Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Jurnal Fitopatologi Indonesia

jfiti
Website

Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

Arjuna Subject : -

Articles 412 Documents

14 15 16 17 18

Waktu Inkubasi pada Derajat Distilasi Kitosan Enzim dan Efektifitas Penghambatannya terhadap Penyakit Antraknosa Yadi Suryadi; Tri Puji Priyatno; I Made Samudra; Dwi Ningsih Susilowati; Hermawati Nurzulaika; Syaefudin Syaefudin
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The use of chitosan as a coating agent of harvested fruits is an alternative method in controlling anthracnose disease (Colletotrichum sp.). This study aimed to obtain an optimal enzymatic chitosan (EC) that hydrolyzed using chitinase from Burkholderia cepacia isolate E76. The optimal incubation condition to produce EC was 2 h with the yield of 3.52 ± 0.38 g. The degree of deacetylation (DD) chitosan and EC was 66.91% and 80.91%, respectively. Based on in vitro assays, EC 2% was the most effective in inhibiting the growth of Colletotrichum sp. (94.22%) than chitosan, while the highest inhibition for chitosan 3% was 55.26%. Moreover, the EC 2% showed the highest inhibition of spore germination (74.12%). The in vivo assay revealed that EC 2% showed the highest inhibition on the fungal growth (88.88%), compared to the other concentrations. On the other hand, the EC 2% and 3% gave similar results on inhibition of Colletotrichum sp.of chili (55.55%).

Dampak Penyakit Tanaman terhadap Pendapatan Petani Kubis-kubisan di Daerah Agropolitan Kabupaten Cianjur, Jawa Barat Teguh Pratama; Gede Suastika; Ali Nurmansyah
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Cruciferous vegetables are very important agricultural commodities for increasing farmers income. The main obstacles in their cultivation involved among others high level of pathogen infestation that may cause reduction in farmer’s income. This research was conducted to determine the main pathogens and their impact on farmer income in Agropolitan area of Cianjur Regency, West Java Province. The research was conducted through three activities, i.e. farmer survey, identification of the main pathogens, and measurement of disease intensity. The results showed that there were three main pathogens on assessed cruciferous vegetables, i.e. Alternaria brassiccicola (alternaria leaf spot), Plasmodiophora brassicae (clubroot), and Xanthomonas campestris (black rot), with disease intensity of 16.7%, 18.7%, and 15.1%, respectively. Clubroot disease was the most affecting disease in decreasing the production of cruciferous vegetables and the farmer income. Incidence of clubroot disease with an average disease intensity of 16.67% might lead to the decrease of farming income about 24%–28%.

Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa Umi Kulsum; Sedyo Hartono; Sri Sulandari; Susamto Sumowiyarjo
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Soybean (Glycine max) is an important food crops in Indonesia, but the production is considered low. Infection of Cowpea mild mottle virus (CPMMV) may affect to the productivity of the crops. The virus cause a variety of symptoms on soybean such as mosaic, chlorosis, necrosis, distortion, and leaf dwarf. The aims of this study were to detect and identify CPMMV by molecular technique from some varieties of soybeans, i.e. ‘Anjasmoro’, ‘Argomulyo’, ‘Grobogan’, and two local Edamame varieties. Symptomatic samples was obtained randomly from main soybean growing areas in Java. DNA amplification using specific primer for coat protein of CPMMV was only successfully gained from leaf samples of var. Argomulyo from Malang which showing mosaic on the leaf surface, vein clearing, and chlorosis. Further amino acid and nucleotide sequence analysis showed that this CPMMV isolate has highest homology (99%) to CPMMV isolate of French bean from China.

Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat Irsan Nuhantoro; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya. The disease had not been found in Indonesia, until disease outbreak in Nangroe Aceh Darussalam was reported in 2012. Since then, the disease spread rapidly in most papaya growing areas in Sumatera, Java and Bali. Papaya ringspot virus (PRSV) is generally detected using serological or polymerase chain reaction methods. Improvement in detection method is necessary to facilitate a more reliable tool for controlling the spread of PRSV. The aim of the research was to construct DNA probe for development of detection method based on nucleic acid hybridization. Molecular characterization based on HCPro gene sequence indicated high homology (97.88 to 99.05%) among PRSV isolates from Boyolali (Central Java), Medan (North Sumatera), Sleman (Yogyakarta) and Tabanan (Bali). Two DNA clones of HCPro gene were selected for probe construction and the probes were then labeled using PCR DIG-dioxigenin. Optimization of nucleic acid dot blot hybridization method to achieve strongest positive reaction was developed, i.e. using stringency washes at 1×SSC, 0.1% SDS, incubation at 60 oC for 15’. The DNA probe for PRSV has a high specificity and sensitifity; it could detect PRSV at the lowest concentration of nucleic acid (0.062 µg µL-1).

Deteksi Odontoglossum ringspot virus pada Anggrek Asli Koleksi Kebun Raya di Indonesia Mahfut, Mahfut; Daryono, Budi Setiadi; Somowiyarjo, Susamto
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Native orchid is one of Indonesian natural resources which play important role as parental materials in breeding program. Virus infection is one of the limiting factors in the cultivation of orchid. The purpose of this study was to detect Odontoglossum ringspot virus (ORSV) from native orchid. Symptomatic orchids were collected from 5 botanical gardens, i.e. Bogor, Cibodas, Purwodadi, Balikpapan, and Enrekang Botanical Gardens. Detection and identification was conducted by serological method using ORSV specific antisera, followed by RT-PCR and DNA sequencing. The serological test showed that 5 samples gave positive reaction against ORSV antiserum, i.e. Phalaenopsis amboinensis (KRB2) and Phalaenopsis amabilis (KRB12) from Bogor Botanical Garden, Phalaenopsis amabilis (KRP18) and Dendrobium salacence (KRP20) from Purwodadi Botanical Garden, dan Phalaenopsis modesta J. J. Sm. (KRBp5) from Balikpapan Botanical Garden. RT-PCR of the 5 samples using specific primer of ORSV coat protein gene was successfully amplified fragment DNA with size ± 474 bp. Homology analysis of those 5 ORSV isolates showed the highest index similiarity of 99.8% with corresponding sequences from 14 other ORSV isolates. Phylogenetic analysis indicated that ORSV KRB2 and KRP18 isolates was clustered in a separate group far from ORSV isolates in other countries. This is the first report of ORSV infection on native orchids collection from 5 botanical gardens in Indonesia.

Keragaman Morfologi, Genetika, dan Patogenisitas Colletotrichum acutatum PenyebabAntraknosa Cabai di Jawa dan Sumatera Ibrahim, Roy; Hidayat, Sri Hendrastuti; widodo, widodo
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Colletotrichum acutatum is known as the major causal agents of anthracnose disease of chilli pepper in Indonesia. This research was aimed to study genetic variability of C. acutatum in Java and Sumatera based on morphological and molecular characteristics and to evaluate pathogenicity of several isolates. Observation on morphological characteristics involved colour and growth of each colony as well as shape and size of conidia. Molecular identification was performed by DNA amplification using specific primer for C. acutatum, i.e. CaInt2/ITS4 followed by sequencing and nucleotide sequence analysis. Pathogenicity test for each isolate on Capsicum annuum and C. frutescens was conducted in the laboratory using detached chilli. Morphology variability was observed from 40 isolates of C. acutatum. Most of the isolates have white/beige colonies with fusiform conidia of 6.11–9.73 µm in length and 2.24–2.73 µm in width. Pathogenicity of 3 C. acutatum isolates varies from moderate to high based on lesions size on infected chilli. Sequence analysis of 8 C. acutatum isolates indicated high homology among isolates from Java and Sumatera

Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya Khairani, Hagia Sophia; Sinaga, Meity Suradji; Mutaqin, Kikin Hamzah
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant diseases become an important constraint on citrus production. Stem rot disease caused by Botryodiplodia theobromae is a major disease on citrus in Indonesia. This study was aimed to evaluate the mechanism of yeast, arbuscular mychorrhiza fungi (AMF), symbiotic bacteria of AMF and chitosan in controlling stem rot disease. In vitro study was performed to evaluate the mechanism of antibiosis, hyperparasitism, production of volatile compounds, and production of chitinase enzyme. The experiment was continued by in planta assays using yeast, AMF, symbiotic bacteria of AMF, and chitosan either singly or in combination. The experiments were performed using completely randomized design with 3 replications. Disease progress were observed based on its latent period, AUDPC, infection rate, AMF association rate, and total phenol content. In vitro studies indicated that the yeasts showed hyperparasitism to B. theobromae with affinity of 26 cells per hyphae, produced volatile compounds with relative resistance level (RRL) 29.1%, and produced chitinase. Selected symbiotic bacteria from AMF showed antibiosis with RRL 42.9%, production of volatile compounds with RRL 26.7%, and has 98% homology with Bacillus subtilis from Vietnam. Application of yeast + AMF + chitosan delayed disease latent period and suppressed disease incidence. Single AMF and combination of yeast + AMF suppressed disease severity, infection rate, and AUDPC. Application of yeast + AMF showed highest association level of AMF and total phenol content. Therefore, the application of AMF and yeast + AMF is recommended in controlling citrus stem rot disease.

Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat Kurniawati, Fitrianingrum; Supramana, Supramana; Adnan, Abdul Muin
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root knot nematodes (RKN) Meloidogyne spp. is the primary parasite of celery and reported to cause losses up to 70%. Identification of the Meloidogyne species on celery in Indonesia has not been reported. This study was aimed to identify the species of Meloidogyne on celery based on morphology characters. Samples of Meloidogyne-infected celery plants were taken using purposive sampling method from Ciputri Village, District of Pacet, Cianjur, West Java Province. RKN inside root tissue was detected by acid fuchsin staining method. Meloidogyne species identification was done by morphological observation of female perineal pattern. Disease symptoms found in the field include leaf yellowing, stunted, and uneven growth of celery plants. Roots of infected plants showed the formation of small size root knots, in large numbers and forming strands like a chain. Staining NPA in root tissue was successfully detected various stages of nematode development, i.e. eggs, juveniles and female nematodes. Three Meloidogyne species, namely M. incognita, M. arenaria and M. javanica were identified.

Penyakit Karat Bawang Daun yang Disebabkan oleh Puccinia allii Rud. Widodo, Widodo; Pamungkas, Ginting Tri; Susetyo, Hendry; Setiawan, Antoni; Wowor, Johanis
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

New disease of bunching onion (Allium fistulosum) with typical symptoms as rust disease was found in the sub-district of West Tompaso and Modoinding, District of South Minahasa, North Sulawesi in June 2016. Infected plants in the field showed yellowish brown pustules on leaf surface, followed by leaf yellowing and drying out started from the tip. Disease incidence in surveyed area reached 100% with disease severity between 30%–50%. Based on microscopic observation of the fungal morphological characteristic, i.e. uredium dan urediniospora, the causal agent was identified as Puccinia allii Rud.

Kepekaan Erythricium salmonicolor Penyebab Penyakit Jamur Upas pada Kemiri Sunan terhadap Suhu, Cahaya, dan Fungisida Florina, Dini; Wahyuno, Dono; Siswanto, Siswanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

A typical pink disease symptom appeared on stem of Reutealis trisperma in Bogor. The present study was aimed for confirming the causal pathogen and testing its sensitivity to temperature, light and fungicides. The fungus was identified based on its morphological characteristics. The fungal inoculum was obtained by tissue isolation of infected stem bark. For pathogenicity test, the fungus was inoculated onto sterilized pieces of woody R. trisperma branch, then fastened on the seedlings and incubated in the green house. The effect of temperatures on fungal growth was tested by planting the fungal colony onto potato dextrose agar (PDA) then incubated at 25–34 °C. The sensitivity of the fungus to light was tested by inoculating the fungus into flask with potato dextrose broth (PDB) medium on a shaker (60 rpm) exposing it to white light tube (400 luks) or in a dark condition for 15 days at 25 °C. The obtained fungal colonies were weighed. Efficacy of copper hydroxide and mancozeb against the fungal colony growth was tested by inoculating the fungus onto PDA amended with 100, 200, 300, 400, and 500 ppm, respectively, of tested fungicides. The results showed that the causal pathogen was Erythricium salmonicolor. In the pathogenicity test, cobwebs of E. salmonicolor were formed on inoculated seedlings 3 weeks after inoculation. The optimum temperature for growth of E. salmonicolor was 28 °C. There was no significant effect of light on fungal growth. Mancozeb suppressed colony of E. salmonicolor growth better than copper hydroxide.

