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Bioeksperimen
Published by Universitas Muhammadiyah Surakarta
ISSN : 24601365     EISSN : 25272799     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Aim Bioeksperimen: Jurnal Penelitian Biologi is a peer-reviewed, open-access scientific journal dedicated to advancing knowledge across a broad spectrum of biological sciences through the publication of high-quality original research articles. The journal promotes rigorous experimental and exploratory research that advances fundamental and applied understanding in biological sciences. Bioeksperimen provides an international platform for researchers, academics, and practitioners to disseminate innovative empirical findings and scientifically robust investigations that contribute to the development of biological sciences at regional and global levels. The journal welcomes manuscripts that: 1. Present original experimental or exploratory research in biological sciences 2. Demonstrate strong methodological design and scientific rigor 3. Contribute novel data, findings, or biological insights 4. Address contemporary biological challenges and emerging scientific issues 5. Integrate laboratory, field, and computational approaches 6. Contribute meaningfully to the advancement of biological sciences All manuscripts must be original, unpublished, written in English, and must clearly demonstrate methodological soundness, data-driven analysis, and a significant contribution to biological sciences. Since March 2026, Bioeksperimen does not publish literature review articles, conceptual papers, or purely theoretical manuscripts. Focus The primary focus of Bioeksperimen: Jurnal Penelitian Biologi is on empirical biological research based on experimental, observational, or exploratory approaches. The journal prioritizes studies that: 1. Apply molecular, physiological, ecological, microbiological, or genetic techniques 2. Utilize omics-based and bioinformatics approaches to generate primary data 3. Conduct laboratory experiments, field investigations, or integrative biological exploration 4. Produce reproducible and evidence-based scientific findings Scope Bioeksperimen: Jurnal Penelitian Biologi publishes original research in the following areas: Main Topics: 1. Ecology and Environmental Sciences 2. Molecular Biology of Plants, Animals, and Microorganisms 3. Bioinformatics and Computational Biology 4. Omics-Based Studies (Genomics, Transcriptomics, Metagenomics, Proteomics, Metabolomics) 5. Basic and Applied Microbiology 6. Plant and Animal Physiology 7. Animal Behavior 8. Plant and Animal Systematics and Taxonomy 9. Genetics of Plants, Animals, and Microorganisms 10. Human Physiology
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 20 Documents
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Search results for , issue "March 2026" : 20 Documents clear
Utilization of cassava peel waste (Manihot esculenta) as substrate in the production of cellulase enzymes by Aspergillus niger Hendriantika, Ratu Dewi; Peristiwati; Surtikanti, RR. Hertien Koosbandiah
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.10369

Abstract

Cellulase enzymes is an enzyme that can hydrolyze cellulose into smaller sugar components. Cellulase enzymes are produced due to high demand, with 29.17% in the animal feed industry, 14.67% in the textile industry, and 26.17% in the paper industry. One method of producing cellulase enzymes is by utilizing the cellulolytic microorganism Aspergillus niger. This study aims to optimize cellulase enzyme production by Aspergillus niger through liquid fermentation on cassava peel substrate (Manihot esculenta) with pH treatments of 4.5 and 5.5 and temperatures of 29.5 and 30.5℃. The research method involved isolating pure cultures of Aspergillus niger from the laboratory of PT. Agritama Sinergi Inovasi. The fungus was grown on CMC agar medium to test its cellulolytic activity. The tested cellulolytic fungus can be used in fermentation in a medium containing cassava peel powder as the main cellulose substrate. The results of this study indicate that the cellulolytic fungus Aspergillus niger has the potential to produce cellulase enzymes. The highest enzyme activity value of 2.003 U/mL with a biomass of 0.535 mg/mL was obtained at pH 4.5 and 30.5℃. This study provides an important contribution in assessing the potential for cellulase enzyme production with variations in pH and temperature by Aspergillus niger fungi on cassava peel substrate as a basis for further development in organic waste management.
Antibacterial activity of miana (Coleus scutellarioides) against Streptococcus pyogenes and Streptococcus mutans Aprillia, Klara; Sianturi, Sister
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.9152

