Journal of Biodiversity and Biotechnology
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Articles
60 Documents
<![CDATA[Effects of Rice Varieties on Attractiveness, Population Development, and Weight Loss Caused by Sitophilus oryzae During Storage ]]>
<![CDATA[Dicky Marsadi]]>;
<![CDATA[Ketut Ayu Yuliadhi]]>
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 5, No 2 (2025) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v5i2.113966
<![CDATA[Sitophilus oryzae is one of the major pests of stored rice, causing both quantitative and qualitative losses during storage. This study aimed to analyze the effects of different rice varieties on attractiveness, population development, and weight loss resulting from S. oryzae infestation. The research was conducted using a free-choice attractiveness test, population development observations, and weight loss measurements on four rice varieties, namely red rice, white rice, black rice, and brown rice. The results indicated that rice variety significantly affected the behavior and population development of S. oryzae. Red rice exhibited the highest level of attractiveness and the greatest population growth rate, followed by black rice and brown rice, whereas white rice showed the lowest attractiveness, population growth rate, and weight loss. The pattern of rice weight loss corresponded to pest population levels, with varieties harboring higher S. oryzae populations experiencing greater percentages of weight loss. Differences in S. oryzae responses among rice varieties are likely associated with variations in the physical and chemical characteristics of the grains, particularly nutrient content and grain layer structure, which influence feeding preference and developmental success of the pest. These findings suggest that rice varieties have potential as an important component of postharvest pest management strategies, especially in reducing storage losses through the selection of varieties that are relatively more resistant to S. oryzae infestation.]]>
<![CDATA[Novelty, Distinctiveness, Uniformity, and Stability Testing for Three Potential Lines of Black Rice ]]>
<![CDATA[Nandariyah - Nandariyah]]>;
<![CDATA[Yuyun Nitasari]]>;
<![CDATA[Bambang Pujiasmanto]]>;
<![CDATA[Endang Yuniastuti]]>
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v3i2.80676
<![CDATA[Black rice Cempo Ireng is a local variety with a long plant life, high plant posture, and low productivity. Plant breeding by gamma irradiation was carried out to correct these weaknesses. The results of the gamma irradiation process included a selection process for each generation and produced potential lines numbers such as 8, 51, and 52. The characterization was based on the NDUS (Novelty, Distinctiveness, Uniformity, and Stability) test. The purpose of this study was to characterize potential lines as one of the requirements for the release of plant varieties. The study was conducted in the paddy fields of Pakahan Village, Jogonalan District, Klaten Regency, from February-July 2022. The method used was a single factor randomized complete block design with three replications. Data were analyzed by T test with 5% level. The results of the study showed that the three potential lines have differences with the parent and the Sembada varieties. Three potential lines can be deemed to be new, dictinct, uniform, and stable.]]>
<![CDATA[The Effect of Naphtaleine Acetic Acid ND Benzyl Amino Purine on the Subculture of Kepok Unti Sayang Banana ]]>
<![CDATA[Samanhudi Samanhudi]]>;
<![CDATA[Andriyana Setyawati]]>;
<![CDATA[Edi Purwanto]]>;
<![CDATA[Muji Rahayu]]>;
<![CDATA[Eviani Eviani]]>;
<![CDATA[Muhammad Aji Cahyadi]]>
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v5i1.108513
<![CDATA[The Kepok Unti Sayang variety is a superior type of banana that is resistant to fusarium wilt. The high demand for Kepok Unti Sayang needs to be balanced with good seed production. One of the methods used for multiplying superior seeds of Kepok Unti Sayang is through tissue culture. This research aims to obtain the optimal concentration of Naphtaleine Acetic Acid (NAA) in the subculture media for the growth of root numbers in the Kepok Unti Sayang Banana. The second objective is to determine the optimal concentration of Benzyl Amino Purine (BAP) in the subculture medium for shoot number growth in Kepok Unti Sayang bananas. Another goal is to identify the best treatment combination that can produce the highest number of shoots in Kepok Unti Sayang bananas. The research was conducted at the Laboratory of Plant Physiology and Biotechnology at Sebelas Maret University from February to July 2025. The study was conducted with 4 levels of NAA treatment: 0; 0.5; 1; and 1.5 ppm. The BAP treatment was administered at 4 levels: 0; 1; 2; and 3 ppm. The variables observed in this study were the time of shoot emergence, number of shoots, time of root emergence, root length, and final height of the planlet. Data from each variable were then analyzed using analysis of variance (ANOVA) with an F-test at a significance level of 95%. Significantly different data were then followed by Duncan's Multiple Range Test (DMRT) at a significance level of 95%. The significant combination of NAA and BAP on the observed variables was then reanalyzed using regression tests. The results showed that the application of various concentrations of NAA does not affect the growth of the number of roots of Kepok bananas var. Unti Sayang. The application of 2 ppm BAP is optimal for the growth of the number of shoots of Kepok bananas var. Unti Sayang. The interaction between 1 ppm NAA and BAP at 1-2 ppm produces the most optimal average shoot emergence time.]]>
