Sunaryo Hadi Warsito; Rimayanti Rimayanti; Sri Pantja Madyawati; Sri Mulyati; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.71-80

Abstract

This study aimed to determine the prevalence and factors that influence the incidence of retained placenta in dairy cows reared under an all-day cowshed housing system at KUD Tani Wilis, Sendang District, Tulungagung. This study used 216 dairy cows, that had already calved, that had been identified to have medical and reproductive records for data exploration. Data collected included the sex and weight of calf, single or twin calves, age of the dam, cowshed structure (floor, puddles on the floor, gutter, distance of cowshed to waste disposal, type of floor and roof, and sunlight exposure), feed of the dam (forage, concentrate, mineral supplements), frequency of retained placenta cases on calving intervals and days open of the dams. The prevalence of retained placenta was presented descriptively. Factors related to the retained placenta and its effect on calving interval and days open were analyzed by Chi-square test using Statistical Product and Service Solution (SPSS) software version 20 for Windows. The result showed that the prevalence rate of retained placenta was 19.91% of all dairy cow populations in the district. Furthermore, the sex of the calf, age of the dam, sunlight exposure, and the quantity of feed (forage and concentrate) were related to the retained placenta (p  <0.05). In addition, retained placenta increased along with extending of days open and calving interval. It could be concluded that the factors that influenced the prevalence of retained placenta were the sex of the calf, the age of the cow, the feed and concentrate, and the cowshed that is not facing the sun.
Handling of endometritis in dairy cow after infection with foot and mouth disease and abortion Amalia Nadila Faradillah; Galuh Chandra Agustina
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.107-113

Abstract

This study aims to report the handling of endometritis in a dairy cow at Koperasi Agro Niaga Jabung, Malang, Indonesia. A Holstein Friesian cross heifer was diagnosed at 258 days pregnant and clinically suffering from Foot and Mouth Disease. On the 266th day of pregnancy, the fetus died, an abortion was diagnosed, and the delivery was assisted by the veterinarian. Furthermore, the cow was treated with 15 mL oxytetracycline intramuscularly. The next day, the veterinarian treated the retained placenta. On the third day after the abortion, the farmer reported that the cow had vaginal discharge which was cloudy white, reddish and smelled bad. The veterinarian treated the cow with an injection of 15 mL of Oxytetracycline. On the sixth day after the abortion, the farmer reported that vaginal discharge still coming out again. Clinical symptoms were, thick, purulent, cloudy, pink discharge from the vagina that hanged in the vulva area and smelled bad. Physical examination showed a body temperature of 40°C, and rectal palpation revealed the uterine wall to be thick, hard, stiff, and getting warmer. Furthermore, the cow was given intrauterine flushing with 1% povidone-iodine (diluted in distilled water), followed by intrauterine administration of 15 mL Oxytetracycline, analgesics, antipyretics, and antispasmodics. Fifteen mL of vitamin B12, ATP, and minerals (Magnesium, Potassium, Sodium selenite) was injected intramuscularly.
Pyometra and mammary gland tumor in a Beagle dog at the Veterinary Teaching Hospital of Brawijaya University, Malang, Indonesia Nofan Rickyawan; Dian Vidiastuti; Karlina Karlina; Shelly Kusumarini
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.114-123

Abstract

A 12-year-old Beagle dog weighing 7.7 kg was brought to the Brawijaya University Veterinary Teaching Hospital in a state of dehydration, lethargy, and jaundice. On the 2nd day of hospitalization, the dog had mucopurulent discharge from the vagina. From the results of a complete blood count and ultrasonography (USG), the patient was diagnosed with pyometra and was treated with an ovariohysterectomy. After surgery the dogs was given cefotaxime 10 mg/kg bw, meloxicam 0.2 mg/kg bw, 0.9 ml vitamin K, and supportive therapy with Biodin® 0.5 mL and Modivitasan® 0.5 mL. Pyometra infection causes progesterone levels to rise, thereby triggering mammary gland tumor. On the 23rd day of hospitalization, a lump was found in the mammary gland. Fine needle aspiration (FNA) results showed the presence of Mott cells. The mammary gland tumor was treated with a single mastectomy. Mastectomy was performed when the dog was in stable condition, about a month after the ovariohysterectomy. Ovariohysterectomy and mastectomy were not performed simultaneously due to consideration of the patient's clinical condition, which made it impossible to get prolonged exposure to anesthesia. Post-mastectomy surgery the dogs were given cefixime 10 mg/kg bw, the analgesic meloxicam 0.2 mg/kg BW, and Biodin® 0.5 ml as supportive therapy. The postoperative wound healed well without complications. The patient recovered after one month of treatment.

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