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Jurnal Akuakultur Indonesia
ISSN : 14125269     EISSN : 23546700     DOI : -
Core Subject : Agriculture,
Jurnal Akuakultur Indonesia (JAI) merupakan salah satu sarana penyebarluasan informasi hasil-hasil penelitian serta kemajuan iptek dalam bidang akuakultur yang dikelola oleh Departemen Budidaya Perairan, FPIK–IPB. Sejak tahun 2005 penerbitan jurnal dilakukan 2 kali per tahun setiap bulan Januari dan Juli. Jumlah naskah yang diterbitkan per tahun relatif konsisten yaitu 23–30 naskah per tahun atau minimal 200 halaman.
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Articles 569 Documents
The Effect Hydroquinone Extracted from Sonneratia caseolaris Fruit to Control Vibrio harveyi Artificial Infection on Tiger Shrimp, Penaeus monodon Fab. Arifuddin, .; Sukenda, .; Dana, D.
Jurnal Akuakultur Indonesia Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.321 KB) | DOI: 10.19027/jai.3.29-35

Abstract

The role of hydroquinone extracted from Soneratia caseolaris fruit to control Vibrio harveyi infection on tiger prawn was carried out. In vitro experiment was conducted using disc diffusion and MIC {minimum inhibitory concentration) methods to know the sensitivity of V. harveyi to hydroquinone. Two kinds of in vivo experiments were (1) hydroquinone was injected into shrimps muscle and a week later the shrimps were challenged with V. harveyi (2) the shrimps were challenged with V. harveyi and one day later hidroquinone was injected. Total count of live V. harveyi on the shrimps and survival rate were observed after challenge test. Hydroquinone showed antibacterial activity with MIC at 3000 ppm. Hydroquinone injected shrimp showed higher survival rate compared with control (100% vs 50%). Total count of V. harveyi from injected shrimp, either before or after challenged, decreased by 2,61xl04 cfu/g and l,61xl04 cfu/g, respectively. These findings indicated that crude hydroquinone have anti-bacterial effect to control V. harveyi infection. Key words: hydroquinone, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon   ABSTRAK Telaah peran hidrokuinon yang diekstraksi dari buah Soneratia caseolaris untuk mengontrol infeksi Vibrio harveyi pada udang windu dilakukan. Percobaan in vitro dilakukan menggunakan metode difusi cakram dan MIC {minimum inhibitory concentration) untuk mengetahui sensitivitas V. harveyi terhadap hidrokuinon. Percobaan in vivo dilakukan dengan dua cara (1) hidrokuinon disuntikkan pada otot udang dan seminggu kemudian udang diuji tantang dengan V. harveyi (2) udang ditantang terlebih dahulu dengan V. harveyi dan sehari kemudian hidrokuinon disuntikkan. Jumlah total bakteri V. harveyi hidup pada udang dan kelangsungan hidup udang diamati setelah uji tantang. Hidrokuinon menunjukkan aktivitas antibakteri dengan MIC 3000 ppm. Udang yang diinjeksi dengan hidrokuinon mempunyai kelangsungan hidup yang lebih tinggi dibandingkan kontrol (100% vs 50%). Jumlah total V. harveyi pada udang yang diinfeksi, baik sebelum maupun sesudah, masing-masing turun sampai 2,61 x 104 cfu/g dan 1,61 x 104 cfu/g. Hasil ini menunjukkan bahwa hidrokuinon mempunyai efek anti-bakterial untuk mengontrol infeksi V. harveyi. Kata kunci: hidrokuinon, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon
Resistance of Fry from Vaccinated Mother of Gift Tilapia (Oreochromis niloticus Linn.) to Artificial Infection of Streptococcus iniae Nur, I.; Sukenda, .; Dana, D.
Jurnal Akuakultur Indonesia Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.466 KB) | DOI: 10.19027/jai.3.37-43

