cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Ivandini Tribidasari A.
Contact Email
ivandini.tri@sci.ui.ac.id
Phone
+622129120943
Journal Mail Official
editor_mss@ui.ac.id
Editorial Address
Directorate of Research and Community Engagement UNIVERSITAS INDONESIA UI Campus, Depok 16424 Indonesia
Location
Kota depok,
Jawa barat
INDONESIA
Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
Arjuna Subject : -
Articles 10 Documents
 1 
Search results for , issue "Vol. 28, No. 2" : 10 Documents clear
Microencapsulation of Cosmos caudatus Kunth Extract using Sodium Alginate and In Vitro Antioxidant Activity Test Fadlila, Safina Samara Nur; Puspita, Oktavia Eka; Ningsih, Zubaidah; Safitri, Anna
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to encapsulate Cosmos caudatus K. leaf extract in sodium alginate crosslinked with calcium chloride (CaCl2) as coating materials through freeze-drying. Antioxidant assays were performed to evaluate the applicability of the microcapsules as antidiabetic medicines. Their characteristics, antioxidant activity, and encapsulation efficiency (EE) were also examined. Results indicated that the concentration of sodium alginate and pH influenced the manufacturing process of the microcapsules. The highest EE was obtained at pH 6 and alginate concentration of 2% (w/v). The IC50 for the antioxidant activity of the microcapsules was 139.96 ± 1.094 μg/mL. Scanning electron microscopy confirmed the presence of irregular and spherical structures on the surface. Fourier transform infrared spectra showed new absorption bands at 1593.10 and 1427.59 cm−1, demexistening the existence of stretching vibrations of COO−. Absorption at 1024.14 cm−1 demonstrated C–C and C–O bond stretching vibrations in the sodium alginate–CaCl2 crosslinks. Fourier transform infrared spectrum analyses indicated that sodium alginate and CaCl2 formed chemical bonds, enabling microencapsulation. This study discovered that microencapsulation is a highly prospective and adaptable strategy for enhancing the medicinal use of plant extract.
BIO-SYNTHESIS OF COPPER NANOPARTICLES FROM INDIGOFERA TINCTORIA LEAF Kayode, Talabi Henry; Adewale, Esther Dolapo; Adewuyi, Benjamin Omotayo
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Leaf extracts are known to be rich in phytoconstituent biomolecules, making them a valuable source of medicinal compounds. They also serve as both capping and reducing agents in nanoparticle fabrication. A reaction between CuSO4.5H2O aqueous solution and the Indigofera tinctoria leaf extract results in the formation of stable copper nanoparticles. Phytochemical screening of the Indigofera tinctoria leaf extract revealed the presence of various compounds including carbohydrates, terpenoids, phenol, tannins, flavonoids, saponins, and glycosides in the sample. The biosynthesized copper nanoparticles (CuNPs) were subsequently subjected to various forms of analysis. Techniques used included UV–visible (UV–vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy. An observable change in the color of solutions from pale to thick brown indicated the formation of CuNPs. Further confirmation came from UV–vis spectroscopy, which established the production of CuNPs at 500 nm. FTIR analysis revealed that the CuNPs were covered by organic residues. The particles ranged from 210 nm to 260 nm as indicated from the SEM analysis.
Synthesis, Biological Activity, and Molecular Docking Study of Xanthenol and Its Disproportionation Products as Anticancer and Antimalarial Agents Pratama, Ardhya Ayu; Jumina, Jumina; Anwar, Chairil
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Xanthone and its derivatives are well known for their broad biological activities. This research aims to investigate the anticancer and antimalarial activities of synthesized xanthenol and xanthene compounds and to elucidate their mechanisms of action through molecular docking. The xanthenol compound was obtained through xanthone reduction with sodium triacetoxyborohydride, and xanthene synthesis was studied through three different reaction conditions: uncatalyzed and catalyzed by Lewis acid or Bronsted acid. The reduction reaction produced xanthenol in 30.50% yield, whereas the three reaction conditions produced xanthene and xanthone in 39.35–75.48% yield. An anticancer assay for Vero, WiDr, HeLa, and T47D cell lines was evaluated with a microculture tetrazolium assay, and an antimalarial activity test was examined using the heme polymerization inhibition method. The xanthene compound showed the lowest IC50 value (44 µg/mL) among the products in the T47D cell line. Meanwhile, the antimalarial assay showed that the xanthone compound could inhibit heme polymerization, with an IC50 value of 114 µg/mL. The molecular docking study revealed that the anticancer activity of xanthene occurred through the inhibition of the cyclooxygenase-2 (COX-2) enzyme, and the antimalarial activity of xanthone occurred through the inhibition of the Plasmodium falciparum lactate dehydrogenase enzyme. These results showed that xanthene and xanthone compounds are potential anticancer and antimalarial drugs, respectively.
