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Nonny Manampiring
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Jl, Kampus Unima, Tonsaru, Minahasa - North Sulawesi. Indonesia 95632
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INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 78 Documents
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CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Abstract

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.
IDENTIFICATION OF ESCHERICHIA COLI AND SALMONELLA SP. ON FRIED SNACKS SOLD ON THE UNIMA CAMPUS ENVIRONMENT Runtunuwu, Yudistira Deyvan; Lawalata, Helen J; Tengker, Anita
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10756

Abstract

This study aims to explain how bacterial contamination and the presence of Escherichia coli and Salmonella sp. on fried bananas sold in the UNIMA campus environment. This exploration used clear qualitative subjective observation with the TPC strategy for all total bacterial contamination and involved specific media for Escherichia coli and Salmonella sp. From the results of the review, it was found that 1 out of 3 experimental examples of complete bacterial contamination exceeded the threshold set by the Food and Drug Supervisory Agency of the Republic of Indonesia (BPOM RI) in 2016, with the most extreme obstacle being the number of microorganisms in food being 10-4 colonies/ g. Of the 3 samples tried, all samples were identified with Escherichia coli and Salmonella sp. Based on the research conducted, it can be concluded that, of all the observed fried samples, all detected bacterial colonies
Analysis of Weed Vegetation on Paddy Rice (Oryza sativa) Plant in Koya Village, South Tondano District Lazar, Noviana; Naharia, Orbanus; Taulu, Marthy
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10759

Abstract

Paddy Rice (Oryza sativa) is the main food commodity in Indonesia, because most of the Indonesian population's staple food is Paddy Rice. Demand for Paddy Rice continues to increase from time to time along with population growth. The aim of this research is to determine the types and control of weeds in lowland Paddy Rice plants (Oryza sativa). This research is a qualitative descriptive study using a survey method directly observing weeds in the Paddy Rice planting area in Koya village, South Tondano subdistrict. The results of research on the density, dominance, and frequency of weed types in lowland Paddy Rice plants in Koya Village, South Tondano District show that there are 4 types of weeds in lowland Paddy Rice plants in Koya Village, namely Panicum repens, Fimbristylis litotoralis, Cyperus rotundus, and Pistia stratiotes. P.repens is a grass weed, F.litoralis and C.rotundus are a weed, and P.stratiotes is a broadleaf weed. The type that has the highest relative density (KR) is P. repens, namely 3.33%, followed by F. litoratlis, namely 26.67%, and the lowest is C. rotundus and P. stratiotes, namely 20.0%. Relative Dominance Value The highest (DR) was found in the weed type P. stratiotes, namely 51.56%, then followed by C. rotundus, namely 19.27%, F. litoralis, namely 15.10%, and the lowest, P. repens, namely 14.06%. The highest relative frequency (FR) was in P. repens and C. rotundus, namely 33.33%, followed by F. litoralis, namely 22.22% and the lowest was P. stratiotes, namely 11.11%. Based on the research results, it can be concluded that there are 4 types of weeds in lowland Paddy Rice plants in Koya Village, South Tondano District with the highest weed dominance index value, namely P. stratiotes, namely 27.55787; Weed control can be done chemically, biologically, mechanically and physically.
Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus Pua, Devis Christian; Mokosuli, Yermia Semuel; Sumampouw, Harry Maurits
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10760

Abstract

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.
IDENTIFICATION OF MACROALGAE IN THE COASTAL AREA OF BUDO TOURISM VILLAGE, WORI, NORTH MINAHASA Watung, Fernando; Supit, Annely; Setyawati, Iriani; Kamagi, Decky; Agustina, Tika Puri; Rukmana, Musma; Hasmiati, Hasmiati
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10837

