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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 824 Documents
 61   62   63   64   65 
Farmers' Characteristics Associations with the Knowledge for Sheep Estrus Detection in Batur Village, Banjarnegara, Indonesia Alek Ibrahim; Wayan Tunas Artama; Rini Widayanti; Bayu Andri Atmoko; I Gede Suparta Budisatria; Dyah Ayu Larasati
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.68462

Abstract

The accuracy of the estrus detection was important in sheep breeding management. This study aimed to determine the correlation between the farmers’ characteristics and the knowledge on estrus detection of their sheep. The data were collected by interviewing 33 farmers, who have raised sheep with a breeding system for at least two years of experience in Batur Village, Banjarnegara, Indonesia. The farmers’ knowledge for estrus detection was divided into three-level, namely low, medium, and high levels. The results showed that the farmers’ knowledge for estrus detection was 51.5%, 27.3%, and 21.2% on the low, medium, and high levels, respectively. There was a significant relationship between formal education, informal education, number of sheep, the sheepfold position, stay duration in the sheepfold, and the ram ownership for mating toward knowledge of estrus detection level. It can be concluded that there is a relationship between farmer characteristics in Batur Village and the ability to detect estrus in ewes, which is dominated at low levels, so it is necessary to increase farmers' knowledge regarding estrus detection.
Komparasi Lima Jenis Primer Polymerase Chain Reaction Untuk Mengidentifikasi Kelamin Burung Famili Columbidae Yang Akurat Fauziah Fitriana; Riza Resita; Yuda Disastra; Gioknio Happy Alfatik; Clara Ajeng Artdita; Aris Haryanto; Fatkhanuddin Aziz
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.68787

Abstract

Penentuan jenis kelamin pada beberapa spesies burung cukup sulit dilakukan dikarenakan jantan dan betina memiliki ciri morfologi yang sama (monomorfik), salah satunya famili Columbidae. Teknik penentuan jenis kelamin burung secara molekuler yang populer adalah metode Polymerase Chain Reaction (PCR) dengan gen target Chromo Helicase DNA-binding (CHD), namun keberhasilan amplifikasi gen target pada PCR tersebut dipengaruhi salah satunya kesesuaian DNA template dengan primer yang digunakan. Penelitian ini bertujuan untuk mengevaluasi 5 jenis primer PCR P2/P8, 2550F/2718R, CHD1F/CHD1R, 1237L/1272H dan CHD1LF/CHD1LR untuk menentukan jenis kelamin famili Columbidae. Penelitian ini dilakukan dengan menguji 5 jenis primer tersebut di atas pada sampel DNA tiap pasang jantan dan betina dari burung merpati, balam jambi, punai, derkuku, dan perkutut. Hasil penelitian diketahui primer CHD1LF/CHD1LR menunjukkan hasil terbaik dan direkomendasikan untuk menentukan jenis kelamin famili Columbidae.
POLA PEMELIHARAAN BURUNG WALET PADA PULAU-PULAU UTAMA PENGHASIL SARANG BURUNG WALET DI INDONESIA Dede Sri Wahyuni; Hadri Latif; Mirnawati B Sudarwanto; Chaerul Basri
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69112

