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Journal of Food and Pharmaceutical Science
Published by Universitas Gadjah Mada
ISSN : 20897200     EISSN : 23390948     DOI : https://doi.org/10.22146/jfps.581
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology
FOCUS AND SCOPE Journal of Food and Pharmaceutical Sciences offers scientists, researchers, and other professionals to share knowledge of scientific advancements. The journal will publish original research articles, review articles, short communication, and letter to editor. The area of focus should cover all aspects of food and pharmaceutical sciences. The range of topics covered in the journal include: New Horizons in Food Research Food Chemistry Integrated Food Science Health, Nutrition, and Food Food Engineering, Materials Science, and Nanotechnology Toxicology and Chemical Food Safety Food Microbiology and Safety Drug Discovery Computational Chemistry and Molecular Modeling Pharmaceutical Biotechnology and Protein-Peptide Chemistry Pharmaceutics, Biopharmaceutics, Drug Delivery, and Pharmaceutical Technology Pharmaceutical Nanotechnology Pharmacokinetics, Pharmacodynamics, and Drug Transport Metabolism Analytical and Bioanalytical Chemistry Pharmaceutical Chemistry Natural Medicine and Nutraceutical Chemical Processing of Pharmaceuticals including Crystallization, Lyophilization, and Chemical Stability of Drugs Immunology, Biochemistry, and Cell and Molecular Biology
Arjuna Subject : Pharmacology, Toxicology and Pharmaceutics - Ilmu Farmasi
Articles 254 Documents
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Influence of Stearic Acid and Triethanolamine on the Physical Properties and Antibacterial Efficacy of Ocimum basilicum L. Anti-acne Cream against Staphylococcus epidermidis Nurcahya, Salsabila; Pranata, Yovi; Pratamarta, Meliasi Nora; Hidayati, Nurul; Setiawansyah, Arif
Journal of Food and Pharmaceutical Sciences Vol 13, No 4 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.25043

Abstract

Acne vulgaris represents a significant dermatological concern, with Staphylococcus epidermidis identified as a key pathogenic contributor. While Ocimum basilicum L. (basil) leaves contain bioactive compounds including flavonoids, eugenol, and tannins that demonstrate promising antibacterial properties, the successful translation of these natural antimicrobials into effective topical formulations remains critically dependent on appropriate excipient selection. Despite the growing interest in botanical-based acne treatments, there exists a significant knowledge gap regarding how emulsifying agents, particularly stearic acid and triethanolamine (TEA), influence both the physical stability and therapeutic efficacy of herbal cream formulations. This study investigated the effects of varying concentrations of stearic acid and TEA on the physical characteristics, stability, and antibacterial activity of basil leaf extract cream formulations against S. epidermidis ATCC-12228. Extracts obtained via maceration in 96% ethanol were incorporated into cream formulations (F0–F4), which were subsequently evaluated for organoleptic properties, homogeneity, spreadability, adhesion, pH, stability using a thermal cycling test, and antibacterial activity via disc diffusion. Stability assessment revealed notable differences across formulations. Although all formulations maintained consistent pH values and exhibited uniform microscopic homogeneity after cycling, variations in spreadability and adhesion indicated differing degrees of structural stability. Formulations F3 and F4 showed minimal changes across cycles, demonstrating superior resistance to thermal stress, whereas F0 exhibited significant instability in both spreadability and adhesion. Antibacterial testing showed that the formulation containing 20% basil extract (F4) produced the largest inhibition zone (11.83 ± 0.77 mm). Beyond its higher extract content, F4’s superior antibacterial performance is attributed to its more stable structural matrix, which likely enhanced the release and bioavailability of active phytochemicals such as eugenol and flavonoids, thereby promoting more efficient diffusion into the agar medium. Overall, the findings demonstrate that stearic acid and TEA concentrations substantially influence both the physical stability and antibacterial efficacy of basil-based cream formulations. The optimal stability and enhanced antimicrobial activity observed in F3 underscore their potential as promising candidates for topical anti-acne product development.
Integration of Molecular Docking in the Identification of Natural Antioxidants: Interaction Study of Jackfruit Leaf Flavonoids with NADPH:FMN Oxidoreductase Muhammad Andre Reynaldi; Meri Ropiqa
Journal of Food and Pharmaceutical Sciences Article In Press 2026
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.26236

