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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,428 Documents
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The Characteristics of the Thinking Scale of Class X Students on the Water Cycle Material Safitri, Devi Oktaviani; Wicaksono, Azizul Ghofar Candra; Prasetiyo
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19698

Abstract

This study aims to describe the characteristics of the systems thinking abilities of Class X students in the water cycle material across the cognitive and affective domains. The research uses a quantitative descriptive approach with a survey method. The research  sample consis of 80 Class X students from SMA Negeri 10 Semarang, selected through purposive sampling. The research instruments included a multiple-choice test to measure systems thinking abilities in the cognitive domain and attitude questionnaires to measure the affective domain. The instruments were developed based on a hierarchical systems thinking framework and a systems thinking scale. The data were analyzed descriptively and tested using the Pearson Product Moment correlation. The results show that the cognitive domain systems thinking abilities are in the high category, particularly at the analysis level, where more than 70% of students reached the high category, while the synthesis and implementation levels are in the moderate category. The affective systems thinking abilities are generally in the moderate category, with percentages ranging from 52.50% to 81.25%. There is a very strong and significant relationship between the cognitive and affective domains at the same level is considered low to moderate.These findings highlight the need for more context-rich integrative learning to optimize students’ systems thinking abilities.
Degradation of Mangrove Ecosystems in Banyuasin Regency, South Sumatra: Extent of Damage, Causes, and Ecological Impacts Mahmudi, Ali; Bakri; Rozirwan; Taqwa, Ridhah
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19713

Abstract

This study aims to examine the scale of damage, identify the contributing factors, and analyze the resulting ecological impacts. The research employed a qualitative descriptive approach through field observations, interviews with coastal communities, and a literature review. Observations were conducted to assess the biophysical condition of mangroves, including vegetation density, stand structure, species composition, and indicators of degradation. Interviews were used to gather information on mangrove utilization activities, socio-economic pressures, and community perceptions of environmental change. The literature review supported the interpretation of field findings within the context of regional and global mangrove dynamics. The results indicate that mangrove degradation in Banyuasin is driven by a complex interaction between anthropogenic factors—particularly land conversion, resource exploitation, and coastal development—and natural factors such as abrasion, sedimentation, and tidal flooding. The identified ecological impacts include a decline in coastal protection functions, reduced habitat quality for coastal biota, and decreased carbon storage capacity. The conclusion of this study emphasizes the need for an integrated coastal management strategy that incorporates ecologically appropriate rehabilitation, strengthened governance, and community participation in order to sustainably enhance the resilience of mangrove ecosystems.
Potential of the Fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) as an Endophytic Fungus for the Growth of Yardlong Bean (Vigna sinensis L.) Naufal, Muhammad Raihan; Kusuma, Ramadhani Mahendra; Purnawati, Arika
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19725

Abstract

The main objective was to determine the effectiveness of this technique in establishing endophytic colonies and its impact on plant vegetative growth. The experiment was conducted in the laboratory and greenhouse using a Completely Randomized Design (CRD) approach. We tested four levels of conidial density: control (no conidia), 1×10⁶, 1×10⁷, and 1×10⁸ conidia per mL, with each replicated five times. Parameters we observed included root and stem colonization success, plant height, and leaf number. Data were analyzed using ANOVA followed by a HSD test at the 5% level. The results showed that B. bassiana is able to enter and develop internally within root and stem tissues. Interestingly, the highest colonization occurred at the lowest concentration (1×10⁶ conidia per mL), with 83.3% in roots and 66.7% in stems. Although statistically, differences in concentration did not always have a significant effect on plant height and leaf number, numerically, inoculated plants showed better growth than controls, especially at 28 days after planting. From these findings, we conclude that soil application of B. bassiana is quite effective in establishing endophytic colonies without disrupting vegetative growth. In other words, this method is worth considering as part of a more environmentally friendly and sustainable pest management strategy.
The Effectiveness of an Environment-Based Biodiversity Flipbook E-Module on Students’ Biology Understanding and Scientific Process Skills in Grade X of SMA Negeri 21 Medan Rumapea, Lusiana; Sinambela, Masdiana; Silitonga, Melva
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19762

