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Arif Rahman Hikam
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Fakultas Biologi Universitas Jenderal Soedirman Jl. dr. Soeparno No. 63 Purwokerto, Kabupaten Banyumas Kode Pos 53122
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
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Search results for , issue "Vol 4 No 3 (2022): BioEksakta" : 8 Documents clear
STRUKTUR KOMUNITAS PLANKTON PADA WAKTU YANG BERBEDA DI TELAGA KUMPE BANYUMAS Nur Haliza, Fitra Dwi; Rahayu, Diana Retna Utarini Suci; Sastranegara, Moh Husein
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4579

Abstract

Trade and tourism activities in Telaga Kumpe can increase the input of organic matter into the waters which results in increased fertility, decreased function and changes in water quality so that it has an impact on the community structure of plankton as one of the aquatic organisms. This study aims to determine the abundance, diversity, uniformity and dominance of plankton before sunrise (morning) and after sunrise (afternoon) as well as differences in the ratio of plankton abundance before sunrise and after sunrise (afternoon) in Kumpe Lake. . The research was carried out at three sampling points, namely at the inlet, in the middle of the lake and at the outlet. The main parameters measured included the number of individuals and plankton species, while the supporting parameters included physico-chemical factors in the waters, namely water temperature, light intensity, depth, pH and TDS levels. The results showed that the highest abundance of phytoplankton were Microcystis aeruginosa and Mougeotia viridis, while the highest abundance of zooplankton were Diaptomus siciloides and Cyclops vicinus. The diversity of plankton in the morning is included in the medium category, in the afternoon the low to moderate category. The uniformity of plankton in the morning and afternoon is in the low to high category. The plankton dominance index value in the morning indicates a dominant species, whereas during the afternoon it does not indicate a dominant species. The dominating species are Microcystis aeruginosa, Mougeotia viridis, Diaptomus siciloides and Cyclops vicinus. The results also show that the abundance of phytoplankton is higher during the day, and the abundance of zooplankton is higher in the morning
Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus Kurniawati, Pratiwi Kusuma; Ryandini, Dini; Hernayanti, Hernayanti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4593

Abstract

The genus Streptomyces is known as producers of bioactive compounds that have antibacterial and antioxidant properties. Streptomyces sp. SAE4034 was known to produce antibacterial compounds that can inhibit and kill pathogenic bacteria, including Staphylococcus aureus, but the group of antibacterial compounds and their inhibitory mechanisms were not yet known. The ability of Streptomyces sp. SAE4034 in producing antioxidants is unknown yet. This research aimed to determine the classification of antibacterial compounds produced by Streptomyces sp. SAE4034, its potency in producing antioxidant compounds, and to observe the inhibitory mechanisms of antibacterial compounds produced by Streptomyces sp. SAE4034 against S. aureus using cell leakage method. The results of this research showed that Streptomyces sp. SAE4034 produce compounds in the groups of alkaloid, terpenoid, flavonoid, and polyphenol. Compounds with Rf values 0.40 and 0.47 which includes into polyphenol and 0.72 which includes into alkaloid group has the ability to inhibit the growth of S. aureus. The MIC value was 256 μg/mL. Streptomyces sp. SAE4034 has a very strong antioxidant activity that can scavenge DPPH free radical with IC50value of 32,14 ppm and has an antioxidant enzyme activity that is superoxide dismutase amount of 63,10 U/mL The inhibition mechanism of Streptomyces sp. SAE4034 crude extract against S. aureus was through leaking of the cell wall or cell membrane resulting in the release of nucleic acid and protein of S. aureus cells and the highest cell leakage was at 2 doses of MIC.
Morfometri dan Meristik Ikan Gabus (Channa striata) dari Sungai Klawing Kabupaten Purbalingga Jawa Tengah Putra, Dwi Nofyan Sansa; Bhagawati, Dian; Setyaningrum, Nuning
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4681