Abstract

Algae are lower plants that do not have differentiated structures such as roots, stems, and leaves; although they may appear to have differences, they are actually just thallus forms. Macroalgae have macroscopic body shape and size consisting of fronds without distinct roots, stems, and leaves. Macroalgal diversity is not only reflected in their ecological and economic significance but also in their high species richness and wide geographical distribution within aquatic ecosystems. However, research on the species diversity of macroalgae in Budo Tourism Village Beach, North Minahasa remains limited. This study aims to identify the macroalgal species in the Budo Tourism Village, Wori, North Minahasa area. The research employed the line transect and quadrat-based sampling method, which was implemented at two distinct station locations: Station I (rocky reef substrate) and Station II (sandy-muddy substrate). Macroalgae identification was facilitated by utilizing the comprehensive identification textbooks. The research reveals the presence of four macroalgal species, comprising three brown algae (Phaeophyceae), namely Padina boergesenii, Sargassum llinearifolium, Turbinaria ornata and one red algae (Rhodophyceae), Gracilaria corticata. Sargassum linearifolium, Padina boergesenii, and Gracilaria corticata was found in rocky reef substrate. Turbinaria ornate and Padina boergesenii was found in sandy-muddy substrate.
GROWTH ACTIVITY TEST OF WHITE RAT HAIR COMBINATION OF CELERY (Apium Graveolens) LEAF EXTRACT AND GREEN GEDI (Abelmoschus Manihot) LEAF Kawatu, Chelsea; Lebang, Julianri Sari; Jayanti, Meilani
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.11058

Abstract

Celery and Green Gedi leaves have been long considered traditional topical supplements to support hair growth. They contain flavanoids that are efficacious to induce keratinocyte proliferation. However, the research about whether the combination of celery and green gedi leaves can still provide an effect is limited. This current study aims to determine the effect of the combination of ethanol extracts of celery leaves and green gedi leaves on white rat hair growth. This is laboratory experimental research to compare the hair growth in three different groups with normal and positive control. Animals were randomly grouped (n=3 for each group) and then given a combination of leaf extracts and carbopol with a concentration of 17.5%:10%; 27.5%:15%;37.5%:20%. A normal control group was left untreated, while another group was treated with 2% minoxidil as positive control. The results of measuring fur weight on day 21 showed that the treatment group given the combination of extracts did affect the growth of rat hair. Statistical tests using one-way ANOVA showed a p-value of <0.001 which means there is a significant difference in the average hair weight of rats treated. LSD post hoc test showed that treatment with a 17.5%:10% concentration ratio was significantly different from the other treatments. It can be concluded that the combination of celery leaf extract and gedi leaf positively affects rat hair growth.
ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Abstract

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.
Anticancer Activity Of Apis Dorsata Binghami Nest Extract From West Uluway, Tana Toraja District Patandean, Selvrining; Mokosuli, Yermia Semuel; Ogi, Nova LIM; Djarang, Rievo H; Manampiring, Nonny; Moko, Emma Mauren
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12750

Abstract

Cancer is the leading cause of death in the world, with a high prevalence in Indonesia, especially breast cancer. Conventional therapies such as chemotherapy and radiotherapy have various limitations, including severe side effects and cancer cell resistance. This study aims to explore the anticancer potential of the ethanol extract of Apis dorsata Binghami nests originating from Uluway Barat Village, Tana Toraja Regency. Extraction was carried out using the maceration method with 96% ethanol solvent, with a yield of 51.42%, and bioactive compounds were identified through GC-MS analysis. Anticancer activity tests were carried out in vitro on MCF-7 breast cancer cells using the resazurin assay method . The results showed that the extract contained various bioactive compounds such as flavonoids, furanones, and aromatic aldehydes which are known to have antioxidant and anticancer activities. The IC50 value obtained was 434.30 µg/mL, indicating weak cytotoxic activity. Nevertheless, this extract showed significant ability to kill cancer cells at certain concentrations, especially 15.63 µg/mL which reduced cell viability by 44.39%. These results indicate that A. dorsata Binghami nests contain compounds that have the potential as natural anticancer agents, but require further research for fractionation and identification of more specific active compounds and further testing in vivo

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