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Buletin Peternakan
ISSN : 01264400     EISSN : 2407876X     DOI : https://doi.org/10.21059/buletinpeternak
Core Subject : Health, Education,
Bulletin of Animal Science is published every four months. The Annual subscription rate is Rp. 150.000,-/year. Bulletin receives original papers in animal science and technology which are not published at any other journals.
Arjuna Subject : -
Articles 1,046 Documents
Small Intestine Characteristics and Nutrient Retention in Broiler Chickens Submitted to Different Protein Regimes and Betaine Supplementation Adi Ratriyanto; Sunarto Sunarto
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i1.51142

Abstract

This study was designed to determine the intestinal characteristics and nutrient retention of broiler chickens subjected to different protein regimes supplemented with betaine. Four experimental diets were formulated, consisting of two basal diets containing 20% and 23% crude protein (CP), both with and without betaine supplementation at the level of 0.14%. The diets were applied to 180 broiler chickens that were randomly allotted to 2×2 factorial arrangement with five replicates of nine chickens each. The diet with 20.0% CP generated better small intestine characteristics than the diet with 23.0% CP as indicated by the longer ileum and total small intestine length (P<0.05). This improvement was associated with lessened CP excretion and improved dry matter (DM) and CP retention (P<0.05) in the birds fed 20% CP. Furthermore, dietary betaine supplementation enhanced the ileum, total small intestine length, villus height, and villus-to-crypt ratio (P<0.05), indicating a greater surface area for nutrient absorption. This enhancement was reflected in the reduction of DM and CP excretion and improvement in DM and CP retention in the betaine supplemented group (P<0.05). Therefore, we conclude that feeding a diet of 20% CP with betaine supplementation improved the small intestine characteristics and nutrient retention of broiler chickens.
Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method Freshinta Jellia Wibisono; Bambang Sumiarto; Tri Untari; Mustofa Helmi Effendi; Dian Ayu Permatasari; Adiana Mutamsari Witaningrum
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.51347

Abstract

This study aimed to determine the resistance profile and the nature of multidrug resistance in Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E.coli) against several classes of antibiotics. Positive isolates of ESBL-producing E.coli were tested for antibiotic sensitivity using the VITEK® 2 compact method which then analyzed automatically. The results showed an antibiotic resistance profile against ESBL-producing E.coli showed the highest level of antibiotics in beta lactam, amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone at 100%. Subsequent results found a relatively high level of resistance in the antibiotics aztreonam (86.36%), trimethoprim/sulfamethoxazole (77.27%), gentamicin (72.73%), and ciprofloxacin (68.18%). Antibiotics from carbapenem groups such as ertapenem and memenem, and antibiotics from the aminoglycosides (amicasin) and tigecycline groups of tetracycline still showed a high sensitivity level of 100%. The most common resistance patterns found in ESBL-producing E.coli isolates are AM/AMP/KZ/CTX/CRO/ATM/GM/CIP as much as 22.73%, and AM/AMP/KZ/CTX/CRO/ATM/GM/CIP/SXT patterns of 18.2%. The results of multi-class antibiotic resistance showed that 86.36% had multidrug resistance. The highest multidrug resistance pattern in ESBL-producing E.coli occurred with a BL/AG/Q/SP pattern of 50%. Other patterns of multidrug resistance in ESBL-producing E.coli that can be found in this study are, the BL/AG/Q/SP pattern is 18.20%, the BL/AG/Q/SP pattern is 13.64%, and the BL/AG/Q pattern is 4.55%. The high profile of resistance and the nature of multidrug resistance in ESBL-producing E.coli has the potential to spread these resistant genes, thus risking the use of antibiotics as a public health therapy and animal health, therefore further evaluation and control are needed.
The Effect of Young Coconut Husk on the Quality of Goat Manure-Chicken Excreta Bioculture Dwi Ahmad Priyadi; Dyah Triasih; Sefri Ton; Ari Istanti
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.51434

