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All Journal Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary

Hermin Ratnani

Department Of Veterinary Medicine, Faculty Of Veterinary Medicine, Airlangga University, Surabaya

Author-ID : 3488516

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Hambatan Ekspresi Vascular Endothelial Growth Factor oleh Ekstrak Daun Sambung Nyawa pada Endotel Membran Korioalantois (EFFECTIVITY OF SAMBUNG NYAWA LEAF EXTRACT TO INHIBIT VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION ON ENDOTHELIALS OF CHORIOALLANTOIC Iwan Sahrial Hamid; Dady Soegianto Nazar; Hermin Ratnani
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.013 KB)

Angiogenesis is the new blood vessels formation normality and important on growth and developmentof individu. Angiogenesis also have contribution to carcinogenesis or uncontrolled and malignant cancercell development, become pathologic condition like inflammatory and infection. The purpose of this researchfor knew the effectivities of Gynura procumbens extract on various dose for inhibition Vascular EndothelialGrowth Factor (VEGF) expression. This research was done to effort cancer progress inhibition. However,angiogenesis is part of carcinogenesis causes. The Chorio Allantoic Membrane (CAM) methods was usedfor this aim. Eggs at the age of nine days were divided into 6 groups. Group I were negative control ofvehicle, group II were zero treatment: 60 ng bFGF which aplicated into paper dish. The next four groupswere extract of Gynura procumbens that divided in four dose: 60, 75, 90 and 110 µg + bFGF 60 ng whichapplicated into paper dish. At the twelve days old, VEGF expression analysis was done whichimunohystochemical method with anti VEGF’s antibody. The result of this research showed that therewas significant different (p<0.05) on give of Gynura procumbens extract to VEGF expression. The mostsignificant VEGF inhibition by Gynura procumbens extract with dose 110 µg. The conclusion on this studywas Gynura procumbens extract effective to inhibit the VEGF expression on CAM embrio chick.

PENANGANAN GANGGUAN REPRODUKSI UNTUK MENINGKATKAN EFISIENSI REPRODUKSI PADA SAPI PERAH MENUJU SWASEMBADA SUSU DI KECAMATAN SENDANG KABUPATEN TULUNGAGUNG Hermin Ratnani; Dewa Ketut Meles; Imam Mustofa
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 1 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i1.2020.43-52