Abstract

Oxidative stress plays a critical role in the progression of various degenerative diseases through increased production of reactive oxygen species (ROS), driven in spart by the activity of redox related enzymes such as NADPH:FMN oxidoreductase. Bioactive compounds from jackfruit leaves are known to possess antioxidant potential, yet their molecular mechanisms against specific enzymatic targets remain insufficiently elucidated. This study aimed to evaluate the potential interaction of jackfruit leaf flavonoids morin, oxyresveratrol, and artocarpin with NADPH:FMN oxidoreductase using molecular docking analysis. The 1BKJ protein structure was prepared following standard protocols, and all ligands were optimized prior to performing redocking for method validation. AutoDock Vina 1.2.7 was employed with a 20×20×20 Å grid box area. Redocking produced an RMSD of 0.1469 Å, confirming the reliability of the docking parameters. Docking results revealed that morin (–7.848 kcal/mol) and oxyresveratrol (–7.577 kcal/mol) exhibited stronger binding affinities compared with vitamin C (–5.713 kcal/mol) and artocarpin (–5.577 kcal/mol). The dominant interactions involved Arg15, Arg169, Tyr128, Tyr199, and Tyr200, residues that contribute to the stabilization of ligand protein complexes in silico and may be located near functionally relevant regions associated with redox activity. These findings suggest that jackfruit leaf flavonoids may serve as promising candidates for further investigation as potential modulators of redox related enzymes based on predictive in silico evidence.
Formulation of Theophylline Sustained-Release Tablets with a Combination of Eudragit RS 100 and Lactose Trisnaningtyas, Meysiska; Ikasari, Endang Diyah; Intan Martha Cahyani
Journal of Food and Pharmaceutical Sciences Article In Press 2026
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.26837

Abstract

Sustained-release (SR) delivery of Theophylline is pharmaceutically essential due to its narrow therapeutic index and the need to maintain controlled plasma exposure. This study aims to investigate the release kinetics and matrix-modifying roles of Eudragit RS 100 and lactose in three SR tablet formulations of Theophylline. Tablets were produced with the wet granulation method and evaluated for physicochemical properties, dissolution behavior according to USP specifications, and kinetic model fitting. The physicochemical evaluation revealed clear differences among formulations. F-1 exhibited the highest hardness (10.48 ± 0.10 kg/cm²) but excessive friability (2.40 ± 0.01%), whereas F-2 and F-3 showed lower hardness values (8.25 ± 0.03 and 8.03 ± 0.02 kg/cm²) with acceptable friability (0.32 ± 0.03% and 0.34 ± 0.03%). Granule flow properties improved progressively from F-1 to F-3, as indicated by reduced Carr’s Index (16.17 ± 0.16 to 11.58 ± 0.32%) and Hausner Ratio (1.193 to 1.131), accompanied by increased flow rates (12.12 ± 0.03 to 14.48 ± 0.53 g/s) (p < 0.05). These physicochemical differences were reflected in dissolution behavior and drug-release kinetics, confirming the matrix-modifying effects of Eudragit RS 100 and lactose. Kinetic modeling demonstrated formulation-dependent release mechanisms: F-1 showed the highest correlation with the zero-order model (R² = 0.9858), suggesting a near constant drug-release rate, whereas F-2 exhibited the best fit to a second-order model (R² = 0.9635), indicating concentration-dependent release. In contrast, F-3 was optimally described by the Korsmeyer–Peppas model (R² = 0.9545), consistent with anomalous transport governed by combined diffusion and polymer relaxation.
Analysis of Antioxidant Activity of Ethanol Extracts of Cocoa Fruit Peel (Theobroma cacao L.) and Arabica Coffee Fruit Peel (Coffee arabica L.) with Maceration and Reflux Extraction Method Indah, Indah; Lestari, Dina; Lasenda, Shintia Puja; Sitindaon, Rina S E
Journal of Food and Pharmaceutical Sciences Vol 13, No 3 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.20800

Abstract

Cocoa and coffee are well-known commodities in the world, including Indonesia. The seeds of cocoa and coffee are often utilized. Cocoa beans are usually processed into chocolate products, while coffee becomes a beverage product. Utilization of cocoa and coffee beans, and fruits produces waste, namely the skin. The more cocoa and coffee production, the more fruit skin waste. One of the efforts to utilize fruit skin waste is by extracting the antioxidant content from it. The choice of extraction method is important to produce extracts with the maximum amount of extract. Therefore, a study was conducted on the comparison of maceration and reflux extraction methods in determining the highest level of antioxidant activity. Cocoa and coffee fruit peels were extracted using ethanol, and the extracts were tested for antioxidant activity using DPPH with ascorbic acid as a positive control using UV-Vis spectrophotometry. The results of the antioxidant activity test conducted with the DPPH method showed that the IC50 value of macerated cocoa fruit peel extract was 5.440 ppm, and coffee was 62.99 ppm the reflux extract of cocoa fruit peel was 4.999 ppm, and coffee was 61.68 ppm. This shows that the maceration and reflux methods of cocoa can be categorized as very strong antioxidants and strong arabica coffee. from the two extraction methods of this study, it is concluded that there is no significant difference between maceration and reflux.
Development of Self-Nano Emulsifying Drug Delivery System (SNEEDS) Containing Hibiscus sabdariffa L. Extract, an Anticancer against T47D Cells, as a Co-Chemotherapy of Cisplatin Nurani, Laela Hayu; Efiana, Nuri Ari; Wahyuningtyas, Nurma; Edityaningrum, Citra Ariani; Indratmoko, Septiana; Guntarti, Any; Ma’ruf, Muhammad
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.21378

Abstract

The first-line chemotherapy drug for breast cancer is cisplatin. However, it shows a high incidence of resistance. Therefore, Hibiscus Sabdariffa L (HS), containing strong anticancer compounds, was developed using a self-nano-emulsifying drug delivery system (SNEDDS), potentially as cisplatin co-chemotherapy. This study aimed to develop the HS SNEDDS providing anticancer activity against T47D breast cancer cells. The methods consist of the development of HS SNEDDS (F1-F7) with various surfactant and co-surfactant concentrations, followed by characterization of HS SNEDDS. The selected formulation was evaluated regarding the cytotoxicity on T47D cells and selectivity on Vero cells. The results showed that F7, as a selected formulation, indicated a transmittance, globule size, PI, zeta potential, and emulsification time of 97.80%, 15.68 ± 0.19 nm, 0.12 ± 0.01, -8.05 ± 1.88 mV, and 24.76 ± 0.29 seconds, respectively. Furthermore, according to the data of toxicity and selectivity studies, HS SNEDDS was proven to enhance the toxic properties of HS extract on T47D cells and be safe on normal cells. In conclusion, HS SNEDDS providing the required characteristics could be obtained, and potentially be used as a co-chemotherapy of cisplatin, showing the cytotoxic effect on T47D breast cancer cells.
Anti-Inflammatory Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid Derived from Patin Fish Oil on Diabetic Nephropathy: A Bioinformatics Study Purnomo, Doddy Aditya; Awidarta, Kevin; Nugroho, Agung Endro; Rohman, Abdul
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.22899

