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Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 796 Documents
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HUBUNGAN KONSENTRASI MALONDIALDEHIDA, GLUKOSA DAN TOTAL KOLESTEROL PADA TIKUS PUTIH YANG DIINJEKSI DENGAN STREPTOZOTOCIN Dhirgo Adji
Jurnal Sain Veteriner Vol 26, No 2 (2008): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2586.578 KB) | DOI: 10.22146/jsv.322

Abstract

Diabetes mellitus is a major source of morbidity in developed countries. The morbidity and mortality areincreased in patients with diabetes mellitus compared with the general population. Relationship between highsugar or fat consumption with the increasing of glucose or blood cholesterol concentration has been wellunderstood, but the mechanism of diabetes mellitus as a result of streptozotocin injection and its relation withglucose and cholesterol concentration still need to be investigated. So far, many research mention thatstreptozotocin causes only the damage of a cell of pancreas which that directly relates to metabolism of bloodsugar. The purpose of the research is to investigate the mechanism of pancreas damage in its relation with lipidperoxidation. Sixteen male Wistar rats, 200 grams of bodyweight were used as animal models. Rats weredivided into two groups of eight animals each. Group I was not injected with streptozotocin, while Group II isinjected intraperitoneally with streptozotocin 50 mg/kg BW.Three days after the injection, blood samples weretaken intracardially for Malondialdehida (MDA), glucose and total cholesterol analysis. A one-way analysis ofvariance showed that there were significance differences between Group I and II (P
HUBUNGAN KUALITAS SUSU DENGAN KERAGAMAN GENETIK DAN PREVALENSI MASTITIS SUBKLINIS DI DAERAH JALUR SUSU MALANG SAMPAI PASURUAN Djoko Winarso
Jurnal Sain Veteriner Vol 26, No 2 (2008): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3966.177 KB) | DOI: 10.22146/jsv.323

Abstract

The objectives of this study were to identify: (1) the differences of milk quality at the farmers' levels, postcollection, Village Cooperative Unit (VCU) and Milk Processing Industry (MPI), (2) the relation between milkprotein to genetic variety of kappa casein, (3) the relation between the milk bacteria and the subclinical mastitisprevalence. Completely Randomyzed Design followed by Least Significant difference test was used for analyzingthe milk quality. The alele and genotype frequency were analyzed using Hardy- Weiberg method, while theprevalence of subclinical mastitis was assessed using Chi-Square. The result of the research showed that thepercentage of chemical milk composition, based on the milk quality standard (Grade), from the level offarmerto post collection had the biggest decrease of milk quality, especially for fat content (59.32%), TSL (58.33%)and TPC (22.96%). Statistically especially there was no significance influence on the protein content of milk bythe frequency of alele A, alele B, genotype frequency AA and BB. Genotype frequency AB, showed significanceinfluence (P
PENGARUH PANHISTEREKTOMI TERHADAP RETENSI KALSIUM DAN FOSFOR TIKUS SPRAGUE DAWLEY YANG DIBERI PAKAN KEDELAI SELAMA EMPAT MINGGU Hartiningsih .; Agus Irwanto; Rosana Anita Sari; Devita Anggraini
Jurnal Sain Veteriner Vol 26, No 2 (2008): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4123.26 KB) | DOI: 10.22146/jsv.324

Abstract

The objective ofthe current research was to study the effect of panhisterectomy on calcium and phosphorretention in Sprague Dawley rats fed with soybean for four weeks. Ten female Sprague Dawley rats, sixweeks of age were randomly divided into two groups (control and panhisterectomized group) of five each. Ateight weeks of age, the rats ofpanhisterectomized group were panhisterectomized. At 12 weeks of age, theywere placed into individual metabolic cages for balance study. Every morning, from day four to eight of thebalance study, the left-over feed was collected for calcium and phosphor analyses. Urine and fecal sampleswere also collected at the same time. The results of the research showed that calcium and phosphor retentionswere significantly reduced (P
KORELASI PANJANG EKOR DAN PANJANG TUBUH TERHADAP JENIS KELAMIN ULAR SANCA BATIK (Python reticulatus Slamet Raharjo; Dionisius M. .; Guntari Titik Mulayni; Soedarmanto Indarjulianto; Ida Tjahajati
Jurnal Sain Veteriner Vol 26, No 2 (2008): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3180.481 KB) | DOI: 10.22146/jsv.325