<![CDATA[Effect of Concentration and Time of Chitosan Application on The Growth of Pineapple (Ananas comosus) ]]>
<![CDATA[Endang Yuniastuti]]>; Salsabila Tiara Putri; Gani Cahyo Handoyo
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v4i2.108238
<![CDATA[Pineapple (Ananas comosus) is a popular fruit crop in Indonesia. Pineapple crowns are one of the planting materials used in pineapple cultivation, but their growth is slower than other planting materials, so that the addition of chitosan is needed, which has the potential to stimulate growth. This study aims to determine the best concentration and application time of chitosan that can improve pineapple growth. The study was conducted in the screenhouse of the Faculty of Agriculture, Sebelas Maret University, Surakarta during March-June 2025 with a Completely Randomized Design (CRD) with two factors, namely chitosan concentration (without chitosan, 1 ppm, 3 ppm, 5 ppm) and time of chitosan application (morning, afternoon, morning and afternoon). Each treatment was repeated 4 times, resulting in 48 experimental units. The results showed that no combination of concentration and time of chitosan application was found to simultaneously increase pineapple growth. The treatment concentration of 1 ppm and 3 ppm gave the best result to increasing leaf number; concentration 1 ppm, 3 ppm, and 5 ppm gave the best result to increasing total leaf area and longest root length. Time of chitosan application once in the morning or evening gave the best result to increasing photosynthesis rate and stomatal conductance. Chitosan has the potential as a biostimulant for plant growth and is effective in increasing the physiological activity of plants.]]>
<![CDATA[Yield Performance of Short-Stem Promising Mutant Lines of Mentik Wangi Rice Induced by 200 Gy Gamma Irradiation ]]>
Fitria Roviqowati; Desfinenda Calistarajni; Parjanto Parjanto; Edi Purwanto; Ahmad Yunus
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v4i1.106011
<![CDATA[The demand for rice in Indonesia continues to increase but production has decreased. Efforts that can be made are the use of local varieties of mentik wangi rice. Mentik wangi rice has the disadvantages of long life, high stems, and low crop production. As a solution, plant breeding is carried out in the form of mutation by giving gamma irradiation which can improve unwanted genetic traits and produce superior varieties with the dose used of 200 gray. This study aims to evaluate the yield potential of promising mutant strains with 200 gray gamma irradiation on mentik wangi rice and get mutant strains that have superior criteria for short-stemmed, short-lived, and high-yielding. This research was conducted in the rice field of Ngampel Hamlet, Gentungan Village, Mojogedang, Karanganyar, Central Java. The research was conducted in June-October 2023. The design used was a Randomized Complete Group Design (RCBD) with a single factor, namely strains consisting of 14 mutant strains and 1 control strain with 3 repetitions. The results of observations were analyzed by Analysis of Variance (ANOVA) at the 5% level and continued with Duncan Multiple Range Test (DMRT) at the 5% level. All tested strains produced the expected superior criteria, namely short stem, short age, and high productivity.]]>
<![CDATA[Genetic Variability and Trait Correlations of Melon (Cucumis melo L.) Genotypes ]]>
<![CDATA[Heru Anggara]]>;
<![CDATA[Dicky Marsadi]]>;
<![CDATA[Listihani Listihani]]>
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 5, No 2 (2025) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v5i2.113228
<![CDATA[Melon (Cucumis melo L.) is an important horticultural crop. Melon breeding programs require comprehensive information on genetic variability and selection-related traits. Genetic variability and correlations among plant characters are essential parameters for effective selection in the S2 Population. This study aimed to evaluate the genetic variability of several melon genotypes and to analyze correlations among plant characters as a basis for selection. The experiment was conducted using fourteen melon genotypes derived from selfed populations at the S₂ generation. The study employed a randomized complete block design with a single factor, consisting of 14 S₂ melon genotypes and four replications. The experimental unit is one individual plant in a polybag. The Observations of qualitative traits indicated the presence of variation within the genotypes for fruit rind color and fruit flesh color. Genotypes G2 exhibited the greatest fruit length (13.47 cm) and fruit diameter (11.95 cm). Genotypes G15 showed the highest total soluble solids content (150 Brix), while genotypes G7 had the thickest fruit flesh (3.25 cm). Broad-sense heritability estimates of the observed traits ranged from low to moderate. The traits of fruit length, fruit diameter, and fruit weight exhibited moderate broad-sense heritability (>45%). They were positively and significantly correlated with fruit weight, indicating that these traits can be used as selection criteria for fruit weight in subsequent plant breeding programs.]]>
<![CDATA[DNA Damage in Prader Willi Syndrome Against Obesity ]]>
Diennisa Mursalin
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v3i2.77619
<![CDATA[Prader Willi Syndrome is a multisystem genetic disorder caused by deletions of paternal chromosomes (75%), maternal chromosomal dysomi (25%), and translocations (1%). This syndrome has a main characteristic, namely the state of obesity in individuals sufferers where. This is due to deficiency of Growth Hormone levels due to central hypothalamic-limbic disorders that occur in this syndrome. This syndrome has a characteristic course of obesity in every phase of life. The obesity that is characteristic of this syndrome causes diverse complications because it causes DNA damage through chronic inflammation caused by an increase in pro-inflammatory cytokines due to the buildup of adipose tissue. Thus, individuals with PWS can experience various metabolic diseases that can increase their morbidity and mortality.]]>
<![CDATA[Isolation of Lactic Acid Bacteria from Soil and Its Antifungal Activity Against Aspergillus flavus ]]>
<![CDATA[Athiyyah Jihan Tifani]]>;
<![CDATA[Tjahjadi Purwoko]]>;
<![CDATA[Ari Susilowati]]>
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v5i1.109898
<![CDATA[Food stocks, such as grains, fruits, and vegetables are vulnerable to spoilage by Aspergillus flavus, which typically controlled using chemical preservatives. Concerns about the health impact of these chemicals highlight the need for safer alternatives, such as biopreservatives. Lactic acid bacteria (LAB) are Generally Recognized as Safe (GRAS) microorganisms and have potential application as biopreservative agent, because LAB produced various metabolites with antifungal activities. LAB could be found in nutrient-rich environments, including rhizosphere soil and poultry house soil due to the nutrient residues for growth. The aims of this study were to isolate LAB from soils and to evaluate their antifungal activity with a focus on potential applications as biopreservatives. The LAB isolates from soil were characterized and screened for antifungal activity using the dual culture method. The LAB isolates inhibited A. flavus growth with the largest growth inhibition was showed by LAB isolate 1 that isolated from poultry house soil (17.65%). However, statistics analysis showed that fungal growth inhibition by LAB isolates were not significantly difference (p>0,05). Although the result was not statistically significant, all LAB isolates significantly inhibited conidia development. This showed potential application of LAB isolates as biopreservative agent. Keywords: antifungal, Aspergillus flavus, biopreservative, food stock, lactic acid bacteria]]>
<![CDATA[APPLICATION OF BENZYL ADENINE AND IAA ON THE GROWTH OF BANANA BARANGAN SUBCULTURE (Musa acuminata L.) ]]>
<![CDATA[Andriyana Setyawati]]>;
<![CDATA[Samanhudi Samanhudi]]>;
<![CDATA[Edi Purwanto]]>;
<![CDATA[Retna Bandriyati Arniputri]]>;
<![CDATA[Kevin Putra Setyawan]]>;
<![CDATA[Muhammad Aji Cahyadi]]>
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v4i2.108497
<![CDATA[Barangan banana is a banana variety that has high economic value, but its production is still carried out conventionally, resulting in limited barangan bananas on the market. The use of tissue culture methods can quickly produce high-quality, disease-free plants regardless of seasonal conditions. The aim of this research is to determine the effect of providing the best ZPT IAA and BA for the growth of the Barangan banana planet. The research was conducted in August-October 2023 at the Biotechnology and Plant Physiology Laboratory, Sebelas Maret University. This research used a two-factor Completely Randomized Design (CRD) with 6 replications. The first factor was the use of ZPT Indole Acetic Acid (concentrations 0, 0.5, 1, and 1.5 ppm and the second factor was the use of ZPT Benzyl Adenine (0, 0.5, 1, 1.5 ppm). The variables observed were root emergence time, number of roots, root length, time of leaf emergence, number of leaves, time of shoot emergence, number of shoots, and height of shoots. The observed data were analyzed using Analysis of Variance (ANOVA) with a 5% level test and if there was a significant difference, it was continued with Duncan's Multiple Range Test (DMRT) at the level 5%. The results showed that giving 0.5 ppm IAA increased root length; 0.5 ppm IAA was given optimally for root formation; and 1 ppm IAA increased the shoot height of the Barangan banana plnalet. Giving BA 0.5 ppm root number; BA 1, 5 ppm produces the highest number of leaves; BA 1 ppm increases shoot height of barangan banana plantlets.]]>
<![CDATA[A review on the updated checklists of the amphibians and reptiles of Pakistan: Present and prehistoric ]]>
Amtyaz Safi; Hans-Volker Karl
<![CDATA[ Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024) ]]>
Publisher : <![CDATA[Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS ]]>
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DOI: 10.20961/jbb.v4i1.106964
<![CDATA[Recent herpetological collections have added several new taxa to the previously compiled herpetofauna of Pakistan. This paper presents annotated and updated checklists of 243 species, 104 genera, and 27 families of extant herpetofauna, including 24 anuran amphibian species, belonging to 4 families. In comparison, the reptilian fauna consists of 219 species/subspecies, including 2 species of crocodiles, 15 species of turtles and tortoises, 119 species of Sauria, and 83 species of snakes belonging to 23 families. Four species of amphibians and 33 species of reptiles are endemic to Pakistan. Separate checklists of prehistoric reptiles (50 taxa) for both dinosaurs (26 taxa) and non-dinosaurian taxa (24 taxa) are also presented here. This review article is based on previous literature on the herpetofauna of Pakistan. It will serve as a baseline for future work related to the herpetofauna of Pakistan, which is essential in biodiversity research.]]>
