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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,439 Documents
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Sifat Fisikokimia dan Organoleptik Bolu Gulung Gluten Free Berbahan Tepung Ketan Hitam (Oryza sativa L. var. glutinosa) dan Tepung Maizena (Amylum maydis) Michelle; Hartati, Fadjar Kurnia; Rahmiati, Retnani
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19338

Abstract

This study aims to examine the effect of black glutinous rice flour and cornstarch on the physicochemical and organoleptic properties of gluten-free sponge rolls, and find the best combination of the two ingredients. This study was conducted using a Complete Random Design (RAL) which has one factor and consists of four treatments. The treatment consisted of varying the proportion of black glutinous rice flour (H) and cornstarch (M) with the same total amount of flour (60 g) in each treatment, namely H1:M1 (60g:0g), H2:M2 (54g:6g), H3:M3 (48g:12g), and H4:M4 (42g:18g) which were repeated three times each. The results of the study show that for the manufacture of gluten-free sponge rolls, the H4M4 treatment is recommended as the best formulation because it provides the most optimal results compared to other treatments. Statistically, the H4M4 treatment showed a marked difference in some of the parameters tested (p<0.05). The formulation in the H4M4 treatment contains a crude fiber content of 2%, a moisture content of 50.91%, a development capacity of 66.19%, and a stability of 100%. The results of the likability level test showed a taste value of 4.30 (likes), aroma 4.17 (likes), color 4.25 (likes), and softness of 4.45 (likes), and produced the highest effectiveness value of 0.82.
Analisis Mutu Fisikokimia dan Organoleptik Panna Cotta Cincau Hijau (Cyclea barbata Miers) Liu, Louissa Calista; Hartati, Fadjar Kurnia; Rahmiati, Retnani
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19341

Abstract

This study aimed to analyze the effect of green grass jelly leaf extract addition on the physicochemical and organoleptic properties of panna cotta. The research employed a laboratory experimental method using a Completely Randomized Design (CRD) with one factor, namely the combination of green grass jelly leaf extract and UHT milk concentrations at four treatment levels: P1 (20% green grass jelly leaf extract and 50% UHT milk), P2 (25% and 45%), P3 (30% and 40%), and P4 (35% and 35%). The evaluated parameters included Texture Profile Analysis (TPA), protein content, pectin content, and organoleptic assessment covering color, aroma, taste, and mouthfeel. The effectiveness test results indicated that treatment P1 produced the best overall quality with a Result Value (RV) of 0.55562. This treatment was characterized by balanced textural properties, protein content of 5.66%, pectin content of 8.92%, and good panelist acceptance, particularly in taste and mouthfeel attributes. In conclusion, green grass jelly leaf extract has strong potential as a natural gelling agent in panna cotta production, especially at a concentration of 20%, offering a practical local-based alternative to gelatin.
Pengaruh Pemberian Ekstrak Buah Gandaria (Bouea macrophylla Griff) Terhadap Perubahan Ekspresi Gen CD36 Pada Otak Tikus Yang Diinduksi CCL4 (Karbon Tetraklorida) Wahyuni, Rana; Idami, Zahratul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19356

Abstract

This study aimed to analyze the effect of gandaria fruit extract (Bouea macrophylla Griff.) on CD36 gene expression in the brains of CCl₄-induced rats (Rattus norvegicus). The experiment employed a completely randomized design consisting of five groups: negative control, positive control (CCl₄), and three treatment groups administered gandaria fruit extract at doses of 100, 150, and 200 mg/kgBW. CD36 gene expression was assessed using semi-quantitative PCR, normalized to the GAPDH reference gene, and analyzed based on band density using ImageJ software. Statistical analysis was performed using one-way ANOVA followed by post hoc testing at a significance level of p < 0.05. The results demonstrated that CCl₄ induction increased CD36 expression by 1.9-fold compared to the control group. Administration of gandaria fruit extract reduced CD36 expression relative to the CCl₄ group, with the lowest fold change observed at 100 mg/kgBW (1.08), while higher doses showed relatively increased expression but remained lower than the positive control. These findings indicate that gandaria fruit extract has the potential to modulate CD36 gene expression under CCl₄-induced oxidative stress conditions, with the most effective response observed at the lowest dose.
Kinerja Fitoremediasi Typha angustifolia L dalam Menurunkan Konsentrasi Timbal dan Tembaga di Sungai Silugonggo Juwana Lestari, Safira Asanu; Ulfah, Maria; Dewi, Endah Rita Sulistiya
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19383