Abstract

The unique of Python reticulatus has been studied, however the correlation of the length and body weighthaven't studied yet. This objective ofthe research was to study the correlation of the tail and the body length ofPython reticulatus. Ten Python reticulatus, 2 weeks of old, were used in this research. The snakes wereplaced in individually cage at room temperature, consumed I to 2 mice and white mouse weekly and water adlibitum. The tail and body length of the snakes were measured at the beginning and the end of this research.The datas were analyzed using correlation regretion method. The result of this experiment showed that therewas a significan correlationbetweenthe lengthand body weight (R=O,85).The result of this research showedthat there is a significan correlation with R=O,98 in male snake, and R=O,71 in female snake. It's means thatthere is correlation between tail and body length on Python reticulates sexing.Key words: Python reticulatus, tail and body lengt, sexing
STATUS VAKSINASI RABIES PADA ANJING DI KOTA MAKASSAR RABIES VACCINATION STATUS OF DOGS IN MAKASSAR Sri Utami; Bambang Sumiarto; Heru Susetya
Jurnal Sain Veteriner Vol 26, No 2 (2008): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3584.959 KB) | DOI: 10.22146/jsv.326

Abstract

Telah dilakukan suatu analisis cakupan vaksinasi rabies pada anjing di kota Makassar. Tujuan penelitian iniadalah untuk mengetahui cakupan vaksinasi anjing bertuan, serta asosiasi antara status vaksinasi dan faktorfaktormanajemen pemeliharaan anjing. Sebanyak 246 sampel anjing dipilih untuk mengetahui status vaksinasirabies. Analisis status vaksinasi dan faktor pemeliharaan anjing dilakukan secara deskriptif. Uji Chi square (c2)dan odds ratio (OR) digunakan mengetahui asosiasi antara faktor-faktor yang diteliti dan status vaksinasi.Hasil penelitian menunjukkan bahwa cakupan vaksinasi pada anjing bertuan di kota Makassar sangat rendah(21 %). Rendahnya cakupan vaksinasi tersebut diperkirakan berhubungan dengan cara pemeliharaan (P=0,002;OR=4,3), tujuan pemeliharaan (P=0,009; OR=2,3), pengetahuan pemilik tentang rabies (P=0,001; OR=3,0),penghasilan di atas Rp 2.000.000,00 (p=0,000; OR=5,0), dan lokasi pemeliharaan urban area (P=O,OOOI; OR=4, 1).Kata kunci: Cakupanvaksinasi, rabies, faktor risiko
PERBANDINGAN TITER ANTIBODI AYAM BROILER YANG DIVAKSIN PADA UMUR 7 DAN 14 HARI MENGGUNAKAN VAKSIN AVIAN INFLUENZA HETEROLOG SUBTIPE H5N2 Ukon Susetyo; Michael Haryadi Wibowo
Jurnal Sain Veteriner Vol 26, No 2 (2008): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5594.985 KB) | DOI: 10.22146/jsv.327

