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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 20 Documents
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Search results for , issue "Vol. 11 No. 1 (2024)" : 20 Documents clear
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
SARS-CoV-2 VARIANTS GENOME ANALYSIS OF INDONESIAN ISOLATES AND THEIR RESPONSES TO AVAILABLE VACCINES Audah, Kholis Abdurachim; Shaikho, Nasser Mohamed Ghassan Mohamed Adnan; Yurico, Dyana; Jeremy, Michael; Anisa, Amalda Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5547

Abstract

SARS-CoV-2 is a virus that initially appeared in Wuhan, China, at the end of 2019. In Indonesia, multiple variants of SARS-CoV-2 have been identified, as well as various local variants that are not yet considered to be ‘variants of concern’. Therefore, this investigation is intended to understand the prevalence and epidemiology of the virus, along with detecting the mutations that occur in genes associated with whole-genome-sequences (WGS) isolated in Indonesia. Analyses were performed to investigate SARS-CoV-2 prevalence in Indonesia using data obtained from GISAID.org. Whole-genome sequencing was performed on random samples taken from GISAID.org utilizing the BLAST tool from NCBI. The variants identified in Indonesia are alpha, beta and delta variants, as well as local variants B.1.470 and B.1.466.2. In total there are 9,260 isolated genomes collected in GISAID were located in Indonesia. Using BLAST, the variants were compared with the Wild-Type from Wuhan NC.045512.2. Multiple mutations were observed in the samples. The results from whole-genome sequencing of variants isolated in Indonesia have found that multiple mutations have occurred in genes of the SARS-CoV-2 virus and it caused alterations in the characteristics of the virus and may affect vaccine efficacy.
OPTIMIZATION OF METHANE PRODUCTION FROM MIXED SUBSTRATES OF COW FAECES AND CARICA SEEDS USING RESPOND SURFACE METHODOLOGY Sutaryo, Sutaryo; Susanto, Setio; Purnomoadi, Agung; Abeng, Doni; Purwasih, Rita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5554

Abstract

Anaerobic digestion (AD) of dairy cow feces (DCF) has low methane production per ton of waste. A strategy to overcome this drawback is to co-digest DCF and carica seed (CS). Currently, CS is still a waste from the candied carica in the syrup industry and is often just thrown away into the environment. This research aims to evaluate the optimal level of combination for methane production from DCF with co-substrates of germinated (CGM) and non-germinated (CNG) CS meal using Respond Surface Methodology (RSM). This research uses a completely randomized design with a factorial pattern consisting of the first factor being CGM and CNG, and the second factor being the combined level of DCF and CS (CGM or CNG. The result showed that utilization of CS can increase significantly (p<0.05) methane production of the final substrate compared to the control (digester treating DCF only). Utilization of CGM as co-substrate with DCF can also increase methane production (p<0.05) compared to CNG. All parameters in the liquid phase were in the normal range for AD. Based on the research results, the optimum point with a desirability value close to 1 was achieved at a ratio of CGM10 and DCF90. CS was proven can be used as a co-substrate with DCF to increase methane production of the final substrate and germination can be used as a method to increase the methane yield of CS. 
DIVERSITY AND DENSITY OF MEGABENTHOS IN CORAL REEF ECOSYSTEMS IN KERA ISLAND WATERS, KUPANG DISTRICT, INDONESIA Kangkan, Alexander L.; Sine, Kiik G.; Boik, Rini I.
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5597

