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INDONESIA
Jurnal Serambi Biologi
Published by Universitas Negeri Padang
ISSN : -     EISSN : 27222829     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biologi Perkembangan
Articles 187 Documents
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Effect of Different Feeding on Uric Acid Levels in Mice (Mus musculus L.) Diana, Okta; atifah, Yusni; Helendra
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v8i2.212

Abstract

Aim Uric acid is an acid that is formed from purine metabolism in the body. If uric acid levels in the blood exceed normal limits it is called hyperuricemia. Diet plays an important role in increasing or decreasing uric acid levels. Consuming foods high in purines can also increase uric acid levels. The purpose of this study was to determine the effect of different feeds on uric acid levels in mice. Methods This research is an experimental research. The research method used was a completely randomized design (CRD) with 3 treatments and 7 replications for each group. Results There is a difference in the average uric acid levels of mice in the K, P1, and P2 treatment groups. In the K treatment group, the average uric acid level of mice was 3,2 mg/dl. In the P1 treatment group, the average uric acid level of mice was 7,3 mg/dl. In the P2 treatment group, the average uric acid level of mice was 4,2 mg/dl. Main conclusions Differences in mice uric acid levels are influenced by diet, and consumption of foods high in purines. The highest average uric acid levels in mice were in the P1 treatment group of 7,3 mg/dl.
Cadangan Karbon di atas Permukaan Tanah pada Kawasan Cagar Alam Maninjau Blok Rehabilitasi Silayang-Malabur, Kabupaten Agam Novia Sherina A. Lubis; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The Maninjau Nature Reserve is a conservation forest area located in the Agam district and Padang Pariaman district. In this case the Silayang-Malabur block is included in the rehabilitation block where this block has been damaged both naturally and due to human actions such as illegal logging, conversion of forests to fields and so on which has an impact on reducing carbon stocks. Therefore, it is necessary to do research in calculating carbon stocks to determine carbon stocks. This research was carried out in the Maninjau Nature Reserve area, Silayang-Malabur Rehabilitation Block, Agam Regency, which was conducted from December 2022 - January 2023. This type of research was descriptive research by collecting data on the level of trees, poles, saplings, and seedlings. Data collection used purposive random sampling which was divided into 2 research areas, namely natural forest and open/damaged forest. The sample plots used were a single plot of 3 plots randomly distributed in each study area. Biomass estimation using allometric equations. Based on the results of research on carbon stocks stored above ground level in the Maninjau Nature Reserve area of the Silayang-Malabur Rehabilitation Block, Agam Regency of 148,415 ton C/ha in natural forest areas and 34,397 ton C/ha in open/degraded forest area with a total carbon stock of 182,812 ton C/ha.
Struktur dan Status Regenerasi Alami di Hutan Konservasi Cagar Alam Maninjau Malabur Kabupaten Agam Nurul Fadhila Ilahi; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Data is needed regarding the structure and status of natural regeneration in the Maninjau Nature Reserve Conservation Forest which will be carried out from December 2022 - January 2023. The tools and materials used are labels, raffia rope, plastic bags, stationery, hygrometer, thermometer, lux meter, stake , meter, camera and GPS, carried out in 2 different places, namely natural areas and open/damaged areas. The sample plots used were nested square plots measuring 20x20m, ie 2x2m for the seedling phase, 5x5m for the sapling phase, 10x10m for the pole phase, and 20x20m for the tree phase. In each area 3 plots were placed randomly. Data is processed to obtain vegetation data and analysis of regeneration status. Plants found in natural areas at the seedling level are 12 species, 8 species for saplings, 4 species for poles and 11 species for trees, in open areas for seedlings 10 species, 5 species for saplings, 3 species for poles and 5 species for trees. Overall regeneration status can be said to be good because 7 species (35%) regenerate well, 2 species (10%) have sufficient regeneration, 6 species (30%) have low regeneration and 5 species (25%). In open/damaged areas it has a low regeneration density.
