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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 417 Documents

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Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara Utami, Budi Sri; Supramana, Supramana; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root-knot nematode (Meloidogyne spp.). is one of the main constraint of potato production in North Sulawesi. Little is known about Meloidogyne species infecting potatoes in North Sulawesi. Therefore, research was conducted to identify Meloidogyne spp. on potatoes in North Sulawesi and further study their relationship with related species from other countries. Infected potato tubers with pimple-like knot symptom were collected from three potato production centers, i.e. Kakenturan (South Minahasa), Purworejo (East Bolaang Mongondow) and Singsingon (…?? Bolaang Mongondow). Morphological identification was conducted based on the perineal pattern of the female; whereas molecular identification was conducted by PCR using specific primer for ITS-rDNA, followed by DNA sequencing and phylogenetic analysis. Two Meloidogyne species were identified i.e. M. javanica (samples form Kakenturan, Purworejo and Singsingon) and M. incognita (samples from Purworejo). M. javanica and M. incognita from North Sulawesi are similar to the related species from China with homology level of 97.5 % and 100 %, respectively.

Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro Wibowo, Risky Hadi; Mubarik, Nisa Rachmania; Rusmana, Iman; Thenawidjaya, Maggy
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Chitinolytic bacteria have been reported as biocontrol agents and have the ability to produce chitinase enzymes. The objective of the research was to obtain chitinase producing bacteria that had antagonistic activity to Ganoderma boninense, a causal agent of basal stem rot on oil palm. A total of 63 isolates of chitinase producing bacteria were isolated from soil of Bukit Dua Belas National Park and oil palm plantation in Jambi Province; all was screened for their potency in inhibiting G. boninense in vitro. Three isolates designated TB04-05, SW01-11, and SW02-08 were potentially suppressed and inhibited the mycelium growth of G. boninense in vitro. Based on their specific chitinase activity, these three isolates produced the highest level of chitinase enzyme of 6.3072 U mg-1 protein, 6.0385 U mg-1 protein and 6.1279 U mg-1 protein, respectively after 24 hr incubation. Based on 16S RNA identification, strain TB04-05 had similarity with Bacillus cereus, whereas strains SW01 and SW02-08 had similarity with Bacillus thuringiensis.

Kemampuan Campuran Bacillus sp., Pseudomonas fluorescens, dan Trichoderma sp. untuk Mengendalikan Penyakit Layu Bakteri pada Tanaman Tomat abdul manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Fitopatologi Indonesia Vol. 14 No. 2 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Ability of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. to Control Tomato Bacterial Wilt DiseaseRalstonia solanacearum is known as the causal agent of bacterial wilt on tomato. The bacteria may infect all stadia of plant growth and decrease tomato production. Biological control using antagonistic microbes is considered as a potential control alternative for the disease. This research was aimed to assay the ability of combination treatment of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. in controlling R. solanacearum and its effect on growth and yield of tomato in the field. The treatments consisted of control (without antagonistic microbes), mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp.; Bacillus sp. B8 + Pseudomonas flourescens P8 + Trichoderma sp.; and Streptomisin sulfat 20%. Antagonistic microbes was applied at planting time, as much as 100 mL per plant. The result showed that mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp. was the best treatment in controlling the disease as indicated by delaying incubation period up to 6.2 days, decreasing disease incidence up to 12.3%, increasing plant growth up to 42.80%, and increasing yield up to 14.99%.

Efektivitas Formula Bacillus subtilis TM4 untuk Pengendalian Penyakit pada Tanaman Jagung Nurasiah Djaenuddin; Nurnina Nonci; Amran Muis
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Banded leaf and sheath blight (BLSB) and maydis leaf blight (MLB) caused by Rhizoctonia solani and Bipolaris maydis, respectively are considered as important diseases in maize. The use of biopesticides is an alternative method to control the diseases. This study was conducted to determine the effectiveness of bacterial formula Bacillus subtilis to inhibit the development of BLSB and MLB on the plant. Testing of biopesticide formula was done in two different applications, i.e. seed treatment for BLSB control and leaf spraying in the field for MLB. The results showed that the B.subtilis formula effectively suppressed the development of BLSB but it was not effectively suppressed the development of MLB .Key words: Bacillus subtilis, biopesticide, Bipolaris maydis, leaf blight diseaseBanded leaf and sheath blight (BLSB) and maydis leaf blight (MLB) caused by Rhizoctonia solani and Bipolaris maydis, respectively are considered as important diseases in maize. The use of biopesticides is an alternative method to control the diseases. This study was conducted to determine the effectiveness of bacterial formula Bacillus subtilis to inhibit the development of BLSB and MLB on the plant. Testing of biopesticide formula was done in two different applications, i.e. seed treatment for BLSB control and leaf spraying in the field for MLB. The results showed that the B.subtilis formula effectively suppressed the development of BLSB but it was not effectively suppressed the development of MLB.

