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I Putu Cahyadi Putra
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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.2024.v16.i05.p01
Core Subject : Health, Science,
Immunology & microbiology Public Health Veterinary
FOCUS The journal focused on Veterinary Medicine, Animal Sciences, and Health Studies with various developments. SCOPE Zoonoses, Public Health, One Health, Epidemiology, Reproduction, Virology, Bacteriology, Parasitology, Mycology, Internal Medicine, Surgery, Orthopedics, Vaccines, Genetics, Molecular Biology, Physiology, Nutrition and Dietetics, Feed Science, Meat Science, Animal Welfare, Animal Behavior, Endocrinology and Metabolism, Animal Care, Veterinary Education, Livestock Management and Production, Poultry, Horse Science, Cattle Science, Small Ruminants, Pig Science, Aquaculture, Fish Diseases, Fisheries Science and Fish Nutrition, Zoo Animal Management, Zoo Animal Disease Studies, Wildlife Diseases, Conservation Science, and other related studies.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 394 Documents
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THE EFFECTS OF TRANSFORMATION DURATION ON THE QUANTITY OF SWINE PATHOGENIC ESCHERICHIA COLI ISOLATE Kalyana Lionita; I Gusti Ngurah Kade Mahardika; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p04

Abstract

DNA transformation generally still utilizes commercial Escherichia coli bacteria, and the use of virulent pathogenic bacteria has never been reported. Utilizing virulent pathogenic bacteria can provide an advantage, as the product can be used as a vaccine against the genetic material in the plasmid and against the pathogenic bacteria itself. This study aims to determine how the transformation duration affects the number of swine Escherichia coli pathogenic isolates colonies. The samples of this study are swine Escherichia coli pathogenic isolates BPOS4 and E. coli BL21 (DE3) which were made into competent cells by calcium chloride heat shock method and then transformed with the different transformation of times using plasmid pGEX-2T+ASF A224L. The results showed that E. coli BL21 (DE3) and swine Escherichia coli pathogenic isolates BPOS4 had no significant effect on the quantity of colonies after transformation. Although statistically the effect was not significant, E. coli BL21(DE3) gave a higher colony count with an average of 62,4×105 CFU/100µL compared to E. coli BPOS4 of 33,45×105 CFU/100µL. The transformation time has no significant effect on the number of bacterial colonies after transformation, however, the best transformation time is shown by the treatment of 60 seconds with an average colony of 86,5×105 CFU/100µL. It can be concluded that the colonies of E. coli BL21 (DE3) and E. coli BPOS4 transformed with plasmid pGEX-2T+ASF A224L can grow optimally in 60 seconds. Further research can be conducted, such as developing Escherichia coli vaccines using pathogenic strains.
TREATMENT OF STAPHYLOCOCCUS DISEASE IN PIGS USING A COMBINATION OF PROCABEN LA, SULPIDON, AND BIOSAN Paulus Oktavianus Pyo; Arkadius Suban Keray; Matheus Mbele Dede
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p05

Abstract

Traditional livestock management systems that lack proper sanitation can facilitate the emergence of infectious diseases, including those caused by Staphylococcus bacterial infections. Staphylococcus spp. can affect the respiratory tract and, if left untreated, may lead to livestock mortality, resulting in significant economic losses. This case study aims to document the treatment of Staphylococcus infections in pigs using Procaben LA, Sulpidon, and Biosan in Kupang City. The subject of this case was a four-month-old female pig with black coloration and an approximate body weight of 50 kg, originating from Kupang City. Observed clinical signs included reduced appetite, lethargy, pale pink conjunctiva and gingiva, and a capillary refill time (CRT) exceeding two seconds. Clinical examinations comprised assessments of rectal temperature, heart rate, respiratory rate, and systemic organ function. Hematological and bacterial identification tests were routinely conducted at the UPT Veterinary Laboratory, Provincial Livestock Service of East Nusa Tenggara. Bacterial culture on Mannitol Salt Agar (MSA) medium yielded colonies suspected to be Staphylococcus spp., characterized by round, elongated gray colonies with rough surfaces and clustered cocci morphology. Based on anamnesis, clinical examination, laboratory testing, and bacterial identification, the pig was diagnosed with a Staphylococcus infection. The treatment regimen included Procaben LA (4 mL), Sulpidon (5 mL), and Biosan (5 mL), all administered via intramuscular injection (IM). The therapy yielded positive outcomes, with notable improvements in the pig’s condition, restoration of appetite, and normalization of body temperature.
ANTIBODI TITER LEVELS IN LOCAL DOGS FOLLOWING RABIES VACCINATION IN PENARUNGAN VILLAGE, MENGWI DISTRICT, BADUNG REGENCY Petra Rahmitha Sava Setyanto; Ida Bagus Kade Suardana; I Nyoman Suartha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p06

