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INDONESIA
Medicra (Journal of Medical Laboratory Science/Technology)
Published by Universitas Muhammadiyah Sidoarjo
ISSN : 25807730     EISSN : 25807730     DOI : https://doi.org/10.21070/medicra
Core Subject : Health,
Health Professions Public Health
Focus : to facilitate scholar, researchers, and lecturers for publishing the original articles of review articles. Scope : Medicra publishes research articles in the field of “medical laboratory (science/technology)” with the following scope: Clinic Chemical Hematology Microbiology Parasitology Immunology Food and beverage analysis Chemical Molecular Diagnostics Toxicology Cytology Histology Epidemiology Laboratory Management Laboratory Quality Control
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 121 Documents
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Penentuan Konsentrasi Ammonium dalam Air Sungai Pelayaran Ngelom: Determination of Ammonium Concentration in Ngelom . Shipping River Water Ngibad, Khoirul
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.2071

Abstract

Ammonium (NH4+) is a form of ammonia (NH) compound in thewater at low pH. The ammonium can cause poisoning in aquatic life. The purpose of this study was to determine the ammonium concentration in the Ngelom river water sample that flows in the Ngelom village, Taman district, Sidoarjo Regency. The measurement of ammonium concentration in river water samples was carried out using the UV-Vis spectrophotometrymethod with Nessler reagent based on the Nessler reagent reaction (K2HgI4) with ammonium in a base solution which would form a colloidal yellow brown dispersion which absorbance measured at 410 nm. Ammonium absorbance is proportional to the ammonium concentration in the sample. The sampling technique in this study used purposive sampling. Samples were taken at 10 trial points, every 5 meters to the right and left from the center point (Ngelom's River bridge to Maarif Hasyim Latif University). Ammonium concentration was determined in the linear concentration range of 1 – 5 mg/L with a correlation coefficiency value (r) of 0.9615 with limit of detection and limit of quantitation of 0.105 mg/L and 0.352 mg/L, respectively. The results of testing the river water samples showed that the ammonium concentration in 10 samples of river water ranged from 1.61 – 14.68 mg/L. All of the sample was not eligible requirements of the Republic of Indonesia PP No. 82 of 2001 about Water Quality Management and Water Pollution Control.
Ekstrak Daun Keji Beling (Strobilanthes crispus L.) Uuntuk Penurunan Kadar Kolesterol Pada Tikus Putih Jantan (Ratus norvegicus) Galur Wistar: Keji Beling (Strobilanthes crispus L.) Leaf Extract for Reducing Cholesterol Levels in Male White Rats (Ratus norvegicus) Wistar strain Rachman, Fadila Abd; Ardiansyah, Syahrul
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1648

Abstract

Indonesian citizens have a various lifestyle impacts on the increasing of blood-cholesterol level. One of the potential herbal medicine is keji beling leaves (Strobilanthes crispus L.) because of these leaves contains flavonoid and sterol. This study aims to analyze the efficacy of Strobilanthes crispus L. leaves extract to decrease the blood-cholesterol level. This research is experimental laboratory with pretest – post test only control group design. The total of 24 Wistar rats were divided into six groups, as follow: negative control, positive control, treated with 25%, 50%, 75% and 100% Strobilanthes crispus L. extract. The therapy was administrated for 14 days. Cholesterol test using POCT method. The data were analyzed using Repeated Measured Anova. The results showed that Strobilanthes crispus L. leaves extracts potentially decreased on blood-cholesterol level in Wistar rats after 14 days of treatment. This study reflected that the Strobilanthes crispus L. leaves extract can be used as the alternative therapy on the blood-cholesterol level. The greatest decrease in P4 (100% concentration).
Aktivitas Antibakteri Kombinasi Ekstrak Daun Mengkudu (Morinda citrifoli L.) dan Bawang Putih (Allium sativum) terhadap Escherichia coli dan Staphylococcus aureus: Antibacterial Activity of Noni Leaf Extract (Morinda citrifoli L.) and Garlic (Allium sativum) against Escherichia coli and Staphylococcus aureus Lestari, Indah; Hanum, Galuh Ratmana
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.1475