Abstract

Burung walet di Indonesia umumnya dibudidayakan pada rumah burung walet (RBW) secara tradisional dengan pola pemeliharaan tertentu. Pola pemeliharaan menjadi salah satu faktor pendukung bagi burung walet untuk memproduksi sarang burung walet (SBW) dengan kualitas baik secara berkelanjutan. Penelitian ini bertujuan untuk menganalisis pola pemeliharaan burung walet di RBW di pulau-pulau utama penghasil SBW di Indonesia. Suatu survei terhadap total 44 RBW di pulau Jawa, Sumatera, Sulawesi, dan Kalimantan telah dilakukan untuk mengetahui pola pemeliharaan di masing-masing pulau tersebut. Data dikumpulkan melalui wawancara secara langsung dengan menggunakan kuesioner terstruktur. Pertanyaan dalam kuesioner terdiri atas karakteristik bangunan, kebersihan, sumber makanan dan udara, dan lingkungan RBW. Hasil penelitian menunjukkan bahwa bangunan RBW umumnya bertingkat, dengan atap beton, dinding bata semen, lantai plester semen, dan sirip kayu. Rumah burung walet dibersihkan dibersihkan dengan cara digores/disapu dalam waktu kurang dari dua bulan. Kotoran burung walet umumnya digunakan untuk kebutuhan sendiri. Rumah burung walet umumnya tidak menyediakan bahan untuk menarik serangga atau pakan tambahan. Pakan burung walet umumnyaHymenoptera , dan sumber airnya adalah kolam di dalam gedung RBW. Lingkungan RBW merupakan kawasan pemukiman dan dekat dengan jalan raya. Pembinaan dan pemantauan terhadap pola pemeliharaan burung walet perlu terus dilakukan untuk mendapatkan SBW yang berkualitas baik. 
Uji In Vitro Efektivitas Ekstrak Biji Jintan Hitam (Nigella Sativa L.) terhadap Pertumbuhan Microsporum gypseum Penyebab Dermatitis pada Anjing Pasha Glabella; Salma Ramandhanti Putri; Ery Haryani; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69251

Abstract

Biji jintan hitam (Nigella sativa L.) mengandung senyawa aktif seperti thymoquinone, carvacrol, dan thymol yang berperan sebagai antijamur. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Microsporum gypseum yang merupakan salah satu agen penyebab dermatitis pada anjing. Kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Mircrosporum gypseum diuji dengan metode difusi agar dengan teknik sumuran (Agar Well Diffusion). Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak biji jintan hitam terhadap pertumbuhan jamur Microsporum gypseum yaitu 10%, 25%, 50%, 75%, 100%, flukonazol digunakan sebagai kontrol positif dan phospat buffer saline (PBS) kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak biji jintan hitam dengan konsentrasi mulai dari 25%, 50%, 75% dan 100% dapat menghambat pertumbuhan jamur dengan rerata lebar zona hambat secara berurutan ± 3,67 mm., ± 2,84 mm., ± 3,67 mm., dan ± 4,00 mm. Ekstrak biji jintan hitam mampu menghambat pertumbuhan Microsporum gypseum.  Perlu penelitian lebih lanjut untuk mendapatkan hasil optimal sehingga berpeluang sebagai anti fungal pada penyakit dermatitis pada anjing.
In Vitro Anthelmintic Areca catechu Crude Aqueous Extract Against Haemonchus contortus in The Sheep Annisa Putri Cahyani; Budi Purwo Widiarso; Supriyanto -; Wida Wahidah Mubarokah
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70399

Abstract

Haemonchosis is a gastrointestinal parasitic disease in sheep caused by Haemonchus contortus. The biggest economic losses due to this disease are mortality, decreased production, stunted growth, and low body weight. The existence of resistance to synthetic anthelmintics causes the development of research on alternative treatments to herbal anthelmintics to be a strategic step. This study aims to analyze the effect of Areca catechu on Haemonchus contortus in vitro so that the Lethal Concentration 50 (LC 50) can be determined. This study was divided into 9 groups. Group I was treated with 2.5% A. catechu crude aqueous extract; group II was treated with 5% of A. catechu crude aqueous extract; group III was treated with 7.5% A. catechu crude aqueous extract; group IV was treated with 10% of A. catechu crude aqueous extract; group V was treated with 12.5% A. catechu crude aqueous extract; group VI was treated with 15% A. catechu crude aqueous extract; Group VII was treated with 17.5% A. catechu crude aqueous extract, group VIII was a negative control (0.9% NaCl) and group IX was a positive control (Albendazole). The mortality of H. contortus was recorded every hour until the worm mortality was 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test of Areca catechu crude aqueous extract against H.contortus it can cause mortality in worms with an Lethal Concentration 50 (LC 50) calculation result of 11.11%.
The Vermicidal Activity of Water Extract Two Types of Ethnopharmacology Against Haemonchus contortus in Kacang Goat (Capra hircus) In-vitro I Gusti Komang Oka Wirawan; Suryawati -; Theresia Nur Indah Koni; Redempta Wea
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70779