Abstract

Rabies is caused by a virus from the genus Lyssavirus, which belongs to the Rhabdovirus family. In Badung Regency, 13 cases of rabies in dogs were confirmed in 2015, with 8 positive cases reported in Mengwi District. This study aims to measure the antibody titer against the rabies virus in local dogs that had been vaccinated in Penarungan Village, Mengwi District, Badung Regency, seven months after vaccination. The method used in this study was antibody titer testing through the Enzyme-linked Immunosorbent Assay (ELISA). Samples were selected based on inclusion criteria and were collected from Banjar Blungbang, Banjar Cemenggon, Banjar Guming, Banjar Umopoh, and Banjar Bantas. A total of 16 blood samples from local dogs were collected using the venipuncture technique. The results of the study using the ELISA test showed that 12 samples were seropositive, with an average titer value of 0.9 IU/mL, while 4 samples were seronegative, with an average titer value of 0.4 IU/mL. The percentage of seropositive antibody titers in local dogs in Penarungan Village seven months after rabies vaccination was 75%, while the percentage of seronegative antibody titers was 25%. Therefore, booster rabies vaccinations are necessary for dogs with seronegative antibody titers.
PREVALENCE COMPARISON OF NEWCASTLE DISEASE VIRUS CASES AT CHICKEN FARMS IN BLAHBATUH AND PAYANGAN SUBDISTRICTS, GIANYAR DISTRICT Ingrid Laurentina; Gusti Ayu Yuniati Kencana; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p07

Abstract

Newcastle Disease (ND) is a common poultry disease in Indonesia, caused by Avian Paramyxovirus serotype 1 (APMV-1). This disease is endemic and occurs throughout the year affecting various types of poultry, including commercial and backyard chickens. This study aims to compare the prevalence of the ND virus in poultry farms in Blahbatuh and Payangan Districts, Gianyar Regency. This study employed an observational method with a cross-sectional approach. Chickens showing clinical symptoms were subjected to necropsy to collect tissue samples that exhibited pathological changes. These samples were inoculated into Specific Pathogen-Free (SPF) embryonated chicken eggs for ND virus growth. After incubation, the allantoic fluid was harvested and tested using HA and HI. A positive HA test result was indicated by the formation of sand like sediment, while a positive HI test result showed a 1% erythrocyte deposit forming a dot at the bottom of the microplate well. The study results indicated that 9 out of 32 chickens (28%) tested positive for ND. Chickens sampled in Payangan Subdistrict were found to have a 1.36 times higher risk of infection compared to those sampled in Blahbatuh subdistrict. Increasing farmers awareness of the importance of regular ND vaccination and improving biosecurity like isolation cage systems is crucial to preventing the spread of the disease.
PRESERVATION OF CANINE FRONT AND HIND LIMB SPECIMENS USING PLASTINATION TECHNIQUE: IMPREGNATION PHASE IN A PASSIVE VACUUM CHAMBER Luh Gde Sri Surya Heryani; I Nengah Wandia; I Ketut Suatha; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p08

Abstract

The plastination technique is a preservation method that involves the infusion of polymer materials into biological specimens to maintain their structural integrity, prevent decay, and ensure long-term durability. This technique is widely regarded as an effective approach for preserving organs and is particularly valuable for anatomical education. The objective of this study is to evaluate the qualitative characteristics—specifically texture, color, and odor—of plastinated canine front and hind limb specimens during the impregnation phase, conducted within a passive vacuum chamber. Additionally, this research aims to propose recommendations for refining plastination techniques to enhance the quality of preserved organ specimens. The study employs a custom-designed apparatus and readily available generic chemicals to perform the plastination process. The plastination procedure consists of four key stages, with the impregnation phase being carried out in a vacuum chamber utilizing a passive vacuum system. The resulting plastinated specimens were assessed for flexibility, color, and odor. The findings revealed that the plastinated organs exhibited a rigid texture, a pale coloration, and a mild odor. The anticipated outcome of this research is to provide actionable recommendations for improving plastination methods, thereby enhancing the quality of plastinated organ products for use in veterinary anatomy education.
ANTIBIOTIC SENSITIVITY OF ALPHA-HEMOLYTIC MUCOID ESCHERICHIA COLI FROM DIARRHEIC BALI CATTLE TO KANAMYCIN, ERYTHROMYCIN, AND AMPICILLIN Beatrice Cecilia Rebecca Situmorang; I Gusti Ketut Suarjana; I Wayan Sudira
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p02