Page 16 of 42 | Total Record : 412

14 15 16 17 18

Filter by Year

2012 2025

Filter By Issues

All Issue Vol. 21 No. 1 (2025): Maret 2025 - IN PROGRESS Vol. 20 No. 6 (2024): November 2024 - IN PROGRESS Vol. 20 No. 5 (2024): September 2024 Vol. 20 No. 4 (2024): Juli 2024 Vol. 20 No. 3 (2024): Mei 2024 Vol. 20 No. 2 (2024): Maret 2024 Vol. 20 No. 1 (2024): Januari 2024 Vol 19 No 6 (2023): November 2023 Vol 19 No 5 (2023): September 2023 Vol 19 No 4 (2023): Juli 2023 Vol. 19 No. 4 (2023): Juli 2023 Vol 19 No 3 (2023): Mei 2023 Vol 19 No 2 (2023): Maret 2023 Vol. 19 No. 2 (2023): Maret 2023 Vol 19 No 1 (2023): Januari 2023 Vol. 18 No. 6 (2022): November 2022 Vol. 18 No. 5 (2022): September 2022 Vol. 18 No. 4 (2022): Juli 2022 Vol. 18 No. 3 (2022): Mei 2022 Vol. 18 No. 2 (2022): Maret 2022 Vol. 18 No. 1 (2022): Januari 2022 Vol 17 No 6 (2021) Vol 17 No 5 (2021) Vol 17 No 4 (2021) Vol 17 No 3 (2021) Vol 17 No 2 (2021) Vol 17 No 1 (2021) Vol 16 No 6 (2020) Vol. 16 No. 5 (2020) Vol 16 No 4 (2020) Vol. 16 No. 3 (2020) Vol 16 No 2 (2020) Vol 16 No 1 (2020) Vol 15 No 6 (2019) Vol 15 No 2 (2019) Vol 15 No 1 (2019) Vol 14 No 6 (2018) Vol 14 No 5 (2018) Vol 14 No 4 (2018) Vol. 14 No. 3 (2018) Vol. 14 No. 2 (2018) Vol. 14 No. 1 (2018) Vol 14 No 1 (2018) Vol. 13 No. 6 (2017) Vol 13 No 5 (2017) Vol. 13 No. 5 (2017) Vol 13 No 4 (2017) Vol. 13 No. 3 (2017) Vol. 13 No. 2 (2017) Vol. 13 No. 1 (2017) Vol 12 No 6 (2016) Vol 12 No 5 (2016) Vol 12 No 4 (2016) Vol 12 No 3 (2016) Vol 12 No 2 (2016) Vol 12 No 1 (2016) Vol 11 No 6 (2015) Vol 11 No 5 (2015) Vol 11 No 4 (2015) Vol 11 No 3 (2015) Vol 11 No 2 (2015) Vol 11 No 1 (2015) Vol 10 No 6 (2014) Vol 10 No 5 (2014) Vol 10 No 4 (2014) Vol 10 No 3 (2014) Vol 10 No 2 (2014) Vol 10 No 1 (2014) Vol 9 No 6 (2013) Vol 9 No 5 (2013) Vol 9 No 4 (2013) Vol 9 No 3 (2013) Vol 9 No 2 (2013) Vol 9 No 1 (2013) Vol 8 No 6 (2012) Vol 8 No 5 (2012) Vol 8 No 4 (2012) Vol. 8 No. 3 (2012) Vol. 8 No. 2 (2012) Vol. 8 No. 1 (2012) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA