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Nonny Manampiring
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INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 78 Documents
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Isolation and Identification of Lactic Acid Bacteria from Red Dragon Fruit (Hylocereus polyrhicus) as Exopolysaccharide Producers Tatebale, Rivaldo; Naharia, Orbanus; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.7730

Abstract

Cherry tomatoes are a type of Red Dragon Fruit that has various benefits, including lowering cholesterol levels, preventing colon cancer, and strengthening the working power of muscles. Isolation of LAB isolated from Dragon Fruit as a production material for EPS. This study aims to isolate and identify LAB as a producer of EPS from red dragon fruit (Hylocereus polyrhiruz), which can produce exopolysaccharides. This research uses a descriptive research method. Data from experimental research in the laboratory obtained 10 LAB isolates, namely isolates NG1, NG2, NG3, NG4, NG5, NG6, NG7, NG8, NG9 and NG10. Based on the identification results, isolate NG1 has similarities with the genus Lactococcus (spherical, gram-positive, nonmotile, non-spore). While isolates NG2, NG3, NG4, NG5, NG6, NG7, NG8, NG9 and NG10 have similarities with the genus Lactobacillus (rod form, nonmotile, gram-positive). Based on the morphological characteristics, which are gram-positive, catalase-negative and non-spore. The ten isolates of lactic acid bacteria are capable of producing EPS. these are the results of EPS acquisition, namely: NG1(152.1 mg/L), NG2(127.9 mg/L), NG3(134.6 mg/L), NG4(130.9 mg/L), NG5(137 mg/L), NG6(139.2 mg/L), NG7(204.9 mg/L), NG8(156.2 mg/L), NG9(136.4 mg/L), and NG10 (157, 3 mg/L). The highest amount of EPS was isolated NG7 at 204.9 mg/L. Meanwhile, the lowest EPS was isolated NG4 at 130.9 mg/L.
Analysis of Clean Water Quality in Kolongan Village, Talawaan District Tarigan, Irama Rauli Tarigan; Rampengan, Mercy; Ogi, Nova L I M
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.7784

Abstract

Kolongan Village is one of the villages in Talawaan district of North Minahasa, North Sulawesi. In the village of Kolongan, some springs are potentially a clean water source for the people of the village. This research aims to analyze the quality of the springs in the village of Kolongan Talawaan, Prefectures as a water source. This type of research is quantitative, and the method used in this research is the Indonesian National Standard (SNI) according to the water quality parameters that have been established with the direct observation plan of the physical properties of water and with the laboratory testing plan for the observation of water chemical and biological properties with indicators of iron, sensitivity, manganese, E.coli, taste, and smell. The research was conducted from July 24, 2021, to September 20, 2021. The water quality measurement results in the spring in one village of Kolongan showed iron <0.0086 mg/L, iron 69-93 mg/ L, manganese 0.03-0.07 mg / L, E. coli, 11 CFU, not sensitive and odorless. Water source 2 showed iron <0.086 mg / l, density 75-85 mg/ l, manganese 0.02-0.03 mg/l, and E. coli, 3.6-9.2 CFU, is not sensible and odorless. The pollution index calculation indicates that the springs in Kolongan Village belong well. However, because they still contain biological reservoirs, it is necessary to have an advanced process for them to be consumed by the community.
Growth and Yield of Lettuce Plants (Lactuca sativa L.) with Alternative Nutrition to Subtitute AB Mix in Hydroponic Systems Toheni, Fredrik; Mege, Revolson; Satiman, Utari
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9075

Abstract

The hydroponic system is modern agricultural cultivation with various planting media as a substitute for soil, so hydroponics is an agricultural activity that is carried out by using water as a medium for distributing nutrients. This research aims to determine the effect of alternative nutrition to substitute AB mix on the growth and yield of hydroponic lettuce (Lactuca sativa L.). The research method was an experiment in a completely randomized design(CRD), using 5treatments with 4 repetitions, namely P0 (control/with ABmix nutrition) of 700-800 ppm, P1 (800-900 alternative nutrients), P2 (900-1000 ppm alternative nutrients), P3 (1000-1100 alternative nutrients), P4 (1000-1200 alternative nutrients), were analyzed by one way ANOVA analysis with a significance level of 0.05. Parameters observed were plant height, number of leaves, root length, leaf width, and fresh weight. The results showed that providing alternative nutrition significantly affected plant height, leaf width, and fresh weight of lettuce (Lactuca sativa L.). With the best treatment of 900-1000 ppm with plant height 35-41 cm, number of leaves 15-17, leaf width 17-18 cm, and plant wet weight 120-209 grams, the lowest yield was in treatment P0 with plant height 30-33 cm. the number of leaves is 15-16, and the wet weight of the plant is 117-146 grams
Analysis of Dominant Number Values Weed Population in Paddy Rice Cultivation (Oryza sativa, L.) in Koya Village, Minahasa, North Sulawesi Kasim, Yuriko; Lihiang, Anatje; Gedoan, Sukmarayu; Tanor, Meity; Yalindua, Aser; Sumampouw, Herry
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9076

Abstract

Paddy rice is the main food crop in Indonesia because rice is the primary food source of the Indonesian people. However, it faces obstacles caused, among others, by the presence of nuisance plants, namely weeds, that can reduce the quantity and quality of harvest or production. This study examines the value of the dominant Number of weed populations in rice field cultivation. The research method used was weed observation with analysis of the chief number value, using the square of a sample plot measuring 50 cm x 50 cm, randomly placed on the experimental land. The dominant number value analysis was carried out four times at the beginning of the study as an initial vegetation analysis, 21 DAP observations, 45 DAP observations, and 60 DAP observations. The results showed that the weed species with the highest Dominant Number Value at initial words in paddy fields were Paspalum disticum, Fibristylis litoralis, Marsilea crenata, Cyperus iria, and Ludwigia octovalvis; weed species with the highest Dominant Number Value at 21 DAP observations were P. disticum, Echinochloa crusgalli, F. litoralis, M. crenata, and Leersia hexandra; The weed species with the highest Dominant Number Value at 45 DAP observations were P. disticum, F. litoralis, C. iria, E. crusgalli, and Leersia hexandra and the weed species with the highest Dominant Number Values at 60 DAP observations were E. crusgalli, P. disticum, F. litoralis, Scirpus juncoides, and S. juncoides
Local Knowledge on Towards The Conservation of Macaca nigra in KPHK Tangkoko, North Sulawesi Djafar, Sintia; Rampengan, Mercy; Yalindua, Aser
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9159

Abstract

The Yaki monkey (Macaca nigra) is a critically endangered endemic species of North Sulawesi and is listed in Appendix II of CITES as well as in-situ conservation at the Tangkoko Conservation Forest Management Union (KPHK Tangkoko). The public's knowledge of the conservation of M. nigra is a key factor inining the sustainability of this species. The aim of this research is to find out the level of public awareness about conservation of M. nigra. This research uses descriptive methods. Data collection is done with a questionnaire with scored using the Guttman scale. The results of this study show that the majority of the level of knowledge in the community in the buffer zone KPHK Tangkoko belongs to a level of good, sufficient, and insufficient to the conservation status of m. nigra. Respondents who have good knowledge are all respondents in the Batu Putih Bawah villages and Duasudara villages. While the majority of the population in the Kasawari villages (63%) and the Pinangunian villages (68%) have sufficient knowledge about the conservation status of the M. nigra while almost half of the Kasawari villages (37%) and Pinangunian villages (32%) have insufficient knowledge.
The Antioxidant Activity of Red Fruit Extract (Pandanus conoideus L.) From Nabire Papua Migau, Natalina; Mokosuli, Yermia; Wurarah, Masje; Manampiring, Nonny
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9174

Abstract

Red fruit (Pandanus conoideus Lam) is a native Papuan plant which has been used by indigenous Papuan people as a medicinal plant. Papuan people believe that red fruit has a broad spectrum of pharmacological activity. This research aims to obtain antioxidant activity, namely reducing DPPH free radicals from Papuan red fruit ethanol extract. Samples of red fruit obtained from Nabire Papua. After determination, the red fruit is extracted using the maceration method. The ethanol extract of red fruit was then used for the DPPH antioxidant test method. The results showed that the ethanol extract of red fruit contains phenolic compounds: flavonoids, tannins; and steroids in high intensity. Ethanol extract of red fruit has very strong antioxidant activity (46.99 ppm; R2=0.99). Ethanol extract of Papuan red fruit has potential as a source of bioactive natural antioxidants.
THE EFFECT OF RICE HUSK CHARCOAL ON THE GROWTH AND PRODUCTION OF WHITE CHILI CAYEY (Capsicum frutescens L) Makalew, Evaristus; Naharia, Orbanus; Nanlohy, Fanny
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10751

Abstract

The aim of this research is: to determine and analyze the effect of giving rice husk charcoal on the growth and production of white cayenne pepper plants (Capsicum frutescens L). The method used in this research is the experimental method with a completely randomized design (RAL) approach. This shows that rice husk charcoal and planting media have different average values for plant height, number of leaves, number of branches, number of fruit and weight of white cayenne pepper. At the 12th week of observation, the M2ASP2 treatment had the highest value compared to other treatments, namely124.65 ± 31.73cm. Meanwhile treatment M1 ASP 0has the lowest value, namely79.23 ± 21.9cm.
APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

Abstract

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.
ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

Abstract

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category
TESTING FOR BACTERIAL CONTAMINATION ON CHICKEN (Gallus gallus domesticus) IN THE TRADITIONAL MARKET OF SOUTH LANGOWAN DISTRICT Sambeka, Delvino; Mege, Revolson; Setyawati, Iriani; Lawalata, Helen J; Rompas, Christny Ferdina Evie
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10754

Abstract

Chicken meat (Gallus gallus domesticus) plays an important role in society with its role as one of the sources of fulfillment of animal protein in Indonesia. With the high consumption of chicken meat by the public, the hygiene and safety of chicken meat must be ensured so as not to incur losses to the people. This study aimed to determine the bacterial contamination based on the testing of Total Plate Counts, Coliform, Escherichia coli, and Salmonella sp. Meet the requirement of the Indonesian National Standard on chicken meat in the traditional market of Langowan Selatan District. The method used in this research is a descriptive method to analyze and describe the results of the study, the testing using several indicators to directly conclude bacterial contamination. The results of the total plate count bacterial contamination test of the colonies, TPC in sample 1 = 29,1x kol/, sample 2 = 29,4x kol/, sample 3 = 27,7x kol/. MPN of Coliform and Escherichia coli results obtained in samples S1, S2, and S3 are 11x kol/gr. The results of microbact Salmonella sp. In samples S1 are positive, and S2 and S3 are negative. Based on observations, it is suspected that contamination occurs because sellers use equipment that is less sterile and there is no cover or barrier so the chicken meat is in direct contact with the air and customer. It is concluded that based on bacterial testing indicators, chicken meat in the traditional market of Langowan Selatan District has exceeded the limit of SNI 7388 2009 and is contaminated.

Page 7 of 8 | Total Record : 78

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