Distribusi Nematoda Pucuk Putih Padi Aphelenchoides besseyi di Pulau Jawa Didiet Rahayu Diana
Jurnal Fitopatologi Indonesia Vol 14 No 4 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Distribution of Rice White Tip Nematode Aphelenchoides besseyi in Java Island Aphelenchoides besseyi is one of the most important rice seed borne nematode causing white tip disease in Indonesia. The status of A. besseyi is considered as quarantine pest category A2, with limited distribution area in Java, Sumatera, and South Kalimantan). The aim of this research was to detect and identify A. besseyi from rice seed based on morphological, morphometry and molecular approaches and to determine its distribution in rice growing areas in Java. Rice seed samples, consisting of 26 varieties, were obtained from seed producers, seed distributors, and farmers in Java. Nematode extraction was done using Baermann method. Molecular identification was carried out by polymerase chain reaction and followed by DNA sequencing and nucleotide analysis. A. besseyi was detected from 74.32% of seed samples. A. besseyi was confirmed to be distributed in Lebak, Bogor, Sukabumi, Subang, Indramayu, Klaten, Sragen, Sukoharjo, Boyolali, Pati, Pemalang, Yogyakarta Sleman, Magetan, Blitar, Tuban, Gresik, Nganjuk, and Banyuwangi. Further nucleotide analysis showed that A. besseyi isolates from rice seed cv Ciherang (Yogyakarta) and cv IR 64 (Banyuwangi) have 99% homology to those from India, China, USA; and 98% homology to those from Taiwan.

Sensitivitas Colletotrichum spp. pada Cabai terhadap Benomil, Klorotalonil, Mankozeb, dan Propineb Desta Andriani; Suryo Wiyono; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Anthracnose caused by several species of Colletotrichum is one of limiting factors for chilli production. Up to now the control of antrachnose disease still rely on regular and intensive application of synthetic fungicides. Application of similar active ingredients of synthetic fungicides may create resistance of the pathogens. This study was conducted to evaluate the sensitivity of three species of Colletotrichum against four commercial fungicides with different active ingredients, i.e. benomyl, chlorotalonil, mancozeb, and propineb. The sensitivity level and the potential occurrence of resistance of Colletotrichum were observed based on relative inhibition rate using poisoning food method. The results showed that all isolates were highly resistant to chlorotalonil, even up to ten times of the highest recommended concentrations. The tested fungal isolates were resistant or highly resistant to the recommended concentrations of mancozeb and propineb, but they were still sensitive when the concentrations were increased up to five times. Isolates of Colletotrichum spp. showed sensitive to highly sensitive response to benomyl.

Penanda Molekuler Inter Simple Sequence Repeat untuk Menentukan Ketahanan Tanaman Jagung terhadap Penyakit Bulai Polikarpia Wilhelmina Bani; Budi Setiadi Daryono; Purnomo Purnomo
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Downy mildew disease of maize, caused by the fungus Peronosclerospora maydis, can cause serious yield loss. Molecular markers-based method is usually performed to look for the variation of resistance in plants. In this study, 5 primers of inter simple sequence repeat (ISSR) was evaluated for molecular marker to determine maize resistance to downy mildew. The average polymorphic of 5 ISSR primers was 72.84%. Primer ISSR 808 (AG) 8C was able to differentiate resistant cultivar (cv Talenta) from susceptible cultivar (cv Pulut).