Abstract

Kera Island belongs to the small islands of the East Nusa Tenggara with the potential conservation of coral reefs and various associated megabenthos organisms. This research aims to a) determine the diversity and density of megabenthos in coral reef ecosystems in Kera Island Waters, b) investigate the grouping between variables from environmental characteristics of megabenthos in coral reef ecosystems in Kera Island Waters, and c) observe the relationship between the diversity and density of megabenthos with the percentage of coral cover in Kera Island Waters. The research method used was a survey with direct measurements in the field. Megabenthos data was taken using the Benthos Belt Transect technique, while the coral cover was taken using the Underwater Photo Transect technique and analyzed using CPCe 4.1 software. The research data were then analyzed for density and percentage of megabenthos, diversity index value, (H’) Shannon, coral cover, organic matter, carbonate content, principal component analysis, and correlation. The study's results regarding the diversity index value of megabenthos in Kera Island Waters ranged from 0.796 to 1.631. The similarity of environmental parameters makes two main components. The first includes current velocity, organic content, dissolved oxygen, and depth; the second are carbonate content, TDS, temperature, and salinity characterize the second main component. The average organic matter was 8.32%, while the carbonate content was 50.09%. The coral reefs and megabenthos density had a robust relationship, with a correlation coefficient of 0.93.
TEMPERATURE EFFECTS ON THE STABILITY OF MYCOBACTERIUM TUBERCULO-SIS SHIKIMATE KINASE (MtSK): STRATEGIES FOR SECURE TRANSPORT Wibowo, Aji; Komariyah, Tinta; Prabandari, Erwahyuni Endang; Ariyani, Titin; Siska, Eka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5751

Abstract

Mycobacterium tuberculosis Shikimate Kinase (MtSK) has a crucial role in the shikimic pathway, which is essential for this bacteria but is absent in humans, making it a potential target for novel anti-tuberculosis drugs. This study used enzyme-coupled fluorescence to examine the stability of MtSK stored in 50% glycerol at -30 ℃, 4 ℃, and ±28 ℃ for six days. Results showed stable enzyme activity values (α=0.05) at all temperatures. This research underscores that MtSK’s stability depends on its molecular properties, including GC content, hydrophobic residues, Mg2+ binding, and intra-helical salt bridge. Despite some activity decline over time due to glycerol-induced aggregation, MtSK can be safely transported at ±28 ℃ for up to six days without special cooling compartment. Understanding MtSK stability ensures its active conformation remains consistent, reducing off-target effects on drug design and enhancing drug efficacy. This insight ultimately leads to high-quality and commercially viable tuberculosis treatment development. Future research should explore MtSK stability at higher temperatures and assess the optimal glycerol content for cryopreservation.
LEYDIG CELL COUNT IS INCREASES IN OLD WISTAR RATS (RATTUS NORVEGICUS) BY ANTIOXIDANT ETHANOL EXTRACT OF MORINGA OLEIFERA (EEMO) Widiastini, Luh Putu; Karuniadi, I Gusti Agung Manik
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5807

Abstract

Oxidative stress is the result of an imbalance between antioxidant production and the production of reactive oxygen species (ROS). Leydig cells have a high content of PUFAs in their cell membrane, making it easy to bind ROS. Most natural antioxidants are found in plants, including Moringa. In this work, aged Wistar rats' Leydig cells will be used to test the antioxidant activity of an ethanol extract from the leaves of Moringa oleivera (Rattus norvegicus). The study involved the division of 36 elderly rats, who were between the ages of 18 and 19 months, into two groups. For thirty days, the control group received the same quantity of 0.5% CMC every day, whereas the treatment group received 50 mg/kgBW/0.5 mL of Moringa leaf ethanol extract daily. The rats were healthy and free of physical impairments. The Independent Samples T-Test is used to assess the data in order to find any variations between the treatment and control groups. The significant difference (p < 0.00) in Leydig cell counts between the extract-treated group and the control group suggests that the ethanol extract of Moringa leaves can have a major effect on the number of Leydig cells in Old Wistar rats (Rattus Norvegicus).
ANTIFUNGAL ACTIVITY OF N-HEXANE EXTRACT FROM Chaetoceros calcitrans AGAINST Candida sp. Rachmawati, Firdha; Naully, Patricia Gita; Kania, Prina Puspa; Pasha, Delia Ayu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6202

Abstract

Skin infections caused by Candida albicans and Candida krusei pose a serious health issue. One major concern regarding these infections is the resistance to antifungal drugs, highlighting the need for natural antifungals. Chaetoceros calcitrans, a microalgae, is known to contain natural antimicrobial compounds. This study aims to evaluate the antifungal potential of C. calcitrans n-hexane extract against both pathogens. The antifungal activity was tested using the diffusion method. The results indicated that the extract at a concentration of 100 mg mL⁻¹ inhibited the growth of C. albicans and C. krusei, showing the highest inhibition zones of 10.3 ± 0.9 mm and 9 ± 1.4 mm, respectively. GC-MS analysis revealed that the C. calcitrans extract contains antifungal compounds, including 2-Butyl-1-hexyloctahydro-1H-indene, at a concentration of 30.72%. Therefore, it can be concluded that C. calcitrans extract possesses antifungal activity and has potential as a drug candidate for fungal skin infections.
THE CURRENT STRATEGIES, RECENT PROGRESS AND REMAINING CHALLENGES FOR DEVELOPING MRNA VIRAL VACCINE Irawan, Priscilla Felicia Apriliani; Bela, Budiman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6422

