cover
Contact Name
Adi Ratriyanto
Contact Email
ratriyanto@staff.uns.ac.id
Phone
+628125917414
Journal Mail Official
jurnal.biotek.biodiv@mail.uns.ac.id
Editorial Address
Universitas Sebelas Maret
Location
Kota surakarta,
Jawa tengah
INDONESIA
Journal of Biodiversity and Biotechnology
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Articles 60 Documents
Effect of Concentration and Time of Chitosan Application on The Growth of Pineapple (Ananas comosus) Endang Yuniastuti; Salsabila Tiara Putri; Gani Cahyo Handoyo
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108238

Abstract

Pineapple (Ananas comosus) is a popular fruit crop in Indonesia. Pineapple crowns are one of the planting materials used in pineapple cultivation, but their growth is slower than other planting materials, so that the addition of chitosan is needed, which has the potential to stimulate growth. This study aims to determine the best concentration and application time of chitosan that can improve pineapple growth. The study was conducted in the screenhouse of the Faculty of Agriculture, Sebelas Maret University, Surakarta during March-June 2025 with a Completely Randomized Design (CRD) with two factors, namely chitosan concentration (without chitosan, 1 ppm, 3 ppm, 5 ppm) and time of chitosan application (morning, afternoon, morning and afternoon). Each treatment was repeated 4 times, resulting in 48 experimental units. The results showed that no combination of concentration and time of chitosan application was found to simultaneously increase pineapple growth. The treatment concentration of 1 ppm and 3 ppm gave the best result to increasing leaf number; concentration 1 ppm, 3 ppm, and 5 ppm gave the best result to increasing total leaf area and longest root length. Time of chitosan application once in the morning or evening gave the best result to increasing photosynthesis rate and stomatal conductance. Chitosan has the potential as a biostimulant for plant growth and is effective in increasing the physiological activity of plants.
Yield Performance of Short-Stem Promising Mutant Lines of Mentik Wangi Rice Induced by 200 Gy Gamma Irradiation Fitria Roviqowati; Desfinenda Calistarajni; Parjanto Parjanto; Edi Purwanto; Ahmad Yunus
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.106011

Abstract

The demand for rice in Indonesia continues to increase but production has decreased. Efforts that can be made are the use of local varieties of mentik wangi rice. Mentik wangi rice has the disadvantages of long life, high stems, and low crop production. As a solution, plant breeding is carried out in the form of mutation by giving gamma irradiation which can improve unwanted genetic traits and produce superior varieties with the dose used of 200 gray. This study aims to evaluate the yield potential of promising mutant strains with 200 gray gamma irradiation on mentik wangi rice and get mutant strains that have superior criteria for short-stemmed, short-lived, and high-yielding. This research was conducted in the rice field of Ngampel Hamlet, Gentungan Village, Mojogedang, Karanganyar, Central Java. The research was conducted in June-October 2023. The design used was a Randomized Complete Group Design (RCBD) with a single factor, namely strains consisting of 14 mutant strains and 1 control strain with 3 repetitions. The results of observations were analyzed by Analysis of Variance (ANOVA) at the 5% level and continued with Duncan Multiple Range Test (DMRT) at the 5% level. All tested strains produced the expected superior criteria, namely short stem, short age, and high productivity.
Genetic Variability and Trait Correlations of Melon (Cucumis melo L.) Genotypes Heru Anggara; Dicky Marsadi; Listihani Listihani
Journal of Biodiversity and Biotechnology Vol 5, No 2 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i2.113228

Abstract

Melon (Cucumis melo L.) is an important horticultural crop. Melon breeding programs require comprehensive information on genetic variability and selection-related traits. Genetic variability and correlations among plant characters are essential parameters for effective selection in the S2 Population. This study aimed to evaluate the genetic variability of several melon genotypes and to analyze correlations among plant characters as a basis for selection. The experiment was conducted using fourteen melon genotypes derived from selfed populations at the S₂ generation. The study employed a randomized complete block design with a single factor, consisting of 14 S₂ melon genotypes and four replications. The experimental unit is one individual plant in a polybag. The Observations of qualitative traits indicated the presence of variation within the genotypes for fruit rind color and fruit flesh color. Genotypes G2 exhibited the greatest fruit length (13.47 cm) and fruit diameter (11.95 cm). Genotypes G15 showed the highest total soluble solids content (150 Brix), while genotypes G7 had the thickest fruit flesh (3.25 cm). Broad-sense heritability estimates of the observed traits ranged from low to moderate. The traits of fruit length, fruit diameter, and fruit weight exhibited moderate broad-sense heritability (>45%). They were positively and significantly correlated with fruit weight, indicating that these traits can be used as selection criteria for fruit weight in subsequent plant breeding programs.
DNA Damage in Prader Willi Syndrome Against Obesity Diennisa Mursalin
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.77619

Abstract

Prader Willi Syndrome is a multisystem genetic disorder caused by deletions of paternal chromosomes (75%), maternal chromosomal dysomi (25%), and translocations (1%). This syndrome has a main characteristic, namely the state of obesity in individuals sufferers where. This is due to deficiency of Growth Hormone levels due to central hypothalamic-limbic disorders that occur in this syndrome. This syndrome has a characteristic course of obesity in every phase of life. The obesity that is characteristic of this syndrome causes diverse complications because it causes DNA damage through chronic inflammation caused by an increase in pro-inflammatory cytokines due to the buildup of adipose tissue. Thus, individuals with PWS can experience various metabolic diseases that can increase their morbidity and mortality.
Isolation of Lactic Acid Bacteria from Soil and Its Antifungal Activity Against Aspergillus flavus Athiyyah Jihan Tifani; Tjahjadi Purwoko; Ari Susilowati
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.109898

Abstract

Food stocks, such as grains, fruits, and vegetables are vulnerable to spoilage by Aspergillus flavus, which typically controlled using chemical preservatives. Concerns about the health impact of these chemicals highlight the need for safer alternatives, such as biopreservatives. Lactic acid bacteria (LAB) are Generally Recognized as Safe (GRAS) microorganisms and have potential application as biopreservative agent, because LAB produced various metabolites with antifungal activities. LAB could be found in nutrient-rich environments, including rhizosphere soil and poultry house soil due to the nutrient residues for growth. The aims of this study were to isolate LAB from soils and to evaluate their antifungal activity with a focus on potential applications as biopreservatives. The LAB isolates from soil were characterized and screened for antifungal activity using the dual culture method. The LAB isolates inhibited A. flavus growth with the largest growth inhibition was showed by LAB isolate 1 that isolated from poultry house soil (17.65%). However, statistics analysis showed that fungal growth inhibition by LAB isolates were not significantly difference (p>0,05). Although the result was not statistically significant, all  LAB isolates significantly inhibited conidia development. This showed potential application of LAB isolates as biopreservative agent. Keywords: antifungal, Aspergillus flavus, biopreservative, food stock, lactic acid bacteria
APPLICATION OF BENZYL ADENINE AND IAA ON THE GROWTH OF BANANA BARANGAN SUBCULTURE (Musa acuminata L.) Andriyana Setyawati; Samanhudi Samanhudi; Edi Purwanto; Retna Bandriyati Arniputri; Kevin Putra Setyawan; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108497

Abstract

Barangan banana is a banana variety that has high economic value, but its production is still carried out conventionally, resulting in limited barangan bananas on the market. The use of tissue culture methods can quickly produce high-quality, disease-free plants regardless of seasonal conditions. The aim of this research is to determine the effect of providing the best ZPT IAA and BA for the growth of the Barangan banana planet. The research was conducted in August-October 2023 at the Biotechnology and Plant Physiology Laboratory, Sebelas Maret University. This research used a two-factor Completely Randomized Design (CRD) with 6 replications. The first factor was the use of ZPT Indole Acetic Acid (concentrations 0, 0.5, 1, and 1.5 ppm and the second factor was the use of ZPT Benzyl Adenine (0, 0.5, 1, 1.5 ppm). The variables observed were root emergence time, number of roots, root length, time of leaf emergence, number of leaves, time of shoot emergence, number of shoots, and height of shoots. The observed data were analyzed using Analysis of Variance (ANOVA) with a 5% level test and if there was a significant difference, it was continued with Duncan's Multiple Range Test (DMRT) at the level 5%. The results showed that giving 0.5 ppm IAA increased root length; 0.5 ppm IAA was given optimally for root formation; and 1 ppm IAA increased the shoot height of the Barangan banana plnalet. Giving BA 0.5 ppm root number; BA 1, 5 ppm produces the highest number of leaves; BA 1 ppm increases shoot height of barangan banana plantlets.
A review on the updated checklists of the amphibians and reptiles of Pakistan: Present and prehistoric Amtyaz Safi; Hans-Volker Karl
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.106964

Abstract

Recent herpetological collections have added several new taxa to the previously compiled herpetofauna of Pakistan. This paper presents annotated and updated checklists of 243 species, 104 genera, and 27 families of extant herpetofauna, including 24 anuran amphibian species, belonging to 4 families. In comparison, the reptilian fauna consists of 219 species/subspecies, including 2 species of crocodiles, 15 species of turtles and tortoises, 119 species of Sauria, and 83 species of snakes belonging to 23 families. Four species of amphibians and 33 species of reptiles are endemic to Pakistan. Separate checklists of prehistoric reptiles (50 taxa) for both dinosaurs (26 taxa) and non-dinosaurian taxa (24 taxa) are also presented here. This review article is based on previous literature on the herpetofauna of Pakistan. It will serve as a baseline for future work related to the herpetofauna of Pakistan, which is essential in biodiversity research.
The Effect of Various Fertilizer Doses on Sugarcane Seed Growth and Productivity Dony Agung Wicaksono; Deny Bagus Surendra; Febry Ari Marpaung; Nanik Tri Ismadi
Journal of Biodiversity and Biotechnology Vol 5, No 2 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i2.111756

Abstract

Fertilization plays a crucial role in promoting plant growth, increasing yield, and improving crop quality. Current fertilization recommendations for sugarcane are generally based on production-oriented cultivation with a 12-month growth period, while specific fertilization requirements for seed sugarcane remain insufficiently studied. This study aimed to determine the optimal fertilizer dosage for sugarcane seed by evaluating the effects of different fertilizer combinations on plant growth and productivity. The research was conducted at the Experimental Farm of Puslit Sukosari. The experimental area covered 0.046 ha, with planting rows 8 m in length and a row factor of 1042 rows/ha. Four fertilizer treatments were applied: (A) ZA 640 kg/ha, SP 36 48.1 kg/ha, and KCl 255 kg/ha; (B) ZA 531 kg/ha, SP 36 40 kg/ha, and KCl 213 kg/ha; (C) ZA 427.5 kg/ha, SP 36 31.9 kg/ha, and KCl 171 kg/ha; and (D) ZA 321.3 kg/ha, SP 36 23.9 kg/ha, and KCl 129 kg/ha. The results showed that increasing fertilizer dosage did not result in a linear increase in seed sugarcane productivity. The highest seed productivity and optimal growth performance, including the number of stalks, stalk height, stalk diameter, and seed weight per hectare, were obtained under Treatment B. In contrast, the lowest productivity was observed under Treatment D. Considering both fertilization efficiency and production costs during the vegetative growth phase, seed sugarcane plantations may be fertilized using the complete fertilizer combination in Treatment B or solely with nitrogen fertilizer at a rate of ZA 531 kg/ha.
Application of ZA Fertilizer on Growth and Production of Red Ginger (Zingiber officinale var. Rubrum) Albriansyah Zinka Ancoro Putro; Supriyono Supriyono; Sulandjari Sulandjari; Djoko Purnomo
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.74442

Abstract

Red ginger is one of the export commodities of spices in Indonesia which plays a significant role in the country's foreign exchange earnings. In order to meet market demand, ginger cultivation needs to be fertilized both organically and inorganically to increase production. ZA fertilizer for plants serves to stimulate vegetative growth. This study aims to determine the response of red ginger to the application of ZA fertilizer has a significant effect on the growth and yield of red ginger and to obtain the optimum dose of ZA fertilizer for the growth and yield of red ginger. This research was carried out in March - August 2021 until it was completed in Pelem Hamlet, Wonorejo Village, Jatiyoso District, Karanganyar Regency, Central Java Province with coordinates 7°43'24.7”S 111°05'31.2”E. The experimental design was a Completely Randomized Complete Block Design (RCBD). This study consisted of 4 treatments with 6 replications so that 24 experimental units were obtained, while the treatments in this study were as follows: P0: Control without ZA fertilizer, P1: Treatment 225 Kg ZA/ha or 22.5 g/m2, P2: Treatment 450 Kg ZA/ha or 45 g/m2, and P3: Treatment 675 Kg ZA/ha or 67.5 g/m2. The results showed that ZA fertilizer had no significant effect on the yield of red ginger, but on growth observations, P2 treatment with a dose of 450 Kg ZA/ha can have a significant effect on the increase in the number of plants per plot. The dry weight of the stover was strongly correlated with the weight of fresh rhizomes with correlation values (r) 0.508.
Comparative Analysis of Bioacoustic and Point Count Methods for Calculating Bird Species Richness in Bukit Watu Ondo, Mount Ungaran, Central Java Divanda Lathiifanindya; Vika Widyaningrum; Yudistira Nurpratama; Maesa Faradina; Ni’mah Alawiyah Safitri; Margareta Rahayuningsih
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.110928

Abstract

The study aims to compare the effectiveness of bioacoustic and point count methods in assessing bird species richness in Bukit Watu Ondo, Mount Ungaran, Central Java. A quantitative approach was employed through simultaneous data collection using both methods at six observation points. Bird observations were conducted visually and acoustically for 15-minute sessions at each point, repeated three times. The data were analyzed using a paired t-test to determine statistical differences between the two methods. Results showed that the point count method identified 29 species from 22 families, while the bioacoustic method recorded 25 species from 17 families. Statistical analysis revealed no significant difference (p = 0.927) between the two methods in the number of detected species, indicating comparable effectiveness. However, bioacoustics proved more efficient in detecting vocally active or cryptic species that were difficult to observe visually, whereas point count performed better for visually conspicuous species. The combination of both methods provided a more comprehensive representation of avifaunal diversity. These findings demonstrate that bioacoustic techniques are a viable and complementary alternative to traditional visual methods in biodiversity monitoring and conservation management.