Abstract

An investigation was made to study the efficacy of dosage and time of administration in maternal vaccination. Mothers of nile tilapia were vaccinated by intraperitoneal injection with adjuvant-heat killed Streptococcus iniae at one and two weeks after spawning at the dosages of 0,2 ml/kg and 0,4 ml/kg body weight. Unvaccinated mothers were used as control. Agglutinating antibody titers in the blood plasma of mothers before and after spawning, eggs soluble extract, the body fluid of fry at 5, 10 and 15 days post hatching (DPH) were examined. The protective immunity of fry was tested by challenge test, the survival rate (SR) and the relative percent survival (RPS) offish within 7 days was observed. There was no difference in the antibody level of mothers, eggs soluble extract, and the body fluid of fry at 5, 10 and 15 DPH from vaccinated mothers at one or two weeks after spawning. However, antibody of mothers, eggs and fry from vaccinated mothers at the dosage of 0,4 ml/kg body weight were higher than the dosage of 0,2 ml/kg body weight and control. Antibody titre. of fry of vaccinated mothers at 5, 10 and 15 DPH were (-log2=2,88), (-log2=2,53) and (-log2=2,07) respectively, while SR were 89%, 94% and 92% respectively. SR of control fry were lower 7%, 10% and 12% respectively than fry from vaccinated mother; meanwhile RPS were 47%. 76% and 77% respectively. Key words: Maternal immunity. Streptococcus iniae, Oreochromis niloticus, vaccination, antibody   ABSTRAK Suatu studi tentang efikasi dari dosis dan waktu pemberian vaksin pada vaksinasi lewat induk dilakukan. Induk ikan nila (Oreochromis niloticus) diberi vaksin melalui injeksi intraperitoneal dengan "adjuvant-heat killed Streptococcus iniae" satu minggu sesudah memijah dan dua minggu sesudah memijah dengan dosis 0,2 ml/kg dan 0,4 ml/kg bobot tubuh. Aglutinasi titer antibodi dari plasma darah induk sebelum dan sesudah memijah, ekstrak terlarut telur, dan cairan tubuh pada 5, 10 dan 15 hari setelah menetas (DPH) diperiksa. Imunitas dari larva diuji dengan uji tantang, kelangsungan hidup (SR) dalam 7 hari setelah uji tantang diamati. Hasil menunjukkan bahwa tidak ada perbedaan pada level antibodi dari induk, ekstrak terlarut telur dan cairan tubuh dari larva pada 5, 10 dan 15 DPH dari induk yang diberi vaksin pada satu atau dua minggu setelah memijah. Akan tetapi, level antibodi induk, ekstrak terlarut telur dan cairan tubuh larva dari induk-induk yang diberi vaksin dengan dosis 0.4 ml/kg bobot tubuh lebih tinggi dari 0.2 ml/kg bobot tubuh dan kontrol. Level antibodi dari larva yang berasal dari induk yang diberi vaksin dengan dosis 0.2 ml/kg, 0.4 ml/kg dan kontrol adalah (-log2=2.5), (-log2=2.9) dan (-log2=2). Sedangkan kelangsungan hidupnya masing-masing 95%, 94% dan 84%. Kata kunci: Imunitas induk. Streptococcus iniae, Oreochromis niloticus, vaksinasi, antibodi
Correlation between Number of Spot with Body Length in Humback Grouper, Cromileptes altivelis Y. Yahyadi; R.S. Aliah; M. Murdjani; K. Sumantadinata
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.008 KB) | DOI: 10.19027/jai.3.1-4

Abstract

The study with the objective to identify correlation between the number of black spots (dot) and total length was conducted in the humpback grouper. The spots were counted from 423 samples offish which ranged from 1.2 cm to 53.0 cm in size. The numbers of spots were observed at the body, head and fins. The simple linier regression was used to analyze correlation between the number of spots and total length. The regression between total spots (Yi) and total length (X) is Yi = 16.386 X + 80.754; the total spots of the body (Y2) and total length is Y2= 4.230 X + 1.548; the spots of the head (Y3) and total length is Y3 = 3.907 X + 31.728, and the total spots of the fins (Y4) and total length is Y4 = 5.066 X + 42.802. In general, there was a positive correlation between number of black spot and total length. A duplication like mitotic division. The process of black spots duplication probably similar to mitotic division of cell. Key words : Grouper, Cromileptes altivelis, spot (dot), body lenth   ABSTRAK Penelitian bertujuan untuk mengidentifikasi korelasi antara jumlah totol hitam pada ikan kerapu bebek dengan pertambahan panjang tubuhnya. Pengamatan totol dilakukan terhadap 423 ekor kerapu bebek yang berukuran panjang total 1,2 cm sampai 53,0 cm yang diperoleh dari Balai Budidaya Air Payau (BBAP) Situbondo, Jawa Timur. Jumlah totol dihitung pada bagian badan, kepala dan sirip. Data dianaiisis dengan menggunakan regresi linier sederhana. Persamaan regresi linier keseluruhan jumlah totol (Y|) dengan panjang total (X) adalah Y, = 16.386 X + 80.754, jumlah totol pada badan dengan panjang badan Y2 = 4.230 X + 1.548, jumlah totol pada kepala (Y3) dengan panjang badan adalah Y3 = 3.907 X + 31.728, dan jumlah totol pada sirip (Y4) dengan panjang badan adalah Y4 = 5.066 X + 42.802. Secara umum ada korelasi positif antara jumlah totol dengan panjang total. Proses pertambahan jumlah totol diduga seperti pada proses perbanyakan sel secara mitosis. Kata kunci : Ikan kerapu, Cromileptes altivelis, totol, panjang tubuh
Morfological Variation of Endemic Fish Rainbow Celebensis (Telmatherina celebensis Boulenger) in Lake Towuti, South Sulawesi Nasution, S.H.; Sulistiono, .; Sjafei, D.S.; Haryani, G.S.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.492 KB) | DOI: 10.19027/jai.3.5-11

