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All Journal Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary
Hermin Ratnani
Department Of Veterinary Medicine, Faculty Of Veterinary Medicine, Airlangga University, Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Hambatan Ekspresi Vascular Endothelial Growth Factor oleh Ekstrak Daun Sambung Nyawa pada Endotel Membran Korioalantois (EFFECTIVITY OF SAMBUNG NYAWA LEAF EXTRACT TO INHIBIT VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION ON ENDOTHELIALS OF CHORIOALLANTOIC Iwan Sahrial Hamid; Dady Soegianto Nazar; Hermin Ratnani
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.013 KB)

Abstract

Angiogenesis is the new blood vessels formation normality and important on growth and developmentof individu. Angiogenesis also have contribution to carcinogenesis or uncontrolled and malignant cancercell development, become pathologic condition like inflammatory and infection. The purpose of this researchfor knew the effectivities of Gynura procumbens extract on various dose for inhibition Vascular EndothelialGrowth Factor (VEGF) expression. This research was done to effort cancer progress inhibition. However,angiogenesis is part of carcinogenesis causes. The Chorio Allantoic Membrane (CAM) methods was usedfor this aim. Eggs at the age of nine days were divided into 6 groups. Group I were negative control ofvehicle, group II were zero treatment: 60 ng bFGF which aplicated into paper dish. The next four groupswere extract of Gynura procumbens that divided in four dose: 60, 75, 90 and 110 µg + bFGF 60 ng whichapplicated into  paper dish.  At the twelve days old, VEGF expression analysis was done whichimunohystochemical method with anti VEGF’s antibody. The result of this research showed that therewas significant different (p<0.05) on give of Gynura procumbens extract to VEGF expression. The mostsignificant VEGF inhibition by Gynura procumbens extract with dose 110 µg. The conclusion on this studywas Gynura procumbens extract effective to inhibit the VEGF expression on CAM embrio chick.
PENANGANAN GANGGUAN REPRODUKSI UNTUK MENINGKATKAN EFISIENSI REPRODUKSI PADA SAPI PERAH MENUJU SWASEMBADA SUSU DI KECAMATAN SENDANG KABUPATEN TULUNGAGUNG Hermin Ratnani; Dewa Ketut Meles; Imam Mustofa
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 1 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i1.2020.43-52

Abstract

The aims of local public community partnership program (PKM) with Sumber Makmur Group 3 conducted in Sendang was to :1). Improve the capability of each farmer on treating the cattle 2). Reduce the risk of reproductive disorders which has impact on the insemination 3). Increase the calf-born and milk yield 4). Efficiency in dairy cattle management and 5). Efficiency in feed cost followed by the improved production and reproduction rate in the dairy cattle management. The method used in this partnership program was through training which offer a guidance to farmer the basic information about reproductive disorder also their prevention necessity, and Treatments to the target cattle and also a supplementation of multivitamin to reduce the risk of any reproductive disorders. The program was conduted by 3 veterinary medicine staff from Airlangga university assisted by 2 local people and PPDH-PKL students. The 3 staff of veterinary medicine picked were an expert in reproduction, artificial insemination, and health department as well as an expert in housing and sanitary in dairy cattle management. The program was evaluated by the effectiveness of the training program by using a pre and post test for each individual farmer that was participated. The administered treatments and the reproductive efficiency were also recorded as a comparison before and after the program to show whether it has significant positive or negative result. The evaluation shown a result of each farmer with average pre-test score 58,24% to 77,06% on post-test (32,31%). The reproductive disorders case was reported to have been reduced from 95 to 18 (81,05%). The reproductive efficiency also shown a positive result whereas the Service per conception (S/C) before the program was reduced from > 2-3 times to less than 2 times after the program followed with the service period from 2-3 month into 2 months. The Calving Rate (CR) was also reported to have increased from <50% to >50% after the program.AbstrakTujuan dari program kemitraan masyarakat publik lokal (PKM) dengan Sumber Makmur Group 3 yang dilakukan di Sendang adalah untuk: 1). Tingkatkan kemampuan masing-masing petani dalam merawat ternak 2). Mengurangi risiko gangguan reproduksi yang berdampak pada inseminasi 3). Meningkatkan kelahiran anak sapi dan produksi susu 4). Efisiensi dalam pengelolaan sapi perah dan 5). Efisiensi biaya pakan diikuti oleh peningkatan produksi dan tingkat reproduksi dalam manajemen sapi perah. Metode yang digunakan dalam program kemitraan ini adalah melalui pelatihan yang menawarkan panduan kepada petani informasi dasar tentang gangguan reproduksi serta kebutuhan pencegahan mereka, dan Perawatan untuk ternak target dan juga suplemen multivitamin untuk mengurangi risiko gangguan reproduksi. Program ini disetujui oleh 3 staf kedokteran hewan dari universitas Airlangga dibantu oleh 2 orang lokal dan mahasiswa PPDH-PKL. Tiga staf kedokteran hewan yang dipilih adalah seorang ahli dalam bidang reproduksi, inseminasi buatan, dan departemen kesehatan serta seorang ahli dalam bidang perumahan dan sanitasi dalam manajemen sapi perah. Program ini dievaluasi dengan efektivitas program pelatihan dengan menggunakan tes sebelum dan sesudah untuk masing-masing petani yang berpartisipasi. Perawatan yang diberikan dan efisiensi reproduksi juga dicatat sebagai perbandingan sebelum dan sesudah program untuk menunjukkan apakah itu memiliki hasil positif atau negatif yang signifikan. Evaluasi menunjukkan hasil dari masing-masing petani dengan skor pre-test rata-rata 58,24% sampai 77,06% pada post-test (32,31%). Kasus gangguan reproduksi dilaporkan telah berkurang dari 95 menjadi 18 (81,05%). Efisiensi reproduksi juga menunjukkan hasil positif dimana Layanan per konsepsi (S / C) sebelum program berkurang dari> 2-3 kali menjadi kurang dari 2 kali setelah program diikuti dengan periode layanan dari 2-3 bulan menjadi 2 bulan. Tingkat Calving (CR) juga dilaporkan telah meningkat dari <50% menjadi> 50% setelah program.
Pengaruh Konsentrasi Gliserol dalam Pengencer Tris Terhadap Kualitas Spermatozoa Kambing Sapera Before Freezing Hanifah, Novi Fitria; Ratnani, Hermin; Restiadi, Tjuk Imam; Agustono, Bodhi; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.154-159