Keefektifan Perlakuan Fisik dan Minyak Atsiri untuk Mengeliminasi Bakteri Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Siti Tri Wahyuni; Ali Nurmansyah; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Clavibacter michiganensis subsp. michiganensis is the causal agent of bacterial canker disease of tomato. The bacteria is seed borne and may cause yield loss up to 70%. Seed treatment is an alternative method for controlling bacterial canker. The objective of the research was to study the effectiveness of physical and essential oil treatment for elimination of C. michiganensis subsp. michiganensis from tomato seed. Research was conducted in 4 separate experiments, i.e. (1) screening essential oils to control C. michiganensis subsp. Michiganensis; (2) to determine the treatment window of physical and essential oil treatment; (3) to determine the physical and essential oil treatment on tomato seed infested by C. michiganensis subsp. Michiganensis; and (4) to determine the effectiveness of treatment combination of physical and essential oil. The results showed that betel oil at concentration of 8% had the greatest inhibitory level (approximately 27.33 mm). Hot water treatment (53 °C) for 25 minutes reduced 90.94% of C. michiganensis subsp. michiganensis population; whereas dry heat treatment (60 °C, 24 hours) and betel oil treatment at 0.25% concentration reduced C. michiganensis subsp. michiganensis population (85.13 and 99.82% respectively). The combination of betel oil (0.5%), hot water (55 °C), and dry heat treatments (60 °C) was the most effective control method, because it reduced 99.99% of C. michiganensis subsp. michiganensis population and maintained the germination level of seed up to 100%. This combination might be recommended for seed treatment to eliminate C. michiganensis subsp. michiganensis.

Identifikasi Molekuler Fitoplasma yang Berasosiasi dengan Tanaman Kaktus Hias Opuntia sp. Ariny Prasetya; Kikin Hamzah Mutaqin; Meity Suradji Sinaga; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Cactus species (Opuntia sp.) is a popular ornamental succulent plant. Some ornamental cactus species in Indonesia showing proliferation and green mosaic pattern symptoms have been reported to be associated with phytoplasma infection. However, further molecular identification for accurate classification of the causal phytoplasma has not been done. This study aimed to diagnose phytoplasma associated with Opuntia sp. based on molecular methods involving PCR standard combined with nested-PCR, cloning and DNA sequencing. Standard PCR was carried out using P1/P7 primers followed by nested-PCR using R16F2n/R16R2 or fU5/rU3 primer pairs which amplify the 16S rRNA gene targets of 1.2 kb and 880 bp, respectively. Amplified fragment of nested-PCR using R16F2n/R16R2 primers was chosen to be cloned and sequenced for further identification and classification of phytoplasma. BLASTn analysis showed that the phytoplasma from Opuntia sp. was closely related to 16SrII group. Phylogenetic analysis and in silico RFLP indicated that phytoplasma strain infecting Opuntia sp. was a member of subgroup 16SrII-C (cactus witches’ broom phytoplasma). This is a newly report of cactus witches’ broom phytoplasma on Opuntia sp. in Indonesia.

Potensi Metabolit Sekunder Asal Bakteri Endofit dalam Menekan Pertumbuhan Miselium Ganoderma boninense Fitri Widiantini; Ceppy Nasahi; Endah Yulia; Serly Noviyawati
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Potency of Endophytic Bacterial Secondary Metabolite to Inhibit Mycelium Growth of Ganoderma boninense Endophytic bacteria have a close relationship with their host plants. The bacteria are living inside their host without causing any symptom. Endophytic bacteria are well known for their ability to produce compounds that can be used to protect plants from deleterious microorganisms. The research was conducted to determine the ability of endophytic bacteria in producing antifungal compounds that are able to suppress the growth of Ganoderma boninense, a causal agent of basal stem rot disease of oil palm. Previously isolated endophytic bacteria were grown on international streptomyces project 2 (ISP2) liquid medium and incubated at rotary evaporator at 120 rpm for 10 days. The supernatant was separated from bacterial cells and tested against G. boninense using well diffusion agar method. The results showed that endophytic bacteria isolate of BEK6, BEK7, BEK9 and BEK10 were able to produce antifungal compound against G. boninense. Microscopic observation demonstrated G. boninense mycelia alteration following secondary metabolite application.

Page 18 of 42 | Total Record : 417

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