Abstract

Diabetic nephropathy is a severe complication of diabetes mellitus with a significant global impact on end-stage renal disease. Fish-derived fatty acids show promise in inflammatory disorders, but their mechanisms in diabetic nephropathy remain unclear. This study used network pharmacology and molecular docking to investigate the therapeutic targets of EPA and DHA from Patin fish oils. Potential targets of EPA and DHA were retrieved from the Swiss Target Prediction, SEA, and SuperPRED databases, identifying 160 and 185 targets, respectively. Notably, 37 and 62 of these targets overlapped with DN-related targets from GeneCards, DisGeNET, and OMIM. Protein-protein interaction (PPI) network analysis revealed hub genes, including PPARG, TLR4, and TP53, as critical mediators. Gene Ontology (GO) enrichment analysis revealed involvement in biological processes such as collagen metabolic process for EPA and regulation of inflammatory response for DHA, while KEGG pathway analysis highlighted the modulation of PPAR signaling, the renin-angiotensin system, and the AGE-RAGE signaling pathway. Molecular docking confirmed favorable binding affinities of EPA and DHA to key targets such as PPARG (-8.04 kcal/mol for DHA) and PPARD (-8.11 kcal/mol for EPA). These findings suggest that EPA and DHA may mitigate DN-associated inflammation through multi-target and multi-pathway interactions, positioning them as potential supplementary therapeutic agents.
Characteristics of Pie Crusts Made from Modified Cassava Flour (MOCAF) and Red Bean Flour Siregar, Erpiani; Ayu, Dewi Fortuna; Putri Elvis, Shelby Julia; Diana, Ayu; Rahmayuni, Rahmayuni
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.24058

Abstract

The development of bakery products has increased the demand for wheat flour alternatives, especially for individuals with dietary restrictions. This study aims to investigate the characteristics of pie crusts made from MOCAF (modified cassava flour) and red bean flour at various ratios. The experimental method used was a completely randomized design (CRD) with four treatments: MK1 (90% MOCAF, 10% red bean flour), MK2 (85% MOCAF, 15% red bean flour), MK3 (80% MOCAF, 20% red bean flour), and MK4 (75% MOCAF, 25% red bean flour). The parameters evaluated included moisture content, ash content, protein content, fat content, and sensory testing (color, aroma, taste, and crumb texture) using descriptive and hedonic tests. The results of the study showed that increasing the proportion of red bean flour significantly affected the sensory characteristics and nutritional content of pie crusts. MK4, with a ratio of 75% MOCAF and 25% red bean flour, achieved the highest hedonic scores for color (3.63), taste (3.50), aroma (3.86), and crumbly texture (4.18), which were most preferred by the panelists. Proximate analysis revealed that MK4 had a moisture content of 4.21%, ash content of 1.88%, protein content of 9.94%, and fat content of 26.09%. In conclusion, the addition of red bean flour to the pie crust formulation with MOCAF improves sensory quality and nutritional value, making it a suitable alternative for healthier bread products.
Ethnopharmacology Analysis of Antidiabetic Medicinal Plants from Menoreh Hills: Linking Local Knowledge to Pharmacological Evidence Nur Arib, Faisal; Kintoko, Kintoko; Sulistyani, Nanik; Akrom, Akrom
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.24603

Abstract

Abstract: The prevalence of type 2 diabetes mellitus (T2DM) has been increasing worldwide and in Indonesia. Documenting locally utilized antidiabetic plants may inform evidence-based phytotherapy. This study mapped antidiabetic medicinal plants in Samigaluh (Menoreh Hills) and prioritized candidates by linking community knowledge with pharmacological data. Semi-structured interviews and observation were conducted between July and August 2025, and involved ten informants selected through purposive and snowball sampling. Quantitative ethnobotanical indices (Use Value, Relative Frequency of Citation, Fidelity Level, Plant Part Value) were calculated, followed by a PubMed-based narrative review. The Zingiberaceae family was predominant (seven species); rhizomes were the most utilized (39.58%), followed by stems (25.00%) and leaves (22.92%). Vernonia amygdalina was prioritized for diabetes-specific consensus (Fidelity Level 100%, Relative Frequency of Citation 0.7), and Curcuma mangga for its high usage and popularity (Use Value 1.3, Relative Frequency of Citation 0.6). Pharmacological evidence indicates that V. amygdalina exhibits antidiabetic effects through the inhibition of α-amylase and α-glucosidase, enhancement of GLUT4, and suppression of gluconeogenesis, supported by its antioxidant and anti-inflammatory properties, while C. mangga demonstrates hypoglycemic activities and β-cell protection, along with anti-inflammatory, antioxidant, and immunostimulant activities as supportive mechanisms. Local knowledge thus aligns with mechanistic findings, supporting a complementary pairing of V. amygdalina and C. mangga for future antidiabetic combinations; further validation through in vitro and in vivo studies is recommended.
Evaluation Antibacterial Activity of Rosary Pea (Abrus precatorius L.) Leaf Extract against the Growth of Staphylococcus aureus and Shigella sonnei Shari, Amalia; Syahfitri, Alda Bunga
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.24861