Abstract

Penelitian ini bertujuan untuk menentukan konsentrasi gliserol yang optimal dalam pengencer tris dalam mempertahankan kualitas spermatozoa kambing Sapera. Penelitian ini menggunakan kambing Sapera jantan umur 1.5 tahun. Konsentrasi gliserol yang digunakan adalah 5%, 6% dan 7%. Semen ditampung dua kali dalam seminggu dengan menggunakan vagina buatan. Hasil penelitian menunjukkan rata-rata persentase motilitas dan viabilitas spermatozoa pada pengencer dengan gliserol 6% masing-masing 62.33% dan 75.83% terdapat perbedaan yang signifikan (p<0.05) dibandingkan pada pengencer dengan gliserol 5% masing-masing 57.67% dan 68.33% dan 7% masing-masing 59% dan 70.33%. Rata-rata abnormalitas pada pengencer dengan gliserol 5%, 6% dan 7% masing-masing 8%, 7.5% dan 7.67% tidak terdapat perbedaan yang signifikan (p>0.05). Dapat disimpulkan bahwa penambahan gliserol 6% dalam pengencer Tris berhasil melindungi spermatozoa dari cekaman dingin selama proses gliserolisasi, sehingga dapat mempertahankan motilitas, vibilitas dan abnormalitas spermatozoa.
Service per Conception, Conception Rate, Calving Rate and Non-Return Rate in Beef Cattle in Kalipuro, Banyuwangi Dinul, Ahmad Rajul; Restiadi, Tjuk Imam; Wibawati, Prima Ayu; Ratnani, Hermin; Saputro, Amung Logam; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.54-61

Abstract

This study aimed to determine the number of reproductive efficiency parameters including service per conception, conception rate, calving rate, and non-return rate for broilers in Kalipuro District, Banyuwangi Regency. The secondary data was collected from the Banyuwangi District Agriculture Office. This research is also intended to dissect factors related to the S/C, CR, CvR, and NRR figures with descriptive analysis, including bio reproduction mastery factors, service factors, and feed factors. The primary data was collected from beef cattle breeders in Kalipuro District, Banyuwangi Regency. In addition, there were IB implementation factors whose data were obtained from questionnaires to inseminators in Kalipuro District, Banyuwangi Regency. The number of S/C broilers in Kalipuro Subdistrict, Banyuwangi Regency was good maintenance, in January-March 2020 of 1.71, 1.99, and 1.74, meanwhile, the number of CR were 58%, 50% 57%, CvR for January-March were 44%, 84%, 53% and NRR for January, February and March were 41%, 49%, and 46%, respectively, were below the standard rate.
Profile of Progesterone Levels After Administration of Gonadotropins in Dairy Cattle with Ovarian Hypofunction Berliana, Erprinanda Galuh; Utama, Suzanita; Wurlina, Wurlina; Srianto, Pudji; Lestari, Tita Damayanti; Ratnani, Hermin
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.1-5

Abstract

This study aimed to evaluate the profile of progesterone in dairy cattle with ovarian hypofunction. A total of 10 cows in this study were evaluated three times in the collection phase, i.e. (F1) when the cow was diagnosed with ovarian hypofunction, (F2) when the cow was in heat and (F3) 21 days after artificial insemination to detect pregnancy. Samples in the form of blood serum were then analyzed using ELISA. As a result, the average time of heat in ovarian hypofunction cows was 7,4 days. Progesterone levels in F1 were 1,027 ng/ml, 2,774 ng/ml, 1,476 ng/ml, 2,256 ng/ml, 1,258 ng/ml, 1,758 ng/ml, 2,393 ng/ml, 0,592 ng/ml, 0,755 ng /ml, 1,876 ng/ml, respectively. Progesterone levels in F2 were 0,671 ng/ml, 0,517 ng/ml, 0,763 ng/ml, 0,598 ng/ml, 0,615 ng/ml, 0,537 ng/ml, 0,726 ng/ml, 0,643 ng/ml, 0,593 ng/ml, 0,975ng/ml, respectively. Progesterone levels in F3 were 15,642 ng/ml, 4,215 ng/ml, 17,327 ng/ml, 20,721 ng/ml, 5,796 ng/ml, 17,214 ng/ml, 15,815 ng/ml, 16,745 ng/ml, 4,632 ng /ml, 18,281 ng/ml, respectively. The pregnancy rate in hypofunctional cows treated with PG-600 in this study was 70%.
PENGARUH PEMAPARAN KARBOFURAN PADA INDUK MENCIT (Mus musculus) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL ANAK MENCIT MASA LAKTASI Purnomo, Edi; Luqman, Epy Muhammad; Ratnani, Hermin; Plumeriastuti, Hani; Mafruchati, Maslichah; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21095

Abstract

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.
Mulberry (Morus alba L.) leaf extract enhanced spermatozoa motility, viability, and plasma membrane integrity of rats (Rattus norvegicus) exposed to e-cigarette smoke Ramadhani, Fifi Fauziah; Yulianna Puspitasari; Hermin Ratnani; Budi Utomo; Rochmah Kurnijasanti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.120-128

Abstract

This study investigates the impact of mulberry leaf extract on the viability, plasma membrane integrity, and motility of spermatozoa from male white rats (Rattus norvegicus) exposed to e-cigarette smoke. A total of twenty-five male rats were divided into five groups: negative control (NC), positive control (PC), T1, T2, and T3. All groups, except for the NC group, were exposed to e-cigarette smoke. Rats in the T1, T2, and T3 groups received mulberry leaf extract in doses of 100, 200, and 400 mg/kg bw, respectively, while the NC and PC groups were given a placebo of 1% Na-CMC. Both the mulberry leaf extract and the placebo were administered daily, beginning three days prior to the start of e-cigarette smoke exposure, which lasted for 28 days. Results showed that spermatozoa motility, plasma membrane integrity, and viability in the experimental groups were significantly lower than those in the NC group (p <0.05). Conversely, rats in the T1, T2, and T3 groups that received mulberry leaf extract demonstrated significantly greater spermatozoa viability, plasma membrane integrity, and motility compared to the PC group (p <0.05). The T3 group exhibited the most pronounced improvements, with significantly enhanced spermatozoa viability, membrane integrity, and motility (p <0.05) relative to the PC group. These results indicate that mulberry (Morus alba L.) leaf extract enhanced spermatozoa viability, plasma membrane integrity, and motility in white rats (Rattus norvegicus) subjected to e-cigarette smoke.
Estrus quality, non-return rate and conception rate in the incidence of repeat breeding in dairy cow after 2%povidone iodine therapy combined with prostaglandin F2α at KUD Tani Wilis Tulungagung Zahrorona, Amira Halimah; Sri Pantja Madyawati; Pudji Srianto; Hermin Ratnani; Sunaryo Hadi Warsito; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.46-51

