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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 16 Documents

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Search results for , issue "Vol 36, No 1 (2018): Juni" : 16 Documents clear

Nano Spray TRISWHEAT (Teripang Super Wound Healing Agent) Penyembuh Luka Diabetes Mellitus yang Terinfeksi Bakteri MRSA (Methicillin Resistant Staphylococcus aureus) dengan Ekstrak Teripang Nada Hanifah; Yusuf Farid Achmad; Mellya Permatasari; Marista Kurniati; Ditya Tiwi Syafira; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Sea Cucumber (Stichopus sp) belongs to Holothuroidea family. Sea Cucumber has lot of uses in the medicine sector, such as: for wound healing, anti-biotic, anti-bacteria, anti-tumor, and anti-coagulation. Diabetes mellitus is a disease characterized by high blood sugar levels with metabolic disorders. This disease can cause wounds called gangrene This research has purpose to prove the influence of giving nano spray of sea cucumber extract within 14 days. In the in vitro examination found 40% concentration of sea cucumber extract which is effective to resist the growth of Methicillin Resistant Staphylococcus aureus bacteria and examined to wistar mouse which are already diabetic inducted with Streptozotocin (STZ) and hurt with punch biopsy with the diameter of 0,8 cm on its back and the wound is infected with bacteria which is intradermal injected. Divided into 3 groups : (K-) no medical treatment, nano spray of sea cucumber extract (T), Nano spray penicillin (K+) to wistar mouse towards the speed of diabetic mellitus wound healing which is infected by Methicillin Resistant Staphylococcus aureus bacteria. Parameter used to measure the rate of wound healing is wound size and hitopathology examination.After it’s regularly given the medicine, the result of nano spray sea cucumber extract 40 % concetration category treatment on wound healing process the diameter of wound on the 3rd day 0.8 cm , on the 7th day 0.4 cm and on the 14th day wound fully recover according to macros (wound size) and histopathology examination shows faster and better healing compared to treatment category of nano spray penicillin and control marked by score cell and lymphocyte in a small quantity.

Rekayasa Konsentrat Bekatul dengan Tepung Jantung Pisang Dapat Meningkatkan Berat Badan dan Menurunkan Kadar Kolesterol Kambing Dina Deviana; Nur Prabowo Dwi Cahyo; Dwi Retna Kumalaningrum; Widya Ayu Kusuma; Fahlevi Lailiyah; Muhammad Thohawi Elziyad Purnama
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Tujuan: tujuan dari penelitian ini yaitu untuk meningkatkan berat badan dan menurunkan kadar kolesterol pada kambing. Metode Penelitian: metode penelitian yang digunakan yaitu dengan pemberian feed additive pakan konsentrat bekatul dengan tepung jantung pisang menggunakan metode rancangan acak lengkap (RAL). Penelitian ini menggunakan 5 perlakuan dengan 4 pengulangan, jumlah hewan coba yaitu kambing 20 ekor. Kambing yang kita gunakan berjenis kelamin jantan dengan umur 1,5 tahun. Pengumpulan data diambil dari peningkatan berat badan dan kadar kolesterol kambing. Hasil: pemberian feed additive konsentrat bekatul dengan tepung jantung pisang dapat meningkatkan berat badan dan menurunkan kadar kolesterol pada kambing. Nilai keberhasilan dengan skala P<0.05, menunjukan adanya perbedaan nyata pada tiap perlakuan yang dilakukan. Perlakuan terbaik yang dilakukan pada penelitian ini yaitu pada P2 dengan kenaikan berat badan 2,95 ons/hari dan kadar kolesterol dalam dua minggu turun sebanyak 87,75. Kesimpulan: pada penelitian ini dapat disimpulkan bahwa pada tiap perlakuan memiliki perbedaan nyata untuk kenaikan berat badan dan penurunan kadar kolesterol kambing. Dapat disimpulkan juga bahwa penambahan pakan konsentrat bekatul dan tepung jantung pisang dapat meningkatkan berat badan dan menurunkan kadar kolesterol pada kambing.