Abstract

Rainbow Celebensis (Telmatherina celebensis Boulenger) is one of endemic fish is a part of richness of biodiversity and world heritage in Lake Towuti. Rainbow Celebensis have beautiful colour especially in male, so that it is an economically and potentially as ornamental freshwater fish. It should be protected from threats because to fear decrease offish population in nature. This research is to know morphological variation of endemic fish rainbow Celebensis based on standard morphometric character in several stations. This research is conducted in Lake Towuti, South Sulawesi from March 2002 until April 2003 in four stations were I: Bakara Cape, II: inlet of Lake Towuti for River Tominanga, III: Loeha Island, IV: outlet of Lake Towuti to River Hola-hola. Sample were collected using experimental gillnet sized of 3A, 1, lA, and VA inch. Measuring offish standard morphometric character to be down on Kottelat et al. (1993) with modification covering 14 characters. The result of univariate and multivariate analysis towards standard morphometric character, could be said that male and female at I, II, III, and IV station is same tendency or to be deacended from one fish population. The characteristic to have influence of male are body hight and length from mouth to first pectoral fin, whereas in female are forskal length, total length, and basic length of second pectoral fin. Key words: Morphological variation. Telmatherina celebensis, lake Towuti.   ABSTRAK Rainbow Selebensis adalah salah satu jenis ikan endemik dan merupakan bagian dari kekayaan sumberdaya hayati dan world heritage, yang terdapat di Danau Towuti. Rainbow Selebensis memiliki warna tubuh yang indah, terutama pada ikan jantan sehingga ikan tersebut berpotensi sebagai ikan hias air tawar yang bernilai ekonomis. Ikan ini perlu dilindungi dari ancaman kepunahan karena dikhawatirkan akan terjadi penurunan populasi ikan tersebut di alam. Penelitian ini bertujuan untuk melihat variasi morfologi ikan rainbow Selebensis berdasarkan karakter morfometrik baku pada beberapa stasiun. Penelitian dilakukan di perairan Danau Towuti, Sulawesi Selatan dari bulan Maret 2002 hingga April 2003 pada empat stasiun, yaitu I: Tanjung Bakara, II: inlet Danau Towuti yang berasal dari Sungai Tominanga, III: Pulau Loeha, dan IV: outlet Danau Towuti yang mengalir ke Sungai Hola-hola. Sampel ikan diperoleh dengan menggunakan jaring insang eksperimen (experimental gillnet) terdiri dari empat ukuran mata jaring yaitu 3A , 1, VA . dan VA inci. Pengukuran karakter morfometrik ikan menggunakan metode baku yang mengacu pada Kottelat et al. (1993) yang dimodifikasi yang meliputi 14 karakter. Berdasarkan hasil analisis univariat dan multivariat terhadap karakter morfometrik baku di setiap stasiun, dapat dikatakan bahwa ikan jantan dan betina pada stasiun I, II, III, dan IV cenderung sama atau berasal dari satu kelompok populasi ikan. Karakter yang paling berpengaruh pada ikan jantan dicirikan oleh tinggi badan dan panjang dari mulut ke sirip punggung pertama, sedangkan pada ikan betina dicirikan oleh karakter panjang forskal, panjang total, dan panjang dasar sirip punggung kedua. Kata kunci: Variasi morfologis. Telmatherina celebensis, Danau Towuti
The Caryotipe of Endemic Ornamental Fish Celebes Rainbow (Telmatherina ladigesi) from Maros River, South Sulawesi Andriani, I.; Sugiri, N.; Carman, Odang; Sjafei, D.S.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.607 KB) | DOI: 10.19027/jai.3.13-18

