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Buitenzorg: Journal of Tropical Science
Published by Innovation Centre for Tropical Science
ISSN : -     EISSN : 30630150     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions
Buitenzorg: Journal of Tropical Science provides a rapid publication of full-length Research Papers, Short Communication, and Review articles describing new findings or theories in a broad range of topics, including, but not limited to, tropical biodiversity, agriculture, biology, ecology, environmental science, forestry, climate, and health sciences. The scope of Buitenzorg: Journal of Tropical Science includes; > Tropical Biodiversity (animals, plants, microbe and others) > Tropical Plant Biology > Tropical Agriculture > Tropical Ecology > Tropical Climate > Tropical Environment > Tropical Forest > Tropical Diseases > Biotechnology
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 20 Documents
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Pengaruh pemberian pakan fermentasi ampas kelapa dan bekatul padi menggunakan Rhiodiotorula taiwanensis UNJCC Y-171 terhadap pertumbuhan larva Black Soldier Fly (BSF) dan komposisi proksimatnya. Sari, Yunita; Rini Fahmi, Melta; Sukmawati, Dalia
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.14

Abstract

Black soldier fly (BSF) larvae, or maggots, are a promising alternative protein-rich feed for aquaculture. This study aims to evaluate the effect of fermented feed made from coconut pulp and rice bran using the yeast Rhodotorula taiwanensis UNJCC Y-171 on maggot growth and proximate composition. The study was conducted from June to August 2024 at the Universitas Negeri Jakarta and the Ornamental Fish Aquaculture Research Center. The treatments included a control group (K) and fermentation using yeast at 30% (A) and 40% (B) concentrations. Measurements were taken on days 10, 13, 16, and 19. The results showed that fermented feed significantly increased maggot wet weight and body length, particularly in treatment B. On day 16, the wet weight of maggots in treatment B reached 182.00±45.14 g, higher than the control (77,00±52.03 g). The body length of maggots in treatment B was also greater (1.59±0.12 cm) than in the control (1.19±0.09 cm). Proximate analysis indicated that maggots in treatment B had a higher fat content (15.57%) compared to the control (12.11%), while crude fiber content was lower (1.27% compared to 1.63%), indicating improved feed efficiency. Although the protein content in treatment B (12.29%) was slightly lower than in the control (13.27%), fermentation with R. taiwanensis UNJCC Y-171 enhanced nutrient bioavailability. This study confirms that feed fermentation with R. taiwanensis UNJCC Y-171 at a 40% concentration optimally improves both maggot growth and nutritional quality. Therefore, this method has the potential to serve as a more cost-effective alternative feed for aquaculture. Keywords: Rhodotorula taiwanensis, feed fermentation, maggot, proximate analysis, feed efficiency
Karakterisasi Morfologi dan Optimasi Kultur In Vitro Aksesi Ubi Jalar Daging Ungu (Ipomoea batatas L. Poiret) Sabda, Muhamad; Margareth, Clarencia
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.15

Abstract

Purple-fleshed sweet potato (Ipomoea batatas L. Poiret) is a functional food crop known for its high anthocyanin content and nutritional value. This study aimed to characterize the morphological traits of stem and leaf organs from seven purple-fleshed sweet potato accessions and to evaluate the effectiveness of in vitro culture techniques for their conservation. Morphological traits were assessed using a standardized scoring system and analyzed using Principal Component Analysis (PCA) and cluster analysis. Results showed significant variation among accessions, with Ungu Lonjong appearing genetically distinct, while Ayamurasaki, KT Lampa, and Local NTT exhibited high similarity. In vitro culture analysis involved the sterilization and cultivation of explants on Murashige and Skoog (MS) medium and minimal growth medium. The highest sterilization success rate was observed in Ungu Lonjong (78.3%), whereas Local NTT showed the highest contamination. This study demonstrates the potential of combining morphological data and tissue culture for effective characterization, conservation, and future purple-fleshed sweet potato germplasm breeding.   Keywords: Ipomoea batatas, germplasm conservation, morphological characterization, tissue culture
Micromorphological Characterization and DNA Barcoding of Durio macrantha Kosterm. from the Bogor Botanical Garden Collection Magandhi, Mahat; Rusydina, Diyana Sabila; Mukaromah, Arnia Sari
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.16

