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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 412 Documents

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Pengendalian Penyakit Busuk Pangkal Batang Kelapa Sawit secara Kultur Teknis Hari Priwiratama; Agus Eko Prasetyo; Agus Susanto
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Effort to manage basal stem rot disease (BSR) caused by Ganoderma boninense could be conducted through several cultural practice approaches. Four series of field experiments was conducted to observe the effectiveness of cultural practice methods for controlling the epidemic of basal stem rot due to G. boninense. Sanitation trial consisted of three treatments i.e root sanitation with big hole planting system, big hole without root sanitation, and standard replanting system as comparison. Hole in hole and standard planting system were compared in the hole in hole trial. Digging and mounding trial was conducted in four treatments, i.e. digging and mounding with teer and Trichoderma, digging and mounding with Trichoderma, mounding with Trichoderma, and infected palm without any treatment as comparison. Meanwhile, a 4 m × 4 m trench was used in the isolation trench trial. Annual observation was conducted to determine the disease incidence of BSR in each trial. Results showed that all methods could prevent palms from G. boninense infection in the early stage of oil palm development. Proper root sanitation delayed G. boninense infection until 2 years after treatment (YAT), hole in hole planting system was able to suppress disease incidence up to 7 YAT, digging and mounding was useful to prolong the life of the infected palms up to 3 YAT, meanwhile the use of isolation trench was demonstrated to prevent G. boninense infection to neighboring palms up to 2 YAT.

Pengelolaan Penyakit Kuning pada Tanaman Lada oleh Petani di Wilayah Bangka Abdul Munif; Ita Sulistiawati
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow disease caused by plant-parasitic nematodes are still a major problem on pepper production area in Bangka region. Significant yield losses occurred due to yellow disease. The objective of this research was to survey the yellow disease incidence and to study farmer’s knowledge, attitude and practices on the management of yellow disease of black pepper in Bangka district, Central Bangka and South Bangka. This study consisted of a survey of the incidence of yellow disease in the field and interviews to the farmers. The result showed that the yellow disease had infected nearly all of black pepper fields in the areas with incidence of as much as 20–60%. Almost all of the respondents reported that the yellow disease was the major disease of black pepper in Bangka region. Farmers’ level of knowledge about the causal of disease, symptoms, ecology and control methods of the yellow disease are still very limited. Various control measures have been done by farmers to combat the disease, especially by removing and burning the infected plants or by application of lime. A few farmers did not even controlling the disease. Pesticides have been used by a few farmers to control the yellow disease, but the application of pesticide does not in line with integrated pest management principles.

Sensitivitas Metode Serologi dan Polymerase Chain Reaction untuk Mendeteksi Bean Common Mosaic Potyvirus pada Kacang Panjang Sherli Anggraini; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Mosaic disease in yard long bean is caused by Bean common mosaic potyvirus (BCMV) and has been reported to affect yield. Common method to detect infection of BCMV involves serological assay and polymerase chain reaction (PCR). The aims of this research is to assess the sensitivity of three methods, i.e. Indirect Enzym-Linked Immunosorbent Assay (I-ELISA), Dot Immunobinding Assay (DIBA), and reverse transcription (RT)-PCR as detection method for BCMV infection in yard long bean. Sensitivity level of the methods was evaluated by diluting plant extract and antisera for I-ELISA and DIBA, and cDNA as template in RT-PCR. Virus isolate from Cirebon was maintained in yard long bean in screenhouse and used for the assessment. Absorbance value of ELISA showed that dilution end point for I-ELISA was reached at 10-3 and 10-2 of plant extract and antisera dilution, respectively. Positive infection was still detected using DIBA when the plant extract was diluted up to 10-5 based on development of color intensity on nitrocellulose membrane. Specific viral DNA fragment was still amplified when cDNA was diluted up to 10-4, indicated higher sensitivity level of RT-PCR method.

