Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Jurnal Fitopatologi Indonesia

jfiti
Website

Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

Arjuna Subject : -

Articles 412 Documents

15 16 17 18 19

Cara Preservasi Fitoplasma dari Jaringan Kacang Tanah Bergejala Sapu untuk Deteksi DNA dengan Teknik PCR Pulogu, Siska Irhamnawati; Mutaqin, Kikin Hamzah; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Witches‘ broom of peanut caused by phytoplasma is a common disease found in Indonesia. Phytoplasma is able to be detected using polymerase chain reaction (PCR) technique. One of important factor which determine the successful of phytoplasma amplification is the DNA availability from fresh tissues. The research was aimed to evaluate some preservation methods of phytoplasma from infected plant samples. The aspects to be evaluated consisted of time (1, 2, 3, and 4 weeks), temperature (-20 °C, 4 °C, and 25 °C), and preservation medium (1X PGB buffer, 3 M NaCl, CTAB buffer, 70% ethanol, non medium, and FTA-card) for storing the fresh phytoplasma infected samples. Good preservation method will optimize the phytoplasma DNA amplification using PCR standard technique followed by nested-PCR. The results showed that preservation of samples at -20 °C, 4 °C, and 25 °C in CTAB buffer was able to maintain the tissue freshness for 4 weeks and was able to provide the DNA of either quality or quantity sufficiently for PCR detection. PCR standard using a primer pair P1/P7 showed that not all of the preserved DNA of phytoplasma were amplified positively. However, standard PCR followed by nested-PCR using primer pair fU5/rU3 was able to increase the DNA detectability. Preserved samples derived from various medium and stored for 4 weeks gave positive results. This results were in contrary with previous same samples which were detected negatively by standard PCR technique.

Ketahanan Kultivar Pisang Lokal Kalimantan Selatan terhadap Penyakit Bercak Sigatoka (Mycosphaerella sp.) Mariana, Mariana; Rodinah, Rodinah; Budi, Ismed Setya
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Sigatoka of banana caused by Mycospherella sp. is an important disease, since it causes yield losses. Resistant variety is considered a promising approach for sigatoka disease management. This study was aimed to evaluate the resistance of local banana cultivars from South Kalimantan against sigatoka disease. The fungus was isolated from leaves of Kapas cultivar showing severe symptoms of sigatoka disease, onto potato dextrose agar medium. The isolate was then confirmed as Mycosphaerella sp. following Koch’s postulate. Level of resistance is determined based on symptoms development. Resistance of local banana cultivars can be differentiated into susceptible (‘Awak’, ‘Jaranang’, ‘Kapas’, ‘Mas’, and ‘Paikat’), medium resistant (‘Ambon’, ‘Kepok’, ‘Mas Bantan’, ‘Mauli’, and ‘Talas’), and resistant (‘Tarati’). In general, the symptom was visible 2 to 8 days after inoculation. The longer incubation period the more resistant response of banana varieties. Incubation period was negatively correlated to the disease intensity, but it is not correlated with stomatal density.

Galur Isogenik Xanthomonas oryzae pv. oryzae Hasil Penyinaran Ultraviolet dan Potensinya Sebagai Penginduksi Resistensi Padi terhadap Penyakit Hawar Daun Bakteri Yuliani, Fitria; Giyanto, Giyanto; Mutaqin, Kikin Hamzah
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is an important rice disease and may cause 50% plant damage. One approach to manage this disease is using isogenic non-pathogenic strain that can induce plant resistance. This study was conducted to obtain isogenic strains of X. oryzae pv. oryzae through ultraviolet irradiation and evaluate ability of the isolates in inducing plant resistance. Bacteria X. oryzae pv. oryzae were exposed to UV for 3, 5, 10 and 15 min. A total of 99 colonies obtained were selected through pathogenicity tests, followed by confirmation using XOR-F and XOR-R2 primers. As much as 16 isogenic strain isolates were obtained with the ability to produce exopolysaccharides (EPS) ranges between 1.4–9.8 mg mL-1 compared to wild-type 6.0 mg mL-1. Five isogenic strains isolates (M313, M57, M101, M1513 and M1515) caused virulence reduction of 92.8–98.6%. Application of those five isogenic isolates prolonged incubation period of X. oryzae pv. Oryzae, i.e. 6.6–5.4 days compared to the wild-type i.e. 4.8 days after inoculation. Suppression of disease progression was also observed with AUDPC value 615.0-827.5, which was lower than those caused by wild-type and control, i.e. 863.3 and 682.5, respectively. Application of isogenic strain isolates did not affect plant growth but it increased plant productivity. Isogenic strain M101 was the best isolate with an incubation period of 5.7 days after inoculation, having the lowest AUDPC value (615.0), and yielded dry weight of 100 grains higher (2.8 g) than those of the wild-type (2.0 g).

Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan Maryono, Tri; Widiastuti, Ani; Priyatmojo, Achmadi
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root and basal stem rot disease caused by Xylaria sp. is a new diseases in Indonesian sugarcane plantation. The disease has been reported only occurred in sugarcane plantations in Sumatera. Limited information about the disease has slowed down disease diagnosis in the field. The research was aimed to describe symptoms, signs, and spatial distribution of the disease caused by Xylaria. A survey was conducted at a sugarcane plantation in South Sumatera (3° 26’ 16.6” S dan 104°40’ 09.8” E). Typical symptoms of the disease involved drying of all leaves and plant death, dry rot of root and basal stem, and retarded growth of ratoon cane were recorded. Stroma was found on surface of diseased stems and on soil surface of diseased plants, and spatial aggregation of diseased plants was observed in all locations. The distribution of the diseased plant aggregately and the presence of stroma on diseased plants are important characteristics of the disease diagnosis in sugarcane plantations.

Pemodelan Keparahan Penyakit Blas pada Tanaman Padi di Kabupaten Subang Zulaika Zulaika; Bonny Poernomo Soekarno; Ali Nurmansyah
Jurnal Fitopatologi Indonesia Vol. 14 No. 2 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Modelling on Rice Blast Disease Severity in Subang DistrictBlast disease (Pyricularia oryzae) is a major diseases of rice in Indonesia. Research related to modelling of blast disease severity is limited. Therefore, this study aimed to design a statistical model on rice blast disease infestation on the rice paddy and to asses a correlation between the disease severity and infected seed level. The models were constructed based on multiple linier regression analyses. The study was conducted by observing the disease severity, collecting information about cultivation technique and weather conditions. The result of regression analysis showed severity modeling on influencing factors is Y = -67.17 + 5.51X1 – 10.54X2 + 13.26X3 + 8.51X4 + 2.29X5 + 1.32X6 + 8.47X7 + 0.31X8 + 4.53X9 (p-value <0.0001, R2 = 0.85). Nitrogen application and plant ages had significant effect on disease severity. The addition of N fertilizer increased the severity of blast disease by 8.47%. Increasing the daily life of plants increases the disease severity by 0.31%. The correlation (r) of pathogen infection after harvesting was strongly influenced by infection before planting with correlation value of 0.78. This means that the severity of seed disease after harvesting is influenced by 78% infection of pathogenic seed before planting. The result of regression analysis showed Y = 5.98 + 2.41X (p-value = 0.0076, R2 = 0.61). An increase of 1% pathogens carried by seed before planting will be increasing disease severity by 2.41% after harvesting. The results of this study can be used as a reference in preparing preventive control measure and reduce the risk of pathogen carried by seeds that act as a source of initial inoculum.

Seleksi Komposisi Medium Pertumbuhan dan Bahan Pembawa untuk Formulasi Cendawan Agens Hayati Fusarium oxysporum Non-Patogenik P21a Fany Juliarti Panjaitan; Suryo Wiyono; Rahayu Widyastuti
Jurnal Fitopatologi Indonesia Vol 15 No 2 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Selection of Compositions of Growth Medium and Carriers for Formulation of Biological Agents of Non-Pathogenic Fusarium oxysporum P21aThe potency of non-pathogenic Fusarium oxysporum P21a (NPFo P21a) as a biological control agent has prospect to be developed commercially. The growth medium and carrier are the critical factor to formulate biological control. This study aimed to obtain the best solid medium dan lighting type for mycelium growth and sporulation as well as to determine the carrier and storage temperature which is suitable for NPFo P21a toward the survival of propagule and germination of shallot. The results showed that M2 medium treatment (rice grain:rice bran; 20:1 w/w) was the best medium for mycelium growth. The M3 medium treatment (20:2 w/w) was the best medium for sporulation of NPFo P21a. The near-UV lighting treatment was able to stimulate the macroconidium production of NPFo P21a. Talc-based formulation and the storage at 20 °C showed the best shelf-life for NPFo P21a with density of viable propagule and shallot germination better.

