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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 98 Documents
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Search results for , issue "Vol. 11 No. 2 (2023): December" : 98 Documents clear
Etnobotani Tumbuhan Obat yang Dijual di Blok Bahan Jamu Pasar Raya I Salatiga Ananda, Raditya Se'o; Suprihati, Suprihati
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9486

Abstract

Ethnobotany is a branch of science that studies the correlation between humans and plants. Generally, the basic ingredients for making herbal medicine come from types of medicinal plants. The majority of city residents buy medicinal plants from markets or drug stores. Pasar Raya I Salatiga as a source of meeting the needs of the people of Salatiga City. The aim of this research is to know the types of medicinal plants being sold, knowing the parts of the medicinal plants being sold, knowing the shape of the medicinal plants being sold, knowing how to obtain the medicinal plants being sold, and knowing the agrotechnological engineering actions of one of the medicinal plants being sold. This research is a qualitative descriptive research. This research was conducted with three herbal medicine sellers, three consumers, and three ginger farmers. This research was conducted in August-September 2023. Data collection techniques used semi-structured interviews, observation, literature study and documentation. Data processing with simple statistics. The research results found a total of 92 medicinal plants. The parts of medicinal plants sold include roots (4%), stems (7%), seeds (19%), fruit (16%), flowers (4%), leaves (26%), leaves & flowers (1%), herbs (1%), bark (4%), rhizomes (15%), and tubers (3%). The forms of medicinal plants sold include dry (71%), fresh (17%), dry & fresh (7%), and processed (5%). All medicinal plants are obtained by purchasing. Agrotechnological engineering actions carried out by ginger farmers include applying fertilizer, planting in the right season and scheduling planting.
Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis Widyaningrum, Trianik; Rizqiyah, A’isyah Arroobi’atu
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9487

Abstract

The need for fuel oil in Indonesia is increasing along with the increase in the number of people's consumption in the fields of transportation, industry, and economy. While the source of petroleum fuel in Indonesia is running low. Therefore, it is necessary to utilize alternative energy, namely bioethanol. Bioethanol can be produced from organic waste that contains high cellulose, one of which is peanut shells. This study aims to determine the effect of the ratio of crude cellulase enzyme Trichoderma reesei and Aspergillus niger on sugar and ethanol content of peanut shell fermentation using Zymomonas mobilis, and to determine the ratio of crude cellulase enzyme that produces the highest sugar and ethanol content of peanut shell fermentation using Z. mobilis. This research is an experimental study with independent variables, namely the ratio of crude cellulase enzyme T. reesei : A. niger (0:0), (1:0), (0:1), (1:1), (1:2), (2:1), (1:3), and (3:1) and the dependent variable of sugar and biethanol content. Data analysis of experimental research results using ANOVA and DMRT test. The addition of crude cellulase enzyme from T. reesei and A. niger affects the sugar content and bioethanol from peanut shell fermentation. The ratio of crude cellulase enzyme from T. reesei and A. niger that produces the highest sugar content is in the treatment P5 ratio of crude enzyme T. reesei: A. niger (2:1) which is 0.14 g/mL and the highest ethanol content from groundnut shell fermentation is in the treatment of P8 crude enzyme ratio of T. reesei : A. niger (1:3) which is 2.23%.
Pengaruh Konsentrasi Crude Enzim Bacillus subtilis terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Singkong Menggunakan Zymomonas mobilis Nurusman, Arief Abdillah; Widyaningrum, Trianik; Nisa, Rifda Khairun; Utami, Listiatie Budi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9491

Abstract

Bioethanol is an environmentally friendly and renewable alternative fuel. The use of alternative fuels is necessary because petroleum fuels make a major contribution to increasing greenhouse gas concentrations, especially CO2. Bioethanol can be obtained from materials containing carbohydrates such as cassava peel which has a high carbohydrate content, namely 4.55%. The aim of this research was to see the effect of the crude enzyme concentration of Bacillus subtilis on the results of sugar and bioethanol content of cassava peel fermentation with Zymomonas mobilis and to find out the crude enzyme concentration that had the greatest influence. This research is an experimental study with the independent variable, namely the concentration of crude cellulase enzyme B. subtilis with concentrations of 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% , and the dependent variables are sugar content from hydrolysis and bioethanol content from the fermentation process. The data obtained were analyzed using the ANOVA test and Duncan's test. The results of research on the effect of crude Bacillus subtilis cellulase enzyme concentrations of 8 concentrations have an influence on the amount of sugar and bioethanol produced from fermentation of cassava peel with Zymomonas mobilis. Crude Bacillus subtilis cellulase enzyme with a concentration of 17.5% had the greatest influence on sugar and bioethanol levels with a sugar content of 0.37% and a bioethanol content of 1.67%.
Meta-Analisis : Efektivitas Multimedia Interaktif terhadap Peningkatkan Hasil Belajar Siswa Khaeruman, Khaeruman; Sudiatmika, AAIA Rai; Suma, I Ketut; Suardana, I Nyoman
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9497