Abstract

Indonesia is a nation with rich medicinal plants. Medicinal plants can be used for the treatment of diseases due to bacterial infection. Noni leaf (Morinda citrifolia) and garlic (Allium sativum) are medicinal plants and herbs that have the potential to inhibit or kill Escherichia coli and Staphylococcus aureus. The aim of this research is to know the antibacterial activity on the combination of extract noni leaf (Morinda citrifolia) and garlic (Allium sativum) to growth of Escherichia coli and Staphylococcus aureus. In this study using a research design that is a complete randomized design with purposive judgment sampling method.Anti-bacterial activity test performed by the disk diffusion method. The concentration of extract used is 10%, 30%, 60% and 90%. The analysis was done by Kruskal-Wallis test. The results of this study showed that a combination of 30% leaf extract and 10% garlic extract able to inhibit the growth of Staphylococcus aureus bacteria optimally with the average area of the inhibit zone is 12 mm. While on the combination of 30% noni leaf extract and 90% garlic extract able to inhibit the growth of Eschericia coli optimally with the average area of inhibit zone is 22,27 mm.
Formulasi dan Evaluasi Sabun Cair Ekstrak Etanol Okra (Abelmochus esculentus) serta Uji Antibakterinya: Formulation and Evaluation of Okra (Abelmochus esculentus) Ethanol Extract Liquid Soap and Its Antibacterial Test Martina, Shofie Fitria; Rohmah, Jamilatur
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.1657

Abstract

Liquid soap is a liquid form that serves to cleanse the skin. The aim of this research are to know the optimization formulation and evaluation of liquid soap extract ethanol okra ( ) and also find the best concentration of okra extract as the inhibitors against Staphylococcus . Experimental laboratory was chosen as the research design. Liquid soap consists of 5 formulations, namely formulation 1, 2, 3, 4, and 5 which consist of 0%, 25%, 50%, 75% and 100% okra concentration. The antibacterial effectiveness was tested by disc method against liquid soap extract ethanol okra ( ) and the clear zone measured. The analysis done with two-way test. The results of the evaluation of liquid soap quality was appropriate with the standard of soap quality established by SNI 1994. The best moisture value found in the formulation of 5 (F5) with 0%form of 3.54%, the best free fatty acid found in formulation 4 (F4) with a concentration of 50% of 0.07135%, the best free alkali concentration was found in formulation 2 (F2) with a 25% concentration of 0.04194%, the best pH value was presented at concentrations of 0%, 25%, and 50% with a value of 9, the best value of foam stability found in Formula 1 (F1) with a 100% concentration of 9.1, the best density (BJ) test found in Formula 4 (F4) with 0% concentration. The result of ANOVA test with variation of ethanol okra concentration obtained p-value = 0.000 <0.05 which states there was a significant difference where liquid soap extract ethanol okra has antibacterial to Staphylococcus . The best inhibitory zone test resulted in formation 5 (F5) of 14.7 mm at 100% concentration.
Identifikasi Aspergillus flavus pada Kue Pia yang Di Jual Di Dusun Warurejo Kabupaten Pasuruan: Identification of Aspergillus flavus on Pia Cakes Sold in Warurejo Hamlet, Pasuruan Regency Lindawati, Sari; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.1618