Abstract

The negative impact of chemical fertilizers use is soil fertility declining. The situation occurs because of biological, physical, and chemical properties of the soil is decreased. Agricultural waste is a large commodity which utilization can still be optimized, for example by using as raw material for organic liquid fertilizer. Agricultural wastes that have good quality as fertilizer include goat manure, chicken excreta, and coconut husk. The utilization of agricultural waste as organic fertilizer is one way of creating sustainable agriculture. This study aims to test the quality of liquid fertilizer (bioculture) of goat manure and chicken excreta by adding various levels of coconut husk. Bioculture is made by anaerobic fermentation for 21 days. The parameters observed included levels of C-organic, N, P, and K bioculture, as well as germination tests to determine the presence of phytotoxins. The data were analyzed using one way ANOVA. The treatment of P5 (5% coconut husk) showed the highest levels of N and P, while the K content was not significantly different from the treatment of P4 (2.5% coconut husk). The germination test showed no phytotoxin activity in bioculture.
Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation Zaituni Udin; Masrizal Masrizal; Hendri Hendri; Syafri Nanda
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.52181

Abstract

This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1;  the storage duration of ovaries were 6, 9 and 12 h. Experiment -2;  the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P<0.05) as the effect of storage time. The average number of oocytes were 15.90±2.48; 17.10±1.38 and 13.60±3.00, in 6 h; 9 h and 12 h of storage time, respectively. The techniques of oocytes collection significantly (P<0.05) affect the quantity of oocytes but no significant (P>0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P<0.05) on immaturation rate of bovine. Conclusion of this study the storage time of ovaries was suitable in 9 h and the technique of oocytes collection was in slicing plus aspiration.
Effect of Turmeric and Garlic Inclusion to Sauropus androgynus-Bay Leaves Containing Diets on Performance, and Carcass Quality of Broilers Urip Santoso; Yosi Fenita; Kususiyah Kususiyah; Kiki Rusdi; Elsa Marta Savitri; Novriska Winni Simanjutak
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.52285

Abstract

This study aimed to examine the effect of turmeric or garlic supplementation to fermented Sauropus androgynus-bay leaves (FSBL) containing diet on performance, carcass quality and meat organoleptic status in broilers. This study used a completely randomized design. Two hundred 15-day-old female broilers were grouped into 5  groups with 4 replications as follows: Diet with 0.5% commercial feed additive (P0); 1.25% FSBL containing diet (P1); 1.25% FSBL containing diet plus 1 g turmeric powder (P2); 1.25% FSBL containing diet plus 2 g garlic (P3); 1.25% FSBL containing diet plus 1 g turmeric and 2 g garlic (P4). The results showed that the use  of turmeric or garlic to FSBL containing diet did not affect body weight gain, feed intake, feed conversion ratio, carcass weight, meat bone ratio, drip loss, and taste, odor, and texture of broiler meats (P>0.05) but affected cooking loss (P<0.05). In conclusion, the inclusion of garlic and/or turmeric to fermented Sauropus androgynus-bay leaves containing diet did not improve performance and carcass quality in broiler chickens. Any combination of medicinal plants could replace commercial feed additive.
Performances of Broiler Chickens Fed the Commercial Diets Partially Substituted with Feeds Containing Fermented and Non Fermented Leubim Fish Meal (Canthidermis maculata) zulfan zulfan; Muhammad Aman Yaman; Allaily Allaily; Enten Juni Marlina
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.52732

Abstract

The purpose of present study was to evaluate the performance of broilers fed the commercial diet partially substituted with leubim fish (Canthidermis maculata) waste meal (LFWM) treated by fermentation and without fermentation. As many as 100 broiler chicks, MB 202 strain, unsex, were reared up to 5 weeks in this study. The research was set up into randomized block design (RBD) consisting of 5 treatments and 4 blocks with 5 birds each. Blocks were as replicate based on the body weights of the birds at the beginning of feeding experimental diets. The experimental diets were commercial diets partially replaced with LFWM administered by either fermentation or not with the level of 6 and 12% each, 6.5% and 13% yellow corn, and 0.5% topmix. The data was analyzed using an Analysis of Variance (AOV) and continued by Duncan’s Multiple Range Test (DMRT) only if significant effects were detected among the treatments. The result of recent study indicated that the inclusion of feeds containing LFWM treated by either or not fermented combined with yellow corn and topmix as partially substitution of commercial diets significantly increased (P<0.05) FBW, BWG, feed intake, and protein intake compared to those fed full commercial diet. However, FCR and PER were not significantly affected. DMRT indicated there were no significant diferences in broiler performances fed the feeds containing LFWM treated by fermentation vs without fermentation.
Study of Pasture Productivity for Semi-Intensive Cattle System during Dry Season in the South Konawe Regency, Southeast Sulawesi Ahmat Endang Two Sulfiar; Bayu Andri Atmoko; Budi Guntoro; I Gede Suparta Budisatria
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.52742

Abstract

This study was aimed to evaluate the characteristics and productivity of pasture for semi-intensive cattle system in South Konawe Regency during dry season. The study was conducted in August-September 2019 as dry season based on the weather condition and was determine by Department of Statistics Center of South Konawe Regency. A total of 55 selected smallholder farmers in two areas in South Konawe Regency, namely West Ranomento (30) and Konda (25) Districts. Data were obtained by interview to find out the farmers profile and pasture characteristics. Production was measured by forage sampling (1x1m) to calculate annual production. Samples were proximately analyzed to calculate dry matter production and the carrying capacity. Data were analyzed descriptively and statistically with an independent sample t-test. The botanical composition in both locations was highly dominated (>90%) by field grasses. The grassland ownership was not different between two areas. Grassland ownership at West Ranomento and Konda were 0.78±0.14 and 0.21±0.04 Ha/farmer, respectively. The pasture production between West Ranomento (2.27±0.10 ton/ha) and Konda District (1.76±0.05 ton/ha) was significantly different (p<0.05),. Pasture production and carrying capacity indicated that conditions in West Ranomento were significantly higher (p<0.05) compared to Konda District. The fresh forages production was 10.98±2.26 vs. 2.17±0.51 ton/year, forages that can be consumed was 3.30±0.67 vs. 3.30±0.67ton/year, dry matter production was 0.72±0.15 vs. 0.18±0.04 ton/year and carrying capacity was 0.22±0.04 vs. 0.05±0.01 AU/year respectively. It was concluded that the pasture productivity at the study area during dry season was very low based on productivity and carrying capacity.
Nutrient Quality and in Vitro Digestibility of Bio-Ammoniated Rice Straw Bales Cuk Tri Noviandi; Dibya Ratnopama; Ali Agus; Ristianto Utomo
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.53409

Abstract

This study was done to determine the effects of bale sizes of bio-ammoniated rice straw on its nutrient quality and in vitro digestibility. Rice straw were bio-ammoniated by adding 2 g urea and 1 g probiotic/kg DM. By following a completely randomized design, rice straw was baled in 3 different weights (15, 25, and 35 kg) with six replications for each treatment, and then stored for 3 weeks. In the end of the week 3, bales were opened, aired, and then sampled for proximate analysis (dry matter, organic matter, crude protein, and crude fiber) and digestibility by in vitro gas production method. Using analysis of variance method, the proximate data showed that greater the bales size (15, 25, and 35 kg) increased crude protein (7.59, 7.86, and 9.95%, respectively; P<0.05) and decreased crude fiber contents (24.1, 22.1, and 18.8%, respectively; P<0.05). By increasing the size of bales also increased a, b, and c fractions (-0.79, 0.26, and 0.82 mL/100 mg DM; 20.2, 18.2, and 17.6 mL/100 mg DM; 0.012, 0.014, and 0.019 mL/h, respectively; P<0.05) as well as gas production (11.4, 11.5, and 13.8 mL/100 mg DM, respectively; P<0.05). It can be concluded that packing bio-ammoniated rice straw in 35 kg bale is the most effective way in increasing nutrient quality and digestibility of rice straw.
Identification of Glucogenic Amino Acids Content in Gliricidia maculata as an Alternative Energy Source for High-Yielding Periparturient Dairy Cows Sulvia Dwi Astuti SW; Budi Prasetyo Widyobroto; Ali Agus; Lies Mira Yusiati
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.54103

Abstract

Gliricidia maculata which has long been used as a forage for animal feed may contain a variety of glucogenic amino acids variants. The objective of this study was to identify the glucogenic amino acids content in Gliricidia maculata as an alternative source of energy for high-yielding periparturient dairy cows. The samples were the edible portion of plants, harvested randomly at the optimal cutting age (80 days), from the feed plant collection garden of The Faculty of Animal Science, Universitas Gadjah Mada. Lyophilization was carried out by drying the samples at 55°C for 3 x 24 hours continouesly in a Sanyo Drying Oven MOV-112. Pulverization was done by the Foss Tecator CyclotecTM 1093 Sample Mill with 300 mesh (1 mm screen). The hydrolysis of amino acid was carried out using HCl solution while amino acid derivatization used O-phtalaldehyde (OPA) solution. Separation, determination, and quantification of amino acid were carried out by an analytical method in gradient elution using the Thermo ScientificTM DionexTM UltiMateTM 3000 UHPLC Systems with Rapid Separation Fluorescence Detector. Result showed there were at least fourteen kinds of amino acids identified from the samples, i.e.: aspartic acid, glutamic acid, serine, histidine, glycine, arginine, alanine, tyrosin, methionine, valine, phenylalanine, isoleucine, leucine and lysine. Gliricidia maculata contains 1349 ppm glucogenic amino acids, 412.7 ppm ketogenic amino acids and 444.7 ppm glucogenic and ketogenic amino acids. Moreover, there were three types of glucogenic amino acids with the highest concentration were serine (288.7 ppm), glutamic acid (245.5 ppm) and phenylalanine (197.1 ppm) respectively. Glucogenic amino acids can be used as an energy source for dairy cows through gluconeogenesis. This study suggests that Gliricidia maculata may use to supply the precursors of energy for high-yielding periparturient dairy cows to prevent ketosis.
Identification of Locus GH/Alui Polymorphisms of Kuantan and Pesisir Cattle Hidayati Hidayati; Robbana Saragih
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.54183

Abstract

Kuantan cattle is a local beef cattle of Riau Province that adaptive and breed well along the Kuantan river flow, found in Indragiri Hulu Regency and Kuantan Singingi Regency, have smaller morphometric performance compared to other local beef cattle and are relatively the same as Pesisir cattle. One of the genetic markers of growth determinant in beef cattle is the GH/AluI gene locus. Identification of the diversity of the GH/Alu I gene was carried out using the PCR-RFLP. Fifty four samples of Kuantan cattle consisting of 25 samples from Indragiri Hulu Regency and 29 samples from Kuantan Singingi Regency, as well as 25 samples of Pesisir cattle from BPTU-HPT Padang Mengatas Payakumbuh, West Sumatra, were used in this study. The oligonucleotida forward F-5’GCTGCTCCTGAGGGCCCTTC-3’ and reverse R-5’CATGACCCTCAGGTACGTCTCCG-3’ used in this study along 211 bp of GH Gene. The PCR mixture consisted of DNA templates 20 - 25 ng, 0.4 µL  primer forward dan primer reverse (10 ng primer forward and primer reverse), 25 µL dream tag green master mix and ddH2O up to a 50 µL. PCR machine conditions consisted of 940C pradenaturation for 5 minutes, 940C denaturation for 30 seconds, annealing 650C for 1 minute, 720C extension for 50 seconds and 720C final extension for 5 minutes. Identification of GH gene diversity using AluI at an incubation temperature of 370C for 3 hours. The results in this study were found monomorphic of GH/AluI genes in Kuantan and Pesisir cattle. The monomorphic in Kuantan cattle can be used as an indicator to assess the purity of Kuantan cattle in this areas. 

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