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MEDIA PETERNAKAN - Journal of Animal Science and Technology
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Core Subject : Agriculture,
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Articles 522 Documents
Identifikasi Genetik Menggunakan Marker Mikrosatelit dan Hubungannya dengan Sifat Kuantitatif pada Sapi . Maskur; . Muladno; B Tappa
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The occurrence of genetic changes in Bali and Brangus cattle crossing and it's relationship to quantitative traits (e.g. growth and birth weight) were identified using microsatellite markers. Sixteen microsatellite loci were amplified using polymerase chain reaction (PCR) technique, the amplified products were then separated on vertical discontinuous polyacrylamide gel electrophoresis (PAGE) and silver-staining method was applied to detect the fragment. The evaluation to the stability and allele inheritance pattern were checked using Chi-Square segregation analysis. The results of this research showed that allele numbers average detected in Brangus and Bali cattle and their progeny were 2.31, 2.56, and 2.75 respectively. At most microsatellite loci, the allele distributions incline to concentrate and to form bimodal trend. Of 16 micro satellite loci tested, 5 microsatelitte markers namely INRA 037, HEL 9, CSSM 66, INRA 035 and ETH 225 indicated a significant response to average daily gain. In terms of birth weight, a significant response was shown by INRA 037. Key word: microsatellite, PCR, genotype, inheritance, allele
Seleksi Burung Puyuh Generasi II Berdasarkan Bobot Badan dan Perubahan Biokimia Genetika S M Ardiningsasi
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The birds were developed to be 16 groups (4 groups of TT and 4 groups of RR, with male and female lines, respectively). The mating was conducted between similar groups of population (TT vs. TT, and RR vs. RR) to obtain second generation. The change in metabolism such as Ca-ATPase activity and Nô-œmethylhistidine (Nô-MH), also selection response between generations were analyzed. Parameters of metabolism were subjected to statistical analysis of T-test to compare between productions characteristic (TT and RR), especially for second generation. Body weight was also statistically analyzed by the same method. Selection response in TT group (3.10% or 2.74%) was higher than that in RR group (1.20% or 1.13%). Metabolism aspect in the quails of second generation either rate of protein turnover or activity of bone Ca-ATPase enzyme showed the change toward the productivity specification. Rate of muscle protein synthesis was higher and enzyme activity of Ca-ATPase was lower in group of TT population than those in group of RR population. Key words: quail, breeding, body weight, protein turnover, Ca-ATPase
Penggunaan Pupuk Kandang pada Padang Rumput di Lahan Kering Sulawesi Tenggara Harapin Hafid H; . Darwis; M Jaya
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Generally, the permanent pasture of dry farming in South East Sulawesi is dominated by the perennial grass and coppi plant, which have very low palatability and benefit for livestock. The aim of the research was to increase the productivity of pasture in South East Sulawesi by introducing perennial grasses and manure application. The experiment was conducted in UPTD and analytical laboratory of FMIPA from April to September 2006 using three types of perennial grasses i.e. Brachiaria decumbens, Paspalum dilatatum and Cynodon plectostachyrus. Perception conducted to calculate the botanical composition and soil properties before and after the treatment. The field experiment was carried out as two factorial experiment arranged in Randomized Block Design. The first factor was the species of perennial grass and the second factor was different level of manure fertilizer. The result of the experiment showed that the crude fibre content of B. decumbens was lower than those of P. dilatatum and C. plectostachyrus. The crude fibre content increased with the increasing manure application. But the protein content of B. decumbens was higher than those of P. dilatatum and C. plectostachyrus. It is suggested to plant B. decumbens to get higher quality forage for livestock in South East Sulawesi. Key words: manure, perennial grass, pasture, protein, fiber
Karakteristik Semen Segar dan Kualitas Semen Cair Kuda dalam Pengencer Dimitropoulos yang Disuplementasi dengan Fruktosa, Trehalosa dan Rafinosa . Yudi; I Arifiantini; B Purwantara; T L Yusuf
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The objective of the experiment was to study the characteristics of stallion fresh semen and the quality of sperm preserved in Dimitropoulos extender (DV) supplemented with different concentration of fructose, trehalose and raffinose. Semen were collected using artificial vagina from three stallions. Semen characteristics and quality were evaluated macro- and microscopically. Prior to extension, semen were centrifugated at 3000 rpm for 20 minutes. The condensed sperm were re-suspended in DV supplemented with different types of carbohydrate to meet the concentration of 200 million spz/ml. All samples were stored at room and chilled temperature, and were evaluated for motility and viability every 3 h and 12 h. The results of the experiments indicated that fresh semen characteristics were fair good; the volume, consistency, motility, live-dead ratio, concentration (106/ml), total spermatozoa (109/ejaculate) and abnormality were 29.25±9.33 ml, watery, 7.00±0.12, 67.08±9.08%, 77.89±6.46%, 211.88±21.15, 6.28±2.45 and 27.26±4.64%, respectively. The supplementation of different type and concentration of carbohydrates did not significantly affect the motility and viability. However, the supplementation of 50 mM fructose significantly increased the motility and viability of the sperm compared to the control. In conclusion, carbohydrate supplementation in DV may not maintain the sperm quality, particularly in the medium with the osmolarity higher than 400 mOsm/kg. Key words: stallion, semen, Dimitropoulos, carbohydrate