Abstract

This research aims at determining the differences in the vermicidal activity between EADMAN and EADMDV in-vitro based on concentration and treatment time. It encompassed three groups of concentrations of EADMAN and EADMDV solution (2.5%, 3.5%, 4.5% from 0.2 g/mL each extract solution), a group of negative control (aqua pro injection), and a group of positive control (Oxfendazole 0.055%). The EADMAN and EADMDV was diluted in 1.5 mL aqua pro injection, then placed on petri dish with a concentration of 2.5%, 3.5%, 4.5% of 0.2 g/mL extract solution. Eight worms placed on each petri dish afterwards. The effectivity of EADMAN and EADMDV was determined based on the percentage of dead worms. The dead and live worms was counted from each immerse group for 1, 3, 5, 7 hours with four replications for each treatment. The vermicidal activity was indicated by touching the worms using a spatula; if it did not react, it was put in warm water. If the worms did not move, it was declared as the dead one, otherwise it was paralysed. Data of effective concentration from both extracts against vermicidal activity was analyzed using a two-way Anova, followed by Tukey test (P>0.05). The result showed the vermicidal activity of 2,5% EADMDV was significantly greater than 4.5% EADMDV, the negative control, and EADMAN at all concentrations. Meanwhile, its difference to 3.5% EADMDV and positive controls revealed insignificant results statistically. Therefore, 2.5% concentration of EADMDV with 7 hours of immersions gave the most effective vermicidal activity against Haemonchus contortus.
Early Detection of Contamination Vibrio parahaemolyticus and Escherichia coli in Fisheries Product Using Multiplex Polymerase Chain Reaction Nur Hasanah; Putu Eka Sudaryatma; Imanuddin Razaq; Ni Nyoman Eriawati; Wahyu Andy Nugraha; Hidayati Kumalasari; Ni Putu Arya Shintya Anggraeni; Ida Ayu Mirah Meliana Dewi
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.73314

Abstract

The fisheries sector provided a significant contribution to the Indonesian economy by increasing export activities in every year. The exported fisheries product are categorized of live fish, frozen fish, preservation products from various types of fish, crustaceans, and molluscs. The contamination of pathogenic bacteria Vibrio parahaemolyticus and Escherichia coli causing healthy problems originating from the fishery sector (sea-food borne disease). These two bacteria contaminated fisheries product is due to mishandling and storaging in the processing, which causes acute diarrhea, gastrointestinal infections and fever. The multiplex polymerase chain reaction (mPCR) method was developed to increase the efficiency of time, effort and accuracy of the bacterial contamination testing process. The mPCR method begins with the optimization of the two bacterial gene targets, sensitivity test, specificity test and then applied to samples of fishery products. The mPCR method is carried out in two mechanisms, namely “one-run” conducted from bacterial colonies isolated on agar media and “one-tube” which is applied directly from fishery products. The results of the development of the mPCR method on V. parahaemolyticus and E. coli resulted in sensitivity at concentrations of DNA 5.6 pg/ml and DNA 5.5 pg/ml, respectively. One-tube mPCR application obtained 7 positive colonies of V. parahaemolyticus and 38 positive colonies of E. coli. Meanwhile, one-tube mPCR which was applied directly from shrimp samples could identify the two bacteria.
Infection of Infectious Spleen and Kidney Necrosis Virus and Viral Nervouse Necrosis in Seawater Fish Identifed Using Multiplex Polymerase Chain Reaction Hidayati Kumalasari; Putu Eka Sudaryatma; Artanti Tri Lestari; Wahyu Nurlita; Wahyu Andy Nugraha; Nur Hasanah; Ida Ayu Mirah Meliana Dewi; Ni Putu Arya Shintya Anggraeni
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.73599

Abstract

Indonesia become a maritime country with large of ocean area and beach line with marine species diversity reaches 37% of the world’s fish. This makes Indonesia have aquaculture potential that supported by an appropriate climate. However, the challenge of viral diseases caused by Infectious Spleen and Kidney Necrosis Virus (ISKNV) and Viral Nervous Necrosis (VNN) would hamper marine aquaculture and causing huge economic losses. Therefore, it is necessary to carry out early detection methods that are efficient, fast, precise and accurate to identifying these viral disease. Here we developed a multiplex-PCR (mPCR) a method that can detect simultaneously of the ISKNV and VNN. The results of this method showed high sensitivity and specificity by using MCP and CP gene target primers to detect ISKNV and VNN, respectively. From a total of 353 samples of seawater fish examined by mPCR, positive results following of single infection of ISKNV and VNN were 14 and 2, respectively. We also found that 18 grouper fish was co-infection with these viruses. From these results, it shows that the mPCR method developed has efficiency, faster and high accuracy. This founding was possible to be applied in laboratory testing or early detection system in the field for ISKNV and VNN. 
Fixation of Sacroiliac Fraktur-Luxation Using Plate 2.0 with screw 2.0 mm cortical self-tapping and 2.7 mm cortical non self-tapping Erwin .; Rusli .; Amiruddin .; Teuku Fadrial Karmil; Muhammad Fauzih Asjikin; Afif Yuda Kusuma
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53112

Abstract

The Mixed Shih-Tzu dog, 3 years old and 4.3 kg body weight, had clinical signs of unable to stand, both rear limbs suffer from diplegic lameness so that they are only able to rely on both forelimbs. The owner said that her dog is traumatized by a four-wheeled vehicle. The results of blood profile before surgery showed physiological, only an increase in leukocyte cells 18.83 x103/µL. The radiographic examination with a ventral-dorsal position shows oblique fractures in the pelvic dexter and dislocation of the pelvic sacroiliac sinister bone. The method of handling used was open reduction internal fixation (ORIF) using a 2.0 veterinary cuttable plate (VCP) bone plate with a 2.0 mm cortical self-tapping screw for pelvic fractures and 2.7 mm cortical non-self-tapping for sacroiliac dislocations. The dog walked and showed a good progress in one week after ORIF, based on clinical and post-operative radiographic examinations, sacroiliac fracture-dislocation fixation using VCP with 2.0 mm self-tapping screw cortical for pelvic fracture and 2.7 mm non self-tapping for sacroiliac dislocation provides excellent stabilization and no visible complications.
Combination of Wild ginger, Honey, and Probiotics as Growth Prometer Candidates : An in Vitro Study Marlin Cindy Claudya Malelak; Agnesia Endang Tri Hastuti Wahyuni; Agustina Dwi Wijayanti; Vinsa Cantya Prakasita
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53256

Abstract

Growth Promoter Antibiotics are used to prevent disease and promote growth and production in poultry. Repeated admnistration of feed can have a microorganic resistance effect, accumulation of antibiotic residues in animal and environmental products and imbalance of normal microflora in the intestine. The antibacterial and carbohydrate content of some natural ingredients can be potential as a replacement candidate for AGP. This study aims to determine the role of a combination of wild ginger, honey, and probiotics (Bacillus subtilis and Lactobacillus acidophilus) as AGP candiate in Vitro. The antibacterial activity of the combination of wild ginger and honey against pathogens (E. coli) and their use against probiotics was tested by disk diffusion method, while the calculation of optical density values to determine the minimum inhibitory concentration and minimum bactericidal concentration was carried out on E. coli. The ability of inhibition of probiotics against pathogens is also done by the disk diffusion method. The disk diffusion test results showed the best combination was extract of 25% wild ginger aquades + 100% Lombok honey with inhibition zone diameter (8,53 ±, 03). Optical density values indicate this combination is able to inhibit and kill E. coli (DO 0,00 ±, 002) and support B. subtilis (DO 0,18 ±, 002) and L. acidophilus (DO 0.25 ±, 005) significantly better than positive control. MIC value of wild ginger aquades extract and honey combination against E. coli is wild ginger aquades extract 3.13% + Lombok honey 25%, while MBC value is wild ginger aquades extract 6,25% + Lombok honey 25%. The combination of B. subtilis and L. acidophilus showed the largest inhibitory zone diameter against E. coli (7,30 ±, 02 mm) compared to individual colonies. The combination of wild ginger and honey, in addition to inhibiting also able to kill pathogens and support the growth of probiotics, so this formula can be used as one of the replacement candidates for AGP.  

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