Penggunaan Bakteri Kitinolitik sebagai Pengendali Hayati Colletotrichum capsici pada Tanaman Cabai Dian Syahfitri; Nisa Rachmania Mubarik; Lisdar A Manaf
Jurnal Fitopatologi Indonesia Vol 14 No 4 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Use of Chitinolytic Bacteria as Biological Control of Colletotrichum capsici on Chili PlantsColletotrichum capsici is known as the causal agent of anthracnose disease in chili plant and may cause reduction of crop yield. Chitinolytic bacteria, namely Serratia marcescens KAHN 15.12, Bacillus thuringiensis SAHA 12.12, and BAE 36 were reported to have antagonistic activity against C. capsici. Therefore, a study was conducted to determine the potential of chitinolytic bacteria on controlling C. capsici on chili plants in greenhouse experiment. Three bacterial isolates used as biocontrol agent was formulated by using talcum as carrier materials. The methodologies consisted of characterization of bacterial isolates, formulation of biocontrol agent, viability test of bacterial isolate, efficacy of biocontrol agents in the laboratory and in the greenhouse. Disease severity in the laboratory reached 64% when chili treated with isolate formulation of BAE 36. In the greenhouse, BAE 36 isolate formulation and consortium formulation were able to suppress infection of C. capsici; each was indicated by disease incidence of 25% and 50%, respectively. These results indicated that chitinolytic bacterial formulations could be potencial as biocontrol agents of C. capsici.

Cucumber mosaic virus pada Tanaman Lada di Yogyakarta dan Bangka Belitung Emerensiana - Uge; sri sulandari; sedyo - hartono; susamto - somowiyarjo
Jurnal Fitopatologi Indonesia Vol 15 No 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Cucumber mosaic virus on Black Pepper in Yogyakarta and Bangka BelitungPepper (Piper nigrum) is spice crop which has been cultivated a long time ago in Indonesia. Stunting is one of disease on pepper caused by cucumber mosaic virus (CMV). The research aimed to diagnose the biological, morphological and nucleaic acid characters of CMV on pepper in Yogyakarta and Bangka Belitung. CMV infection on pepper in both area (Putat dan Kleben village, Yogyakarta and Air Buluh village, Bangka Belitung) showed typical symptoms such as mosaic, narrow leaves and stunting. The disease incidence and disease severity of stunting disease are varies. The virus able to transmitted by cutting, grafting and mechanically on Nicotiana tabacum and Chenopodium amaranticolor. However, it was unable to transmitted mechanically on pepper and by Aphis gossypii. The virus particles were isometric with diameter size 28-30 nm. RT-PCR using coat protein partial gene primer successfully amplified a DNA with size ± 500 bp from all three samples. The homology of nucleotide between three isolates was 98-97%, while the highest homology of those three strains CMV from Yogyakarta and Bangka Belitung was 98% against strains from China in Brassica chinensis. Three strains CMV from pepper were in the same group, and separated from CMV pepper lines from Indonesia and other CMV isolates.

Identifikasi Patotipe Xanthomonas oryzae pv. oryzae dari Tanaman Padi di Sulawesi Selatan Asysyuura, Asysyuura; Nawangsih, Abdjad Asih; Mutaqin, Kikin Hamzah; Sudir, Sudir
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight (BLB), one of important constraint in rice production. The pathogen is known to have many pathotypes which caused difficulties in disease control. This research was conducted to determine the distribution of X. oryzae pv. oryzae’s pathotypes in seven districts in South Sulawesi. Grouping of pathotypes was performed according to Kozaka method, i.e. based on the response of differential rice varieties. Infection of X. oryzae pv. oryzae was confirmed by PCR using specific primers XOR-R2/XOR-F. Out of 36 isolates, 29 isolates were identified as X. oryzae pv. oryzae which belong to pathotype III (6 isolates), pathotype IV (21 isolates), and pathotype XII (2 isolates). This result indicated that X. oryzae pv. oryzae pathotype IV was distributed widely in South Sulawesi.