Abstract

Miana leaves (Coleus scutellarioides) have compounds that have the potential to be antibacterial. The bacteria Streptococcus pyogenes and Streptococcus mutans are a group of positive bacteria containing a lipid layer that is low in peptidoglycan and teichoic acid. This study aims to determine the antibacterial activity of Miana leaves against Streptococcus pyogenes and Streptococcus mutans because both types of infectious bacteria. This research used the disc diffusion method. The preparation involved the maceration method using 96% ethanol as a solvent for five days, followed by concentration using a water bath at 70°C to obtain a thick extract. Observations were made on the diameter of the inhibition zone visible in the clear zone around the disc. The average inhibition diameter by ethanol extract against S. pyogenes is 9.83 mm, 10.76 mm, 12.42 mm, 15.66 mm, and 28.56 mm, respectively at extract concentrations of 5, 10, 20, 40, and amoxicillin (positive control). The negative control 1% DMSO did not form an inhibition zone. The statistical analysis using One-Way ANOVA (SPSS 27) yielded a p-value of < 0.05, confirming a significant difference in antibacterial activity across all tested groups. This result demonstrates that Miana leaf ethanol extract at concentrations of 5%, 10%, 20%, and 40% effectively inhibits the growth of both S. pyogenes and S. mutans. Furthermore, the inhibition zones varied significantly between the extract concentrations and the control groups, proving that the extract’s efficacy is concentration dependent. These findings suggest that Miana leaf ethanol extract has potential as a natural antibacterial agent for developing alternative phytotherapeutic treatments against S. pyogenes and S. mutans infections.
Synergistic cultures for resilient mercury bioremediation in ASGM leveraging microbial interactions for sustainable pollutant removal Wibowo, Sekar; Purwanti, Ipung Fitri; Firdaus, Abiyyu Armijn Firman
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.11967

Abstract

The widespread use of mercury (Hg) in artisanal and small-scale gold mining (ASGM) necessitates effective bioremediation strategies. This study evaluated the mercury reduction capabilities of two bacterial strains, Bacillus subtilis and Pseudomonas aeruginosa, using real-world ASGM liquid waste. In a seven-day laboratory-scale experiment, we assessed the performance of single and mixed bacterial cultures under varying pH conditions. Our results show that B. subtilis was particularly effective, achieving a maximum Hg2+ removal efficiency of 90.07%. Critically, while the cell viability of single cultures declined significantly over the study period, mixed cultures maintained superior population stability, reaching 7.4 log CFU/mL on day 7, especially under alkaline conditions. This stability suggests a beneficial synergistic relationship between the two species. The enhanced long-term viability and robust detoxification mechanisms observed in the mixed culture system underscore the high potential of this approach for developing sustainable bioremediation solutions for the ASGM sector.
A Vegetation analysis of terestrial fern in kemuning tea garden in ngargoyoso village, Indonesia Yasmin, Hasna Aulia; Roziaty, Efri
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.12195

Abstract

Kemuning Tea Garden in Ngargoyoso Village is a beautiful area with many tourist destinations. Therefore, in addition to the activities of local people as tea farmers, many outsiders come as tourists. This area has abiotic factors that are suitable for the growth of various types of plants, one of which is ferns, especially terrestrial ferns. This study aims to determine the composition of terrestrial fern vegetation in the kemuning tea garden area. The method used was purposive sampling method, with 6 plots in 3 Stations with different heights: Station 1 (800 - 900 m asl), Station 2 (900 - 1000 m asl), and Station 3 (>1000 m asl). This study obtained 9 species of terrestrial ferns: Pityrogramma calomelanos, Adiantum raddianum, Pteris ensiformis, Pteris biaurita, Christella dentata, Nephrolepis exaltata, Dicranopteris linearis, Lycopodiella cernua, and Dryopteris dilatata; that belong to 3 families: Polypodiaceae, Gleincheniaceae, and Lycopodiaceae. The study results show that the type of Nephrolepis exaltata gets the highest INP value, which is 92,88 %. While the lowest INP index is obtained by Pityrogramma calomelanos, which is 9,52 %. The diversity index obtained by the Shannon-wiener formula is 1,85; which means that the diversity in Kemuning Tea Garden is moderate. These findings highlight the importance of adaptive vegetation management in tea plantation landscapes to maintain fern diversity while supporting sustainable agroecosystem functions.
In vitro antifungal activity of Mimosa pudica rhizosphere bacteria against Fusarium spp. Sriwulan, Sriwulan; Mustikaningrum, Dhina; Nurfitria, Nia
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.13509