Abstract

Durio macrantha is an endemic species first discovered in 1981 in Gunung Leuser National Park, North Sumatra, and has been conserved at the Bogor Botanical Garden since 1994. This study aims to characterize the micromorphology and DNA profile D. macrantha using DNA barcoding with rbcL and ITS gene markers. Sample of D. macrantha were collected from the Bogor Botanical Gardens for analysis. Micromorphological analysis revealed that the leaves exhibit three vein types (primary, secondary, and tertiary) with a pentagonal venation pattern and a tri-veinlet veinlet pattern. Additionally, eight types of trichomes were observed on the abaxial surface. Molecular characterization showed that the rbcL sequence was 563 bp long, with nucleotide composition T (27.9%), C (21.7%), A (27.9%), and G (22.6%). The ITS sequence was 960 bp long, with nucleotide composition T (15.4%), C (33.4%), A (19.0%), and G (32.2%). BLAST analysis of both rbcL and ITS genes revealed a high level of similarity between D. macrantha and D. zibethinus. This study provides fundamental data supporting the conservation and further research of D. macrantha, particularly in morphological and molecular aspects.
Karakterisasi dan Kelayakan Ekonomi Citrus aurantifolia kultivar ‘Nimas Agrihorti’ sebagai Komoditas Biofarmasi Potensial di Indonesia Budiyati, Emi; Waluyo, Budi; Maghfoer, Moch. Dawam; Kosmiatin, Mia; Triwiratno, Anang; Hanif, Zainuri; Andrini , Anis; Hanim, Faridha; Tina , Siti Agus
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.17

Abstract

‘Nimas Agrihorti’ is a citrus cultivar developed to fulfil the increasing domestic demand for biopharmaceutical-grade citrus in Indonesia. Its development aligns with the growing public interest in health-promoting agricultural products. This study aimed to evaluate the morphological characteristics, chemical composition, and economic viability of ‘Nimas Agrihorti’ as a biopharmaceutical citrus. Field observations were conducted from June 2019 to October 2020 at the Banjarsari Experimental Field, Bayeman Village, Probolinggo, East Java. Morphological assessments followed the Descriptor List for Citrus by IPGRI, and chemical analyses were performed at the Post-Harvest Laboratory of Brawijaya University, Malang. Economic feasibility was assessed by calculating production costs and revenues over a 2-year cultivation cycle. ‘Nimas Agrihorti’ exhibited favorable morphological traits, including large fruit size (72–82 g), yellow skin, and sweet taste. Chemical analysis revealed vitamin C content of 34.8 mg/100 g and low acidity at 0.45%. The total production cost over 2 years was IDR 42,640,000.00 (equivalent to USD 2,584), with total revenue amounting to IDR 77,360,000.00 (equivalent to USD 4,688), resulting in a Revenue-Cost (R/C) ratio of 2.81. The combination of desirable morphological and chemical characteristics, along with strong economic returns, indicates that ‘Nimas Agrihorti’ holds significant potential for development as a biopharmaceutical citrus cultivar in Indonesia.   Keywords: citrus, cultivar, biopharmaceutical products, economic feasibility
Modulasi pembungaan untuk efisiensi pemuliaan dan optimalisasi biomassa: Tinjauan molekuler dan bioteknologi Polosoro, Aqwin
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.18

