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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 123 Documents
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Search results for , issue "Vol. 12 No. 1 (2024): June" : 123 Documents clear
Analisis Parameter Kimia Kualitas Air Minum Ayam Petelur (Gallus domesticus) di Kabupaten Blitar dan Kajian Literatur Fitoremediasi Kadar Fluorida Yulianti, Eny; Wahyuningtyas, Titian Ajeng; Sholikhah, Badiatus; Yuliastuti, Rostiwi Endah; Royanudin, Moch; Nafi’ah, Sriani
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.8885

Abstract

Drinking water quality is a crucial factor that influences livestock productivity. Regular evaluation of the quality of livestock drinking water is an essential activity. Blitar Regency is known as a center for egg production from laying hens (Gallus domesticus). This study aims to evaluate the quality of livestock drinking water and provide a literature review regarding the potential for improvement through phytoremediation methods. This research method involves laboratory analysis and literature review. Laboratory analysis is carried out to determine the quality of water chemical parameters, then the results are compared with livestock drinking water standards regulated by Regulation of the Minister of Health of the Republic of Indonesia Number 32 of 2017. If a discrepancy is found with the standards, it is continued with a literature study regarding the use of phytoremediation methods as a solution. Analysis of chemical parameters, including ion levels, using a reagent kit with the help of Spectroquant Pharo 300 UV Vis spectroscopy. The results of the analysis involve iron, fluoride, manganese, nitrate, nitrite, cyanide, hardness, cadmium, zinc metal and sulfate. All parameters meet quality standards, except for Fluoride (F-) levels which exceed the threshold of 1.5 mg/L, caused by the use of groundwater sources or wells which have the potential to have higher mineral content than surface water. The potential impacts of high fluoride levels on laying hens include reduced egg production, impaired growth, reproductive disorders, decreased water consumption, and decreased body weight. The literature review highlights phytoremediation methods involving plants as an economical and effective solution. Afforestation, such as planting grass or bushes around livestock areas and wells, or through creating ponds with aquatic plants, can significantly reduce fluoride levels in livestock drinking water.
Kadar Silika (SiO2) terhadap Kelimpahan Diatom Bentik di Sungai Jamblang Kabupaten Cirebon Haque, Maharani Zahiratun; Afiati, Norma; Jati, Oktavianto Eko
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9192

Abstract

Jamblang River is a concern because it has a very turbid water color due to natural stone mining activities processed by the natural stone industry. One of the natural stones produced at the location of the Jamblang river is andesite rock which has a constituent component is silica. Silica is the second most abundant element in the Earth's crust after oxygen. Silica usually comes from minerals and rocks that dissolve in water. In fresh water, quartz silica is less soluble than amorphous silica. Enrichment of nutrients with silica favors the growth of diatoms, since diatoms need silica for cell wall biogenesis. The amount of silica present in water is not immediately absorbed by diatoms. The sampling method used is the purposive sampling method. The sampling locations set are 4 locations. The most interesting location is location 2 because there are 38 natural stone industries scattered around the location. There is also a simple linear regression statistical test on SPSS which shows the influence of silica levels on the abundance of benthic diatoms in the Jamblang river. The value of Sig. 0.000 shows that there is an influence of silica on plankton abundance in water because the value is < 0.05. The lowest silica content obtained at location 2 is also supported by the least abundance of diatoms found. The low abundance of diatoms is thought to be caused by high TSS numbers, low observed brightness, and low DO numbers.
Analisis Vegetasi Tumbuhan Tingkat Pohon di Kawasan Geopark Silokek Kabupaten Sijunjung Pertiwi, Vera; Chairul, Chairul
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9231