Abstract

This study aims to explore rhizosphere bacteria from Mimosa pudica L. as a source of antagonistic bacteria against Fusarium spp. Rhizosphere soil samples were collected, isolated, morphologically characterized, and tested for antifungal activity in vitro against Fusarium spp. The percentage of inhibition (PI) was calculated after 7 days. Six bacterial isolates were obtained and tentatively identified as Bacillus, Cellulomonas, Arthrobacter, and Micrococcus species. All isolates exhibited antagonistic activity against Fusarium spp., with inhibition percentages ranging from 61.99% to 81.83%. Isolate PM2 (tentatively identified as Cellulomonas) demonstrated the strongest inhibition at 81.83%, followed by isolate PM6 (tentatively identified as Micrococcus) at 79.25%. These results confirm the potential of Mimosa pudica L. as a source of biocontrol agents and warrant further investigation into their molecular identification, mechanisms of action, and in vivo efficacy.
Integrating BSF larvae for the sustainable bioconversion of banana peels and sprout hulls waste Rizkawati, Vina; Andhini, Shabrina Lathiifah; Noer, Mohamad Isnin; Yudistira, Dwi Harya; Kurniati, Tri Handayani; Lisanti, Elsa; Komala, Ratna; Herlin, Weri
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.13637

Abstract

The management of organic waste presents a significant challenge in Indonesia, especially fruit and vegetable by-products. Black Soldier Fly (BSF, Hermetia illucens) larvae offer an effective solution due to their high potential for organic waste conversion. This study investigated the efficacy of BSF larvae in reducing and bioconverting organic waste composed of banana peels and sprout hulls, and examined the influence of EM4 fermentation on larval growth performance. Waste Reduction Index (WRI) and bioconversion rate (BCR) were calculated as percentages and analyzed descriptively, followed by the Kruskal–Wallis test. Furthermore, larval length and body weight were measured at 7, 11, 14, and 18 days of larval age and analyzed using Kruskal–Wallis with Dunn’s post-hoc test. The results showed that EM4–fermented substrates containing a 1:3 ratio of banana peels to sprout hulls showed the highest waste reduction and bioconversion potential among all mixture treatments (P < 0.05). Although larval length and weight were lower than control substrate, no significant difference in larval length was detected, while individual larval weight was significantly higher compared to other organic waste combinations (P < 0.001). In this study, EM4 fermentation enhanced lignocellulosic degradation, improving substrate digestibility for BSF larvae and optimizing bioconversion efficiency, thus supporting more sustainable organic waste management strategies.
The Phylogenetic analysis of representative mammalian MUC16 supported by comparative SEA domain and tandem repeat variation Foenna, Yudi Gebri; Yulisma, Ardhana; Calapoğlu, Nilüfer Şahin
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14233

Abstract

MUC16 is one of the largest mammalian mucins and exhibits substantial evolutionary variation in both sequence composition and structural modularity. Comparative analysis of SEA domain composition and tandem repeat architecture is therefore essential for understanding the evolutionary diversification of this gene across mammals. This study investigates the phylogenetic relationships of mammalian MUC16 and examines how variation in SEA domains and tandem repeats contributes to lineage-specific structural divergence. MUC16 nucleotide and protein sequences from 20 mammalian species representing Primates and Rodentia were retrieved from public databases. Multiple sequence alignment and phylogenetic reconstruction were conducted using the Neighbor-Joining method with 1,000 bootstrap replicates. SEA domains were annotated using the SMART database, while tandem repeats were identified with Tandem Repeats Finder. Structural features were evaluated using descriptive statistics, hierarchical clustering, and Spearman’s rank correlation analysis. Phylogenetic reconstruction revealed a clear molecular separation between Primates and Rodentia with strong bootstrap support. Primate species generally exhibited conserved sequences and expanded SEA domain and tandem repeat architectures, whereas rodents displayed higher sequence divergence and reduced structural complexity. A moderate positive association between SEA domain number and tandem repeat count (ρ = 0.44) was observed, although this relationship did not reach statistical significance and is therefore interpreted as a biologically suggestive trend rather than evidence of coordinated evolution. Overall, the results indicate that MUC16 evolution follows lineage-dependent patterns shaped by both sequence divergence and domain-level remodeling. This comparative framework provides an evolutionary context for understanding structural diversity in mammalian mucins and offers a foundation for future functional and genomic investigations.
Isolation and screening of halotolerant protease-producing bacteria from Lampung fermented shrimp paste Istiadi, Khaerunissa Anbar; Ningsih; Suryanti, Erma; Imaniar, Lisana Husna
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14593