Limonoids Isolated from Chisocheton pentandrus (Meliaceae) Stembarks and its Cytotoxicity Towards MCF-7 Breast Cancer Cell Line Runadi, Dudi; Anjari, Intan Hawina; Purnama, Purnama; Nurlelasari, Nurlelasari; Harneti, Desi; Mayanti, Tri; Harizon, Harizon; Hidayat, Ace Tatang; Salam, Supriatno; Azmi, Mohamad Nurul; Supratman, Unang
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chisocheton is a large source of limonoids with various pharmacological effects, such as antiviral, antibacterial, antimalarial, antifungal, and cytotoxic activities. This study aimed to isolate, elucidate, and evaluate the cytotoxicity of limonoids from C. pentandrus stembarks. Isolation was carried out using various separation methods including extraction and column chromatography. Spectral data were analyzed by FT-IR, UV, DEPT 135°, 1H, 13C-NMR, and HRTOF-MS and compared with those previously reported to determine the chemical structure.The obtained limonoids were lasiocarpine (1), lasiocarpine B (2), pentandricine (3), and 16β-hydroxydysobinin (4); all of which were successfully isolated from C. pentandrus for the first time. The cytotoxic activity of these limonoids were also evaluated against Michigan Cancer Foundation-7 (MCF-7) breast cancer cells using PrestoBlue method. Lasiocarpine A revealed the strongest cytotoxicity with an IC50 of 42.62 µM in moderate level.
Molecular Docking of the Interaction between Citrus amblycarpa Extract Contents and Inflammatory Proteins of Hepatic Steatosis Rosida, Lena; Pratiwi, Dewi Indah Noviana; Panghiyangani, Roselina; Utami, Juliyatin Putri; Idhafi, Nasrul; Febriansyah, Muhammad; Budiarman, Andi Azizah Maulidia
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Citrus amblycarpa possesses various pharmacological activities, such as antioxidant, anticancer, antitumor, hepatoprotective, anti-inflammatory, antidiabetic, antiviral, antibacterial, and antifungal. The main active compounds in C. amblycarpa (including gamma (γ)-aminobutyric acid [GABA], hesperidin, naringin, neoeriocitrin, poncirin, quercetin, and rutin) show potential to interact with the inflammatory proteins in hepatic steatosis (such as nuclear factor kappa beta [NF-kB], tumor necrosis alpha [TNF-alpha], interleukin-6 [IL-6], c-Jun NH2-terminal kinase [JNK], and adiponectin). Molecular docking simulations were performed using Swiss Dock (http://www.swissdock.ch/), and analysis and visualization were conducted using Discovery Studio 4.1. Rutin, poncirin, hesperidin, and neoeriocitrin exhibit high affinities to NF-κB, TNF-alpha, IL-6, and adiponectin proteins, respectively. Similar to curcumin–adiponectin complex interaction, neoeriocitrin–adiponectin interaction involves GLY 223, PRO41, and VAL93 residues. Thus, the most potent inhibitor of hepatic sterosis marker was neoeriocitrin.
Association between Macrophage’s Cell Number and Maternal Factors in Human Milk Khudri, Ghaniyyatul; Sukmawati, Dewi; Barasila, Atikah Chalida; Suryandari, Dwi Anita
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infant nutrition and immune protection require the consumption of human milk, with macrophages mainly contributing to immune defense and infant development. This study was performed to investigate the correlation between macrophage cell numbers in human milk and various maternal factors. Thirteen human milk samples were collected from lactating mothers and were evaluated for macrophage cell numbers using a hemacytometer. The recorded questionnaire containing several questions, including age, multivitamin consumption, coronavirus disease 2019 vaccination status, allergy history, and lactation duration, was used to determine the maternal factors. Pearson’s correlation and Mann-Whitney test was employed in statistical analysis, with p-values < 0.05 considered significant. The macrophage cell number reached 54.236 ± 7.456 cells per mL. Notably, a significant correlation was observed between maternal allergy history and the number of macrophage cells in human milk (p = 0.049). Meanwhile, no statistically significant associations were detected among multivitamin consumption (p = 0.833), vaccination status (p = 0.923), and lactation duration (p = 0.236). This study emphasizes the effect of maternal characteristics on the composition and immune properties of human milk. The specific mechanisms underlying these correlations and their potential influence on infant health and development must be explored in future research.