Abstract

Silokek Geopark Area is a geopark area that is being appointed into a national geopark consisting of 25 geodiversity, 12 biodiversity, and 17 cultural diversity sites. The objective of the study is to determine the composition and structure of vegetation of trees in the Silokek Geopark area, Sijunjung Regency. This research has been conducted from September to December 2022 by using the transect method. The plot was made of 10 plots with a size of 10x10 meters for vegetation that is placed alternately on both sides of the transect. Identification is carried out at the Andalas University Herbarium. Based on the research results, 15 families, 19 genera, 22 species, and 30 individuals were found. The co-dominant families are Moraceae (20%), Ebenaceae (16.67%), and Euphorbiaceae (13.33%). The highest important value index of Ficus Stricta was obtained at 57.86% and the lowest in the Nephelium Lappaceum at 7.36%. The plant diversity index in this area is classified as moderate (H '= 2.85). It is recommended that the government to controlling the diversity of plants in the Silokek Geopark area, considering that this area is a tourist area.
Pengaruh Larutan Hara dan Eco Enzyme terhadap Pertumbuhan Sawi Hijau (Brassica juncea L.) pada Sistem Hidroponik Jasmine, Nataya Annisa; Rachmawati, Diah
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9312

Abstract

Limited agricultural land has caused a decline in the productivity of green mustard (Brassica juncea L.) as one of the nutritious vegetables that is widely consumed by the public. Cultivating vegetables using a hydroponic wick system is a solution to increase the productivity of green mustard. The expensive price of nutrient solutions can be combined with eco enzymes as a provider of essential nutrients for plants. This research aims to determine the effect of the combination of AB mix with eco enzyme on the growth of green mustard. The research was designed with RAL, 8 treatments, and 3 replications. Treatments include A1E0=AB mix 100%+0mL/L eco enzyme, A1E1=AB mix 100%+1mL/L eco enzyme, A1E2=AB mix 100%+2mL/L eco enzyme, A1E3=AB mix 100%+3mL eco enzyme, A2E0=AB mix 75%+0mL eco enzyme, A2E1=AB mix 75%+1mL/L eco enzyme, A2E2=AB mix 75%+2mL/L eco enzyme, and A2E3=AB mix 75%+3mL/L eco enzyme. The parameters measured were plant height, number of leaves, wet weight of roots and shoots, dry weight of roots and shoots, and leaf area. Data were analyzed using analysis of variance and DMRT testing with α=0.05. The combination of AB mix with eco enzyme had no effect on plant height, number and area of leaves, and wet weight of roots and shoots. However, the highest dry weight of roots and shoots in the A2E2 treatment showed significantly different results from the control treatment (A2E0). Eco enzymes act as nutrient substitutes which are influenced by sunlight, plant metabolism and water absorption capacity.
Pertumbuhan dan Mortalitas Ikan Kuro (Eleutheronema tetradactylum) yang Didaratkan di Pelabuhan Perikanan Nusantara Kabupaten Merauke Kusmita, Ranny; Sunarni, Sunarni; Saleky, Dandi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9407

Abstract

This research aimed to determine the size structure, length-weight relationship, growth, and mortality of kuro fish (Eleutheronema tetradactylum) that landed on Nusantara Fishery Harbour, Merauke District. The samples were taken off in August-October 2022 at Nusantara Fishery Harbour, Merauke District. The metusedsing was a purposive sampling method. The measured data was the total length and weight body of kuro fish. The size structure and length-weight relationship of kuro fish were analyzed using Microsoft Excel. Meanwhile, the growth and mortality were analyzed using the FISAT II application. The result analysis shows that the range of E. tetradactylum total length landed on Nusantara Fishery Harbour is 430 – 1030 mm with a weight range of 92 – 3613 grams. The length-weight relationship of E. tetradactylum has the allometric negative growth pattern with equation W = 0,0016L2,1167 in August, W = 0,0017L2,1134 in September, and W = 0,0017L2,1077 in October. The analysis of E. tetradactylum’s growth parameter shows the value L∞ = 1053 mm, K = 0,51/year, and t₀ = -0,1201 year. The result mortality analysis of E. tetradactylum shows the total mortality (Z) was 0,78/ year, natural mortality was 0,43/year, fishing mortality (F) was 0,35/year, and exploitation rate was 0,45/year.
Pengembangan Protokol Deteksi Staphylococcus aureus Berbasis Molekuler Koentjoro, Maharani Pertiwi; Alviani, Melinda Nuril; Jatmiko, Yoga Dwi; Habibah, Laila Nur; Al Fatih, Ahmad Nuril Fuad; Kartikaningsih, Hartati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9494