Abstract

Protease are class of degradative enzymes that hydrolyze proteins, breaking its peptide bond, into smaller peptides and amino acids. Microbial proteases have gained increasing attention due to its productivity and utilization in various industries. Proteolytic bacteria derived from fermented shrimp paste from Lampung have great potential to meet this need. However, studies on these bacteria are still limited, so further research is needed to explore and optimally utilize their potential. This study aims to explore the diversity of proteolytic bacteria from Lampung terasi.. The methods employed included isolation stages using Nutrient Agar supplemented with 5% and 10% NaCl (w/v), followed by proteolytic screening properties using Skim Milk Agar media. A total of 20 bacterial isolates were obtained from terasi using Nutrient Agar supplemented with 5% (w/v) NaCl, and 15 isolates were obtained from Nutrient Agar (NA)  supplemented with 10% (w/v) NaCl. Screening results showed that 85% and 67% of the isolates obtained from NA supplemented with 5% and 10% NaCl, respectively, exhibited proteolytic activity. Halotolerant bacterial isolates isolated using NA + 5% NaCl exhibited a proteolytic index ranging from 0.3 – 3.0, while those isolated with NA + 10% NaCl showed values ranging from 0.18 to 3.06. The screening results revealed that isolates BTCL5-07 and BTCL10-05 exhibited the highest proteolytic activity, with proteolytic indices of 3.0 and 3.06, respectively. These findings indicate that halotolerant bacteria from Lampung terasi represent a promising source of potential proteolytic enzymes. Further research is required to optimize enzyme production.
Morphological and genetic identifications of Spirulina based on 16S rRNA gene Dito, Bahana Safiria; Sajidan, Sajidan; Susilowati, Ari; Sidiq, Yasir
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14879

Abstract

Spirulina is a microalga with spiral or linear trichome shapes that has potential in industrial and food sectors due to its rich nutritional content. Spirulina can live in various aquatic habitats with different environmental conditions. Differences in cultivation environments can affect the morphological characteristics of Arthrospira due to phenotypic plasticity, making morphology-based identification prone to errors. Therefore, molecular identification using the 16S rRNA gene for phylogenetic analysis is necessary. This study aims to identify and analyze the genetic relationships of Spirulina from freshwater and brackish water based on 16S rRNA gene sequences. Freshwater Spirulina samples were taken from Bogor (BGR) and Yogyakarta (MRP), while brackish water Spirulina was taken from Jepara (JPR). DNA extraction was performed using the Zymo Research Quick-DNA Fungal/Bacterial Miniprep Kit, amplification of the 16S rRNA gene was conducted through PCR, followed by DNA sequencing. Phylogenetic analysis was carried out using MEGA11 to construct the phylogenetic tree. The morphology of strains BGR and MRP showed straight and thin filaments, whereas JPR had spiral and relatively long filaments. Sequencing analysis results showed that strains JPR and MRP had the closest homology similarity to Arthrospira sp. IAQUASC-C0001 with percentages of 99.34% and 99.12%, respectively. Meanwhile, Spirulina from BGR had the closest homology similarity to Arthrospira fusiformis strain AICB 668 with a percentage of 99.78%. Phylogenetic analysis indicated that strains BGR, JPR, and MRP are more closely related to Arthrospira than to Limnospira.
Potential of butterfly pea (Clitoria ternatea) nanoherbal on spermatozoa quality in hyperglycemic rats Putri, Komala Amelia; Christijanti, Wulan; Marianti, Aditya
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15239

Abstract

Hyperglycemia is a condition characterized by elevated blood glucose levels that induce free radical formation, leading to reduced spermatozoa quality. Butterfly pea (Clitoria ternatea) contains antioxidant compounds and is formulated as a nanoherbal to enhance bioavailability. This study aimed to evaluate the potential of butterfly pea nanoherbal in improving spermatozoa quality in hyperglycemic rats. This experimental study employed a post-test only control group design using 25 male Wistar rats divided into a healthy control group and hyperglycemic groups induced by alloxan (125 mg/kg BW). The hyperglycemic groups consisted of a metformin control (400 mg/kg BW) and butterfly pea nanoherbal treatment groups at doses of 12.5, 25, and 50 mg/kg BW administered for 28 days. Data were analyzed using One-Way ANOVA followed by the Least Significant Difference (LSD) post hoc test. The results showed that spermatozoa concentration in the healthy control group differed significantly from the positive control, P1, and P2 groups (p<0.05), but not from the P3 group. Spermatozoa motility and viability also showed significant differences among groups (p<0.05). It can be concluded that butterfly pea flower nanoherbal significantly improves spermatozoa quality in hyperglycemic rats.

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