Abstract

The objective of this research was to find the caryotype of the endemic ornamental fish, Celebes rainbow (Telmatherina ladigesi) from Maros River South Sulawesi, Indonesia. The experimental fish were caught from the branches of Maros River, those are Bantimurung River, Pattunuangasue River and Makkatoang River. Tissue collection and slide preparation were carried out using the solid tissue technique with slight modification. The amount of chromosome pairs found from those three rivers (three population) were 2n = 48. These chromosomes were divided into 13 pairs big chromosomes (>1 urn) and 11 pairs small chromosomes (I u,m) dan 11 pasang kromosom kecil (
Control of Natural Food with Diazinon for Growth and Survival of Marbled Goby, Oxyeleotris marmorata (Bleeker) Nasir, M.; Sumawidjaja, K.; Effendi, Irzal
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.623 KB) | DOI: 10.19027/jai.3.19-24

Abstract

Dua percobaan telah dilakukan, yaitu tanpa dan dengan larva ikan. Percobaan pertama, yang menggunakan 3 konsentrasi diazinon 0. 2 dan 4 ppm dan 3 ulangan, mempelajari pertumbuhan rotifera, cladocera dan copepoda. Percobaan kedua mempunyai 2 perlakuan, yaitu diazinon 0 dan 4 ppm (yang terbaik dari percobaan pertama) serta 4 ulangan untuk mengevaluasi: 1) ketersediaan, pemanfaatan dan susunan jasad-jasad pakan, 2) pertumbuhan dan kelangsungan hidup larva, serta 3) perkembangan larva ikan. Larva yang berumur satu hari ditebar pada saat kelimpahan rotifera tertinggi di hapa dengan kepadatan 40 larva/1 atau 3.200 larva/hapa. Hapa (mesh 0,8 mm) yang berukuran 50x40x50 cm ditempatkan dalam kolam-kolam beton yang berukuran 4,25x2,00x 0,65 m. Kolam-kolam ini mula-mula dikeringkan selama 2 hari, dipupuk dengan kotoran ayam 1.000 g/m3 dan diisi air setinggi 50 cm. Keesokan harinya kolam dipupuk dengan urea dan tripel superfosfat masing-masing 20 dan 30 g/m3. Dua hari kemudian air diberi diazinon sesuai perlakuan. Hasil percobaan pertama menunjukkan bahwa diazinon meningkatkan ketersediaan rotifera. Populasi rotifera tertinggi dicapai di kolam yang mendapat diazinon 4 ppm. Percobaan kedua memberikan laju pertumbuhan harian dan kelangsungan hidup larva ikan serta ketersediaan dan pemanfaatan rotifer tertinggi pada pemberian diazinon 4 ppm. Kata kunci: Pakan alami, larva. Oxyeleotris marmorata, diazinon. kelangsungan hidup, pertumbuhan   ABSTRACT Two experiments were conducted, without and with fish larvae. The first experiment, utilizing 3 concentrations of diazinon, i.e. 0, 2 and 4 ppm and 3 replications, evaluated the growth of rotifers, cladocerans and copepods. The second experiment had 2 treatments, i.e. 0 and 4 ppm diazinon and 4 replications evaluated: 1) the availability, utilization and composition of food organisms, 2) the growth and survival rates offish larvae, and 3) the development of larvae. One-day old larvae were stocked at times of highest rotifer concentrations in hapas at 40 larvae/1 or 3,200 larvae/hapa. Hapas (0,8 mm mesh) of 50x40x50 cm were placed in concrete ponds of 4.25x2.00x0,65 m. The ponds were dried for 2 days, fertilized with chicken manure 1.000 g/m3 and filled with water up to 50 cm deep. Next day, the ponds were fertilized with urea and triple superphosphate 20 and 30 g/m3 respectively. Two days later, the water was treated with diazinon according to treatments. The results of the first experiment showed that diazinon increased the availability of rotifers. The highest rotifer populations were obtained in ponds receiving 4 ppm diazinon. The second experiment gave highest daily growth and survival rates of fish larvae, te availability and utilization of rotifers at 4 ppm diazinon. Key words: Natural foods, larvae, Oxyeleotris marmorata, diazinon, survival, growth.
Pengaruh Naupli Artemia yang Diperkaya dengan N-3 HUFA terhadap "Budget" Energi Larva Ikan Red Sea Bream Pagrus major Sumule, O.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.422 KB) | DOI: 10.19027/jai.3.25-35

