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Medicra (Journal of Medical Laboratory Science/Technology)
Published by Universitas Muhammadiyah Sidoarjo
ISSN : 25807730     EISSN : 25807730     DOI : https://doi.org/10.21070/medicra
Core Subject : Health,
Health Professions Public Health
Focus : to facilitate scholar, researchers, and lecturers for publishing the original articles of review articles. Scope : Medicra publishes research articles in the field of “medical laboratory (science/technology)” with the following scope: Clinic Chemical Hematology Microbiology Parasitology Immunology Food and beverage analysis Chemical Molecular Diagnostics Toxicology Cytology Histology Epidemiology Laboratory Management Laboratory Quality Control
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 121 Documents
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Identification And Prevalence Of Flea In Feral Cats In Some Markets Sidoarjo District: Identifikasi Dan Prevalensi Pinjal Pada Kucing Liar Di Beberapa Pasar Kabupaten Sidoarjo Purwa, Ajeng Acika; Ardiansyah, Syahrul
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1577

Abstract

Flea is ectoparasite that live outside the body of their host, one of which host is a cat. Flea infestation have potential to cause dermatitis or a abnormality skin known as flea allergic dermatitis. Cat is an animal that often interact with humans, one of them as a pet, so they have potential to transmit flea to human. The purpose of this study was to identify and determine the prevalence of flea in wild cat in several markets. Sample that used in this study are feral cats taken from the Larangan Market and Gading Fajar Market in Sidoarjo distric. The feral cats obtained were then examined for the presence and type of flea. The results of the examination showed that the prevalence of flea infection in feral cats in the Larangan Market was 81.8% and the Gading Fajar Market was 54.5%. The identification result showed that the fleas found in feral cats from the Larangan Market and Gading Fajar Market were all Ctenocephalides felis. The difference in prevalence is caused by the different environmental conditions in the two markets. A dirty environment has the potential to increase the number of cats infected with fleas.
Effectiveness of The Antibacterial Activity on Orthosiphon aristatus Leaves Extract Against Proteus mirabilis and Staphylococcus saprophyticus: Uji Efektivitas Antibakteri Ekstrak Daun Kumis Kucing (Orthosiphon aristatus) Terhadap Bakteri Proteus mirabilis dan Staphylococcus saprophyticus Nisak, Khairun; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1582

Abstract

Orthosiphon aristatus is traditional medicinal plants. Orthosiphon aristatus leaves contain alkaloid, saponin, steroid, triterpenoid, and phenolic which are antibacterial compounds. Proteus mirabilis and Staphylococcus saprophyticus can cause urinary tract infections. This study aims to determine the optimum concentration of Orthosiphon aristatus leaves extract against Proteus mirabilis and Staphylococcus saprophyticus. This research is an experimental study. This research was carried out in the Bacteriology laboratory of the Muhammadiyah University of Sidoarjo. Antibacterial activity test of Orthosiphon aristatus leaves extract used the disc diffusion method at a concentration of 25%, 50%, 75%, 100%. This test is performed 3 times. Measurement of the diameter of the zone of inhibition is measured using a caliper. The data obtained were tested statistically with Anova with a significant level of 0.05 (5%). The results showed that the Orthosiphon aristatus leaves at a concentration of 25%, 50%, 75%, 100% had a significant effect on Proteus mirabilis with an average of 6.22 mm, 9.36 mm, and 15.55 mm, 21.22 mm and Staphylococcus saprophyticus with a mean of 5.4 mm, 8.8 mm, 14.53 mm, and 20.71 mm.
Analysis of Balanced Diet Index Score with Fasting Blood Glucose in Adult in SDN X Sidoarjo: Analisis Skor Indeks Gizi Seimbang dengan Nilai Glukosa Darah Puasa pada Usia Dewasa di SDN X Sidoarjo Nuriannisa, Farah; Yuliani, Kartika; Nidianti, Ersalina; Rizkiyah, Mubarokator; Santoso, Rizma Defva
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1611