Abstract

The aim of this study was to compare the titer antibody respons induced by different vaccination programusing heterologous avian influenza H5N2 subtype at day seven and 14 in broiler chicken. A number of 120broiler chickens was divided into three groups, each consisted of 40 birds and kept since day old chicken instandard maintenance facilities. Group one as the treatment group was vaccinated at 7 days of age usingheterologous vaccine H5N2 subtype. The second group was vaccinated at 14 days of age using the samevaccine. The control group, received no vaccination. A number of24 birds in each group were randomly chosenand bled via brachialis vein at day 14, 21 and 28 post vaccination, respectively. The antibody titer in the serawas measured using hemaglutination inhibition (HI) test with homologous antigen H5N2 subtype. Data wereanalysed using a split-plot design of analysis of variance of 0,05, significance level. The titer induced by vaccinationat day-7 were 1,54; 15,92 and 6,58 HI unit, respectively. Meanwhile in the second group the titer obtained were1,29; 7,38 and 15 HI unit respectively. Antibody of the control group was negativ. Further analysis indicated thatthere was significance different antibody titer induced by vaccination between treatment groups, but whencompared between group of treatment, the result showed po significance different.Keys words: avian influenza. broiler chicken, heterologous vaccine, and antibody titer
MONITORING PENGGUNAAN AMOKSISILIN, AMPISILIN DAN KLORAMFENIKOL PADA KUCING DI RUMAH SAKIT HEWAN-FAKULTAS KEDOKTERAN HEWANUNIVERSITAS GADJAH MADA TAHUN 2005-2007 Yanuartono .
Jurnal Sain Veteriner Vol 26, No 2 (2008): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3232.772 KB) | DOI: 10.22146/jsv.328

Abstract

The use amoxicillin, ampicillin and chloramphenicol for cats in animal hospital, Faculty of Veterinary Medicine,Gadjah Mada University, was studied during 2005 - 2007. The use of amoxcicillin in cats was formed to be8,06%,11,78%, and 18,97% in 2005, 2006, and 2007, respectively. The values for amoxcicillin were 30,99%,24,95%, and 12,37%, while for chloramphenicol were 3,92%, 4,59%, and 8,86%. Data showed that the use ofamoxicillin and chloramphenicol were increased, however, the use of ampicillin was decreased year by year.The result of study showed that use of antibiotic in animal hospital, Faculty of Veterinary Medicine, GadjahMada University did not very very much. Besides amoxicillin, ampicillin and chloramphenicol, oxytetracyclin,enrotloxacin and cyprotloxacin were used in small percentage.Key words: amoxicillin, ampicillin, chloramphenicol, cats
nalisis Keragaman Genetik Streptococcus agalactiae Penyebab Mastitis Subklinis Pada Sapi Perah Menggunakan pulsed field gel electrophoresis (PFGE) = Genetic Analysis of Streptococcus agalactiae Caused Subclinical ... Agnesia Endang Trihastuti
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2546.757 KB) | DOI: 10.22146/jsv.338

Abstract

Streptococcus agalactiae atau Streptokokus grup B (SGB) adalah salah satu bakteri utama penyebab mastitis subklinis pada sapi perch dan merupakan parasit obligat pada ambing. Karakterisasi S.agalactiae biasanya dilakukan secara konvensional menggunakan metode serotyping. Meslci metode ini sering digunakan namun masih mempunyai kelemahan apalagi masih adanya isolat S.agalactiae yang belum dapat dimasukkan ke dalam serotipe yang ada (nontypeable/NT), oleh karena itu pendekatan bare dengan metode genotyping digunakan. Tujuan dari penelitian ini adalah melihat profil DNA menggunakan PFGE dan kekerabatan isolat dari masing-masing serotipe maupun masing-masing daerah. Penentuan serotipe S. agalactiae dilakukan dengan metode serotyping menggunakan antiserum spesifik terhadap 9 serotipe S. agalactiae dengan uji imunodifusi/ agar gel presipitasi (AGP). Analisa genotipe S. agalactiae dilakukan menggunakan macro restriction fragment length polymorphism (MLFP)/ metode schizotyping menggunakan Pulsed-field gel electrophoresis (PFGE). Hasil dari penelitian ini adalah genotipe dari S. agalactaie dengan enzim restriksi Smal dihasilkan potongan-potongan pita Deoxyribo Nucleic Acid (DNA) yang jelas. Ada 3 isolat S. agalactiae yang tidak dapat dipotong oleh ensim restriksi Smal. Analisa DNA genom dari 21 isolat S.agalactiae dihasilakn 15 profil DNA.
ntracellular Localization of HBV Capsid in Hepatocyte Line After Transfected by The Entire HBV Genome = Lokalisasi Intraseluler Kapsid HBV Pada Sel Line Hepatosit Setelah Ditransfeksi Dengan Genom Utuh HBV. Aris Haryanto; Michael Kann
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2750.247 KB) | DOI: 10.22146/jsv.339