Abstract

This research aims to determine the effectiveness of interactive multimedia on student learning outcomes by analyzing 15 articles that can be accessed from Google Scholar, Garuda, and Mendeley. These articles were published in non-accredited journals, accredited Sinta, and indexed by Copernicus or Scopus with 5 different sample levels, namely: Elementary School, Middle School, High School, Vocational High School, and College. The use of interactive multimedia contains text, graphics, audio and moving images (video) which can be displayed via projector and laptop screens. This research method uses a meta-analysis method, namely by comparing research data from control and experimental groups in the form of sample size (N), Standard Deviation (SD), and learning outcomes (X). The data was analyzed statistically to see the effect size value. Based on the results of the analysis test, it was found that from the 15 articles analyzed, the average effect size was 1.146 with a P-Value < 0.001 (P-Value < 0.05), which means that learning using multimedia is very effective or has an influence on student learning outcomes. Next, to see at which level the effectiveness value is higher, the analysis results found that the estimated value at the elementary school level was 1.719 (more effective), followed by the tertiary level with an estimate of 1.707.
Tren Kejadian Dengue (Incidence Rate) dan Kematian Akibat Dengue (Case Fatality Rate) di Indonesia Meyrita, Meyrita; Suwarno, Suwarno; Saidi, Nurdin; Razi, Nanda Muhammad
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9500

Abstract

Dengue Hemorrhagic Fever (DHF) is a disease that currently has significant morbidity and mortality rates. In Indonesia, the trend of DHF cases has increased and decreased every year. This study aims to analyze the development of dengue cases by considering two indicators, namely dengue incidence rate and dengue case fatality rate (CFR) during the period 2012-2022, to know the pattern of dengue disease in Indonesia, and evaluate the government's target in handling dengue cases based on IR and CFR rates. The data used is secondary data obtained from the 2021 Indonesian Health Profile and the 2022 Dengue Fever Annual Report. IR and CFR data were analyzed using Microsoft Excel which will be interpreted in the form of graphs to illustrate the trends and patterns of DHF cases in Indonesia, IR maps in 2021 and 2022 were analyzed using ArcGIS software. Based on the results of the analysis, the development of dengue cases during the period 2012-2022 experienced a misalignment between the increasing trend of IR and CFR. The IR value experienced significant fluctuations from 37.3 per 100,000 population in 2012 to 52.07 per 100,000 population in 2022, while the CFR value during the period 2012-2022 tended to decrease from 0.90% (2012) to 0.86% (2022). This pattern reflects the complex dynamics of DHF epidemiology, where an increase in cases is not always followed by an increase in fatalities. The IR and CFR achievements for 2022 have not met the government's expectations, with only 16% of districts/cities having IR values ≤ 10/100,000, while the CFR value reached 0.86%, slightly above the set target of 0.7%.
Analisis Molekuler Tilapia Lake Virus (TiLV) di Sentra Budidaya Benih Ikan Gurami (Osphronemus goramy) Jawa Timur Jumria, Andi; Amin, Muhamad; Mufasirin, Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9515

Abstract

Tilapia Lake Virus (TiLV) is a New Emerging Disease that has been defined and included in the WOAH disease list (WOAH, 2022). This virus can infect both freshwater and marine fish, causing high mortality in Ttilapia up to 90%. Reverse transcyptase method - Semi Nested Polymerase Chain Reaction (RT-snPCR) for detection of TiLV in Osphronemus goramy. TiLV was discovered for the first time in Thailand and was also found in Jogjakarta in 2021. Further research is needed to see the damage and genetic variation caused by TiLV in Osphronemus goramy. This study aims to study the genetic variability and homology of TiLV isolates in Osphronemus goramy as definitive hosts in East Java. The results showed clinical symptoms of lethargic fish, decreased appetite, abnormal behavior, reduced reflexes, sluggish movements, darker body color, congestion, thinning fins and exopthalmia. The PCR results showed that the Osphronemus goramy showing clinical symptoms of TiLV infection were all positive. The sequencing results were further identified using the BLAST program, showing the highest similarity of the genetic identity of the sample at 96.8% with TiLV from Israel (Genebank Accession Number NC 029927.1).
Meta-Analisis : Studi Pentingnya Mengukur Keterampilan Metakognitif Siswa Hajiriah, Titi Laily; Sudiatmika, AAIA Rai; Suma, I Ketut; Suardana, I Nyoman
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9567

Abstract

This study aims to find the effect size of several journals that contain articles with uniform topics. The purpose of finding effect size is to measure the extent of differences or relationships between two or more variables or groups in scientific research. Effect size provides information regarding the strength and size of the observed effect, regardless of the sample size used. By evaluating effect size, researchers can determine the practical significance of their findings and compare the results of studies conducted by other researchers. The key word of this meta-analysis study is metacognitive skills. This meta-analysis is important to get an idea of studies that report the results of measuring metacognitive skills at various levels. The research method used in this study is a meta-analysis method with an experimental approach to journal tracking using OpenMEE software with keywords used, namely metacognitive skills, metacognition, metacognition awareness. Based on meta-analysis calculations that there is a relationship between all metacognitive skill measurement articles, a p value of < 0.001 means that the analysis is significant with sig. 0.05 and I^2 above 80% indicate that heterogeneity of learning measures metacognitive skills of 93.985 and the sample effect size in this study is very high heterogeneity.
Perbandingan Pola Rasio Morfometrik dan Karakteristik Habitat Dua Spesies Ikan Cupang Alam (Betta spp.) Endemik Pulau Bangka Ramadhanu, Destra; Prananda, Mustobi; Wulandari, Ufi Ayu; Hidayat, Rahmad; Syarif, Ahmad Fahrul
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9598