Abstract

Repeat breeding is a condition in which cows fail to conceive after being bred three or more times. This study aims to determine the quality of estrus, non-return rate (NRR) and conception rates obtained after cows were treated with 2% povidone iodine. The study involved 30 Holstein Friesian cows, each having calved three times and experiencing repeat breeding. Cows in estrus were treated with 10 mL of 2% povidone iodine intrauterinely, followed by an intramuscular injection of 2 mL (250 mg/head) of prostaglandin F2α (PGF2α, Luteosyl) five days later. Estrus quality was observed by examining changes in vulvar mucosa color, mucus discharge, and vulvar temperature. Pregnancy diagnosis was made at 60 days. Results showed that 43.3% of cows had estrus quality score 3 (cows showed dark red vulvar mucosa, transparent mucus, temperature 38.9 ± 0.5 ºC), 23.3% had score 2 (cows showed reddish vulvar mucosa, some cloudy mucus, temperature 38.7 ± 1.0 ºC), and 33.3% had score 1 (cows showed pink vulvar mucosa, cloudy mucus, temperature 38.6 ± 0.5 ºC). Of the 30 cows, 20 had a non-return rate (NRR) of 66.7%. Based on these results, it can be concluded that 2% povidone iodine has a positive effect on estrus quality, NRR, and conception rates in dairy cows experiencing repeat breeding.
Prevalence and Risk Factor of Repeat Breeder Syndrome in Dairy Cows in KUD Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province Frida Aulya Arningdiah; Rahmatullah, Aldin Akbar; Cindy Ercha Aulia Putri; Mufasirin; Hermin Ratnani; Rimayanti; Sri Pantja Madyawati; Tita Damayanti Lestari; Muhammad Al-Syafiq bin Abdul Halim; Nuurin Ajrin Karim
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.232-242

Abstract

This study aims to contribute valuable insights to readers and serve as a reference for dairy farmers looking to enhance their operations. By examining the prevalence and risk factors associated with repeat breeder syndrome in dairy cows within the Village Unit Cooperation (KUD) Bebarengan Anggayuh Tentrem Urip, Batu City, East Java Province, this study aims to aid in preventing such occurrences and ultimately improve dairy cow productivity. The survey method was employed, utilizing data sourced from cooperative and farmer records, as well as observations, interviews, and questionnaire responses. Analysis revealed that out of 50 farmers owning a total of 281 cows, 30 farmers possessed a combined total of 58 dairy cows classified as repeat breeders. Consequently, the prevalence rate of repeat breeders among farmers was found to be 20.64%. Descriptive analysis was conducted on the collected data, with additional SPSS Chi-Square (X²) analysis performed to identify risk factors associated with repeat breeders. These risk factors include breeder experience, knowledge of reproduction and estrus phases, communication with artificial inseminators and veterinarians, housing conditions, as well as factors related to livestock feed and water.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Khoirunnisa, Itrah; Susilowati, Suherni; Maslachah, Lilik; Suprayogi, Tri Wahyu; Kurnijasanti, Rochmah; Ratnani, Hermin
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.347 KB) | DOI: 10.20473/ovz.v8i2.2019.127-131

Abstract

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.
Co-Authors AA Sudharmawan, AA Abdul Samik, Abdul Abdullah Hasib Agustono, Bodhi Azura, Sarah Berliana, Erprinanda Galuh BUDI UTOMO Budiarto Budiarto Cindy Ercha Aulia Putri Dady Soegianto Nazar Dinul, Ahmad Rajul Erma Safitri Faisal Fikri Frida Aulya Arningdiah Hana Eliyani Hani Plumeriastuti Hanifah, Novi Fitria Hariadi, Mas'ud Harijani, Nenny Imam Mustofa IMAM MUSTOFA Iwan Sahrial Hamid Kadek Rachmawati Khoirunnisa, Itrah Lilik Maslachah Luqman, Epy Muhammad Mafruchati, Maslichah Masruro, Novia Alfi Meles, Dewa Ketut Mufasirin Muhammad Al-Syafiq bin Abdul Halim Mujahid Mujahid, Mujahid Nurhusien Yimer Nuurin Ajrin Karim Pahlevy, Jolly Rifqi Pramudita, Kautsar Pandu Prastiya, Ragil Angga Pratama, Abrian Panggalih Indra Pudji Srianto Rahmatullah, Aldin Akbar Ramadhani, Fifi Fauziah Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Saputro, Amung Logam Sayi Datur Rohmah Setiawan Koesdarto Soepranianondo, Koesnoto Sofie Adi, Keren Jayanti Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Susilowati, Suheni Suzanita Utama Tehupuring, Benjamin Chr Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yulianna Puspitasari Zahrorona, Amira Halimah