Abstract

The mRNA expression system has revolutionised biotechnology, notably in viral mRNA vaccine development, cancer immunotherapy, and gene therapy. However, recent safety concerns regarding the COVID-19 mRNA vaccine have emerged, particularly regarding its rare adverse effects and its possible connection to cancer. This review explains several approaches used in developing viral mRNA vaccines, the past obstacles solved in generating the current COVID-19 mRNA vaccine, and finally the current advancements and ongoing challenges in the viral mRNA vaccine field. We particularly focus on strategies and methods to improve the safety and translation efficiency of the mRNA vaccine, such as enhancing the vaccine’s transfection specificity to targeted dendritic cells (DC) and using viral IRES or self-amplifying mRNA format to improve mRNA translation efficiency.
BIOACTIVE METABOLITES OF Lactiplantibacillus plantarum ISOLATED FROM Sonchus arvensis AS AN ANTIMICROBIAL AGENT Syaputri, Yolani; Nurhidayat, Fahmi; Wulandari, Indri; Rahayu, Sri Rejeki
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6488

Abstract

Sonchus arvensis is a plant widely found in Southeast Asia and often used in traditional medicine. Lactiplantibacillus plantarum is a bacteria isolated from S. arvensis and categorized as qualified presumption of safety by the European Food Safety Authority and the US Food and Drug Administration. Therefore, this study aimed to determine the bioactive metabolites of Lpb. plantarum as antimicrobial agents. Lactic acid bacteria (LAB) were isolated using the serial dilution method, followed by isolation and amplification of the DNA through a 16S rRNA universal primer. Antimicrobial activity was screened using the well-diffusion method. Plantaricin gene identification was performed using PCR and the determination of lactic acid content was conducted using Spectrophotometric. In addition, the titration method was used to measure and determine the hydrogen peroxide. The results showed that Lpb. plantarum had higher inhibition toward pathogen bacteria than Lc. lactis. Lpb. plantarum had the largest inhibition zone against B. subtillis, followed by S. aureus and E. coli, respectively. It was discovered that Lpb. plantarum precipitated with ammonium sulfate had a greater protein content and antibacterial activity. Furthermore, Lpb. plantarum, which encodes plnA and plnEF, produced lactic acid and hydrogen peroxide at concentrations of 3.0158±0.2774 mg/mL and 0.195±0.04 mg/mL, respectively.
MORPHOLOGICAL INDICES: DISTINGUISHING SINGLE AND TRIPLETS-BEARING BOER AND ETAWA GRADE GOATS IN LOMBOK BARAT Fitriyah, Abyadul; Mariani, Yuni; Kartika, Ni Made Andry; Fajri, Nefi Andriana; Alimuddin; Harmayani, Ria
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.7120

Abstract

This study aimed to identify female goats with the potential to give birth to triplets by examining morphological characteristics correlated with FSH and LH hormone levels in the blood. It included four triplet-bearing Boer goats and four triplet-bearing Etawah grade goats, each with 12 kids, and four single-bearing Boer goats and four single-bearing Etawah grade goats, each with four kids. Morphological indices of body sizes, including weight, height slope, length index, width slope, depth index, foreleg length, and hormone levels, were observed. A t-test and descriptive analysis were conducted. The average body size of Boer goats was larger than Etawah grade goats, but there were no significant morphological differences between triplet-bearing Boer and Etawah grade goats. However, single-bearing Boer goats had a significantly higher height slope than single-bearing Etawah grade goats. FSH levels significantly increased in the blood of triplet-bearing Boer goats compared to single-bearing Boer goats. Morphological indices and hormone levels can help select goats likely to give birth to triplets.

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