Abstract

Energy budget of red sea bream Pagrus major larvae fed n-3 highly unsaturated fatty acids (HUFA)-enriched (EA) and non-enriched (NEA) Artemia nauplii was constructed as: EI = F + M + U + G, where EI is energy intake, F energy loss as feces, M energy loss for metabolism, U energy loss as non-fecal matter based on ammonia excretion, and G energy for growth. Larvae (29 days post hatching, 41,1 mg mean wet weight) were reared in six 80 1 circular tanks and fed EA and NEA for 12 days, with three replicates for each type of food. Overall, growth of larvae was significantly higher in EA group than NEA group. Oxygen consumption, as heat increment, was also significantly higher in EA-fed larvae than NEA-fed larvae. The energy budget of a 100-mg larva was partitioned into: 100% of EI = 38,4% for G + 34,5% for M + 2,9% for U + 24,2% for F, for EA group, whereas 100% of EI = 29,1% for G + 30,1% for M + 3,3% for U + 37.4% for F, for NEA group. Assimilation, gross conversion, and net conversion efficiencies were higher in EA-fed larvae than NEA-fed larvae, which were attributed to the higher energies channeled to metabolism and growth in the former. This study concluded that HUFA enrichment of Artemia nauplii increased energy absorption but reduced energy excretion in red sea bream larvae. Key words : Pagrus major, larvae. HUFA enriched Artemia nauplii, oxygen consumption, ammonia excretion, heat increment, energy budget
The Improvement of Protein Retention through a Higher Utilization of Carbohydrate in the Diet with Chromium for Common Carp Cyprinus carpio Linn. Mokoginta, I.; Hapsyari, F.; Suprayudi, M. Agus
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.7 KB) | DOI: 10.19027/jai.3.37-41

Abstract

This experiment was conducted to improve the protein retention through carbohydrate efficiency in the chromium diet of common carp Cyprinus carpio. Four experimental diets with isonitrogen and isocaloric but different Cr+3 level 0,00; 1,33; 2,23 and 3,96 ppm were used in this experiment. Fish with body weight of 5,31 ± 0,02 gram were reared in aquaria (50 x 40 x 35 cm); 20 fish per aquarium. Fish fed upon the experimental diets three times daily, at satiation for 50 days. Results of this experiment showed that the protein retention could be improved by given Cr+3 in the diet. Based on the respon curve of protein retention (Yi = -1.1678X2 + 3,7223X + 24,818 ; R2 = 0,9) it was found that the optimum Cr*J in the diet was 1,59 ppm. The maximum relative growth rate was found at 2,16 ppm Cr3+ (Y2 = -14,592 X: + 63,18IX + 426,43 ; R = 0,9). The feed efficiency and the survival rate of the fish are the same between treatment (p>0.05). The protein level of the fish from treatment 3,96 ppm Cr+3 was lower than the others; the lipid level of Cr+3 diets was higher than that of non Cr+3 diet; however the carbohydrate level of fish from diet 2,23 Cr+3 was lower than the others. It was concluded that the optimum level of Cr +3 in the diet of common carp was 1,59-2,16 ppm Key words : dietary chromium, common carp, Cyprinus carpio   ABSTRAK Penelitian ini bertujuan untuk meningkatkan retensi protein dan pertumbuhan ikan mas Cyprinus carpio melalui peningkatan efisiensi karbohidrat pakan yang diberi kromium (Cr"3). Empat macam pakan dengan kadar Cr+3 yang berbeda yaitu 0,00; 1,33; 2,23 dan 3,96 ppm tetapi kadar protein dan energi yang sama digunakan pada penelitian ini. Ikan mas berbobot 5,31 ± 0,02 gram per ekor dipelihara dalam akuarium (50x40x35 cm) dengan kepadatan 20 ekor. Ikan diberi pakan tiga kali sehari secara at satiation. Pemberian pakan dilakukan selama 50 hari. Hasil penelitian memperlihatkan bahwa adanya pemberian Cr+3 dalam pakan akan mengefisienkan penggunaan karbohidrat pakan sehingga retensi protein akan meningkat pula. Retensi protein tertinggi dicapai pada kadar Cr+3 1,59 ppm (dihitung dari persamaan y, = -1,1678X2 + 3,7223X + 24,818; R2 = 0,9). Pertumbuhan relatif tertinggi dicapai pada kadar Cr+3 2,16 ppm (dihitung dari y2 = -14,592 X2 + 63.181X + 426,43; R2 = 0,9); sedangkan efiseinsi pakan dan tingkat kelangsungan hidup antar perlakuan sama (p>0,05). Kadar protein tubuh perlakuan 3,96 ppm Cr+3 relatif lebih rendah dari perlakuan lainnya. Kadar lemak tubuh ikan yang diberi Cr+3 lebih tinggi dari yang tidak diberi Cr+3; sedangkan kadar karbohidrat tubuh terendah pada perlakuan 2,23 ppm Cr+3. Secara keseluruhan dapat disimpulkan bahwa kadar Cr+3 optimum dalam pakan ikan adalah 1,59-2,16 ppm. Kata kunci : Kromium pakan. ikan mas, Cyprinus carpio
The Improvement of Protein Retention through a Higher Utilization of Carbohydrate in the Diet with Chromium for Common Carp Cyprinus carpio Linn. S, Nuryati; Dana, D.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.452 KB) | DOI: 10.19027/jai.3.43-46