Abstract

This study aimed to analyze the effectiveness of an environment-based Biodiversity Flipbook E-Module in improving tenth-grade students’ understanding of biology and their scientific process skills at SMA Negeri 21 Medan. The study employed a one-group pretest–posttest design involving 36 students. Research instruments included a validated biology comprehension test and an observation sheet used to assess students’ scientific process skills during three environment-based learning sessions. Data were analyzed using the N-gain score and descriptive statistics. The results showed that the mean pretest score of 33.81 increased to 83.88 in the posttest, resulting in an N-gain score of 0.76, which falls into the high category. Students’ scientific process skills were classified as very good across all learning sessions and demonstrated an increasing trend during the final meeting. These findings indicate that the environment-based Biodiversity Flipbook E-Module is effective in enhancing students’ understanding of biological concepts and their scientific process skills through contextual learning grounded in real environmental exploration.
Evaluation of a Herbal Toothpaste Formulation with the Addition of Nginang Ingredients as an Antimicrobial Agent Against Streptococcus mutans Ibrahim, Khazari Noorin Husna; Rasyidah
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19796

Abstract

This study aimed to determine the antimicrobial activity of herbal toothpaste formulations containing betel leaf (Piper betle L.) extract against Streptococcus mutans and to evaluate the physical characteristics of the resulting preparations. The research employed a laboratory experimental design with variations in betel leaf extract concentrations of 5%, 10%, and 15%, each conducted in four replicates. The analyses included phytochemical screening, antimicrobial activity testing using the disc diffusion method, and evaluation of the toothpaste preparations, including pH measurement, organoleptic assessment, homogeneity testing, foam formation analysis, and viscosity determination. The results of the phytochemical screening indicated that the betel leaf extract positively contained triterpenoids, flavonoids, saponins, phenolic compounds, and alkaloids. The antimicrobial activity test demonstrated that the betel leaf extract produced average inhibition zones of 16.4 mm, 22.7 mm, and 25.6 mm at concentrations of 5%, 10%, and 15%, respectively. The evaluation of the toothpaste formulations showed pH values ranging from 8.04 to 8.42, which fall within the Indonesian National Standard (SNI) range of 4.5–10.5. The preparations exhibited a semi-solid and homogeneous texture, with the highest foam height of 20.6 mm observed at the 15% extract concentration, and viscosity values within the required acceptable range. In conclusion, the herbal toothpaste formulation containing betel leaf extract demonstrated strong antimicrobial activity against Streptococcus mutans and met the required physical parameters for toothpaste preparations. Therefore, it has potential to be developed as an alternative herbal toothpaste based on traditional natural ingredients suitable for oral health applications.
Development of an Atlas of Mangrove Plant Morphology Based on the Local Potential of PPLH Puntondo Takalar as a Supplementary Teaching Material for the Plantae Topic in Grade 10 Senior High School Akma, Nurul; Hasanah, Ummul; Ibrahim, Misykat Malik
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19095

Abstract

This study aimed to develop a mangrove plant morphology atlas based on the local potential of the Puntondo Takalar Center for Environmental Education (PPLH Puntondo Takalar) as a supplementary teaching material for the Grade 10 high school Plantae topic that meets the criteria of validity, practicality, and effectiveness. The study employed a Research and Development (R&D) method using the ADDIE model, which consists of the analysis, design, development, implementation, and evaluation stages. The research subjects comprised 26 Grade 10 students and one biology teacher in a limited trial conducted at a senior high school in Takalar Regency. Data were collected through observation, interviews, needs analysis questionnaires, expert validation sheets, response questionnaires, and learning outcome tests. The results showed that the developed atlas included nine mangrove species identified through field observations, presented systematically based on morphological characteristics and distribution locations. The atlas was supplemented with field photographs, identification keys, distribution maps, and observation tasks that support environment-based learning and scientific literacy. Expert validation results yielded an average score of 3.6, categorized as highly valid. The practicality test indicated a positive response rate of 92% from both the teacher and students, categorized as highly practical. The effectiveness test showed that classical learning mastery reached 100%, with all students achieving scores above the minimum mastery criterion (KKM). Therefore, this mangrove plant morphology atlas based on local potential is feasible for use as a supplementary teaching material and has the potential to support contextual biology learning while enhancing students’ biodiversity literacy.
Morphometric Description of Polistes tepidus from Ternate Island and Obi Island (Wallacea, Indonesia) Bintoro, Wahyu Agung; Nugroho, Hari; Purwatiningsih
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19639