Benefits of Plant Pigment, Quercetin, for Fish and Prawn Prakoso, Yos Adi; Hidayah, Jasir Hakim; Sanjaya, Michael Richard; Pratama, Agga Dhigayuka; Azhar, Annisha Permata Nurul; Kuswanto, Arya Surya; April, Berlian Ronaldi; Utami, Intan Karisma; Putra, Mahera Sandi Sasmita; Wukak, Maria Paulina; Satria, Naufal Farros
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Quercetin is an important phytochemical that belongs to the flavonoid group of plant pigments. It has potential benefits as an antioxidant, antiviral, and anti-inflammatory agent. However, the utilization of quercetin in aquaculture is not optimal. Hence, this study conducted a literature review to analyze the potential benefits of quercetin in aquaculture, especially for fish and prawn. Results showed that quercetin can be isolated from herbs using several methods, including sonication, maceration, and fractionation. Its amount in plants can be potentially determined using high-performance liquid chromatography and thin-layer chromatography. Some of the plants containing high quercetin levels include shallot, garlic, apple skin, guava leaves, green tea, chia seeds, and desert rose flower. In aquaculture, quercetin can be applied to increase the ideal body weight, feed conversion ratio, and condition factor of aquatic cultured species and serves as an antioxidant, antistress, strengthening probiotic and an antiinfective agent. Latest research showed that quercetin benefits the immunity of goldfish against furunculosis. Furthermore, quercetin shows potential in prawn industry as an antioxidant protection that improves the meat quality. To date, the potential benefits of quercetin have not been widely utilized in aquaculture. The minimum utilization can be attributed to the lack of a systematic review regarding its potency either on fish or prawn. Hence, this study provides the essential review of quercetin in aquaculture.
Degradation of Chlorpyrifos Residue on Tropical Inceptisol Soil with Indigenous Bacteria Treatment Kurnia, Asep; Zu`amah, Hidayatuz; Sulastri, Baiq Nunung; Makmur, Ria Fauriah
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The use of soil microbes is one of the most effective methods for degrading pesticide residue in soil. Microbes can accelerate the degradation of persistent pollutants including chlorpyrifos in soil. This research aims to find soil bacteria with the greatest potential to degrade chlorpyrifos residue in tropical inceptisol soil and determine its degradation index (DI). The soil used in this experiment was collected from agricultural fields in the districts of Demak, Central Java Province, Indonesia. The experiment was performed using factorial randomize complete block design composed of two factors and four replications. The first factors were made up of two treatments, namely, sterile soil (S) and nonsterile soil (NS). The second factors were composed of five treatments, namely, 1) Delftia acidovorans, 2) Entrobacter sp., 3) Chryseobacterium geocarposphaerae, 4) a mix of three bacteria, and 5) without bacteria (check). Results revealed that D. acidovorans grew faster than other bacteria in chlorpyrifos-contaminated soil. The optimum condition of bacteria growth and chlorpyrifos degradation was observed at the temperature of 28.9 ℃ and soil pH of 5.75. D. acidovorans promoted the degradation of chlorpyrifos residue in soil faster than others with a DI of 0.096. The half-life of chlorpyrifos in soil was faster in NS than in S.
Optimizing the Expression of Polyethylene Terephtalate Hydrolase-Encoding Synthetic Gene in Escherichia coli Arctic Express (DE3) Nataniel, Jocelyn; Ulfah, Maria; Achnafani, Dini; Nurhayati, Niknik; Sabbathini, Gabriela Christy; Wulandari, Sri Rezeki; Abinawanto, Abinawanto; Helianti, Is
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The waste of polyethylene terephthalate (PET) plastic waste in Indonesia is a pressing concern due to its slow degradation and potential environmental damage. One promising solution is to utilize polyethylene terephthalate hydrolase from Ideonella sakaiensis (IsPETase), an enzyme that specifically degrades PET. However, inducing the expression of IsPETase synthetic gene in Escherichia coli BL21 (DE3) has been challenging because much of it remains insoluble. This study aimed to express IsPETase in E. coli Arctic Express (DE3) and optimize the conditions to enhance its production. First, pET22b(+)pelB-IsPETase was inserted into E. coli Arctic Express (DE3). The recombinant E. coli Arctic Express (DE3) was induced with isopropyl-β-D-1-thiogalactopyranoside (IPTG) and incubated at 10 °C. The fraction expressing soluble IsPETase was determined in different culture media, IPTG concentrations, induction times, and soni-cation durations. Parameters were optimized using a one-factor-at-a-time approach and then evaluated based on esterase specific activity and SDS-PAGE analysis. Results showed that IsPETase can be expressed in extracellular, periplasmic, and cytoplasmic soluble fractions. However, the extracellular fraction should be concentrated. Subsequent optimization focused only on the cytoplasmic fraction under optimal conditions, achieving a threefold increase in PETase specific activity compared with that under uninduced IPTG conditions. The reaction of PETase enzyme with PET and PCL was proven by weight loss, Scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FTIR). Although successful IsPETase expression and production optimization have been achieved, the specific activity remains low, prompting the need for ongoing expression optimization.