Penapisan dan Identifikasi Bakteri Agens Biokontrol Penyakit Layu Fusarium Hasil Isolasi dari Rizosfer Pisang Dwi Agustiyani; Achirul Nditasari; Nur Laili; Sarjiya Antonius
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Banana wilt disease caused by Fusarium oxysporum f.sp.cubense is considered as one of the most destructive diseases on banana plants in the tropical region. Biological control agents (BCA’s) have become a promising solution to overcome this disease. The objective of this study was to find potential BCA’s for wilt disease of banana plants. Bacteria and actinomycetes were isolated from banana’s rhizosphere in Lampung and Cianjur. As much as 64 actinomycetes and 142 bacteria isolates were obtained. Antagonistic test against F. oxysporum f. sp. cubense of those isolates showed that 21 bacteria and 10 actinomycete isolates have abilities to inhibit F. oxysporum f. sp. cubense. Actinomycetes showed relatively higher inhibition against F. oxysporum f. sp. cubense compared to bacteria. Isolates which have positive antagonistic activities against F. oxysporum f. sp. cubense were then tested for their protease, chitinase, and selulase activities qualitatively on specific medium. All actinomycetes which were tested had chitinase enzyme activities, while only 5 bacterial isolates had chitinase activities. On the other hand, 13 bacterial isolates showed protease activities and only 1 actinomycete showed protease activity. Two bacterial isolates (L.II.4.ND and L.A.I-5.DW) and 3 actinomycetes (L.A.I.DW, L.3.1.DW and Ci.I.A5.DW) which showed high inhibition against F. oxysporum f. sp. cubense and lyses enzymes activities were identified based on 16S rRNA genes. Analysis based on GenBank data, those isolates have 99% homology to Klebsiella pneumonia (L.II.4.ND), Burkholderia sp. (L.A.I-5.DW), Streptomyces sp. (L.A.I.DW), Streptomyces sp. (L.3.1.DW) and Streptomyces sp. (Ci.I.A5.DW).

Trichoderma spp. dan Penicillium spp. dari Tanah Rizosfer Lahan Rawa Lebak dalam Menginduksi Ketahanan Tanaman Cabai Terhadap Serangan Penyakit Rebah Kecambah Ahmad Muslim; Syahri Syahri; Harman Hamidson; Abdullah Salim
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Soil microbes associated with rhizosphere are important for promoting plant growth and inducing resistance to diseases. The research was conducted to study the ability of Trichoderma spp. and Penicillium spp. isolated from rhizosphere in lowland swampy area for controlling damping-off disease caused by Rhizoctonia solani Khun. Trichoderma spp. and Penicillium spp. were cultured in bran, corn meal, and rice straw containing media and applied as inoculum to 2-weeks old seedlings. Application of two fungi isolates effectively induced resistance of chili plants to damping-off disease. Trichoderma spp. and Penicillium spp. were significantly reduced disease incidence by 61.5–100% to 46.2–100%, respectively and disease severity by 50–100% and 30–95.9%, respectively. This experiment showed the potential of Trichoderma spp. and Penicillium spp. as biocontrol agents to control damping-off disease on chili.

Aplikasi Formula Campuran Rizobakteri untuk Pengendalian Penyakit Busuk Akar Rhizoctonia dan Peningkatan Hasil Kedelai di Tanah Ultisol Andi Khaeruni; Asniah Asniah; Muhammad Taufik; Gusti Ayu Kade Sutariati Ayu Kade Sutariati
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Rhizoctonia root rot disease caused by Rhizoctonia solani is one of the most important disease in soybean area, including at ultisol land in Southeast Sulawesi. Rhizobacteria application is one alternative method to control this pathogen. The aim of this experiment was to study of rhizobacteria indigenous formulation to control of Rhizoctonia root rot disease and increase soybean yield in Ultisol soil. A complete randomized design with seven treatments was used in this experiment. The treatments were (A) rhizobacteria formulation for seed treatment, (B) rhizobacteria formulation for seed treatment and repeated at 2 weeks after planting, (C) rhizobacteria formulation for seed treatment, repeated at 2 and 4 weeks after planting, (D) rhizobacteria formulation for seed treatment and fungicide applied at 2 weeks after planting, (E) fungicide seed treatment, and repeated at 2 weeks after planting, (F) fungicide seed treatment, and repeated at 2 and 4 weeks after planting, and control (without rhizobacteria and fungicides). All treatments were inoculated by R. solani and replicated three times. The results showed that rhizobacteria seed treatment and repeated at 2 and 4 weeks after planting was the most effective treatment to control Rhizoctonia root rot disease, and increase plant height and leaf number up to 119% and 170%, respectively, and increased the yield of soybean up to 1870% in ultisol soil compared to plant with control treatment.