Suplementasi Nutrien Defisien untuk Meningkatkan Penggunaan Daun Rami (Boehmeria nivea, L. Gaud) dalam Ransum Domba . Despal
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

A research to improve ramie leaf utilization in local sheep ration by supplementation of deficient nutrients was conducted. Four rations as treatments were tested using 12 local sheep (16.5 ± 1.6 kg LW) in a block randomized design. The treatments were T1 = 3% BW of DM native grass; T2 = T1 + 0.75% BW (25% of DM native grass) of DM ramie leaf; T3 = T2 + supplements Cu, P and methionine; T4 = T1 + 1.5% BW (50% of DM native grass) of DM ramie leaf + supplements Cu, P and methionine. Ration consumptions and digestibility, plasma level of Ca and P and body weight changed were determined. Dry matter intake increased in line with level of ramie leaf offered and there were no significantly difference on digestibility between the rations. Native grass alone failed to give a positive body weight change of local sheep. Addition of 25% DM ramie to native grass (T2) increased intake of digested nutrient and reduced body weight loss. Addition of supplement (T3) at the same level of ramie leaf increased intake of digested nutrient and changed the curve slope to the positive direction after the third week of observation. Addition of 50% DM ramie leaf to native grass basal ration followed by supplementation (T4) improved intake of digested nutrient closed to recommended nutrient requirement and showed a positive body weight changed. Key words: Boehmeria nivea, nutrient deficiency, sheep
Penggunaan Tepung Limbah Udang yang Diolah dengan Filtrat Air Abu Sekam dalam Ransum Ayam Broiler . Mirzah
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The objective of this study was to measure the effect of different levels of shrimp head waste (SHW) substituting fish meal (FM) in broiler diets. FM is the sole crude protein from animal sources. A control fish meal broiler diet and four different levels of SHW substituted for crude protein FM were fed to CP 707 Arbor Acres broiler strains from day old chick to four weeks of age. The crude proteins FM were replaced with 0 (R0); 25 (R1); 50 (R2); 75 (R3); and 100 (R4) percent of crude protein SHW. The five treatments were assigned to completely randomized design. Feed consumption, body weight gain, feed conversion and carcass percentage were recorded to measure the performances. The results of Duncan's revealed that feed consumption, feed conversion and carcass percentage were not significantly different with increasing level of SHW as substituted crude protein FM in broiler diets. However these parameters in bird fed 100 % SHW diets did not differ from those in birds fed 16 % FM (7.32% crude protein from FM). While body weight gain decreased with increasing levels of SHW in broiler diets (R4). The decrease body weight gain may be due to the decreased feed intake and amino acid in balance and the increased chitin content in the diet. The conclusion of this experiment that SHW can be used as a protein source in broiler diets up to 75% to replace FM. Key words: shrimp head waste meal, fish meal, broiler, performances
Morfometrik Usus dan Performa Ayam Broiler yang Diberi Cekaman Panas dan Ekstrak n-Heksana Kulit Batang “Jaloh" (Salix tetrasperma Roxb) . Sugito; W Manalu; D A Astuti; . Chairul
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The n-hexane fraction of “jaloh" extract could reduce detrimental effects of heat stress on broiler chicken. An experiment was conducted to study morphometric of small intestine villi (duodenum, jejunum, and ileum) and performances of broiler exposed to heat stress on 33±1oC for 4 hours/day and given extract n-hexane of “jaloh" bark (EHJ) with doses 5, 10, and 20 mg/kg body weight (BW). Thirty broilers aged 20 days (Cobb strain) were randomly divided into 5 groups of treatments. The first treatment group was external control namely chickens without heat stress and without EHJ administration (tCP). The second group of treatment was internal control representing chickens exposed to heat stress without EHJ administration (CP). The third, fourth, and fifth treatment groups consisted of chickens exposed to heat stress and given 5 (CP+EHJ5), 10 (CP+EHJ10), and 20 mg/kg BW of EHJ (CP+EHJ20), respectively. Heat stress and “jaloh" extract were implemented every day. “Jaloh" extracts were given 1 hour before temperature in the cage was raised. Results showed that heat stress decreased weight gain and height of jejunum villi, and increased feed conversion ratio. The dose of 10 mg/kg BW of EHJ can improve performance of broiler chickens and morphometric of small intestine villi. Key words: heat stress, Salix tetrasperma, performance, broiler