Abstract

Staphylococcus aureus becomes a normal flora in humans, especially on the skin and in the nose. However, if it becomes excessive or if there are pathogenic variants, it can cause various health problems. The purpose of the study is to develop a molecular-based detection method for Staphylococcus aureus using the norA primer gene. The norA gene in Staphylococcus aureus is known to play a role in pathogenesis with its antibiotic resistance ability. This type of research is analytical observational with a cross-sectional method. The methods in the study include the isolation and identification of Staphylococcus aureus from healthcare facility equipment. Isolation and identification include bacterial isolation using Blood Agar Plate (BAP) media; isolate purification, Gram staining; biochemical tests using Mannitol Salt Agar (MSA) media, glucose tests, Voges Proskauer (VP) tests, catalase tests, and coagulase tests. Furthermore, S. aureus isolates were tested using a molecular-based method, namely Polymerase Chain Reaction (PCR). This method includes DNA isolation stages, qualitative testing with agarose gel electrophoresis, semi-quantitative testing with image J software, amplification with Real-Time Polymerase Chain Reaction (RT-PCR) using norA gene primers. The Mann-Whitney test results gave a value of p = 0.334 (p> 0.05) indicating the suitability between the culture method and the PCR method with the developed protocol in detecting Staphylococcus aureus. The developed method includes the use of base sequences in the norA gene primer, optimization of annealing and extension temperatures, and the concentration of DNA templates used.
Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Kulit Salak Wedi sebagai Kosmetik Antiaging Saputri, Romadhiyana Kisno; Al-Bari, Akhmad; Nisak, Siti Khoirun; Anti, Tika Roro; Amelia, Rika
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9590

Abstract

Wedi snakefruit is one of the local species snakefruit produced in Bojonegoro Regency. Antioxidant activity in salak fruit may be influenced by its cultivar. The peel of snakefruit is reported to have antioxidant activity as in salak fruit flesh. Antioxidants can prevent free radical damage to human skin and prevent aging so can be developed in cosmetic preparations. The current trend of antiaging cosmetic preparations leads to preparations with nanoparticle size. The objectives of this study is to determine the antioxidant activity and to characterisation of nanoparticles Wedi snakefruit peel extract that will be developed as anti-aging cosmetics. Wedi snakefruit peel extract was conducted using maceration method with 96% ethanol solvent, antioxidant activity test was conducted by DPPH method and total phenol content determination method using Folin-Ciocalteu method. Nanoparticles preparation of extract by ionic gelation method with characterisation through particle measurement using PSA and zeta potential measurement. Potential antiaging activity was measured through tyrosinase inhibition testing. The results showed the IC50 value measured by DPPH method of 56.10 ppm, total phenolic content of 13.41 mg GAE/g. PSA test results showed particle size of Wedi snakefruit peel extract 884 nm and zeta sizer value of 2 mv. Tyrosine inhibitory activity of Wedi snakefruit peel extract was 63.8%. The antioxidant activity of Wedi snakefruit peel extract is in the strong category, has not met the nanoparticle requirements and has tyrosinase inhibitory activity so that it has the potential to be developed as an antiaging cosmetic.
Analisis In Silico : Aktivitas Antiinflamasi dan Prediksi Toksisitas Senyawa Metabolit Sekunder Daun Ciplukan (Physalis peruviana L.) Fadzillah, Sania Nur; Hernawati, Diana; Putra, Rinaldi Rizal
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9592