Abstract

Peningkatan permintaan ikan gabus yang tinggi dari masyarakat menyebabkan terjadinya eksploitasi yang tinggi pada ikan gabus (C.striata). Kegiatan penangkapan ikan gabus yang tinggi seiring dengan volume produksi yang meningkat setiap tahun tentunya akan mengancam kelestarian ikan gabus. Perlunya dilakukan penelitian morfometrik dan meristik yaitu agar dapat memberikan informasi yang dapat digunakan untuk upaya pelestariannya di Sungai Klawing. Penelitian ini bertujuan untuk mengetahui apakah morfometri standar, truss morphometric, dan meristik dapat digunakan untuk mengidentifikasi jenis kelamin ikan gabus dari Sungai Klawing dan mengetahui karakter spesifik yang membedakan morfologi ikan gabus jantan dan betina. Metode yang digunakan adalah survei dengan pengambilan sampel secara accidental sampling dari Sungai Klawing yang dibantu oleh penangkap ikan. Pengambilan sampel dilakukan sebanyak 5 kali dari bulan Februari hingga Maret 2021. Variabel yang diamati adalah performa morfologi, morfometri standar, truss morphometric, dan meristik. Parameter pada penelitian ini adalah bentuk tubuh, bentuk mulut, posisi mulut, bentuk sirip ekor, tipe sisik, tipe gigi, rasio jarak-jarak bagian tubuh, jumlah jari-jari pada sirip, dan jumlah sisik. Data performa morfologi, morfometri standar, truss morphometric, dan meristik dianalisis secara deskriptif. Hasil penelitian diketahui bahwa performa morfologi tidak dapat digunakan sebagai pembeda jenis kelamin ikan gabus. Karakter morfometri standar yang dapat digunakan sebagai pembeda jenis kelamin ikan gabus dari 21 karakter yaitu lebar kepala, dengan nilai asymp.sig 0,20. Karakter truss morphometric yang dapat digunakan sebagai pembeda jenis kelamin ikan gabus dari 23 karakter yaitu A1 dengan nilai asymp.sig 0,048, A6 dengan nilai asymp.sig 0,038, C5 dengan nilai asymp.sig 0,021. Karakter meristik tidak dapat digunakan sebagai pembeda jenis kelamin ikan gabus, karena memiliki jumlah jari-jari sirip dan sisik yang relatif sama.
Diferensial Leukosit Beberapa Spesies Ikan Tangkapan Dari Familia Cyprinidae Di Sungai Banjaran, Kabupaten Banyumas Irwanti, Nabilla Gina; Susatyo, Priyo; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4703

Abstract

Familia Cyprinidae dapat ditemukan di hampir seluruh sungai Indonesia termasuk Sungai Banjaran, Kabupaten Banyumas. Diferensial leukosit bertujuan mengetahui perbedaan persentase sel leukosit. Penelitian ini untuk mengetahui hubungan antara lokasi pengambilan sampel (hulu, tengah, dan hilir) dengan diferensial leukosit dan mengetahui gambaran apusan darah dari beberapa spesies ikan familia Cyprinidae. Pengambilan sampel menggunakan teknik purposive sampling pada tiga stasiun yang berbeda. Data yang diperoleh dianalisis dengan metode Analysis of Variance (ANOVA) dengan tingkat kesalahan 5% dan gambaran hasil pengamatan mikroskopis jenis-jenis leukosit. Hasil penelitian menunjukkan bahwa perbandingan persentase sel lekosit pada Ikan Nilem (Osteochilus vittatus), Ikan Brek (Barbonymus balleroides), dan Ikan Lunjar (Rasbora argyrotaenia) dengan lokasi Hulu, Tengah, Hilir Sungai Banjaran, Banyumas, berbeda secara signifikan (p<0,05). Persentase sel leukosit yang paling tinggi ada pada zona Hilir di Sungai Banjaran, Banyumas yaitu sel neutrofil dan sel monosit. Presentase sel neutrofil Ikan Nilem didapatkan sebesar 9,5%, Ikan Brek sebesar 8,5% dan Ikan Lunjar sebesar 9,4%. Presentase sel monosit pada Ikan Nilem sebesar 8,9%, Ikan Brek sebesar 8,7% dan Ikan Lunjar sebesar 8,5%. Sel neutrofil memiliki sitoplasma bergranula halus dan ditengahnya terdapat inti bersegmen. Sel eosinofil mempunyai sitoplasma berwarna lebih merah, inti yang terletak memanjang di tepi sel, memiliki granula besar. Sel limfosit memiliki sitoplasma berwarna biru gelap dan inti berbentuk bulat hingga oval. Sel monosit memiliki sitoplasma berwarna biru pucat dan bentuk inti bervariasi ada yang berbentuk seperti ginjal hingga yang berbentuk dua lobus besar.
Keanekaragaman Morfologis Sukun [Artocarpus altilis (Park.) Fosberg. var. non-seminiferus] Di Daerah Banyumas Rizkyana, Risna; unsoed, sukarsa; Widodo, Pudji; Palupi, Dian
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4731

Abstract

Breadfruit [Artocarpus altilis (Park.) Fosberg] is a plant that have different morphological variations in each region. The Banyumas area is an area that is very suitable for the growth of breadfruit. This study was aimed to determine the diversity of breadfruit plants based on their morphological characteristics. This research was conducted using a survey method, then the data were analyzed descriptively. The research variable was the morphological characteristics of the breadfruit plant, the parameters were the morphological of the canopy, buttress roots, stems, leaves, flowers, and fruits. The results showed that the morphological variations of breadfruit were found in the shape of the canopy varies, namely broad pyramid, pyramid, ovoid, round, and irregular, some have buttress roots and none, fruit shape varies, namely round and oval. Leaf blade length varies between 26-56 cm, leaf blade width varies between 20-45 cm, leaf stalk length varies between 3-6 cm, leaf shape is ovoid, upper leaf surface is smooth, lower leaf surface is rough, leaf edge incised, the tip of the leaf is tapered, the base of the leaf is wedged, the color of the leaf is dark green. Male flower length varies between 9-17 cm, male flower color varies, namely light green and brownish, male flower shape resembles a club, female flower diameter varies between 5-6 cm, female flower shape is round, female flower color is light green. Fruit weight varies between 450-1250 g, fruit diameter varies between 9-18 cm, fruit skin color is green, flesh color is white to yellowish white, flesh texture is smooth, fruit skin pattern is polygonal, and seeds are round, black.
Prevalensi dan Kelimpahan Ektoparasit pada Ikan Belanak (Mugil Cephalus) Hasil Tangkapan di Muara Sungai Serayu Adipala Cilacap Pajrulloh, Ade Rizki; Rokhmani, Rokhmani; Riwidiharso, Edy
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4733