Abstract

Primary shrimp cell culture from lymphoid organ of Penaeus monodon was successfully developed. Minced tissues of lymphoid organ were seeded by tripsin enzyme and culture in Leibovit'z-15 supplemented with 10% fetal bovine serum and 5% NaCl. Plates were then incubated at 27 °C with 5% C02 supply. Confluent cells were infected by white spot baculovirus (WSBV). Cytopathic effect (CPE) induced by white spot baculovirus showed characteristics of detachment cells and lysis forming giant polycaryon cells. Primary shrimp cell culture could be continued by anvanced cell culture resulting cell line could be used virus media. Key words : cell culture, lymphoid organ, tiger shrimp, invitro, virus   ABSTRAK Kultur sel primer dari organ limfoid udang windu Penaaeus monodon telah berhasil dilakukan. Potongan organ limfoid dipisahkan menjadi sel-sel tunggal dengan menggunakan enzim tripsin. Sel-sel tunggal tersebut dikuitur dalam media Leibovit'z-15 dengan tambahan 10 % fetal bovine serum dan 5 % NaCl. Biakan diinkubasi pada suhu 27 °C dengan kandungan C02 sebanyak 5 %. Biakan yang telah konfluen diinfeksi dengan virus WSBV dan menghasilkan cytopathic effect (CPE) dengan karakter sel lepas dari perlekatan dan mengalami lisis yang menyisakan giant cells dengan inti banyak (polycaryons). Kultur sel primer ini dapat ditindaklanjuti dengan kultur sel dengan pasase yang berulang-ulang sehingga dapat menghasilkan sel lestari (cell line) untuk media tumbuh virus. Kata kunci: biakan sel, organ limfoid, udang windu, invitro, virus
Control of Polychaetes by Dipping Infected Pearl Oyster on Different Salinity Hadiroseyani, Yani; Iswadi, .; Djokosetyanto, Daniel
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.703 KB) | DOI: 10.19027/jai.3.47-49

Abstract

Dip treatment on pearl oysters (Pinctada maxima) was conducted in different concentrations of saline water to eliminate boring polychaetes. Results shows that polychaetes leave the osyters which treated on saline water at 0 ppt, 45 ppt, and 60 ppt as long as 15 minutes each. It also shows that the oysters got high survival rate 7 days after the treatment. Key words : Polychaetes, pearl oyster, dipping, salinity   ABSTRAK Pengendalian polikaeta pengebor dengan menggunakan berbagai konsentrasi larutan garam telah dilakukan pada tiram mutiara (Pinctada maxima). Berdasarkan jumlah polikaeta yang keluar, hasil percobaan ini menunjukkan bahwa perendaman dengan konsentrasi garam 0 ppt. 45 ppt, dan 60 ppt selama 15 menit lebih efektif dibandingkan pada konsentrasi 15 dan 30 ppt. Tingkat kelangsungan hidup tiram mutiara yang diamati 7 hari setelah perendaman dalam larutan garam mencapai 100%. Kata kunci : Polikaeta. tiram mutiara, perendaman. salinitas

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