Strategi Suplementasi Protein Ransum Sapi Potong Berbasis Jerami dan Dedak Padi B.W H.E Prasetiyono; . Suryahadi; T Toharmat; R Syarief
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

ice straw and bran are low in protein. This study examined protein supplement (SPN) composed of CASREA (cassava-urea complex) and SOYXYL (protected-protein soybean meal) in rice straw and bran based ration offered to beef cattle. Experiment 1: Casrea1 (no extruded 32% urea and 58% cassava), Casrea2 (extruded 22% urea and 68% cassava), Casrea3 (extruded 27% urea and 63% cassava), and Casrea4 (extruded 32% urea and 58% cassava) were incubated in ruminal fluid. Experiment 2: Protected-protein soybean meal with xylose from Black Liquor’s (BL) of 0, 3, 6, or 9% and extruded at 120, 150, or 180oC, were incubated in ruminal fluid. Experiment 3: The best treatments of experiments 1 and 2 were used as SPN. Sixteen dairy cattle bulls aged 12-15 months were divided into 4 blocks to receive one of the following treatments: R0= rice straw and bran, R1= R0 with SPN A, R2= R0 with SPN B, R3= R0 with SPN C. SPN A, B and C composed of CASREA:SOYXYL in ratio of 20:80, 50:50, and 80:20, respectively. Casrea 2 had the highest microbial protein and post rumen protein digestibility of 29.04 mg and 76.16%, respectively. Protected-protein soybean meal with xylose from BL 3% and extruded at 1500C had the highest microbial protein and post rumen protein digestibility. SPN increased dry matter, organic matter and protein intake and their digestibility, ration efficiency, and daily gain. The highest daily gain (0.85 kg.d-1), ration efficiency (11%), and income over feed cost (Rp 7500 head-1.d-1) were R3. R3 had lower methane energy compare to R0. The result indicated that JDP supplemented with SPN (80% CASREA and 20% SOYXYL) improved ration efficiency and performance of cattle. Key words: rice straw, rice bran, cassava, urea, soybean meal
Analisis dan Simulasi Distribusi Suhu Udara pada Kandang Sapi Perah Menggunakan Computational Fluid Dynamics (CFD) A Yani; H Suhardiyanto; R Hasbullah; B P Purwanto
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

This experiment was conducted to analyze the temperature and relative humidity distribution in dairy barn of Friesian Holstein (FH) using computational fluid dynamics (CFD) as a basic consideration for dairy barn design. The capacity of the dairy barn was 20 heads of FH with tail to tail model. The dimensions of the dairy barn were: 13 m in length, 6.3 m in width, and 5.75 m in height. The floor was made from concrete with 20 slope. Asbestos was used as roof of the dairy barn, whereas frame of the dairy barn was made from steel. The results of the analysis showed that during the daytime, air temperature inside the dairy barn increased by the height from floor level. The CFD simulation showed clearly the temperature distribution in the dairy barn. Air temperature obtained from CFD simulation was in line with that of the measured values. Therefore, it can be used as basic consideration for the dairy barn design with respect to low air temperature and uniform air temperature distribution. It was recommended that one of the best design configurations is 6.25 m high, 8.3 m wide, 0.4 m high of wall. The best design could decrease 0.474 0C of air temperature and increased dry matter intake of dairy cattle 0.403 kg per day per head. The amount of heat production of FH was considered to determine the best design of dairy barn. Key words: dairy barn design, air temperature distribution,computational fluid dynamics (CFD)
Status Ca, Mg dan Zn pada Kambing Peranakan Etawah Muda yang Diberi Ransum Bentuk Mash dengan Pakan Sumber Serat Berbeda T Toharmat; N Hotimah; E Nursasih; R Nazilah; T Q Noerzihad; N A Sigit; Y Retnani
Media Peternakan Vol. 30 No. 2 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Fibrous agricultural by-products are the component of most rations for ruminant raised intensively. Slow rate and low digestibility of the fibrous feed may limit mineral bioavailability. Present experiment aimed to clarify the influence of fibrous feed component in ration on the status of Ca, Mg and Zn in growing goats. Twenty of female Etawah-grade goats weighing of 13.5±2.14 kg were grouped and allocated into five treatments in a randomized block design. Rations composed of 50% fibrous feed and 50% concentrate. The fibrous feed component as treatments were: RG = napier grass, JP = rice straw, KC = cacao pod, JK = mixed rice straw and coffee husk, and CP = mixed rice straw, napier grass, coffee husk and cacao pod. Rations were offered at 3% of live weight. The result showed that fibrous feed influenced Ca, Mg, and Zn intake, Ca and Mg absorption, and Ca, Mg and Zn plasma of growing goats. Intake, absorption and plasma Ca, Mg, and Zn had positive correlation with dry mater intake. Absorption of Ca and Zn had positive correlations with crude fiber digestibility. Supplementation of Ca, Mg and Zn was necessary when the fibrous feed was included at the level of 50% in the ration of growing goats, but the supplement level varied according to the type of the fibrous feed. Key words: fibrous feed, mineral status, Etawah goat

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