Abstract

Warurejo village that located in Pasuruan district is the center of pia cake business expecially green beans pia. Pia cake is a cake that can’t durable cause by microorganism that come from the less clear and wraping in production. It makes contamination of mycotoxins from spesies of the fungus is Aspergillus flavus. Aspergillus flavus can produce a toxin metabolite compund that is aflatoxin. If aflatoxin is continuous consumtion will cause liver cancer. This research aim to determine presence of Aspergillus flavus on pia cake that produce by center of pia cake business in Warurejo village. It will be research with storage time of 0, 5 and 10 days. This study is descriptive, the number of sample taken randomly as many as 30 samples from 33 stores. Research this is obtained by macroscopically and microscopically. In macroscopically colonis that, look yellowish greeen and on the bottom is yellow to brown while microscopically rounded vesicles and conidi are round. 73% of Aspergillus flavus has positive contaminated fungus in 0 days and 5 days. 97% positive was Aspergillus flavus with 10 days storage time. In day 10 found more Aspergillus flavus fungus that on days 0 and 5 days.
Kinerja Fungsi Ginjal pada Kelinci (Lepus nigricollins) Diabetes yang diberi Ekstrak Bonggol Buah Nanas (Ananas comusus L.): Performance of Kidney Function in Rabbits (Lepus nigricollins) Diabetic given Pineapple (Ananas comusus L.) Weevil Extract Rahmayanti, Very; Ardiansyah, Syahrul
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.1652

Abstract

Bromelain enzyme contained in pineapple (Ananas comosus L.) are enzyme that have a function as anti-inflamatory in diabetic treatment periodically, will be giving impact to kidney function performance. This study to determine the effect of pineapple hump extract (Ananas comosus L.) on creatinine level in diabetic rabbits. Rabbits were acclimated for 3 weeks than measured the creatinine level to know the first level. So, it induced by alloxan as much as 195 mg/kg during ±2 weeks for conditioning under hyperglicemia. Then measured the creatinine level from 6 groups, it given pineapple extract with different concentration, those are positive control (+), negative control (-), extract 25%, 50%, 75% and 100%. Then measured of creatinine level did in enzymatic photometric method. In group of concentration 25% the creatinine level from 0,58 mg/dl become 1,07 mg/dl, in concentration 50% from 1,55 mg/dl become 1,25 mg/dl, in concentration 75% from 1,63 mg/dl become 1,19 mg/dl and in concentration 100% from 1,58 mg/dl become 1,42 mg/dl. The result of the study were tested statiscally with one way Anova test, it showed p> 0,05. It show Pineapple hump extract has no effect on the role of diabetic rabbits kidney.
Pengaruh Ekstrak Daun Kedondong (Spondias dulcis)Terhadap Pertumbuhan Trichophyton rubrum Secara In Vitro: Effect of Kedondong (Spondias dulcis) Leaf Extract on In Vitro Growth of Trichophyton rubrum Nirosa, Givari Dwi; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.1691

Abstract

Dermatophytes are fungi that can cause dermatophytosis, this fungus attacks the skin of the stratum corneum. This study aims to determine the effectiveness Spondias dulcis leaf extract on the growth of Trichophyton rubrum in vitro. This research uses Trichophyton rubrum and Spondias dulcis leaf extract. The treatment in this study was divided into 6 treatment groups, namely negative control, concentration of 20%, 40%, 80%, 100% and positive control, as well as 4 repetitions. After incubation for 5-7 days then observations were made and the results showed that Spondias dulcis leaf extract with various concentrations did not have different results, there was no effect on the growth of Trichophyton rubrum in vitro. Negative control used 10% DMSO solution which showed no effect on the growth of Trichophyton rubrum. On positive control using terbinafine antifungal drugs showed results that were very influential on the growth of Trichophyton rubrum. So it can be concluded that Spondias dulcis leaf extract has no influence on the growth of Trichophyton rubrum.
Hubungan Paparan Pestisida Kadar Enzim Kolinesterase dengan Kadar Trigliserida pada Pekerja yang Terpapar Pestisida Golongan Organofosfat: Relationship of Pesticide Exposure to Cholinesterase Enzyme Levels with Triglyceride Levels in Workers Exposed to Organophosphate Group Pesticides Santoso, Andreas Putro Ragil; Wulandari, Devyana Dyah
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.2992