Abstract

This study aimed to evaluate the effectiveness of Typha angustifolia L. as a phytoremediation agent in reducing Pb and Cu concentrations in river water. The research employed a true experimental method using a completely randomized design with three biomass treatments (0 g, 80 g, and 160 g), each conducted in triplicate at two independently treated observation stations. The incubation period lasted seven days, during which metal concentrations were measured on days 0, 3, and 7 using atomic absorption spectrophotometry (AAS). The results demonstrated that the 160 g biomass treatment achieved the highest remediation efficiency. On day 7, Pb concentrations were reduced by 98.87% at Station 1 and 98.13% at Station 2, while Cu concentrations decreased by 91.76% and 94.88% at Stations 1 and 2, respectively. Statistical analysis using ANOVA followed by Tukey’s post hoc test indicated a significant effect of treatment on metal reduction (p < 0.05). It can be concluded that Typha angustifolia L. is effective as a phytoremediation agent, with remediation efficiency increasing with greater biomass and longer contact time, highlighting its potential application as an environmentally friendly solution for heavy metal pollution control.
Pengaruh Tingkat Kecemasan Terhadap Kadar Gula Darah Pada Penderita Diabetes Melitus Tipe 2 Fardiman, Bambang; Saimi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.16501

Abstract

People with diabetes mellitus experience anxiety because the condition is considered a scary disease that has complex adverse effects on their anxiety levels. Uncontrolled diabetes often results in increased blood sugar levels. The chronic condition known as diabetes mellitus is caused by insufficient insulin production by the pancreas or inefficient use of insulin by the body. The purpose of this study was to determine how anxiety affects blood sugar levels in patients with diabetes mellitus at the Praya Health Center. This type of research is a quantitative study with a cross-sectional approach. The research sample of 140 respondents was determined by Random Sampling with the Zung Self-Rating Anxiety Scale (ZSAS) Questionnaire, an instrument used to measure a person's anxiety level. Based on the results of the study, the level of anxiety on blood sugar levels has a significant effect with a linier regeration statistical test obtained a coefficient value indicating that if the anxiety value increases by 1 unit, then the GDS will increase by 1.594 units, which means there is an influence and a significant value of 0.001 < (0.005) between the level of anxiety and blood sugar levels in patients with type 2 DM at the Praya Health Center
Uji Aktivitas Klorofil Daun Putri Malu (Mimosa pudica Linn) Sebagai Antibakteri Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Rahmah, Annisa; Idramsa; Ritonga, Iin Agustina
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18470

Abstract

Infectious diseases caused by pathogenic bacteria such as Staphylococcus aureus and Escherichia coli remain a health problem, especially due to increasing antibiotic resistance. Therefore, antibacterial alternatives from natural materials are needed, one of which is the chlorophyll pigment from Mimosa pudica Linn. This study aims to test the antibacterial activity of chlorophyll from young and old leaves of M. pudica Linn and compare the inhibitory power of both against test bacteria. This study is an experimental study that includes the preparation of simple drugs, ethanol extraction, liquid-liquid fractionation, purification using thin layer chromatography (TLC) and column chromatography, and antibacterial testing by disc diffusion method using standard bacterial inoculum McFarland 0.5. The purification results showed an Rf value of 0.87 with one spot indicating pure chlorophyll fraction. The antibacterial activity of chlorophyll is relatively weak, but the chlorophyll fraction of old leaves showed greater inhibitory power than young leaves. The highest inhibition zone against S. aureus was obtained from old leaf chlorophyll of 4.00 ± 1.01 mm, while against E. coli it was 2.54 ± 0.52 mm.
Potential of Basil Seed (Ocimum basilicum) Simplicia as a Feed Supplement on the Histomorphometry and Muscle Damage of African Catfish (Clarias gariepinus) Exposed to Polystyrene Microplastics Iga Novianti; Nurul Suwartiningsih
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19297

Abstract

This study aimed to evaluate the potential of basil seed simplicia supplementation in feed to mitigate histomorphometric alterations and muscle damage in African catfish (Clarias gariepinus) exposed to polystyrene (PS) microplastics. The experiment employed a Completely Randomized Design (CRD) consisting of five treatments: C (0 mg/L PS microplastics + 0% basil seed simplicia), PC (0 mg/L PS microplastics + 2% basil seed simplicia), NC (100 mg/L PS microplastics + 0% basil seed simplicia), T1 (100 mg/L PS microplastics + 2% basil seed simplicia), and T2 (100 mg/L PS microplastics + 4% basil seed simplicia). A total of 125 catfish were exposed to microplastics for 15 days. The observed parameters included myofiber diameter, myofiber area, muscle degeneration, and necrosis. The results indicated that exposure to PS microplastics significantly decreased myofiber diameter and area while increasing the levels of muscle cell degeneration and necrosis (P < 0.05). Supplementation with 2% basil seed simplicia (T1) significantly improved myofiber diameter and area and reduced muscle degeneration and necrosis compared with the negative control (NC), whereas supplementation with 4% basil seed simplicia (T2) showed relatively lower effectiveness. These findings suggest that supplementation of 2% basil seed simplicia in feed has the potential to mitigate histomorphometric alterations and muscle tissue damage in catfish exposed to polystyrene microplastics.
Pengembangan Ensiklopedia Keanekaragaman Hewan Vertebrata dan Invertebrata di Danau Lau Kawar Sebagai Sumber Belajar Untuk Meningkatkan Kemampuan Literasi Sains Mahasiswa Sitanggang, Monica; Sinambela, Masdiana; Arwita, Widya
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19317