Abstract

Adherence to Pedoman Gizi Seimbang is one of important part of non-communicable diseases prevention. Diet quality and quantity need to be assessed, thus it can detect the risk of non-communicable diseases. Healthy Eating Index (HEI) has been used in many previous studies as an indicator to examine the relationship between diet quality and disease biomarker, like fasting blood glucose (FBG). In Indonesia, the HEI has been modified to Indeks Gizi Seimbang (IGS). However there is no study that examine the relationship between IGS score and biomarker such as FBG yet. This study objective was to analyze the correlation between IGS score and FBG in adult in SDN X Sidoarjo. A cross sectional study design with 30 respondents. IGS4-105 score was measured by 2-days repeated 24h-recall, while FBG by using Glucostick method. Mann Whitney and Spearman test were performed to analyze the study results. The respondents' vegetable intake was relatively low (55.4 grams/day), while the intake of total fat (32.6%-e), saturated fat (16.7%-e), and added sugar (6.8%-e) were excessive. The intake of vegetables and non-dairy animal foods was significantly different between normal FBG group and high FBG group (respectively p value: 0.046, 0.011). The mean score of IGS4-105 was 52.3. There was no significant relationship between the IGS4-105 score and the FBG, but a positive relationship was found between the IGS score for animal foods and the FBG (r = 0.38; p = 0.04). A higher FBG was found in the group with a higher animal food IGS score.
The Relationship between Microalbumin Levels and HbA1c in People at Risk for Type 2 Diabetes Mellitus: Hubungan Kadar Mikroalbumin dan HbA1c pada Orang dengan Resiko Diabetes Mellitus Tipe 2 Romli , Leo Yosdimyati
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1612

Abstract

Increased blood glucose levels in diabetes mellitus are a problem due to poor insulin synthesis, and insulin resistance conditions cause microalbumin. The purpose of this study was to determine the relationship between microalbumin levels and HbA1c in people at risk for type 2 diabetes mellitus. This type of research is a quantitative study using a cross-sectional study design. Respondents in this study were all women at risk of type 2 diabetes mellitus in the village of Ngrandulor. The research sample was taken using the purposive sampling technique, which was taken according to the criteria set by the researcher so that 28 respondents were selected. Data collection on microalbumin levels was carried out by immunoturbidimetric examination, and HbA1c was carried out by immunoassay examination. Bivariate analysis of the research data was carried out using the Spearman rank statistical test. The results showed a relationship between microalbumin levels and HbA1c in people at risk for type 2 diabetes mellitus, the p-value = 0.010. The results showed that half of the respondents had their albumin checked under microalbumin conditions, namely 14 respondents (50%) and on HbA1c examination, which showed that they were in diabetes status or conditions, namely 14 respondents (50%). This study concludes that there is a relationship between microalbumin levels and HbA1c in people at risk for type 2 diabetes mellitus, with a significance value (p) of 0.010.
The Effect of Storage Time for Pooled Sera on Freezers on the Quality of Clinical Chemical Examination: Pengaruh Lama Penyimpanan Pooled Sera pada Freezer Terhadap Mutu Pemeriksaan Kimia Klinik Maulidiyanti, Ellies Tunjung Sari; Purwaningsih, Nur Vita; Widiyastuti, Rahma; Samsudin, Rinza Rahmawati; Arimurti, Anindita Riesti Retno
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1613

Abstract

A common problem in clinical laboratories is maintaining the stability of serum analytes during sample storage. Samples are usually stored in the door (4–8°C) of the refrigerator for a short period of time or in the freezer (-20°C) for a longer period of time (pre-analytical stage) and subsequently, during post-analysis sample handling (storage time and temperature). This study aims to analyze the stability in this case is the storage time of pooled sera in the freezer at temperatures up to -18º C and in the refrigerator at temperatures up to 4°C for 3 months with the inspection parameters, namely cholesterol and triglycerides. This type of research is experimental, with a research design that is time series control group design. The sample in this study was a control serum in the form of pooled sera stored in the freezer and refrigerator which was checked for cholesterol and triglyceride levels every 2 weeks for 3 months or for 12 weeks. The results showed that the average calculation of cholesterol levels in pooled sera stored in the freezer was 142.07 mg/dL and the average cholesterol level examination stored in the refrigerator was 161.89 mg/dL then the average the examination of triglyceride levels in pooled sera stored in the freezer is 112.89 mg/dL and the average examination of triglyceride levels stored in the refrigerator is 140.46 mg/dL. Based on the analysis of the research results, it can be concluded that, there is an effect of storage time in the freezer on the stability of cholesterol levels and triglyceride levels.
Before and After Convalescent Plasma Therapy for Covid-19 Patients at Jemursari Islamic Hospital : Perbedaan Kadar D-Dimer Plasma Sebelum dan Sesudah Pemberian Terapi Plasma Konvalesen Pada Pasien Covid-19 di Rumah Sakit Islam Jemursari Rachmayanti, Notrisia; Bermawi, Bastiana; Nugraha, Gilang; Ballihgoo, Hamiduumajid Ballihg
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1614