Preparation of β-Ni (OH)2 Nanosheet–Modified Glassy Carbon for Pseudocapacitors Putra, Muhammad Buchari Setia; Aliyah, Aliyah; Sanjaya, Afiten Rahmin; Pramadewandaru, Respati Kevin; Chung, Hoeil; Ivandini, Tribidasari Anggraningrum
Makara Journal of Science Vol. 28, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

β-Nickel hydroxide (Ni(OH)2) nanosheet–modified glassy carbon for pseudocapacitors was successfully prepared. The nanosheets were synthesized via a conventional growth seeding method combined with hydrothermal treatment. Transmission electron microscopy (TEM) characterization indicated the hexagonal structure of the nanosheets of around 15 nm in size, and X-ray diffraction (XRD) pattern confirmed the β-phase lattice crystal. These nanosheets used to modify the glassy carbon surface, increased the electroactive surface area by around 15 times. Furthermore, electrochemical investigation of the modified glassy carbon revealed an excellent performance and a specific capacitance of 628.01 F g−1 at a 1 A g−1 current density. Moreover, an outstanding stability was demonstrated, with 89.9% retention of the capacitance observed after 3000 cycles at a 1 A g−1 current density.

Page 1 of 1 | Total Record : 10

 1 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol. 29, No. 3 Vol. 29, No. 2 Vol. 29, No. 1 Vol. 28, No. 4 Vol. 28, No. 3 Vol. 28, No. 2 Vol. 28, No. 1 Vol. 27, No. 4 Vol. 27, No. 3 Vol. 27, No. 2 Vol. 27, No. 1 Vol. 9, No. 1 Vol. 26, No. 4 Vol. 26, No. 3 Vol. 26, No. 2 Vol. 26, No. 1 Vol. 25, No. 4 Vol. 25, No. 3 Vol. 25, No. 2 Vol. 25, No. 1 Vol. 24, No. 4 Vol. 24, No. 3 Vol. 24, No. 2 Vol. 24, No. 1 Vol. 23, No. 4 Vol. 23, No. 3 Vol. 23, No. 2 Vol. 23, No. 1 Vol. 22, No. 4 Vol. 22, No. 3 Vol. 22, No. 2 Vol. 22, No. 1 Vol. 12, No. 1 Vol. 21, No. 4 Vol. 21, No. 3 Vol. 21, No. 2 Vol. 21, No. 1 Vol. 20, No. 4 Vol. 20, No. 3 Vol. 20, No. 2 Vol. 20, No. 1 Vol. 19, No. 4 Vol. 19, No. 3 Vol. 19, No. 2 Vol. 19, No. 1 Vol. 18, No. 4 Vol. 18, No. 3 Vol. 18, No. 2 Vol. 18, No. 1 Vol. 17, No. 3 Vol. 17, No. 2 Vol. 17, No. 1 Vol. 16, No. 3 Vol. 16, No. 2 Vol. 16, No. 1 Vol. 15, No. 2 Vol. 15, No. 1 Vol. 14, No. 2 Vol. 14, No. 1 Vol. 13, No. 2 Vol. 13, No. 1 Vol. 12, No. 2 Vol. 11, No. 2 Vol. 11, No. 1 Vol. 10, No. 2 Vol. 10, No. 1 Vol. 9, No. 2 Vol. 8, No. 3 Vol. 8, No. 2 Vol. 8, No. 1 Vol. 7, No. 3 Vol. 7, No. 2 Vol. 7, No. 1 Vol. 6, No. 3 Vol. 6, No. 2 Vol. 6, No. 1 More Issue