Abstract

The purpose of this study was to produce an encyclopedia of vertebrate and invertebrate animal diversity in Lake Lau Kawar that is valid, feasible, and effective as a biology learning resource based on scientific literacy. The low scientific literacy of students and the limited contextual biology learning resources based on local potential are problems in learning. This study used the ADDIE development model method which includes the stages of analysis, design, development, implementation, and evaluation. The subjects of the study were students of the Biology Education Study Program in the Basic Ecology course. From the results of the research data processing, the results of the ADDIE development procedure were obtained, the validity of the material expert product was an average of 90.15% (very good), the validity of the design expert product was an average of 95.44% (very good), the validity of the learning expert product was an average of 88.86 (very good), the average lecturer response was 89.68% (very good), and the results of the N-Gain analysis of the results showed that the average value of the experimental class was higher than the control class. The results showed that the developed encyclopedia was in the very feasible category based on expert assessments and received very positive responses from users. Effectiveness testing showed an increase in students' scientific literacy skills, ranging from moderate to high. Therefore, this locally-based encyclopedia of animal diversity is suitable and effective as a biology learning resource in higher education.
Pengembangan E-Modul Berbasis SETS (Science, Environment, Technology, and Society) Pada Materi Inovasi Teknologi Biologi untuk Meningkatkan Kemampuan Berpikir Kritis Siswa Kelas X SMA/MA Zahrah, Thifal; Idramsa; Lubis, Khairiza
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19381

Abstract

This study aims to describe the validity, teacher responses, student responses, practicality, effectiveness, and the improvement of critical thinking skills through the development of a SETS-based. This study employed a Research and Development (R&D) approach using the 4D model. The results show that: (1) The validity of the SETS-based e-module is categorized as very feasible, with an average score of 85% for material validation, 95% for media validation, and 89% for language validation, indicating that the e-module is suitable for instructional use. (2) Teacher responses indicate that the SETS-based e-module helps students develop more comprehensive and contextual thinking, the average score obtained for the model design aspect was 85%, the concept mastery aspect was 90% and the learning motivation aspect was 88%. (3) Student responses demonstrate that the e-module meets the criteria of practicality, comfort, and usefulness, and successfully enhances learning interest and motivation. The results obtained for the module design aspect were 95%, the concept mastery aspect was 89% and the learning motivation aspect was 90%. (4) The practicality and effectiveness tests reveal an average score of 91%, placing the e-module in the highly practical and highly effective categories for learning. (5) The improvement of students’ critical thinking skills is evidenced by the t-test 0.05, which indicates a significant difference between pretest and posttest scores. The average N-Gain score of 0.758, categorized as high, shows that the SETS-based e-module increased students’ critical thinking ability by 76%. Overall, the developed SETS-based e-module is valid, practical, effective, and capable of significantly improving students’ critical thinking skills.
Potensi Karbon dan Nilai Jasa Lingkungan di Agroforestri LPHN (Lembaga Pengelola Hutan Nagari) Sungai Buluh, Padang Pariaman, Sumatera Barat Rahmawati, Lily; Chairul; Solfiyeni
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19456

Abstract

This study aims to analyze biomass quantity, carbon reserves, carbon sequestration, and to determine the environmental value of carbon sequestration in the agroforestry area of LPHN Sungai Buluh Padang Pariaman. This is because agroforestry areas can also play a role in carbon sequestration in addition to their economic function for the community. This study was conducted using purposive sampling to determine the location of 30 observation plots. Data collection was carried out using destructive sampling for undergrowth and litter data and non-destructive sampling for tree and sapling strata data. The results of this study were a total biomass of 280.768 tons/ha, total carbon reserves obtained from the biomass value of 131.955 tons/ha, and total carbon sequestration of 484.294 tons/ha. Meanwhile, the environmental service value of carbon sequestration was IDR 82,566,423,881.28/ton/year. The environmental service value obtained from this research location is relatively low compared to studies conducted in other locations, primarily due to the limited vegetation types and small study area.

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