Abstract

HBV replicates within the nucleus of hepatocyte using cellular transport machinery for the import of their genomes into the nucleus. Genome of HBV has to transported through the cytoplasm towards the nuclear pore complex (NPC) followed by subsequent passage through the pore. HBV capsid is involved in a number of important functions in the replication cycle of HBV. It can be detected in the nucleus, cytoplasm or both within infected hepatocytes. Nuclear localization of HBV capsid protein, which is karyophilic, depends on the cell cycle. The objective of the present study was to analyzes the intracellular localization of HBV capsid protein after transfected by entire HBV genome into hepatocyte cell lines (HuH-7) and to determine the predominantly localization of the capsid into cell compartment. In this work we analysed the intracellular localization of the HBV capsid in human hepatocyte cell lines liuH-7 by transfection using entire HBV genome and transient expression. The transfected cells were fixed and an indirect immune staining against the HBV capsid was performed to detect the capsid. To verify the location within the cell, an additional co-staining against the nuclear pore complexes was performed. The Intracellular localization of the HBV capsid and NPC were analyzed by a confocal laser scan microscope. The observed of localizations into the transfected cells were classified to be predominantly as nuclear localization, cytoplasmic localization or distributed within both of these compartments. Result of this study indicated that Staining of HBV capsid found predominantly within the nucleus (71%). Less frequently, the HBV capsid localized within the cytoplasm (26%). Only in a minority of cases, the capsids were localized within cytoplasm and nucleus (3%). This low frequency indicate that the capsids were not diffusing within the cells being in accordance to the in vivo situation in which the nuclear membrane was impermeable for the capsid.
Kajian Kasus-kontrol Avian Influenza Pada Unggas di Jawa Timur, Jawa Tengah dan Daerah Istimewa Yogyakarta= A Case-control Study on Avian Influenza in Poultry in East Java, Central Java and Yogyakarta Special Province. Dyah Ayu Widiasih; Heru Susetyo; Bambang Sumiarto; Charles Rangga Tabbu; Setyawan Budiharta
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1791.726 KB) | DOI: 10.22146/jsv.341

Abstract

Kajian kasus-kontrol yang dirancang untuk menyidik kejadian avian influenza (Al) dan mencari hubungannya dengan faktor resiko penyakit, telah dilakukan terhadap 218 dusun di Jawa Timur, Jawa Tengah dan Daerah Istimewa Yogyakarta. Sebagai kasus (109 dusun) adalah dusun yang pernah dilaporkan atau sedang mengalami kasus AI, dan kontrol (109 dusun), adalah dusun yang dilaporkan belum pernah mengalami, tetapi dekat dengan dusun kasus. Kuesioner digunakan untuk menjaring variabel yang diperkirakan berasosiasi dengan kejadian AI. Data yang diperoleh dianalisis dengan Chi Square (x2) dan odds ratio (OR). Hasil kajian menunjukkan bahwa faktor adanya hewan pengerat (OR = 1,90), faktor adanya burung liar (OR = 24,00), faktor pekerja pulang sehabis kerja (OR = 2,65), dan faktor sektor III (OR = 1,79) mempunyai asosiasi karat dengan kejadian AI di suatu dusun, sedangkan beberapa faktor biosekuriti berasosiasi lemah (OR = 1,0 – 1,5) terhadap kejadian Al.

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