Abstract

The World Health Organization declared Covid-19 as a Public Health Emergency of International Concern in January 2020. Treatment options for Covid-19 are still limited, so the health system is looking for a strategy to deal with Covid-19 by using convalescent plasma therapy. This study aims to analyze differences in plasma D-Dimer levels before and after convalescent plasma therapy. This study is an analytic observational study with a retrospective design approach. D-Dimer data were taken from medical records of Covid -19 patients who received convalescent plasma therapy and were treated at Jemursari Islamic Hospital from January to March 2021. A total of 117 patients received convalescent plasma therapy and D-Dimer examinations were performed. Plasma D-Dimer levels were analyzed using statistical tests and the result was p = 0.227. The results of p > 0.05 can be concluded that there is no difference between plasma D-Dimer levels before and after convalescent plasma therapy in Covid-19 patients treated at Jemursari Islamic Hospital.
Description of Hematological Routine in Patients Infected Covid-19 Before and After Convalence Plasma Therapy: Gambaran Hematologi Rutin Pada Pasien Terinfeksi Covid-19 Sebelum dan Sesudah Pemberian Terapi Plasma Konvalesen Ariza, Desyani; Ferdhyanti, Andi Ulfa
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1615

Abstract

Hematological examination is very important and is often requested in several laboratories to determine the state of blood and its components. The purpose of this research is to find out the routine hematological description in patients infected with Covid-19 before and after administration of convalescent plasma therapy. The design of this study used a descriptive method with a sample population of patients infected with Covid-19 at Dr. Hospital. Wahidin Sudirohusodo. This study was followed by 17 subjects who met the inclusion criteria. Based on the results of the study, there were 6 patients who increased leukocyte examination before therapy and after therapy there were 7 patients. There were 2 patients who decreased erythrocyte examination before therapy and after therapy there were 4 patients. There were 3 patients who decreased hemoglobin before therapy and 6 patients after therapy. There was no change in the decreased hematocrit examination, namely 7 patients before and after therapy. Examination of decreased platelets before therapy there are 3 patients and after therapy 4 patients and increased platelets before therapy there are 2 patients and after therapy 3 patients. Neutrophil examination showed that there were no patients who decreased before therapy and there was 1 patient whose neutrophils decreased after therapy. For neutrophils that increased before therapy there were 9 patients and after therapy there were 7 patients. There were 10 patients who decreased lymphocyte examination before therapy and after therapy there were 9 patients. The conclusion was a change in the value of routine hematological parameters before and after convalescent plasma therapy in patients infected with Covid-19.
Study of Creatinin Levels of Patients Receiving Antipsycotic Treatment at Mutiara Sukma Psychiatric Hospital, Nusa Tenggara Barat: Studi Kadar Kreatinin Pasien Jiwa yang Menerima Pengobatan Antipsikotik di Rumah Sakit Jiwa Mutiara Sukma, Nusa Tenggara Barat Sukmana, Dhika Juliana; Mentari, Ika Nurfajri; Anjelin, Desak Putu
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1616