Abstract

Regulation of flowering time is a critical determinant of plant reproductive success and a key trait for optimizing crop adaptation, yield stability, and breeding efficiency. This review highlights recent advances in the molecular pathways controlling flowering, including photoperiod sensing, vernalization and temperature response, autonomous and hormonal regulation, and floral integrator networks. Key genes such as FT, SOC1, FLC, TFL1, and Ghd7 serve as central nodes within these interconnected pathways. The application of genetic engineering tools—including gene overexpression, CRISPR/Cas-mediated knockouts, promoter editing, and transient expression systems—has enabled precise manipulation of flowering phenology across a wide range of crops. These strategies have accelerated fast-track breeding in temperate and tropical perennials and facilitated the enhancement of vegetative biomass in forage and industrial crops through delayed flowering. However, the deployment of flowering-modified genotypes presents challenges, including environmental interactions, phenological trade-offs, biosafety regulation, and potential ecological impacts. Future directions should emphasize the integration of flowering time control with speed breeding platforms, genomic selection, and climate-adaptive trait design, tailored to species—and region—specific requirements. Such multidisciplinary approaches will be vital to advancing crop resilience, productivity, and sustainability under changing environmental conditions.   Keywords: flowering time regulation, genetic engineering, FT gene, fast-track breeding, biomass optimization
Studi Dasar Nepenthes spp. di Taman Wisata Alam Bukit Tangkiling, Palangka Raya, Kalimantan Tengah, Indonesia Rizki, Muhammad; Oktafiani, Helen; Panjaitan, Desimaria
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i2.19

Abstract

Pitcher plants (Nepenthes spp.) are carnivorous plants that flourish in the Bukit Tangkiling Nature Tourism Park (BTNTP), Palangka Raya, Central Kalimantan. Searches of global biodiversity databases and relevant literature revealed no prior records of Nepenthes from this area. This study aimed to document and provide a baseline record of Nepenthes species occurring within BTNTP. The study was conducted from March to May 2022 using exploration and purposive sampling methods to collect plant specimens. Three species, namely Nepenthes gracilis Korth, Nepenthes mirabilis (Lour.) Druce, and Nepenthes rafflesiana Jack, were recorded across various habitats, including scrub forest, heath forest, secondary forest, and peat forest, all below 100 m above sea level. These findings contribute to the species inventory of BTNTP and serve as a scientific reference for future ecological monitoring and management strategies aimed at maintaining habitat quality and supporting long-term Nepenthes conservation efforts in the region. Keywords: habitat, species record, pitcher plants, Nepenthes, Kalimantan
Struktur Komunitas dan Asosiasi Crinoidea (Echinodermata) dengan Bentuk Kehidupan Terumbu Karang di Lereng Terumbu Pulau Pramuka, Kepulauan Seribu Rasyid, Muhammad Emir; Patria, Mufti
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i2.20

Abstract

Class Crinoidea, or sea lilies, are primitive members of the Echinoderm phylum, playing a crucial ecological role as filter feeders and indicating ecosystem health. Pramuka Island is the administrative center of the Seribu Islands Regency, DKI Jakarta, which is experiencing rapid development and becoming a promising tourism site. These anthropogenic activities may influence the survival of the coral reef ecosystem below the island. Thus, further research on crinoid communities and their relationship with coral reef lifeforms is needed to assess the health of the reef ecosystem amidst increasing anthropogenic activities. The research was done using the belt transect method covering an area of 50×5 m at a depth of 10 m at four research stations, calculating the diversity index, evenness index, dominance index, and density. The association of Crinoidea with coral reef lifeforms was analyzed using the chi-square test. The result showed poor value with an average low diversity index, moderate evenness index, and high dominance index. Capillaster sentosus, the most abundant species at every station, indicates habitat suitability rather than ecological disturbance, and serves as a potential bioindicator for Pramuka Island’s reef health, though its dominance might signal a decline in other species due to competition. There were six pairs of Crinoidea–substrates found to be positively associated, and four pairs of Crinoidea–substrates that were negatively associated. The associations between crinoids and coral reefs, influenced by crinoid morphology, reef stability, and current flow, highlight the need for conservation efforts to preserve these crucial relationships for biodiversity. Keywords: association, community structure, coral reef, crinoids, Pramuka island
Suplementasi CO2 Membuka Potensi Biomassa, Lipid, dan Karotenoid pada Mikroalga Coelastrella sp. strain Saripa Akhmad Ikhsan, Maulana; Nyoman Semad, Antara; I Wayan , Arnata; Ni Wayan Sri, Agustini; Susanti, Hani
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Microalgae are a group of photosynthetic microorganisms widely distributed in various habitats, especially in aquatic environment. This study aimed to evaluate the effects of carbon dioxide (CO2) gas supplementation on the growth and biomass production of the microalgae Coelastrella sp. strain Saripa, as well as the lipid and carotenoid contents produced. Additionally, color changes during the growth process were observed as physiological indicators of the microalgae's response to CO2 treatments and pH changes during growth. The experiment was conducted using a Completely Randomized Design. CO2 was supplemented at 0, 25, and 50 ml volumes. The results showed that different CO2 supplementations influenced the pH change, ranging from 6 to 8.4 during cultivation. The 50 ml CO2 supplementation at pH 6 resulted in the highest biomass production (0.418±0.022 mg/l), lipid production (46%), and total carotenoids (21%). Under this condition, the culture color changed gradually, correlated with the change of chlorophylls and carotenoid contents. Statistical analysis (α = 0.05) confirmed that CO2 supplementations significantly affected biomass, lipid, and carotenoid productions. In conclusion, Coelastrella sp. strain Saripa demonstrated potentials in reducing atmospheric CO2, providing the biomass as lipid and carotenoid bioresources. Keywords: Coelastrella sp., microalgae, CO2 gas supplementation
Studi In Silico Sekuen NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) pada Jeruk yang Terkait dengan Resistensi Huanglongbing Nugroho, Kristianto; Santoso, Tri Joko; Kosmiatin, Mia; Sukma, Dewi; Purwito, Agus
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i2.25