Isolasi Cendawan Endofit Pelarut Fosfat dari Akar Tumbuhan Pakis Simpei (Cibotium barometz(L) J.Sm) Celsi Ananda; Dezi Handayani; Azwir Anhar; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Cendawan pelarut fosfat merupakan cendawan yang mampu mengubah fosfat yang tidak larut menjadi fosfat bebas, Sehingga dapat diserap oleh tanaman dengan mudah. Cendawan pelarut fosfat diisolasi dari tanah atau rizosfer, atau cendawan endofit. Cendawan endofit dapat ditemukan pada akar, batang atau daun tumbuhan. Akar tanaman merupakan habitat yang baik bagi cendawan pelarut fosfat, antara lain akar tumbuhan pakis simpei (Cibotium barometz (L) J.Sm). Tujuan dari penelitian ini adalah untuk mengisolasi cendawan endofit dari akar tumbuhan pakis simpei, dan untuk mengetahui kemampuan melarutkan fosfatnya. Penelitian dilaksanakan di Laboratorium Biologi Umum FMIPA UNP pada bulan Januari hingga Maret 2023. Sampel akar tumbuhan pakis simpei berasal dari Lembah Harau, Kecamatan Harau, Kabupaten Lima Puluh Kota, Sumatera Barat. Potato Dextrose Agar (PDA) digunakan sebagai media isolasi dan Pikovskaya sebagai uji pelarut fosfat. Sterilisasi permukaan menggunakan NaOCl 0,5%. Hasil penelitian menunjukkan bahwa 6 isolat murni cendawan berhasil diisolasi. Terdapat dua isolat yang memiliki aktivitas pelarut fosfat yaitu AK5 dan AK6. Indeks kelarutan terbesar diperoleh pada hari ketiga untuk isolat AK5 dan AK6. Indeks kelarutan adalah 0,2 dan tergolong kategori lemah.
Keanekaragaman Jenis Capung (Odonata) di Blok Silayang Maninjau Ega Putri Nur Aziza Aziza
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Salah satu kawasan konservasi yang berada di Sumatera Barat pada daerah blok Silayang yaitu Cagar Alam Maninjau. Informasi tentang keanekaragaman jenis capung pada kawasan ini masih belum ada. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis capung (Odonata) yang terdapat di kawasan blok Silayang. Metode yang digunakan dalam penelitian ini yaitu metode jelajah area dan fotografi. Hasil penelitian ini menunjukkan indeks keanekaragaman jenis capung (Odonata) di kawasan Cagar Alam Maninjau Blok Silayang termasuk sedang.
Review Of The Diversity Of Crab (Ocypodidae) In Indonesia Which Can Be Used As A Bioindicator Of Environmental Pollution Kaprian Alsyah Kurnia Kurnia; Rijal Satria
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Indonesia is a country known as a mega-biodiversity country, also known as an archipelagic country, which has a long coastline of 95,181 km, the second longest in the world after Canada. The area of Indonesian sea waters reaches 5.8 million km2, or 71% of the total territory of Indonesia. Thus, Indonesia is a country that has an abundant wealth of biological natural resources, one of which is marine biota from the crustacean group, namely crabs. One of the crab families is Ocypodidae. The crabs of the Ocypodidae family have an important role in the ecosystem. This study aims to see the potential of Ocypodidae crabs as bioindicators based on the results of previous studies. The method used in this research is descriptive research by conducting online surveys through articles from previous studies and from the Crab Database web. A total of four genera of crabs from the Ocypodidae family are found in Indonesia, namely: Austruca (three species), Gelasimus (two species), Ocypode (three species), and Tubuca (three species). We assume that crabs from the Ocypodidae family have the potential to be used as bioindicators of existing environmental pollution.
Orientasi Cahaya pada Pemilihan Sarang Buatan Burung di Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang Tuti Alawiyah
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Sarang merupakan tempat bagi burung untuk meletakkan serta menjaga telur dan anakan hingga mereka dewasa. Penelitian Ini bertujuan untuk mengamati aktifitas dan jenis burung yang tinggal atau memanfaatkan sarang buatan, serta pengaruh orientasi cahaya pada pemilihan sarang burung. Penelitian ini dilaksanakan di sekitar kawasan Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, pada bulan Oktober 2022. Penelitian dilakukan dengan menentukan jenis pohon yang akan dipasang sarang buatan dengan ketinggian pemasangan 2-4 m (di luar jangkauan manusia) serta mencatat arah pintu masuk sarang buatan (barat atau timur). Pengamatan dilakukan dengan mencatat ada atau tidak adanya jenis burung yang memanfaatkan sarang buatan, pengamatan ini dilakukan setiap minggu pada pagi hari pukul 07.00 dan sore hari pukul 16.00. Pada penelitian orientasi cahaya terhadap sarang buatan ini tidak satupun burung yang menghinggapi 16 sarang yang dipasang pada 16 pohon yang berbeda. Hal ini disebabkan karena ada beberapa faktor yang membuat burung tidak menghinggapi sarang buatan yang telah dipasang yaitu terdapatnya sarang semut, aktivitas manusia dan polusi suara di sekitar lokasi pengamatan.
Primer Design and Annealing Temperature Optimization for Catalase (CAT) Gene Amplification in Rice (Oryza sativa L.) Yulita, Nelfi; Violita; Achyar, Afifatul; Putri, Dwi Hilda
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v8i3.222

Abstract

Catalase (CAT) is an antioxidant enzyme that plays an important role in plant growht and defense against abiotic stress, one of which is rice (Oryza sativa). When plants are under abiotic stress conditions, the CAT gene will be regulated and expressed as a form of defense response to stress. To determine the expression of this CAT gene, optimal primers and annealing temperatures are needed to spesifically amplify the CAT gene. This study aims to design primers and determine the optimal annealing temperatures to amplify the CAT gene. Primers are designed using the tools pick primer, primer BLAST and geneious prime. Primers are designed in silico and must meet the ideal primer criteria because they will be used in vitro. Optimization of the annealing temperature was carried out using a PCR temperature gradient. The results of this study obtained a pair of primers, namely forward primer 5’-ATAAGTAGGGCGGTGTGTGG-3’ with a primer length of 20 bp, and reverse primer 5’-GCGAGTTGTTGTTGTTCCATAC-3’ with a primer length 22 bp. This primer pair produces a 185 bp amplicon in the Oryza sativa CAT gene. The optimum annealing temperature for this primer pair is 60ºC.
ANALYSIS SPECIFIC ACTIVITY OF XYLANASE ENZYME PRODUCED BY THE CONSORTIUM OF THERMOPHILIC BACTERIA FROM SAPAN SUNGAI ARO HOT SPRING Feby Yeriska; Mulia; Irdawati; Linda Advinda; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Thermophilic bacteria are bacteria that are able to survive in high temperatures ranging from 45 – 80oC so that they have the potential to produce enzymes that are stable against heat. Thermophilic bacteria can be found in various places in nature that have potential hot springs. One of the potentials of thermophilic bacteria in producing enzymes is the xylanase enzyme. Xylanase is able to hydrolyze xylan (hemicellulose) into xylo-olisaccharides and xylose. In the industrial world, xylanase enzymes are used as paper whiteners, increase the volume of bread, as juice purification, and are used in the manufacture of detergents. This research is a type of descriptive research that aims to determine the specific activity of the xylanase enzyme and the levels of xylanase protein produced by thermophilic bacteria SSA (Sapan Sungai Aro) with bacterial isolates SSA3, SSA4, SSA5, SSA6, SSA7, SSA8, SSA13, SSA14 and SSA15. The results showed that the bacterial isolates SSA3, SSA4, SSA5; isolates SSA6, SSA7, and SSA8; and SSA13, SSA14 and SSA15 respectively had specific activity of the xylanase enzyme with an average value of 0.1614 Unit/mg; 0.1638 Unit/mg and 0.1473 Unit/mg. The final measurement results showed that the highest specific activity of the xylanase enzyme was in the consortium of thermophilic bacteria isolates SSA6, SSA7, and SSA8 with an average of 0.1638 Unit/mg.
Specific Primer Design and Optimization of Monodehydroascorbate reductase (MDHAR) Gene Amplification in Rice (Oryza sativa L.) Jumatul Hafsah; Afifatul Achyar; Zulyusri; Yusni Atifah; Linda Advinda; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Monodehydroascorbate reductase (MDHAR) is an enzyme responsible for growth and response to biotic and abiotic stress. MDHAR in rice shows a higher sensitivity to stress compared to other plants. This study aims to obtain specific primers for the MDHAR gene in rice to be used in PCR amplification so that it can amplify the MDHAR gene. Primers are designed using the Pickprimer and Geneious Primer tools. Optimization of annealing temperature was carried out using the gradient PCR method and then an in vitro primary specification test was carried out using the Touchdown PCR method. The results of the primary design obtained one candidate primer that met the ideal primer requirements, namely a pair of primers (5'-AAAAACACTGCATGGGTCGTC-3' and 5'-CGCCTACCGTTTCCCAAGTT-3') with an amplicon length of 160 bp. The visualization results of PCR products using 1.5% agarose showed that 6 samples were able to amplify the MDHAR gene at 160 bp in size. However, in each lane there is a non-specific DNA band (Primer dimer). In vitro primer specification testing with Touchdown succeeded in increasing product formation specifications and was able to reduce non-specific DNA bands (Primer dimers).

Page 11 of 19 | Total Record : 187

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