Abstract

Mental disorders are clinical and social problems that must be addressed immediately. Long-term use of the drug can cause damage to kidney function and worsen the work of the kidneys. Creatinine is a waste product of creatine phosphate metabolism that occurs in the muscles and is then released into the blood circulation, then transported to the kidneys. The purpose of this study was to determine how the creatinine levels of mental patients receiving antipsychotic treatment at Rumah Sakit Jiwa Mutiara Sukma Hospital, Nusa Tenggara Barat Province. The research method used is qualitative. The type of research used is descriptive, namely looking at data from the Clinical Pathology laboratory installation and medical records to determine the results of examination of creatinine levels in patients with mental disorders. The results showed that from data in the Clinical Pathology laboratory and medical records at Rumah Sakit Jiwa Mutiara Sukma, NTB Province from June-December 2020 with a total of 40 data, there were 30 patients (75%) who had normal creatinine levels, 2 patients (5%) had normal creatinine levels. creatinine levels were low, and 8 patients (20%) had high creatinine levels.
Heavy Metal Content in Hair at Workers in Gas Station of Sidoarjo City in 2021: Kandungan Logam Berat pada Rambut Petugas Stasiun Pengisian Bahan Bakar Umum (SPBU) di Sidoarjo pada Tahun 2021 Rohmah, Jamilatur; Miranda, Niken Anggita; Marlina, Mariany Lambertine; Fiya, Uus Ufita
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1617

Abstract

One of the most dominant workplaces where heavy metal contamination occurs is the Gas Station (SPBU). Gas station workers are at high risk of being exposed to heavy metals while working due to inhaling fuel that evaporates into the air and vehicle fumes due to the behavior of motorists who do not turn off the engine while waiting in line. Determination of the level of heavy metal contamination of Zn, Cu, Pb, Cd, Fe, and Cr was determined through hair clippings using the Atomic Absorbtion Spectrophotometry (AAS) method. The gas stations used in this study are SPBU X and Y in Sidoarjo. This study aims to determine the levels of heavy metals in hair and to determine the relationship between working period, age, smoking habits, and savety with heavy metal levels in gas station workers in Sidoarjo. The type of research used is descriptive cross sectional and analytical method through filling out questionnaires and correlative statistics with SPSS. The results obtained showed that the average levels of heavy metals Zn, Cu, Pb, Cd, Fe, and Cr at SPBU X were 0.232, respectively; 0.000; 0.003; 0.001; 0.464; and 0.024 mg/g. while the average levels of heavy metals Zn, Cu, Pb, Cd, Fe, and Cr at SPBU Y were 0.516, respectively; 0.000; 0.007; -0.001; 0.345; and 0.051 mg/g. It can be said that the levels of heavy metals Zn, Cu, Pb, Cd, Fe, and Cr did not exceed the specified threshold. The results of the statistical test of the correlation between the levels of heavy metals Zn, Cu, Pb, Cd, Fe, and Cr with years of service, age, smoking habits, and PPE of gas station workers were not significant (p>0.05).
Effects of Giving Alfamangios on Cholesterol Levels and Pancreatic Appearance in Diabetes Mellitus Induced Rats : Efek Pemberian Alfamangios Terhadap Kadar Kolesterol Dan Gambaran Pankreas Pada Tikus Terinduksi Diabetes Mellitus Kardina, Rizki Nurmalya; Santoso , Andreas Putro Ragil; Putri, Fildzah Karunia; Murtasiyah, Erna Dwi; Arif, Sheila Weni Astuti
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1618

Abstract

The isolate compound α-Mangostin was the most dominant compound found in the rind and trunk of the mangosteen fruit tree (Garcinia mangostana L.). These compounds are reported to have many pharmacological activities such as antibacterial, antifungal, antidiabetic, anti-cancer and several other bioactivities. The aim of this research was to measure the activity of α-Mangostin isolate using 13C and 1H NMR and to find out whether this compound had bioactivity on pancreatic physiology and histopathology in type II diabetes mellitus rats with the parameters measured were cholesterol and pancreatic histopathology. This research was conducted at the Laboratory of the Faculty of Veterinary Medicine, Airlangga University, Surabaya. This research is a true experimental type because the data were taken through laboratory tests with the treatment of the research sample. The sample in this study were rats with induced diabetes mellitus. 30 rats were separated into 5 groups for each group of 6 rats. The results of this study There are differences in the effect of giving alpha mangiostin on blood cholesterol levels in rats that have been induced by diabetes mellitus, and there is a change in the morphology of the pancreas caused by free radicals.

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