Abstract

The evaluation of citrus resistance to Huanglongbing (HLB) disease is still challenging due to the incapability of the bacteria to be cultured purely in artificial medium, the complexity of inoculation methods, and the long duration required for phenotypic observation. Thus, the use of molecular markers is one of the alternatives to solve this problem. The focuses of this study were to perform in silico analysis of the nucleotide variations in NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) gene sequence among several citrus genotypes whose resistance information to HLB have been known previously and to employ phylogenetic analysis among them. The NPR1 gene sequences from 20 genotypes which consisted of 14 citrus and six relative genotypes were collected in silico from Citrus Genome Database and analyzed using multiple sequence alignment program. A total of six interesting SNPs that could distinguish between susceptible and resistant citrus genotypes were detected in this study. As many as five SNPs were non-synonymous, while only one synonymous SNP that did not cause the amino acid change was identified in this study. The phylogenetic analysis also revealed the separation between susceptible and tolerant/resistant citrus genotypes in two main clusters. The SNPs found in this study are expected to be useful for designing new functional markers as a selection tool in future studies. Keywords: in silico, molecular marker, phylogenetic analysis, selection tool, SNP
Aktivitas Antibakteri In Vitro dan Sitotoksisitas Pendahuluan dari Kombucha Kunyit Putih (Curcuma zedoaria) Siregar, Tobias Hezkel; Muttaqin, Mafrikhul; Syaefudin
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

White turmeric (Curcuma zedoaria) contains bioactive compounds with antibacterial and cytotoxic potential; however, the quantity of these compounds and their biological activity remain relatively low. Fermentation can enhance these properties by promoting microbial bioconversion. This study evaluated the total flavonoid content as well as antibacterial and cytotoxic activities of C. zedoaria rhizomes fermented using a Symbiotic Culture of Bacteria and Yeast (SCOBY). Antibacterial activity was evaluated against Escherichia coli and Staphylococcus aureus using the disk diffusion method, while cytotoxic activity was analyzed using the Brine Shrimp Lethality Test (BSLT). The results showed that fermentation increased the total flavonoid content by 64% and substantially improved antibacterial performance, producing inhibition zones of 13.7±0.7 mm for E. coli and 12.8±0.4 mm for S. aureus, more than double those of the non-fermented white turmeric. Cytotoxic activity also increased, as indicated by a lower LC50 value (145.3 µg/ml) compared to the non-fermented white turmeric (1,381.8 µg/ml). Overall, the findings demonstrate that fermentation significantly enhances the antibacterial and cytotoxic activities of white turmeric. Keywords:                 bioactivity enhancement, fermentation, functional beverage, SCOBY, white turmeric

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