Abstract

Bangka Island has extraordinary biodiversity. Bangka Island has various types of freshwater fish, including local and endemic fish. The natural betta fish Betta burdigala and Betta chloropharynx are two species of fish endemic to Bangka Island which are considered endangered. The aim of this study was to analyze the differences in morphology and habitat characteristics between these two species. This research uses a quantitative descriptive approach. The fish samples used were 10 Betta burdigala and Betta chloropharynx fish taken from Bikang Village, South Bangka Regency and Jade Bahrin Village, Bangka Regency from August to September 2023. The data obtained were analyzed descriptively and presented as graphs or tables. There are eight main characteristics based on morphometric ratios, namely Total Length (TL), Body Length (BL), Tail Length (TL), Head Length (HL), Head Height (HH), Body Width-I (BW-I), Body Width-II (BW-II), and Body Width-III (BW-III). Based on morphometric ratios, there are differences in all morphometric characters. Betta burdigala and Betta chloropharynx live in peat swamp waters, the pH of Betta burdigala water is lower than the pH of Betta chloropharynx water, the color of Betta burdigala water is more black brown, while the color of Betta chloropharynx water is clearer.
Validation of Genetic Markers for AmpC, AAC, and RNR3 Genes in Potentially Present Pathogens Organisms in Fecal Sludge Musyahadah, Ummu Syauqah Al; Hamid, Radhiyah Mardhiyah; Nasir, Alricha
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9623

Abstract

The issue of sanitation and water pollution due to fecal waste has become a serious concern in Indonesia, particularly after it was revealed that approximately 70% of the community's drinking water sources are contaminated with human feces. Determining safe waste management requirements necessitates indicators confirming the contamination-free nature of the waste management methods. Pathogen testing indicators for fecal waste are highly diverse. The current standard testing is the culture-based method for microorganisms and microscopy observation for helminths. However, molecular detection tools for these pathogens can give higher sensitivity and specificity in a relatively shorter time of technical processing. In this research, we designed primers for molecular-based diagnostics on three pathogens exhibiting extreme survival capabilities under various environmental conditions. The species-coupled genes are AmpC for Escherichia coli, AAC for Mycobacterium tuberculosis, and RNR3 for Ascaris lumbricoides. The design of molecular-based biomarkers and their specificity tests were conducted using web-based bioinformatics programs to prevent unintended dimers. Ultimately, primers were validated by conventional gradient PCR on available positive controls and a sample from processed fecal sludge. The PCR validation test showed that the designed primers were validated to amplify the intended target with the right size without unspecific bands on the positive controls. Meanwhile, the AAC gene for E. coli was detected in the sample from the fecal sludge.
Pengembangan Asessment Higher Order Thinking Skills (HOTS) Berbasis Computer Based Test (CBT) pada Mata Kuliah Prinsip Pengajaran dan Asesmen yang Efektif Tibrani, Mgs; Madang, Kodri; Anggraini, Nike
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9639

Abstract

The aim of this research is to produce a Higher Order Thinking Skills (HOTS) assessment product based on a Computer Based Test (CBT) with three types of HOTS questions (true or false, multiple choice, and fill in the blank and matching) based on the factual knowledge dimension, conceptual, procedural, and metacognitive with illustrations in the form of discourse, images, graphics, videos, animations, and interactive stimulation, as well as providing feedback after going through the logical validation, empirical validation, and respondent questionnaire stages. The CBT-based HOTS assessment product was implemented for PPG students, Faculty of Teacher Training and Education, Sriwijaya University in the Principles of Effective Teaching and Assessment course with R&D research steps. The product trial subjects in this research were limited to a small scale, namely 8 PPG Pre-Service Biology Education Study Program students, FKIP, Sriwijaya University. The technique for selecting product trial subjects in this research is the cluster sampling technique, namely a sampling method based on certain clusters. The data collection technique uses expert validation sheets and respondent questionnaires, then the data collected is analyzed quantitatively descriptively. Data obtained from questionnaires and scientific communication skills were analyzed using a rating scale. To analyze each statement or indicator, the frequency of answers for each category (answer choices) is calculated and added up. The development of HOTS questions based on the Computer Based Test in the Principles of Effective Teaching and Assessment course is based on two validation values from the validator, namely 81%, meaning that the material and language that has been designed by the researcher is valid and suitable for use. Apart from that, HOTS test questions are said to be practical if the score on the practicality criteria is at least 75%. Based on the results of the student response questionnaire, a score of 91.81% was obtained.

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