Abstract

This study aimed to descriptively compare the morphometric measurements of two Polistes tepidus specimens from Ternate Island and Obi Island preserved in the Museum Zoologicum Bogoriense. The research was conducted by measuring morphometric characters using a Leica Z6 APO microscope and L.A.S. 4.13 software. The observed characters included the head, thorax, wings, and metasoma, which were measured from three viewing angles: frontal, lateral, and dorsal. A total of 33 morphometric characters of the paper wasp were examined, and the mean measurements were analyzed using Microsoft Excel. The results showed that the morphometric measurements of P. tepidus from the two different locations, Ternate Island and Obi Island, exhibited size variation in several body characters. In general, P. tepidus individuals from Ternate Island had larger body sizes than those from Obi Island for most morphometric characters. Habitats with abundant food resources may allow individuals to develop larger body sizes. The morphometric differences between P. tepidus from Ternate and Obi Islands may also be explained by the effects of geographic isolation and the differing ecological conditions of the two islands. The islands of the Wallacea region are known for their high environmental heterogeneity, and insect populations therefore often exhibit morphological differences among regions. These findings highlight the need for broader sampling to further test geographic differentiation.
Effect of Variations in Blood Component Supplementation on the Suitability of Blood Agar Plate Media for the Growth of Salmonella typhi Setyoningrum, Fransisca Probo
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19748

Abstract

This study aimed to evaluate the effect of different human blood components added to Blood Agar Plate (BAP) media on the growth of Salmonella typhi O. An experimental design was employed with three treatment groups and a total of 27 replicates. Salmonella typhi O was inoculated onto BAP media containing different blood components, followed by incubation at 37°C for 24 hours. Bacterial colonies were then counted using the total plate count method. Statistical analysis was performed using the Shapiro–Wilk test and one-way ANOVA, followed by Bonferroni post hoc analysis. The results showed that variation in blood components significantly affected colony counts (p = 0.0001). Whole blood containing antibodies produced the lowest bacterial growth (mean 2.6 × 10⁵ CFU/mL), whereas erythrocyte suspension (1.8 × 10⁶ CFU/mL) and whole blood without antibodies (1.9 × 10⁶ CFU/mL) showed substantially higher growth and did not differ significantly from each other (p = 0.618). These findings indicate that antibodies in human blood can inhibit the growth of Salmonella typhi through complement activation, while washed erythrocytes are effective as a substitute for sheep blood. Therefore, whole blood without antibodies or erythrocyte suspension may serve as viable and effective alternatives for the preparation of BAP media in laboratories with limited access to sheep blood.
The Use of PlantNet Identify for Identifying Arecaceae Family Plants in Biology Learning Among Biology Education Students at Universitas Labuhanbatu Simbolon, Meliani; Khairul; Sari, Novi Fitriandika
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19758

Abstract

This study aimed to examine the use of the PlantNet Identify application in identifying higher plants of the Arecaceae family in biology learning among Biology Education students at Universitas Labuhanbatu. The study employed a descriptive quantitative approach, involving 21 Biology Education students selected using a total sampling technique. The data were primary data collected through an online questionnaire distributed via Google Forms, with the use of the PlantNet Identify application as the independent variable (X) and the identification of higher plants of the Arecaceae family as the dependent variable (Y). The results of the simple linear regression analysis indicated no significant relationship between the use of the PlantNet Identify application and students’ ability to identify higher plants of the Arecaceae family, with a significance value of 0.357 (p > 0.05). The R² value further showed that the application contributed only 4.48% to students’ identification ability. These findings indicate that there is no strong correlation between the use of PlantNet Identify and students’ ability to understand plants belonging to the Arecaceae family. However, the results of this study are limited by the small sample size.
Density of Clams (Polymesoda sp.) in the Mangrove Ecosystem of Pinotu Village, Toribulu District, Parigi Moutong Regency Niwana, Nuning; Masrianih; Sabran, Moh.; Sutrisnawati; Zainal, Syech; Dhafir, Fatmah
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19828

Abstract

This study aimed to assess the density of Polymesoda sp. in the mangrove ecosystem of Pinotu Village, Toribulu District, Parigi Moutong Regency, an area experiencing habitat pressure due to environmental change. A quantitative descriptive approach was applied using purposive sampling across three stations: Station 1 behind the residential area, Station 2 in the central mangrove zone, and Station 3 near shrimp ponds. Sampling was conducted at low tide using a line transect method with zigzag plots. Each station consisted of three 25 m transects spaced 10 m apart, with five 1 m² plots established along each transect, yielding a total of 45 observation plots. Environmental parameters measured included temperature, salinity, and pH. The highest density of Polymesoda sp. was recorded at Station 2 (2.93 ind/m²), followed by Station 1 (1.87 ind/m²), whereas the lowest density was observed at Station 3 (0.87 ind/m²). Differences in density were associated with habitat variation, particularly mangrove vegetation density, substrate characteristics, and environmental physical and chemical conditions. These findings indicate that more stable and better-preserved mangrove habitats support higher densities of Polymesoda sp., highlighting the importance of habitat quality in sustaining its population.

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