Deteksi dan Identifikasi Fitoplasma yang Berasosiasi dengan Penyakit Layu Kelapa Di Pulau Derawan, Kalimantan Timur Prasetyo, Agus Eko; Mutaqin, Kikin Hamzah; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Coconut is a major commodity in Derawan island as source of additional income for the farmers. Research was conducted to detect and identify phytoplasmas associated with coconut wilt disease in Derawan island. Coconut wilt disease was indicated by the typical symptoms, i.e. leaf yellowing, shorten of coconut sheaths and leaves, necrosis and collapse of old leaves, and also nut fall. The presence of phytoplasmas in phloem tissues of coconut stem was observed using fluorescence and electron microscope. Identification of phytoplasmas was carried out by nested-PCR and sequencing of the 16S rRNA gene. DNA fragment of phytoplasma with the size of 1.25 kbp was successfully amplified using primer pairs P1/P7, followed by primer pairs R16F2n/R16R2. Sequence analysis of the amplified fragments showed that phytoplasma associated with coconut wilt disease in Derawan island belongs to 16SrII (witches broom phytoplasma) and 16SrXI (ca. Phytoplasma oryzae) groups.

Page 17 of 42 | Total Record : 412

15 16 17 18 19

Filter by Year

2012 2025

Filter By Issues

All Issue Vol. 21 No. 1 (2025): Maret 2025 - IN PROGRESS Vol. 20 No. 6 (2024): November 2024 - IN PROGRESS Vol. 20 No. 5 (2024): September 2024 Vol. 20 No. 4 (2024): Juli 2024 Vol. 20 No. 3 (2024): Mei 2024 Vol. 20 No. 2 (2024): Maret 2024 Vol. 20 No. 1 (2024): Januari 2024 Vol 19 No 6 (2023): November 2023 Vol 19 No 5 (2023): September 2023 Vol 19 No 4 (2023): Juli 2023 Vol. 19 No. 4 (2023): Juli 2023 Vol 19 No 3 (2023): Mei 2023 Vol 19 No 2 (2023): Maret 2023 Vol. 19 No. 2 (2023): Maret 2023 Vol 19 No 1 (2023): Januari 2023 Vol. 18 No. 6 (2022): November 2022 Vol. 18 No. 5 (2022): September 2022 Vol. 18 No. 4 (2022): Juli 2022 Vol. 18 No. 3 (2022): Mei 2022 Vol. 18 No. 2 (2022): Maret 2022 Vol. 18 No. 1 (2022): Januari 2022 Vol 17 No 6 (2021) Vol 17 No 5 (2021) Vol 17 No 4 (2021) Vol 17 No 3 (2021) Vol 17 No 2 (2021) Vol 17 No 1 (2021) Vol 16 No 6 (2020) Vol. 16 No. 5 (2020) Vol 16 No 4 (2020) Vol. 16 No. 3 (2020) Vol 16 No 2 (2020) Vol 16 No 1 (2020) Vol 15 No 6 (2019) Vol 15 No 2 (2019) Vol 15 No 1 (2019) Vol 14 No 6 (2018) Vol 14 No 5 (2018) Vol 14 No 4 (2018) Vol. 14 No. 3 (2018) Vol. 14 No. 2 (2018) Vol 14 No 1 (2018) Vol. 14 No. 1 (2018) Vol. 13 No. 6 (2017) Vol 13 No 5 (2017) Vol. 13 No. 5 (2017) Vol 13 No 4 (2017) Vol. 13 No. 3 (2017) Vol. 13 No. 2 (2017) Vol. 13 No. 1 (2017) Vol 12 No 6 (2016) Vol 12 No 5 (2016) Vol 12 No 4 (2016) Vol 12 No 3 (2016) Vol 12 No 2 (2016) Vol 12 No 1 (2016) Vol 11 No 6 (2015) Vol 11 No 5 (2015) Vol 11 No 4 (2015) Vol 11 No 3 (2015) Vol 11 No 2 (2015) Vol 11 No 1 (2015) Vol 10 No 6 (2014) Vol 10 No 5 (2014) Vol 10 No 4 (2014) Vol 10 No 3 (2014) Vol 10 No 2 (2014) Vol 10 No 1 (2014) Vol 9 No 6 (2013) Vol 9 No 5 (2013) Vol 9 No 4 (2013) Vol 9 No 3 (2013) Vol 9 No 2 (2013) Vol 9 No 1 (2013) Vol 8 No 6 (2012) Vol 8 No 5 (2012) Vol 8 No 4 (2012) Vol. 8 No. 3 (2012) Vol. 8 No. 2 (2012) Vol. 8 No. 1 (2012) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA