cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
biosaintifika@mail.unnes.ac.id
Phone
-
Journal Mail Official
biosaintifika@mail.unnes.ac.id
Editorial Address
D6 Building Floor 1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Sekaran, Gunungpati, Semarang, Central Java, Indonesia 50229
Location
Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : https://doi.org/10.15294/biosaintifika
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika: Journal of Biology & Biology Education {PISSN 2085-191X| EISSN 2338-7610} published scientific papers on the results of biology and biology education research {see Focus and Scope}. Editor accepts the article has not been published in other media with the writing format as listed on page manuscript writing guidelines {see Author Guidelines}. The journal published three times a year, on April, August & December and published by Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in collaboration with Perhimpunan Biologi Indonesia.
Arjuna Subject : Ilmu Sosial - Pendidikan
Articles 18 Documents
 1   2 
Search results for , issue "Vol. 16 No. 2 (2024): August 2024" : 18 Documents clear
Molecular Identification Validates Morphological Identification of Javanese Cardamom from Banyumas in Central Java, Indonesia Sukarsa, Sukarsa; Herawati, Wiwik; Mohamad, Alkhader Ali Mokhaer
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.2324

Abstract

Cardamom is one of the prominent plants with significant economic value in the Banyumas Regency, Central Java, Indonesia. Although Javanese cardamom is traditionally categorized under Amomum compactum, the morphological variations observed create ambiguity about its exact species status. DNA barcoding using the maturase K (matK) and ribulose-bisphosphate carboxylase (rbcL) genes is proven as a reliable technique to elucidate the taxonomic status of morphological variable plant cultivars. This study aimed to characterize cardamom from Banyumas Regency using morphological and molecular approaches for taxonomic status identification and genetic diversity evaluation. The matK and rbcL genes were selected as genetic markers and sequenced using a bidirectional sequencing technique. Morphological examination showed significant color variations at the cardamom stem base. All samples had high genetic identities to reference species in databases and were supported by high query cover and zero e-values. Therefore, molecular characterization, alongside geographic distribution assessment, established that this plant belongs to a single species, Amomum compactum. Additionally, the analysis conducted showed a low level of genetic diversity, as evidenced by haplotype and nucleotide diversity. Low-level genetic diversity provides additional data to convince that cardamon in Banyumas Regency belongs to a single species. These results are essential data in seed selection for further cultivation.
Characterization of Batik Waste Containing Synthetic Textile Dyes and The Artisan Awareness Regarding The Hazardous Batik Waste Hidayati, Yunin; Siswanto, Dian; Rumhayati, Barlah; Retnaningdyah, Catur
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.2325

Abstract

Synthetic textile dyes in the Tanjung Bumi batik coloring process in Madura, Indonesia, have been used extensively for decades, raising environmental concerns due to hazardous wastewater. This research aims to evaluate the quality characteristics of synthetic textile dye wastewater, assess batik artisans' awareness of batik waste hazards, and analyze the interaction between these factors. Wastewater samples were collected from four locations with two repetitions each. Thirty-seven batik artisans participated in a survey. The wastewater analysis included measurements of BOD5, COD, pH, TSS, phenol, Cr, NH3-N, sulfide, and oil and grease. The survey assessed the artisans' use of synthetic dyes and their awareness of environmental hazards. The results showed that the toxic substance levels in Tanjung Bumi batik waste exceed the quality standard thresholds for textile waste. Survey findings indicate that 100% of respondents use synthetic textile dyes, 83.78% dispose of waste without processing, and 91.89% are unaware of the hazards posed by synthetic textile dyes. There is a direct correlation between the artisans' low awareness of the negative impacts of waste and their lack of environmental preservation practices. The novelty of this research lies in its dual evaluation of chemical wastewater characteristics and socio-environmental awareness among artisans. This approach highlights the urgent need for educational interventions and improved waste management practices. The implications are significant for developing sustainable batik production methods and enhancing environmental awareness, contributing to broader societal benefits.
DNA Barcoding of Ornamental Crab Geosesarma in South-Slope Mount Slamet Central Java, Indonesia Winarni, Elly Tuti; Rofiqoh, Aswi Andriasari; Bhagawati, Dian; Pulungsari, Anastasia Endang; Mahmoud, Hanan Hassan Alsheikh; Nuryanto, Agus
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.2376

Abstract

Geosesarma shows intraspecific carapace color variation, which might lead to species misidentification. The problem can be solved using DNA barcoding. There is one research about Geoserarma from the southern slopes of Mount Slamet, but samples were only collected from the Banjaran River for morphological identification. Here, we collected samples from wider areas covering south slope and applied molecular identification. This research aims to assess Geosesarma diversity in south-slope Mount Slamet Central Java, Indonesia based on the cytochrome c oxidase 1 gene barcoding. Surveys were carried out at six sites. Taxonomic identification was done using the barcoding technique. Four morphotypes were obtained during the research. Three morphotypes with the square carapace were identified as Geosesarma, while the remaining one morphotype was included in Parathelphusa. The three Geosesarma morphotypes were barcoded as Geosersarma dennerle because their genetic identity was more than 97% of the G. dennerle sequence in Boldsystems. In contrast, the Parathelphusa morphotype was barcoded as P. convexa with a genetic identity of 97.50%. It can be concluded that the Geosesarma crab on the south-slope Mount Slamet only consists of one species but has carapace and claw color variations. The data are essential for Geosesarma market development and conservation in the region.
The Alteration Level of VEGF of Colon Cancer Cell After Induction with Mandarin Orange (Citrus Reticulata) Peel Ethanol Extract Astuti, Yoni; Zuliana Utami, Anisa; Suharto, Agus; Yano, Yoshihiko; Aristyaning Putri, Wahyu
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3401

Abstract

The natural ingredient-based therapy extracted from various plants is assumed more tolerable to cancer patients’ bodies than synthetic drugs. To tell successful novel cancer therapy, an indicator is needed to confirm the result. VEGF is a pro-angiogenic factor that leads the cancer cells’ invasion to another organ and is associated with high mortality in patients. Hence, the VEGF level is widely used as one of the successful cancer therapy indicators. Purposed of research elucidated the effect of Mandarin orange peel ethanol extract (EEKJM) to improve the reduction of VEGF levels in colon cancer in vitro. This study was an experimental posttest and control group design to analyze different level of VEGF on cancer cells at end of the study.  Research began with the preparation of EEKJM followed by a cytotoxic extract test using the MTT assay method. Furthermore, the measurement of VEGF levels by the VEGF ELISA kit protocol. One Way ANOVA was performed to analyze data.Results showed the cytotoxic test of the EEKJM and doxorubicin against WiDr cells was 240 g/ml and 1.59 µg/ml. VEGF levels showed decreasing after being induced with EEKJM and doxorubicin with doses ½ IC50 and ¼ IC50. Conclusion: EEKJM was able to reduce VEGF levels and viability of WiDr colon cancer cells. The advantages are as much as initial evidence of the ability of the orange to treat cancer even though further animal studies or evidence of a pathway of decrease of the VEGF or other signals are required.
Genetic Diversity of Pacific Seabream, Acanthopagrus pacificus in South-Coast Java, Indonesia Nuryanto, Agus; Bhagawati, Dian; Tuti Winarni, Elly; Wibowo, Dwi Nugroho; Abdalla Mohammed, Mohammed
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3627

Abstract

Pacific Seabream, Acanthopagrus pacificus, is a popular marine fish from the Sparidae family. This species is an economically essential fisheries commodity in the south-coast Java, Indonesia. However, limited studies are available on A. pacificus in Indonesia. Only two studies reported the presence of A. pacificus in South-Coast Java. No study has been carried out on the biology of A. pacificus from south-coast Java. Therefore, biological studies of A. pacificus are needed, including genetic diversity studies. This study aimed to evaluate the genetic diversity of A. pacificus in South-Coast Java using the cytochrome c oxidase gene. Fish samples were bought from Bantul Fishing Harbor, Yogyakarta, Panganadaran Fishing Port, Bojongsalawe Fish Auction Center, West Java, and Binuangeun Fishing Harbor in Lebak Regency, Banten. The genetic marker was processed in PT. Genetika Science Indonesia follows the company procedure. Haplotype and nucleotide diversity were calculated mathematically using Arlequin software. The result showed that polymorphic loci were only 1.3%, indicating low polymorphisms. A. pacificus showed haplotype and nucleotide diversities of 0.511±0.110 and 0.15%±0.12%, respectively. Those values indicated low genetic diversity. This study concluded that the A. pacificus population in South-Coast Java showed low genetic diversity. This study provides the first data about the genetic diversity of A. pacificus in South-Coast Java, which is essential data for fisheries management.
The potency of Elaeocarpus grandiflorus Leaf Extract as Anti-obesity:  in vivo and in silico Study Nugrahaningsih, WH; Ramasamy, Sujatha; Laraswati, Vivi Anggraeni; Yuniastuti, Ari; Christijanti, Wulan
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3695

Abstract

The research aims to determine the effect of ethanol extract of Elaeocarpus grandiflorus leaves extract as anti-obesity by in vivo and silico computational models.  The pre-and -post-tests were carried out on 20 female Wistar rats, divided into 4 groups. The control group (K) received no treatment and the experimental groups were treated with E. grandiflorus extract of 200 mg/kg BW (P1), 400 mg/kg BW (P2), and 800 mg/kg BW (P3) for 14 days. The body weight, abdominal circumference, and abdominal fat mass were measured on Day 1 and Day 15. The results showed differences in body weight (p=0.02), abdominal circumference (p=0.01), and abdominal fat mass (p=0.00). In silico exploration, bioactive compounds of rutin, orientin, luteolin, vitexin, iso orientin, isovitexin, kaempferol, and quercetin were identified, and targeted ELAVL1, IGF1R, CREB1, AKT1, and PIK3R1 of the AMPK signaling pathway that involved in the anti-obesity mechanism. The high binding affinity values was rutin-EVAL1 (-9.3), orientin-ELAVL1 (-8.3), and quercetin- IGFR1 (-8.2). It can be concluded that the ethanol extract of E. grandiflorus leaves has the potential to be developed as an anti-obesity agent.
Unveiling the Enigmatic Dwarf Horseface Loach Acanthopsoides molobrion: A Groundbreaking Discovery in Indonesia Fitri Sil Valen; Denny Syaputra; Ismail, Mohd Hasmadi; Swarlanda, Swarlanda; Kamarudin, Ahmad Syazni; Veryl Hasan; I, Itaya
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.5671

Abstract

Acanthopsoides molobrion is a freshwater previously known to be spread across Malaysia and Borneo. This study aimed to record for the first time the occurrence of the A. molobrion in Bangka Island, Indonesia, and update the geographic distribution of this species. This new record of freshwater fish provides an important contribution to the comprehension of the biogeography of the species. The purpose sampling approach was employed for this study to collect specimens. The species were then identified morphologically by the application of morphometric and meristic methods, and molecularly through the employ of DNA Barcoding. On 20th January 2023, two A. molobrion specimens were collected from the Bumang Kemuja River, Bangka Island using a fish trap. The new record of A. molobrion found here is the southernmost record for this species, expanding its geographic distribution. In addition, the latest record site is about 500 km south of the nearest locality in Peninsular Malaysia, and about 750 km southwest of the nearest locality in Borneo. The new record of A. molobrion has expanded the species' recorded distribution range, which has added to our understanding of this species. Furthermore, we present an updated record for the A. molobrion DNA sequence based on the COI gene. This sequence is the first DNA Barcode to Indonesia. Subsequently, the DNA sequence was registered into NCBI Genbank with the access code OR144414. This DNA barcode will be used as a standard for identifying A. molobrion and will aid in DNA and biotechnology-based studies in the future.
The Role of Sembung (Blumea balsamifera) Leaf Extract in Preventing Atherosclerosis in Hyperlipidemia Rat Models I Made Jawi; I Wayan Putu Sutirta Yasa; I Gede Widhiantara; Anak Agung Ngurah Subawa; Putu Angga Wiradana; Naw, Sin War
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6673

Abstract

This study aims to prove that administering BBLE as a natural antioxidant can prevent atherosclerosis by maintaining lipid profiles, antioxidant enzymes, and netrin-1 levels in hyperlipidemia in rat models. The research subjects were 20 adult male Wistar rats (Rattus norvegicus), which were divided into 2 groups using a randomized pretest and posttest control group design. Before treatment and after treatment for 3 months, lipid profiles, MDA, SOD, and netrin-1  were examined. The control group was only given high-cholesterol diets (HCD), while the treatment, apart from HCD, was also given BBLE 4mg/day. The data obtained was tested using paired t-test and group t-test. The results of the study showed that there was a significant decrease in netrin-1 in the control group (p<0.05) after being given HCD for three months. In the treatment group, it also decreased but it was not significant (p>0.05). Netrin-1 levels in the treatment group were higher than the control (p<0.05). The lipid profile experienced a significant increase in HDL in the treatment group accompanied by a significant decrease in MDA and an increase in SOD (p<0.05) when compared with the control group. This study concludes that administering BBLE at a dose of 4 mg/day to rats given HCD caused an increase in netrin-1 levels accompanied by improvements in lipid profiles and prevention of oxidative stress. The findings of this study reveal the novelty of BBLE in treating and maintaining blood vessel function in mice given HCD by increasing netrin-1 levels.
Antibacterial Production by Endophytic Bacteria from Catharanthus roseus in East Timor Against Methicillin-Resistant Staphylococcus aureus  Missa, Hildegardis; Ndukang, Sardina; Djalo, Aloysus; Nau, Getrudis W.; Susilowati, Ari; Baunsele, Anselmus Boy; Santos, Americo Dos
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.7675

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) refers to a group of bacteria that cause infections and present a challenge in treatment due to their resistance to several antibiotics. Therefore, research is needed to discover endophytic bacteria from Catharatus roseus capable of producing new antibiotics. This study aims to identify endophytic bacteria from C. roseus plants originating from Timor Island, as producers of effective antibacterial compounds against MRSA. Antibacterial activity tests were conducted on two types of test bacteria, namely MRSA and Escherichia coli. DNA extraction was performed using the PrestoTM Mini dDNA kit Bacteria, and amplification of the 16S rRNA gene was carried out. The research found bacterial isolates showing morphological similarities to Bacillus sp. Screening results indicated that four bacterial isolates exhibited high potential antibacterial activity against MRSA, as evidenced by the formation of inhibition zones with diameters of approximately 25 mm-35 mm. Amplification of the four endophytic bacterial isolates from C. roseus identified them as Paenibacillae FaCH2, Bacillae BoCH3, Aneurinibacillae BoCH5, Aneurinibacillae BiCH8, which are new species based on 16S rRNA gene similarity of less than 97%. In conclusion, endophytic bacteria from C. roseus producing antibacterial compounds against MRSA have been successfully identified. This is beneficial to society because the antibacterial compounds produced can serve as a basis for developing new drugs that are effective against MRSA infections, considering the increasing antibiotic resistance against MRSA.
Content Analysis of Bioenergetics’ Conceptual Presentation in Senior High School Biology Textbooks Alforque, Jonemel M.; Jovero, Marijul B.; Bodanio, Cenando; Ecle, Niño; Picardal, Jay P.
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.3399

Abstract

Factors that bring about a low mastery level of bioenergetics, a core concept in biology, include relatively poor teaching-learning experience and low-quality textbooks, among others. To determine which textbook and teaching components have affected the low learning mastery of the topics in bioenergetics, the researchers analyzed the textbooks’ quality of content, language, layout, and figures, and the teachers’ experience, contentment, and challenges while teaching the topic, and effects to the learners. Using a sequential explanatory mixed method research design, ten (10) textbooks, and seven (7) teacher participants were purposively selected following a set of criteria. Descriptive and principal component analyses revealed that the textbooks' quality depends on two components: (1) competent and coherent visuals and content, and (2) consistency of style in the varied forms of assessment. Narrative analysis and Quasi-Statistics analysis revealed that teachers are not contented with the textbooks as they are inadequate to teach the subject matter. The lack of good learning materials, coupled with low competence to introduce the topics, resulted in the low mastery level of the learners. The researchers propose that periodic textbook assessment, retooling of teachers’ skills, and deepening teachers’ understanding are necessary to improve learners’ performance in bioenergetics. Finally, this paper is instrumental in providing educators, resources and curriculum developers, and researchers a feasible and practical path to improve textbook quality and address pedagogical gaps.

Page 1 of 2 | Total Record : 18

 1   2 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol. 17 No. 3 (2025): November 2025 Vol. 17 No. 2 (2025): August 2025 Vol. 17 No. 1 (2025): April 2025 Vol. 16 No. 3 (2024): December 2024 Vol. 16 No. 2 (2024): August 2024 Vol. 16 No. 1 (2024): April 2024