The aims of local public community partnership program (PKM) with Sumber Makmur Group 3 conducted in Sendang was to :1). Improve the capability of each farmer on treating the cattle 2). Reduce the risk of reproductive disorders which has impact on the insemination 3). Increase the calf-born and milk yield 4). Efficiency in dairy cattle management and 5). Efficiency in feed cost followed by the improved production and reproduction rate in the dairy cattle management. The method used in this partnership program was through training which offer a guidance to farmer the basic information about reproductive disorder also their prevention necessity, and Treatments to the target cattle and also a supplementation of multivitamin to reduce the risk of any reproductive disorders. The program was conduted by 3 veterinary medicine staff from Airlangga university assisted by 2 local people and PPDH-PKL students. The 3 staff of veterinary medicine picked were an expert in reproduction, artificial insemination, and health department as well as an expert in housing and sanitary in dairy cattle management. The program was evaluated by the effectiveness of the training program by using a pre and post test for each individual farmer that was participated. The administered treatments and the reproductive efficiency were also recorded as a comparison before and after the program to show whether it has significant positive or negative result. The evaluation shown a result of each farmer with average pre-test score 58,24% to 77,06% on post-test (32,31%). The reproductive disorders case was reported to have been reduced from 95 to 18 (81,05%). The reproductive efficiency also shown a positive result whereas the Service per conception (S/C) before the program was reduced from > 2-3 times to less than 2 times after the program followed with the service period from 2-3 month into 2 months. The Calving Rate (CR) was also reported to have increased from <50% to >50% after the program.AbstrakTujuan dari program kemitraan masyarakat publik lokal (PKM) dengan Sumber Makmur Group 3 yang dilakukan di Sendang adalah untuk: 1). Tingkatkan kemampuan masing-masing petani dalam merawat ternak 2). Mengurangi risiko gangguan reproduksi yang berdampak pada inseminasi 3). Meningkatkan kelahiran anak sapi dan produksi susu 4). Efisiensi dalam pengelolaan sapi perah dan 5). Efisiensi biaya pakan diikuti oleh peningkatan produksi dan tingkat reproduksi dalam manajemen sapi perah. Metode yang digunakan dalam program kemitraan ini adalah melalui pelatihan yang menawarkan panduan kepada petani informasi dasar tentang gangguan reproduksi serta kebutuhan pencegahan mereka, dan Perawatan untuk ternak target dan juga suplemen multivitamin untuk mengurangi risiko gangguan reproduksi. Program ini disetujui oleh 3 staf kedokteran hewan dari universitas Airlangga dibantu oleh 2 orang lokal dan mahasiswa PPDH-PKL. Tiga staf kedokteran hewan yang dipilih adalah seorang ahli dalam bidang reproduksi, inseminasi buatan, dan departemen kesehatan serta seorang ahli dalam bidang perumahan dan sanitasi dalam manajemen sapi perah. Program ini dievaluasi dengan efektivitas program pelatihan dengan menggunakan tes sebelum dan sesudah untuk masing-masing petani yang berpartisipasi. Perawatan yang diberikan dan efisiensi reproduksi juga dicatat sebagai perbandingan sebelum dan sesudah program untuk menunjukkan apakah itu memiliki hasil positif atau negatif yang signifikan. Evaluasi menunjukkan hasil dari masing-masing petani dengan skor pre-test rata-rata 58,24% sampai 77,06% pada post-test (32,31%). Kasus gangguan reproduksi dilaporkan telah berkurang dari 95 menjadi 18 (81,05%). Efisiensi reproduksi juga menunjukkan hasil positif dimana Layanan per konsepsi (S / C) sebelum program berkurang dari> 2-3 kali menjadi kurang dari 2 kali setelah program diikuti dengan periode layanan dari 2-3 bulan menjadi 2 bulan. Tingkat Calving (CR) juga dilaporkan telah meningkat dari <50% menjadi> 50% setelah program.

PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Itrah Khoirunnisa; Suherni Susilowati; Lilik Maslachah; Tri Wahyu Suprayogi; Rochmah Kurnijasanti; Hermin Ratnani
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.

HUBUNGAN MORFOMETRI AMBING TERHADAP PRODUKSI SUSU KUDA DI DAERAH BIMA NUSA TENGGARA BARAT Mujahid Mujahid; Imam Mustofa; Benjamin Chr Tehupuring; Tjuk Imam Restiadi; Hana Eliyani; Hermin Ratnani
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Objective from this research was to determine the relationship of udder morphometry which consists of the height of the front udder, the height of the rear udder and the circumference ofudder on milk production in the Bima District of West Nusa Tenggara. One of ways to choose a good lactational mare is to pay attention at the shape of the part external body which is udder. The sample had used 32 mares that were in lactation period. Milk production has been measured from the average milk production per day in the lactation period. Milking process in Bima District, West Nusa Tenggara has done in three to four times a day in the span of four to five hours per day. Body length had been measured from os thoracalis to os coxae, chest circumference had been measured from os vertebrae thoracalis to os thoracalis, posterior abdomen of pelvic to os pelvis and the last of os lumbar vetebrae. The research design in this study was survey on every wild mares rancher. Data that has been collected and processed using Multiple Regression with SPSS program version 23th. The result showed that there was a strong relationship between height of rear udder and udder circumference to milk production with back udder with height coefficient of 0.21 (P <0.05) and udder circumference was 0.11 (P <0.05) and equation in Y = 2.423 - 0.398LA + 0.793TAB. However, there is no significant relationship between the height of the front udder and milk production.

Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling Sarah Azura; Hermin Ratnani; Suherni Susilowati; Mas'ud Hariadi; Abdul Samik; Koesnoto Soepranianondo
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.

Penggunaan kombinasi Gonadotropin untuk pengobatan hipofungsi ovarium pada sapi perah Novia Alfi Masruro; Sri Mulyati; Sri Pantja Madyawati; Abdul Samik; Hermin Ratnani; Nenny Harijani
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This case report aims to determine the response of estrus and pregnancy rates in dairy cows with ovarian hypofunction by administering a combination of gonadotropin hormones. The sample used was ten dairy cows that had been calving, were not pregnant, showed no signs of estrus for more than 60 days after the last calving and had ovarian hypofunction based on rectal palpation. All dairy cows were injected 300 IU PG-600, then divided into two groups, each injected with 100 and 300 IU hCG respectively for first group and second group, intramuscularly at the time of insemination. The results showed that the estrus response occurred in all cows, in 6-8 (6.8 ± 0.84) days (first group), and 6-7 (6.2 ± 0.45) days (second group). Trans-rectal palpation pregnancy diagnosis 60 days after artificial insemination showed that all dairy cows in both groups were pregnant. It could be concluded that the treatment of dairy cows suffering from ovarian hypofunction with a combination of 300 IU PG-600 and 100 IU hCG was sufficient to induce 100% estrus rates and 100% pregnancy rates.

Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Kautsar Pandu Pramudita; Widya Paramita Lokapirnasari; Suheni Susilowati; Hermin Ratnani; Erma Safitri; Epy Muhammad Luqman
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.

The effect of watermelon (citrullus lanatus) rind ethanolic extract on the number of leydig, sertoli, and spermatogenic cells of rat (rattus novergicus) exposed to heat Abrian Panggalih Indra Pratama; Suherni Susilowati; Lilik Maslachah; Hermin Ratnani; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.7-11

High temperatures lead to oxidative stress, which can disturb spermatogenesis process. Watermelon (Citrullus lanatus) peel contain antioxidant expected to compensate oxidative stress due to heat stress exposure. This study aimed to determine the effect of watermelon rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of rats exposed to heat (40°C). Twenty rats (Rattus norvegicus) were divided randomly into five groups. In the control group (T0) rats were not exposed to heat nor given the watermelon rind extract. T1, T2, T3, and T4 groups were exposed to heat for an hour daily and orally given placebo (1% Na CMC), 100, 200, and 400 mg/kg BW of watermelon rind extract (in 1% Na CMC). Rats were treated for 52 days, and sacrificed for the testicle collection. Hematoxylin-eosin stained histological slides were prepared for the examination of Leydig, Sertoli and spermatogenic cells. The results showed no significant difference (p >0.05) in the average number of Leydig cells in rats among groups. The number of Sertoli cells and spermatogenic cells of rats exposed to heat (T1) was lower than those of the normal rats (T0 group). The dose of watermelon rind ethanolic extract at 200 mg/kg BW (T3 group) and 400 mg/kg BW (T4) increased (p <0.05) the number of Sertoli and spermatogenic cells. It could be concluded that a dose of 400mg/kg BW of watermelon rind ethanolic extract maintained the number of Leydig cells, Sertoli cells, and spermatogenic cells of rats exposed to heat.

The addition of vitamin C in tris–egg yolk extender maintained Sapera goat semen quality in 5° C storage Jolly Rifqi Pahlevy; Hermin Ratnani; Tjuk Imam Restiadi; Faisal Fikri; Amung Logam Saputro; Bodhi Agustono
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.1-8