Abstract

Alzaimer degerative diseases that attack the brain that occur due to several things including cholesterol and poisoning due to organopathic pesticides, cholinesterase. Triglycerides are a part of kolesterol which consists of about 20% of total cholesterol. Pesticide buildup in the body often occurs due to work risks. This study aims to determine how much cholinesterase activity and triglyceride levels are in workers exposed to organophosphate pesticides, and to determine the relationship of pesticide exposure to cholinesterase enzyme levels with triglyceride levels in workers who are exposed to workers exposed to pesticides. This research is an observational research with an experimental approach. The study was conducted at PT.X, which was exposed to 15 pesticides. This study uses a correlation test that will be shown if p <0.05. Based on the results of the study obtained based on the average results of cholinesterase activity obtained at 8,040 U/L while triglycerides at 186 mg/dL. And after the correlation test was obtained at 0.085 indicating that p> 0.05 so it was concluded that there was no relationship between cholinesterase activity with triglyceride levels.
Relationship Between Transaminase Enzyme Levels And Gamma GT In Diabetes Mellitus Patients With Diabetic Foot Ulcers: Hubungan Kadar Enzim Transaminase Dengan Gamma GT Pada Pasien Diabetes Mellitus Dengan Ulkus Kaki Diabetik Emilia, Hilda; Aliviameita, Andika
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1461

Abstract

Diabetes Mellitus is a chronic disease when the body cannot use insulin or cannot produce insulin enough, and glucose level in the blood increases. Diabetic ulcers are open sores on the skin surface caused by complications of macroangiophaty which can lead to neurophaty and vascular insufficiency. This study aims to determine the relationship between transaminase enzyme levels and Gamma GT in diabetes mellitus patients with diabetic foot ulcers. The sample used in this study was 30 patients with diabetes mellitus with diabetic foot ulcers. Research samples were obtained at Rumah Luka Sidoarjo and examined in the Hematology Laboratory of the Faculty of Health Sciences Muhammadiyah University in March 2021. SGOT and SGPT examination used the UV Enzymatic method, while the Gamma GT examination used the Enzymatic colorimetric method. In the Correlation-Regression test, it was found that there was no significant relationship (p=0,481) between SGOT Gamma GT, but there was a significant relationship (p=0,037) between SGPT and Gamma GT. Conclusion there is a moderate correlation (r=0,413) between transaminase anzymes and gamma GT in diabetes mellitus patients with diabetic foot ulcers.
Analysis of Lead (Pb) and Zinc (Zn) Levels in Milkfish (Chanos Chanos) at Kalanganyar Market Sidoarjo Using Atomic Absorption Spectrophotometer (Aas) Method: Uji Kadar Timbal (Pb) dan Seng (Zn) pada Ikan Bandeng (Chanos chanos) di Pasar Kalanganyar Sidoarjo dengan Metode Atomic Absorption Spectrophotometer (AAS) Hanum, Galuh Ratmana; Wahyudi, David Andrian; Pramushinta, Intan Ayu Kusuma
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1509

Abstract

Water pollution occurs due to the inclusion of chemical substances, components in the form of gases, and solvents so that the quality of water becomes polluted and harmful to the life of organisms in the waters, especially in fish farming. Polluted waters can contain heavy metals. fish can be exposed to heavy metals due to polluted waters. Heavy metals with high or low levels can also cause various types of dead water biota. If the fish exposed to heavy metals consumed by humans will over-accumulate or accumulate in the body, thus endangering health. This study was conducted to determine the levels of heavy metals Lead (Pb) and Zinc (Zn) contained in the meat of fish bandeng in the market Kalanganyar Sidoarjo with Atomic Absorption Spectrophotometer (AAS). This research was conducted in Applied Chemistry Laboratory of Medical Laboratory of Faculty of Health Sciences, University of Muhammadiyah Sidoarjo and Chemistry Laboratory of FMIPA Universitas Islam Maulana Malik Ibrahim Malang in February-April 2021. In this study using descriptive cross sectional research type. Data analysis method using measurement and observation as well as descriptive statistical analysis. 20 samples were used. The results of heavy metal levels in this study obtained the highest levels of lead heavy metal (Pb) of 0.354 mg/kg. While the lowest level is 0.062 mg/kg. Zinc (Zn) heavy metal content is highest at 3,132 mg/kg. While the lowest level is 0.606 mg/kg.

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