Abstract

Belanak (Mugil cephalus) is a fish that can adapt to a wide salinity 17-30% and its distribution in tropical to subtropical areas, so that mullet can be found in rivers to river mouths, one of which is found in the Serayu River Estuary, Adipala Cilacap. Fish diseases can be caused by parasites, one of which is ectoparasites. Ectoparasites are organisms that benefit from infesting the body surface of fish. This study aims to determine the prevalence of mullet infested with ectoparasites and to determine the abundance of ectoparasites in mullet (Mugil cephalus) in the Serayu Adipala River, Cilacap. The research method used is survey method with purposive random sampling technique. The variables observed were the prevalence and abundance of ectoparasites. The parameters observed were the type and number of ectoparasites found in mullet. The results showed that the prevalence of ectoparasites in mullet, which was 31±13.216%, was included in the general infestation category. The types of ectoparasites found were Dactylogyrus sp. and Apiosoma sp. The abundance of ectoparasites found in mullet is Dactylogyrus sp. of 0.25±0.14 ind/head and Apiosoma sp. of 0.36± 0.14 ind/head. Both results included in the category of very rare infestations and the highest ectoparasites found in the organs of the mullet fish were found in the gills, namely Dactylogyrus sp. as many as 25 individuals.
Pengaruh Jenis dan Kosentrasi Bahan Tambahan terhadap pertumbuhan Jamur Tiram Coklat (Pleurotus pulmonarius) MU'AFA, RAFID HILMY; Ratnaningtyas, Nuniek Ina; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.5022

Abstract

Brown oyster mushroom (Pleurotus pulmonarius) is one type of wood mushroom that can be cultivated, but it is not yet popular for oyster mushroom cultivators and consumers in Indonesia. Planting medium materials give different results, depending on the ratio of the given composition. This study aims to determine the effect of different types and concentrations of additives on the growth of brown oyster mushrooms and to determine the best type and concentration of additives for the growth of chocolate oyster mushrooms. This study used a Factorial Completely Randomized Design (CRD) consisting of the following treatments: type of additives, namely rice bran, corn flour, polar, and wheat flour, as well as different concentration factors of additives, namely 0%, 5%, 10%, 15%, and 20%. Each treatment was repeated 4 times, so there were 80 experimental units. The independent variables in this study were the type and concentration of additives, while the dependent variable was the growth rate of fungal mycelium. The main parameters observed were mycelium growth and fruit body weight of brown oyster mushroom (P. pulmonarius), while the supporting parameters were the appearance of the first fruiting body, mycelium density, incubation room temperature, and pH of the growing medium. The data obtained were analyzed using analysis of variance (ANOVA) with an error rate of 5% and followed by Duncan's Multiple Range Test (DMRT) with an error rate of 5%. The results showed that the interaction between the type and concentration of additives affected the production of brown oyster mushrooms (P < 0.05). The type of rice bran additive with a concentration of 20% showed the best results, namely 0.97 cm/day for mycelium growth rate and 0.885 kg for brown oyster mushroom fruiting body weight.
APLIKASI NAA PADA PROPAGASI KANTONG SEMAR Nepenthes adrianii Batoro, Wartono & Jebb Wahdani, Rosa Amalia; Dwiati, Murni; Kamsinah, Kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.5481

Abstract

Nepenthes adrianii is an endemic species of Mount Slamet. The existence of this species has begun to be threatened. This situation encourages efforts to conserve N. adrianii, which is through propagation using stem cuttings of N. adrianii. In order for the propagation of stem cuttings to grow properly, it is necessary to add growth hormone (ZPT) from the auxin group such as NAA (Napthalene Acetic Acid). This research has been conducted to study the effect of NAA and its effective consentration for the growth of N. adrianiistem cuttings. This research was conducted using experimental methods. The experimental design used was a Randomized Completely Block Design (RCBD). The block in this study is total leaves per cuttings. Block 1 is the leafless cuttings, block 2 is cuttings with one leaf, block 3 is cuttings with two leaves, and block 4 is cuttings with three leaves. The NAA concentrations in this study are 0, 5, 10, 15, 20, and 25 ppm. There are a total of 24 experimental units. The variable observed was the growth of N. adrianii stem cuttings. Parameters observed in this study are days taken for sprouting, number of shoots, number of leaves, leaf length, and leaf width. The results were analyzed using Variety Analysis and then analyzed with Tukey's test at the test level of 0.05 at the time of significant treatment. The results showed that the application of NAA did not affect the growth of the stem cuttings of N. adrianii. The best concentration of NAA could not be determined because there was no effect of NAA on the growth of N. adrianii stem cuttings.

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