Goats are small ruminants that are reared by the rural community for financial income and nutrition. This study was aimed to determine the effect of vitamin C addition in tris–egg yolk extender on the lifespan of Sapera goat spermatozoa stored in 5o C. Semen was collected from 1.5 years old Sapera male goat. The ejaculates were diluted in Tris-egg yolk (T-EY) extender added with 0, 0.2, 0.3, and 0.4 g vitamin C/mL extender for T0, T1, T2, and T3 groups, respectively. The spermatozoa motility, viability, and morphological abnormality were assessed along with storage at 5° C. The result showed that spermatozoa motility was the highest (p <0.05) in T1 compared to other groups and qualified for AI use for up to 72 hours. In 24 hours storage, the spermatozoa viability was not significantly different (p >0.05) among the groups. The spermatozoa viability in the T1 group was the highest (p <0.05) among the groups along 48-96 hours of storage. The spermatozoa morphological abnormalities of the T1 group was the lowest (p <0.05) compared to other treatment groups in the range of 24-72 hour storage. In the control group (T0), the less than 5% spermatozoa morphological abnormalities (qualified for artificial insemination) were only in the 24 hours storage, while those of the T1 group were up to 72 hours. It could be concluded that the addition of 0.2 g vitamin C/100 mL T-EY extender maintained the quality of Sapera goat semen for 72 hours at 5°C.

Retained placenta in dairy cows living in an all-day cowshed rearing system Sayi Datur Rohmah; Hermin Ratnani; Sunaryo Hadi Warsito; Rimayanti Rimayanti; Sri Pantja Madyawati; Sri Mulyati; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.71-80

This study aimed to determine the prevalence and factors that influence the incidence of retained placenta in dairy cows reared under an all-day cowshed housing system at KUD Tani Wilis, Sendang District, Tulungagung. This study used 216 dairy cows, that had already calved, that had been identified to have medical and reproductive records for data exploration. Data collected included the sex and weight of calf, single or twin calves, age of the dam, cowshed structure (floor, puddles on the floor, gutter, distance of cowshed to waste disposal, type of floor and roof, and sunlight exposure), feed of the dam (forage, concentrate, mineral supplements), frequency of retained placenta cases on calving intervals and days open of the dams. The prevalence of retained placenta was presented descriptively. Factors related to the retained placenta and its effect on calving interval and days open were analyzed by Chi-square test using Statistical Product and Service Solution (SPSS) software version 20 for Windows. The result showed that the prevalence rate of retained placenta was 19.91% of all dairy cow populations in the district. Furthermore, the sex of the calf, age of the dam, sunlight exposure, and the quantity of feed (forage and concentrate) were related to the retained placenta (p <0.05). In addition, retained placenta increased along with extending of days open and calving interval. It could be concluded that the factors that influenced the prevalence of retained placenta were the sex of the calf, the age of the cow, the feed and concentrate, and the cowshed that is not facing the sun.

Co-Authors AA Sudharmawan, AA Abdul Samik Abdullah Hasib Abrian Panggalih Indra Pratama Agustono, Bodhi Benjamin Chr Tehupuring Berliana, Erprinanda Galuh BUDI UTOMO Budi Utomo Budiarto Budiarto Cindy Ercha Aulia Putri Dady Soegianto Nazar Dinul, Ahmad Rajul Epy Muhammad Luqman ERMA SAFITRI Faisal Fikri Frida Aulya Arningdiah Hana Eliyani Hani Plumeriastuti Hanifah, Novi Fitria Imam Mustofa Imam Mustofa Itrah Khoirunnisa Iwan Sahrial Hamid Jolly Rifqi Pahlevy Kadek Rachmawati Kautsar Pandu Pramudita Keren Jayanti Sofie Adi Koesnoto Soepranianondo Lilik Maslachah Lilik Maslachah Luqman, Epy Muhammad Mafruchati, Maslichah Mas'ud Hariadi Meles, Dewa Ketut Mufasirin Muhammad Al-Syafiq bin Abdul Halim Mujahid Mujahid Nenny Harijani Novia Alfi Masruro Nurhusien Yimer Nuurin Ajrin Karim Prastiya, Ragil Angga Pudji Srianto Rahmatullah, Aldin Akbar Ramadhani, Fifi Fauziah Restiadi, Tjuk Imam Restiadi, Tjuk Imam Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Saputro, Amung Logam Sarah Azura Sayi Datur Rohmah Setiawan Koesdarto Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati Suheni Susilowati Suherni Susilowati Suherni Susilowati Sunaryo Hadi Warsito Suzanita Utama Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yulianna Puspitasari Zahrorona, Amira Halimah

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