Dekontaminasi Mycobacterium avium subspecies paratuberculosis pada feses menggunakan beberapa jenis desinfektan Ika Suharti; Ni Luh Putu Ika Mayasari; Fachriyan Hasmi Pasaribu
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Paratuberculosis or Johne’s Disease is a granulomatous enteritis chronic disease of domestic and wild ruminants caused by infection of Mycobacterium avium subspecies paratuberculosis. The disease commonly infects dairy cattle with clinical signs of chronic diarrhea, decreasing body weight, low milk production, oedema, anemia and occasionally infertility. The basic procedure in order to control Paratuberculosis in farms is to do a good and proper handling of animal faecal. Disinfection of animal environments such as pens, faecal, sewerage and sewage are important in prevention of transmission of this disease. The purpose of this research is to determine specific disinfectan and dosage for Mycobacterium avium subspecies paratuberculosis decontamination in cattle feces so it can be applied as disease control measures. Cow's feces were contaminated with MAP 105CFU/ml and treated with ammonium quartener, phenolic and formaldehyde disinfectant doses 10%, 15% and 20%. The effectiveness of the disinfectant was tested based on MAP identification using Löwenstein-Jensen culture medium and nested Polymere Chain Reaction(PCR). The results showed 15% and 20% doses of formaldehyde disinfectants efective to decontaminate Mycobacterium avium subspecies paratuberculosis in catle feces.

Kajian Equine Infectious Anemia pada Kuda Impor di Bandar Udara Internasional Soekarno-Hatta Marlefzena Marlefzena; Sri Murtini; Joko Pamungkas
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Equine Infectious Anemia (EIA) disease is caused by EIA Virus (EIAV) from genus Lentivirus, subfamily Orthoretrovirinae, family retroviridae. This virus causes a persistent infection and potentially fatal in Equidae. There is no scientific literature yet for this disease in Indonesia. In order to determine this disease in our country, it is necessary continuing laboratory detection in imported Equidae and conducted further surveillance in Equidae throughout Indonesia. This research is aimed (1) to detect of any antibodi titre of EIAV in imported horse, (2) To get information about the relationship between laboratory result with the secondary datas of origin country. The EIAV antibody detected by indirect enzyme-linked immunosorbent assay (indirect ELISA). The secondary datas were collected from Health Certificate of origin country, World Animal Health Information System (WAHIS) Interface OIE and literatures. Total of 133 blood samples were collected from imported horse, ex-imported horse and lokal breed horse. The result showed that all of imported horse, ex-imported horse and lokal breed horse were negative antibody against EIAV. The initial information on Equine Infectious Anemia indicated that health requirement in imported horse has fulfilled, so that imported horse was free from EIAV.

Perbandingan Dua Desinfektan dalam Mengeliminasi Virus Avian Influenza H5N1 pada Telur Tetas Umar Suryanaga; Retno D. Soejoedono; Ni Luh Putu Ika Mayasari
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Avian Influenza (AI) is a zoonotic viral disease in birds which demands priority on control and measures. Spread of AI virus can occur directly or indirectly. The use of disinfectant and handling of hatching egg waste into one of the actions that must be applied in hatchery to control the spread of AI virus. This research aim to compared two types of desinfectant in eliminating AI virus. The research was designed into 6 groups. Group I was SAN (Specific Antibody Negative) eggs as untreated negative control, group II was SAN egg treated by fumigation using potassium permanganate (KMnO4) and formalin in room temperature for 20 minutes, group III was SAN eggs soaked in benzalkolnium chloride (BKC) in room temperature for 30 seconds, group IV was SAN contaminated by AI H5N1 virus and fumigated by potassium permanganate and formalin in room temperature for 20 minutes, group V was SAN eggs contaminated by AI H5N1 virus and then soaked in benzalkonium chloride in room temperature for 30 second, and group VI was SAN eggs contaminated by AI H5N1 virus in room temperature for 10 minutes as positive control. AI H5N1 virus detection was done by using RT-PCR (Reverse Transcription Polymerase Chain Reaction) and confirmed by isolation in Embyronated Chicken Egg. The result of this research showed that the use of potassium permanganate and formalin disinfectant gave little better performance compared to benzalkoniun chloride in eliminating AI H5N1 virus on hatching eggs.