Karakteristik Morfologi dan Fisiologi Isolat Pantoea stewartii subsp. stewartii pada Jagung Yulfi Desi; Trimurti Habazar; Agustian Agustian; Ujang Khairul; Syamsuwirman Syamsuwirman; Prima Novia
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Pantoea stewartii subsp. stewartii is the pathogen causing stewart’s wilt disease in maize. The occurrence of the disease was recently reported in Indonesia with limited information. Research was conducted to characterize several isolates of P. stewartii subsp. stewartii isolated from maize plants collected from 6 districts in West Sumatra. Leaves showing yellow stripes symptoms along the vein was ground and suspeneded then the bacteria was isolated and incubated for 3 × 24 h at room temperature in nutrient glucose agar (NGA). Pure bacterial colonies gained from this medium was used for characterization. Assessment of bacterial characters was conducted based on morphological assay, physiological assay, biological assay (hypersensitivity, and pathogenicity) followed by polymerase chain reaction (PCR)-based identification. Unique characters of the family Enterobacteriaceae was observed on 7 isolates based on morphological and physiological characters, and only 4 isolates (PR1, PP, ST1, LA) gave positive reaction on hypersensitivity and pathogenicity test. Further molecular identification confirmed 3 out of 7 isolates had high homology to Pantoea stewartii, i.e. Pantoea stewartii strain R1-104, R1-132, and ATCC 29923 for isolates LA, PR1, and PR2, respectively.

Analisis Keragaman Genetik Isolat Bakteri Xanthomonas oryzae pv. oryzae dari Jawa Barat dan Jawa Tengah Berdasarkan Analisis ARDRA Gen 16SrRNA Yadi Suryadi; Dwi N. Susilowati; Puji Lestari; Sutoro Sutoro; Ifa Manzila; Trini Suryani Kadir; Sheryn Sunni Albani; I Made Artika
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Fifteen Xanthomonas oryzae pv. oryzae isolates from several regions in West Java and Central Java have been studied for their genetic diversity using amplified ribosomal DNA restriction analysis (ARDRA) 16SrRNA gene.Total of 13 ARDRA patterns from digestion of DNA with RsaI restriction enzyme was obtained, indicated higher genetic diversity of X. oryzae pv. oryzae. Phylogenetic analysis revealed a close genetic relationships among isolates. Isolates X. oryzae pv. oryzae 1/96pml and 61pml are closely related with isolates 29d, 59pml, and 60pml although they are isolated from different locations. The other closely related isolates were between X. oryzae pv. oryzae 5mgl with 23d, 38d, 10sbg, 8myd; while 6klt was close to 3ind and 2kr. The result of X. oryzae pv. oryzae genetic diversity can be used as a reference for strain or/isolates selection for development ofbacterial leaf blight resistance in Indonesia.

Keefektifan Bakteri Endofit dan Bakteri Perakaran Pemacu Pertumbuhan Tanaman dalam Menekan Penyakit Layu Bakteri pada Tomat Zhenita Vinda Tri Handini; Abdjad Asih Nawangsih
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial wilt disease caused by Ralstonia solanacearum is an important disease on tomato. Endophytic bacteria and plant growth promoting rhizobacteria has been reported as potential biocontrol agent for many plant diseases. Research was conducted to determine the effectiveness of some isolates of endophytic bacteria (BC10, Bacillus amyloliquefaciens, dan Staphylococcus epidermidis BC4) and plant growth promoting rhizobacteria (Pseudomonas fluorescence RH4003, Bacillus subtilis AB89) as biocontrol agents for tomato wilt disease. Bacterial isolates was applied as soil drench on tomato seedling in single or combination treatments. Combination of P. fluorescens RH4003 and isolate BC10 (F1*BC10) enhanced the highest plant height, although plant dry mass among treatments was not significantly different. Single application of S. epidermidis BC4 was significantly suppressed disease incidence.

Efektivitas Isolat Bakteri dari Rizosfer dan Bahan Organik Terhadap Ralstonia solanacearum dan Fusarium oxysporum pada Tanaman Kentang Tutik Kuswinanti; Baharuddin Baharuddin; Sri Sukmawati
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Rhizosphere is an ideal area for growth and development of microbial antagonists, because it is a nutrition rich area. Nutrients that secreted in the rhizosphere are influenced by many environmental factors which in turn effect the diversity and abundance of microbial community. Based on these facts, the research were aimed to isolate bacteria from the rhizosphere and organic materials in Tana Toraja, and determine their role as biological control agents for potato wilt disease. Antagonistic test was conducted in order to determine the ability of each bacterial isolate to inhibit the growth of Ralstonia solanacearum and Fusarium oxysporum. The results showed that from totally 74 bacterial isolates, only 10 and 4 bacterial isolates were able to inhibit the growth of R. solanacearum and F. oxysporum in vitro, respectively.

Page 7 of 42 | Total Record : 412

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