Abstract

The way of life back to nature, including in the field of health, forms the habit of using plants as traditional medicine. Traditional medicine is indicated to be able to cure a variety of diseases. As with the leaves of Ciplukan (Physalis peruviana L.) by the people of Desa Wanasuka Kecamatan Pangalengan Kabupaten Bandung is considered to be used as a traditional medicine for inflammatory diseases such as rheumatism, asthma, and gastritis (maag) due to side effects and toxicity produced relatively small when compared with modern medicine. The aim of this study is to identify the potential of the secondary metabolite compound found in the leaves of the Ciplulan (Physalis peruviana L.) as a candidate for anti-inflammatory drugs. These research methods are molecular methods of docking through physical chemical properties testing, pharmacokinetic profiles of test compounds through site Pre-ADMET (Absorption, distribution, metabolism, excretion, and toxicity) and Lipinski Rule of Five. The results of this study showed that the binding affinity of caffeic acid compounds with PTGS2 receptors (PDB ID: 5F19) showed a more negative result compared to the comparative drugs Aspirin and Ibuprofen -7.4. There was a molecular interaction similar to Aspirin, in which the addition of amino acid residues from ASN 382 and TYR 385 produces a strong irreversible inhibition of PTGS2 (PDB ID: 5F19) and is predicted to inhibit prostaglandin H synthase and indicate that caffeic acid is capable of producing irreverible inactivation of PTGS2 (PDB ID:5F19). So potentially a candidate for a safer anti inflammatory drug for the body. Because it has almost the same or close potential as Aspirin against inflammatory diseases.
Dampak Pengolahan Air Lindi terhadap Kualitas Air Sungai dan Sumur di Sekitar TPA Regional Piyungan Yogyakarta Dari, Harnung Wulan; Suhartini, Suhartini
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9601

Abstract

Water is the main need for life, so the quality of the water used in life needs to be known. The objectives of this research are to determine the leachate management system at the IPL, determine efficiency of leachate water treatment performance at IPL, and determine impact of leachate water quality at Piyungan Regional Landfill on the river and wells water quality around the landfill. This research is quantitative descriptive research conducted at Piyungan Regional Landfill and its surroundings in April-July 2023. The data collected were results of physical, chemical, and biological parameters of the IPL Inlet and Outlet leachate water quality, Opak River after the IPL Outlet, and 3 samples of well water from the surrounding community representing distance from the landfill. The data collection methods used are observation, documentation, and analysis of water quality in the laboratory. The data obtained were analyzed using descriptive methods and compared with quality standards set by the Government. The results showed that: The leachate water management system at the IPL uses 8 water treatment tanks include: a bar screen, inlet, coagulation tank, equalization tank, sedimentation tank, SDB, ABR, aeration tank, maturation tank, chlorination tank, and Outlet. The efficiency of leachate water treatment performance at the IPL is not effective and efficient in processing leachate water. The quality of leachate water affects the water quality of the Opak River and wells around the landfill, on chemical parameters TDS, BOD, COD, DO, and microbiological parameters Total Coliform, therefore, the Regional Government need to evaluate and improve the IPL. Communities around the Landfill that use well water as their main need must be filtered and boiled.
Identifikasi Teripang Laut (Holothuroidea) dari Perairan Laut Pulau Kabaruan Berdasarkan Identifikasi Morfologi dan DNA Barcoding Rattu, Ferencia Esananda; Mege, Revolson; Manampiring, Nonny; Roring, Verawati Ida Yani; Gedoan, Sukmarayu; Mokosuli, Yermia
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9649

Abstract

Indonesia is known with its high biodiversity. One of them is sea cucumber. Various species of sea cucumbers are found in Indonesian sea in Talaud Regency at Kabaruan Island. Identification of sea cucumbers to reveal the biodiversity of sea cucumbers in Kabaruan Island, which is almost unknown. This research was to identify six samples of sea cucumber (Holothuroidea) in Kabaruan Island based on morphology and DNA barcoding using the COI gene. The differences between each species can be seen from the morphological characteristics, the length and width of sea cucumbers, papillae, pattern and color. The results showed that there were 4 species from 6 samples, which is Holothuria leucospilota, Holothuria whitmaei, Eleutherozoa sp., and Holothuria atra. That six samples with the code A1, A2, B1, C1, and C2 have a homology level of 99%-100% with the target nucleotide sequence from GenBank. Meanwhile, the sample’s code A3 has a homology level of 90% with the Eleutheroza sp. so there is a possibility that sample A3 is a new taxa that has the highest similarity to the Eleutheroza sp. From the six samples, it was found that samples A2 and B1 had the highest level of similarity which is 100%. Meanwhile, the samples with the lowest percentage values are samples A3 and B1 and samples A2 and A3, with a percentage value of 82.75%. There were 4 species from six samples found in Kabaruan Island. There is a possibility that Sample A3 is a new species that is similar to Eleutheroza sp.

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