Daya Ovisidal dan Larvisidal Infusa Daun Bambu Apus (Gigantochloa apus)Terhadap Haemonchus Contortus Secara in Vitro Budi Purwo Widiarso; Wisnu Nurcahyo; Kurniasih Kurniasih; Joko Prastowo
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Gastrointestinal Nematodes are one among cause decrease production efificiency in goat and sheep in Indonesia, especially parasite of abomasum namely Haemonchus contortus. This parasite causes disease of Haemonchosis causing anemia, and causes mortality to young goat or sheep. Loss of economics caused by high morbidity and mortality this parasite so that can improve the expense of prevention and medication of this disease. This research aim was to evaluate ovicidal and larvicidal apus bamboo leaf infusion (Gigantochloa apus) against Haemonchus contortus by in vitro. Examination of apus bamboo leaves infusion to seven concentrations namely : 1.2; 1.0; 0.8,; 0.6; 0.4; 0.2;0.1; 0.05 and 0 mg / ml as negative control. Positive control uses Albendazole with concentration 0,025 mg / ml. The highest concentration 1.2 mg / ml apus bamboo leaves infusion inhibited 92.35% egg hatching ( LC 0.49 mg / ml ) and have larvicidal activity equal to 90% ( LC50 0,47mg / ml). The concentration 1.2 mg / ml also have similar effect with positive control which use Albendazole. Phytochemical apus bamboo leaf analysis of the highest concentration revealed the presence tanin condensed, saponins, and alkaloids.

Deteksi Hemaglutinin, Hemolisin dan Koagulase Secara Fenotipik dan Genotipik pada Staphylococcus aureus Isolat Asal Broiler Khusnan Khusnan; Dwi Kusmanto
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is a bacterium causing diseases in animals and human. Staphylococcus aureus in broilers cause septicemia, tendosinovitis, dermatitis, endocarditis, wound infections, arthritis and bumblefoot. In this research, 24 isolates of Staphylococcus aureus from broiler were characterized of its virulent factors including the presence of haemagglutinin, ability to agulate the plasma in tubular coagulase test as well presipitate formation in clumping factor test, and haemolysis types. By polymerase chain reaction (PCR) were genotypically detected genes coa, clf, hlaA, and hlaB. All of the isolates (100%) had haemagglutinin, capable to agglutinate and precipitae of rabbit plasma. All isolates could lyse sheep red blood cells with the type of α-hemolysis (45.8%), β-hemolysis (50.0%) and γ-haemolysis (4.2%). Genotypically, all isolates (100%) had coa and clf genes, hlaA gene 7(0.8%) and hlaB gene (29.2%).

Identifikasi Escherichia coli dan Salmonella spp pada Karkas Sapi di Rumah Potong Hewan di Banyuwangi dan Resistensi Terhadap Antibiotika Faisal Fikri; Muhammad Thohawi Elziyad Purnama; Amung Logam Saputro; Iwan Sahrial Hamid
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Food borne disease can be transmitted through Escherichia coli and Salmonella spp contamination. The contamination of microorganisms with high pathogenic potentials on bovine carcasses results in food borne illness. The aim of this study was to identify multidrug-resistance of Escherichia coli and Salmonella spp on carcass samples that isolated from slaughter house in Banyuwangi. Samples were collected from district of Banyuwangi, Rogojampi, Genteng and Kalibaru. This study used cross sectional study with assumption of prevalence at 50% in each contaminant, confidence level 95% and standart of error at 10%. By the number of samples should reach 96 samples. The result showed that seven samples (7,3%) were positive Escherichia coli and none samples (0%) were positive Salmonella spp. The multidrug-resistance of Escherichia coli showed that Cephalotin (42,9%), Trimethoprim (14,3%) and Erythromycin (42,9%) whereas Ampicillin, Enrofloxacin, Chloramphenicol and Tetracycline were sensitive against Escherichia coli.

Perbandingan Kinerja Berahi dan Level Estradiol Kambing Kacang dan Kambing Nubian yang Diinduksi dengan PGF2α Mulyadi Adam; Raja Nurul Huda; Widya Zahara; Tongku Niswan Siregar; Sri Wahyuni; Cut Nila Thasmi; Rosmaidar Rosmaidar
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The experiment was conducted to compare estrous performance between and estradiol level kacang and Nubian goats estrous induction by prostaglandin F2 alpha (PGF2α). Eight female goats were divided into two groups, groups 1 (K1) consisted five Nubian goats and group 2 (K2) included three kacang goats with criterias as follows: age 1.5-3.0 years old, not pregnant, at least two months postpartum, and healthy clinically. All goats were estrous synchronized by double injection 7,5 mg PGF2α intramuscularly with 10 days interval. Goats that appeared estrus symptoms were mated naturally to a male goat. Determination of estrous intensity was performed using scoring method. Blood samples were taken at the first time the goats were mated (day 0). The measurement of estradiol concentration was done by enzyme linked immunosorbent assay (ELISA) method. Data of intensity, onset, duration, and estradiol level were analyzed using t test. The results showed that the rate of intensity, onset, duration, and estradiol level of estrous at KI vs KII were 2.08±0.52 vs 1.85±0.67, 56.00±34.11 vs 40.80±18.20 hours, and 24.00±26.15 vs 33.80±9.10 hours, and 103.51±42.49 vs 67.95±38.44 pg/ml (P>0,05). It can be concluded there was no difference of estrous performance and estradiol level between kacang and Nubian which estrous induction with PGF2α. The relationship between the intensity of estrus estradiol level in the Nubian goats was 0.995 while the kacang goats was 0.890.

Analisis Scanning Electron Microscope (SEM) Mikroarsitektur Daerah Metafisis Os Femur Tikus Putih (Rattus novergicus) Ovariohisterektomi dengan Pemberian Madu Lebah (Apis dorsata) Hutan Sumbawa Samsi Yordan; Abdullah Hasib; Muhammad Huda Ramadhan Ibrahim; Siti Nur Rohmah; Salsabilla Abani; Ira Sari Yudaniayanti
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aims of this study to determine the effect of honey bees (Apis dorsata) to prevent the decrease of femur density in ovariohysterctomized rats as animal model of osteoporosis by Scanning Electron Microscope (SEM). This study used 20 adult female white rats (Rattus norvegicus) with body weight 300 gr. Divided into 5 groups, negative control group (P0-), not in ovariohysterectomy, only given aquadest 1.5 ml/day ; Positive control group (P0 +), in ovariohysterectomy, only given aquadest 1.5 ml/day; Treatment group 1 (P1), in ovariohysterectomy and given honey 1 gram / kg BB + 1.5 ml aquadest; Treatment group 2 (P2), in ovariohysterectomy and given honey 2 grams / kg BB + 1.5 ml aquadest; Treatment group 3 (P3), in ovariohysterectomy and given honey 4 grams / kg BB + 1.5 ml aquadest. After 12 weeks, all rats were sacrified for removal of the femur and performed by SEM. Analysis of SEM showed that mice administered honey with a dose of 4 grams / kg BW (P3) showed bone density approaching negative control treatment (P0-), ie, ovariohysterectomized rats. P3 showed the most less bone porosity than other treatment groups. The largest and most uniformly distributed porosity was shown by the positive control group (P0 +). The best results with the smallest quantity and size of the porosity of femur is in P3 group. In conclusion, honey (Apis dorsata) can prevent the decrease in bone density of the femur by Scanning Electron Microscope (SEM).

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