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Nonny Manampiring
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INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 87 Documents
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APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

Abstract

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.
ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

Abstract

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category
TESTING FOR BACTERIAL CONTAMINATION ON CHICKEN (Gallus gallus domesticus) IN THE TRADITIONAL MARKET OF SOUTH LANGOWAN DISTRICT Sambeka, Delvino; Mege, Revolson; Setyawati, Iriani; Lawalata, Helen J; Rompas, Christny Ferdina Evie
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10754

Abstract

Chicken meat (Gallus gallus domesticus) plays an important role in society with its role as one of the sources of fulfillment of animal protein in Indonesia. With the high consumption of chicken meat by the public, the hygiene and safety of chicken meat must be ensured so as not to incur losses to the people. This study aimed to determine the bacterial contamination based on the testing of Total Plate Counts, Coliform, Escherichia coli, and Salmonella sp. Meet the requirement of the Indonesian National Standard on chicken meat in the traditional market of Langowan Selatan District. The method used in this research is a descriptive method to analyze and describe the results of the study, the testing using several indicators to directly conclude bacterial contamination. The results of the total plate count bacterial contamination test of the colonies, TPC in sample 1 = 29,1x kol/, sample 2 = 29,4x kol/, sample 3 = 27,7x kol/. MPN of Coliform and Escherichia coli results obtained in samples S1, S2, and S3 are 11x kol/gr. The results of microbact Salmonella sp. In samples S1 are positive, and S2 and S3 are negative. Based on observations, it is suspected that contamination occurs because sellers use equipment that is less sterile and there is no cover or barrier so the chicken meat is in direct contact with the air and customer. It is concluded that based on bacterial testing indicators, chicken meat in the traditional market of Langowan Selatan District has exceeded the limit of SNI 7388 2009 and is contaminated.
CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Abstract

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.
IDENTIFICATION OF ESCHERICHIA COLI AND SALMONELLA SP. ON FRIED SNACKS SOLD ON THE UNIMA CAMPUS ENVIRONMENT Runtunuwu, Yudistira Deyvan; Lawalata, Helen J; Tengker, Anita
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10756

Abstract

This study aims to explain how bacterial contamination and the presence of Escherichia coli and Salmonella sp. on fried bananas sold in the UNIMA campus environment. This exploration used clear qualitative subjective observation with the TPC strategy for all total bacterial contamination and involved specific media for Escherichia coli and Salmonella sp. From the results of the review, it was found that 1 out of 3 experimental examples of complete bacterial contamination exceeded the threshold set by the Food and Drug Supervisory Agency of the Republic of Indonesia (BPOM RI) in 2016, with the most extreme obstacle being the number of microorganisms in food being 10-4 colonies/ g. Of the 3 samples tried, all samples were identified with Escherichia coli and Salmonella sp. Based on the research conducted, it can be concluded that, of all the observed fried samples, all detected bacterial colonies
Analysis of Weed Vegetation on Paddy Rice (Oryza sativa) Plant in Koya Village, South Tondano District Lazar, Noviana; Naharia, Orbanus; Taulu, Marthy
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10759

Abstract

Paddy Rice (Oryza sativa) is the main food commodity in Indonesia, because most of the Indonesian population's staple food is Paddy Rice. Demand for Paddy Rice continues to increase from time to time along with population growth. The aim of this research is to determine the types and control of weeds in lowland Paddy Rice plants (Oryza sativa). This research is a qualitative descriptive study using a survey method directly observing weeds in the Paddy Rice planting area in Koya village, South Tondano subdistrict. The results of research on the density, dominance, and frequency of weed types in lowland Paddy Rice plants in Koya Village, South Tondano District show that there are 4 types of weeds in lowland Paddy Rice plants in Koya Village, namely Panicum repens, Fimbristylis litotoralis, Cyperus rotundus, and Pistia stratiotes. P.repens is a grass weed, F.litoralis and C.rotundus are a weed, and P.stratiotes is a broadleaf weed. The type that has the highest relative density (KR) is P. repens, namely 3.33%, followed by F. litoratlis, namely 26.67%, and the lowest is C. rotundus and P. stratiotes, namely 20.0%. Relative Dominance Value The highest (DR) was found in the weed type P. stratiotes, namely 51.56%, then followed by C. rotundus, namely 19.27%, F. litoralis, namely 15.10%, and the lowest, P. repens, namely 14.06%. The highest relative frequency (FR) was in P. repens and C. rotundus, namely 33.33%, followed by F. litoralis, namely 22.22% and the lowest was P. stratiotes, namely 11.11%. Based on the research results, it can be concluded that there are 4 types of weeds in lowland Paddy Rice plants in Koya Village, South Tondano District with the highest weed dominance index value, namely P. stratiotes, namely 27.55787; Weed control can be done chemically, biologically, mechanically and physically.
Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus Pua, Devis Christian; Mokosuli, Yermia Semuel; Sumampouw, Harry Maurits
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10760

Abstract

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.
IDENTIFICATION OF MACROALGAE IN THE COASTAL AREA OF BUDO TOURISM VILLAGE, WORI, NORTH MINAHASA Watung, Fernando; Supit, Annely; Setyawati, Iriani; Kamagi, Decky; Agustina, Tika Puri; Rukmana, Musma; Hasmiati, Hasmiati
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10837

Abstract

Algae are lower plants that do not have differentiated structures such as roots, stems, and leaves; although they may appear to have differences, they are actually just thallus forms. Macroalgae have macroscopic body shape and size consisting of fronds without distinct roots, stems, and leaves. Macroalgal diversity is not only reflected in their ecological and economic significance but also in their high species richness and wide geographical distribution within aquatic ecosystems. However, research on the species diversity of macroalgae in Budo Tourism Village Beach, North Minahasa remains limited. This study aims to identify the macroalgal species in the Budo Tourism Village, Wori, North Minahasa area. The research employed the line transect and quadrat-based sampling method, which was implemented at two distinct station locations: Station I (rocky reef substrate) and Station II (sandy-muddy substrate). Macroalgae identification was facilitated by utilizing the comprehensive identification textbooks. The research reveals the presence of four macroalgal species, comprising three brown algae (Phaeophyceae), namely Padina boergesenii, Sargassum llinearifolium, Turbinaria ornata and one red algae (Rhodophyceae), Gracilaria corticata. Sargassum linearifolium, Padina boergesenii, and Gracilaria corticata was found in rocky reef substrate. Turbinaria ornate and Padina boergesenii was found in sandy-muddy substrate.
ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Abstract

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.
Analysis Of The Potential Of Natural Sterol Compounds From Tin (Ficus carica) Leaves as Anti-Hypercholesterolemia with In Silico Tests Taihuttu, Monalisa Pertiwi Jeriska; Manurung, Brian Saputra; Simal, Rufiati; Watung, Fernando A.
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.11961

Abstract

This study aims to determine the potential of natural sterol compounds from Tin (Ficus carica) leaves as anticholesterolemic using in silico test. This study employed an in silico approach using molecular docking methods to evaluate the potential of sterol compounds as anti-hypercholesterolemic agents, in comparison to other references compounds. Data processing and interpretation were conducted using molecular databases for both ligands and target proteins. The analysis utilized several computational tools, including PyRx 0.8, PyMOL, LigPlus, and Discovery Studio 2016 Client. The compounds used in this research  were sterol, fluvastatin and simvastatin (as control), with HMG CoA reductase as the target protein. The results showed that the highest binding affinity value was fluvastatin which is -8.3 kcal/mol. Sterol compounds are compounds with lower binding affinity which is -7.7 kcal/mol. From the visualization results, it is known that the binding distance between sterol compounds with target proteins (HMG CoA reductase) is between 2.69 to 5.49 Å, and the binding distance between simvastatin compounds with target proteins (HMG CoA reductase) is between 2.76 to 5.36 Å, and the binding distance between fluvastatin compounds with target proteins (HMG CoA reductase) is between 1.95 to 5.26 Armstrong. While based on the comparison of the binding side of strerol, simvastatin and fluvastatin, it is known that the three compounds have the same site because they bind to the same amino acid residues, namely ARG (B: 515), TYR (A: 533), TYR (B: 533), TYR (B: 517), PRO (A: 511). The results of this research indicate that the natural sterol compounds found in Tin (Ficus carica) leaves have potential as anti-hypercholesterolemic agents, based on reverse docking analysis. These sterol compounds bind to the same active site as the control drugs, simvastatin and fluvastatin, and exhibit comparable binding affinity values

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