Abstract

Bacteria are major contributors to infectious diseases, including Staphylococcus aureus and Shigella sonnei. Although antibiotics remain the primary treatment, prolonged use can lead to antimicrobial resistance, highlighting the need for alternative agents capable of inhibiting pathogenic bacteria. Scientific validation of medicinal plants is crucial for complementing traditional knowledge in bioprospecting efforts. The Rosary pea (Abrus precatorius L.) is one such plant with potential antibacterial properties. This study evaluates the inhibitory activity of Abrus precatorius L. leaf ethanol extract against S. aureus and S. sonnei at concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%. The extract demonstrated optimal activity at 100%, exhibiting moderate inhibition against S. aureus and weak inhibition against S. sonnei. These findings underscore the potential of Abrus precatorius L as a source of antibacterial compounds and provide a foundation for developing improved formulations with enhanced antibacterial efficacy.
Production of Solid Soap from Arabica Coffee Grounds (Coffea arabica L.) with Antibacterial Properties Sanjiwani, Ni Made Sukma; Ariani, Komang; Sunadi Putra, I Made Agus; Rahadi, I Wayan Surya; Mirah Mariati, Ni Putu Ayu; Sudiarsa, I Wayan; Udayani, Ni Nyoman Wahyu
Journal of Food and Pharmaceutical Sciences Vol 14, No 1 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.25035

Abstract

Coffee shops are now a favourite hangout for people of all ages, so many entrepreneurs are developing coffee shop businesses because of their high profit potential. Arabica coffee is the type of coffee most commonly used in coffee shops. The coffee-making process produces waste in the form of coffee grounds. Coffee grounds can be used as an ingredient in beauty products such as soap. Soap is the result of saponification, which is a reaction between a base or alkali and fatty acids, which acts as a skin cleanser. This study aims to examine the presence of secondary metabolite compounds in Arabica coffee grounds (Coffea arabica L.), test the physical quality of solid soap made from Arabica coffee grounds (C arabica L.), and assess the soap's ability to inhibit the growth of Staphylococcus aureus bacteria. Arabica coffee grounds were first analysed through qualitative phytochemical screening tests using test tubes and various reagents, then formulated into solid soap with different concentrations, namely 4%, 7%, and 9%. After that, physical properties testing and antibacterial activity evaluation against Staphylococcus aureus were carried out using the disc diffusion method. The research results data were presented using descriptive analysis. The tests revealed that Arabica coffee residues contain secondary metabolites in the form of alkaloids, flavonoids, tannins, saponins, and triterpenoids. Solid soap made from Arabica coffee grounds meets the physical quality test standards in accordance with SNI 3532:2021 and that solid soap made from Arabica coffee grounds with a percentage of 7% (formula 2) and 9% (formula 3) has the potential to inhibit the activity of Staphylococcus aureus bacteria with a moderate category. It can be concluded that Arabica coffee grounds contain secondary metabolites such as alkaloids, flavonoids, tannins, saponins, and triterpenoids. Solid soap with Arabica coffee grounds as an ingredient meets the physical quality requirements in accordance with SNI 3532:2021. Soap formulas with a coffee grounds concentration of 7% (F2) and 9% (F3) have the potential to inhibit the growth of Staphylococcus aureus with moderate efficacy.

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