Abstract

Diarrhea in Bali cattle caused by Escherichia coli alpha-hemolytic mucoid strain can lead to significant economic losses due to reduced livestock productivity. To address this issue, this study evaluated the antibiotic sensitivity patterns of E. coli isolates to kanamycin, erythromycin, and ampicillin. A total of 11 E. coli isolates were obtained from the feces of diarrheic Bali cattle and tested for antibiotic susceptibility using the Kirby-Bauer disk diffusion method on Mueller-Hinton agar. The inhibition zone diameters were measured and interpreted according to Clinical and Laboratory Standards Institute (CLSI) guidelines, categorizing the isolates as sensitive, intermediate, or resistant. The results revealed varying susceptibility patterns among the isolates. For kanamycin, 81.8% of the isolates were susceptible, 18.2% showed intermediate susceptibility, and none were resistant (0%). In contrast, all isolates (100%) exhibited complete resistance to both erythromycin and ampicillin, with no susceptibility or intermediate resistance observed (0% each). These findings underscore the critical need for prudent antibiotic selection in treating E. coli-induced diarrhea in Bali cattle. Additionally, this study highlights the urgency of further research into resistance mechanisms and the development of sustainable infection control strategies.
ERYTHROCYTE PROFILE OF GOLDFISH FROM SELLER IN DENPASAR, BALI Giovanni Owen Girsang; Sri Kayati Widyastuti; I Made Sukada
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p06

Abstract

Goldfish (Carassius spp.) is one of the most popular ornamental freshwater fish in Indonesia, originating from China and domesticated since 1860 with various color variants and unique body shapes resulting from selective breeding. This study aimed to analyze the erythrocyte profile of goldfish sold in Denpasar, Bali, as a baseline for health status assessment and early disease detection. This observational descriptive study examined blood samples from 10 healthy goldfish (5 males and 5 females) with lengths of 7-10 cm. Blood collection was performed at the Animal Hospital of the Faculty of Veterinary Medicine, Udayana University, using 1 ml syringes with 26G needles following anesthesia with Koi Anesthesia®. Complete blood count analysis was conducted using an Auto Hematology Analyzer Rayto RT-7600 for Vet, while blood smears were prepared using Diff-Quik staining for morphological examination. The results showed mean hemoglobin (Hb) values of 3.25 g/dL (range: 1.9-6.4 g/dL), mean hematocrit (Hct) of 4.55% (range: 0-9.1%), and mean erythrocyte count (RBC) of 0.15 × 10⁶/μL (range: 0.01-0.88 × 10⁶/μL). Erythrocyte indices revealed mean MCV of 96.85 fL (range: 81.3-112.4 fL), mean MCH of 88.35 pg (range: 52.6-124.1 pg), and mean MCHC of 80.1 g/dL (range: 48.3-151.4 g/dL). It can be concluded that the erythrocyte profile of goldfish in Denpasar shows considerable individual variation, which may reflect differences in genetic factors, environmental conditions, or handling practices during the study. It is recommended to improve blood collection techniques to minimize cell damage and contamination, potentially through additional training with experienced practitioners and protocol refinement to ensure more valid results that accurately reflect the physiological condition of goldfish.
COMPARISON OF ADJUVANT EFFICACY IN STREPTOCOCCUS SUIS VACCINATION IN MICE: SECONDARY ANTIBODY TITER ANALYSIS USING ANTIGEN SUPERNATANT BASED ELISA I Made Yudhara Teresnanda; I Nengah Kerta Besung; Putu Devi Jayanti; I Gusti Ngurah Kade Mahardika; Ni Ketut Suwiti
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p07

Abstract

Streptococcus suis is a Gram-positive zoonotic bacterium that causes various diseases in pigs, such as meningitis, arthritis, and septicemia, which affect animal health and the economic viability of the livestock industry. Vaccination, supported by appropriate adjuvants, is an effective control strategy to enhance immune responses. This study aimed to evaluate secondary antibody responses in mice vaccinated with S. suis antigen using two different adjuvants, Montanide™ ISA 201 VG and Montanide™ Gel 01, and to analyze the dynamics of antibody titer increase. Eighteen mice were divided into three treatment groups: a control group receiving adjuvant without antigen, a vaccine group with Montanide™ ISA 201 VG, and a vaccine group with Montanide™ Gel 01. Vaccination was administered twice, and blood samples were collected weekly for four weeks post-vaccination. Antibody titers specific to S. suis supernatant antigen were measured using the ELISA method. Data were analyzed using ANOVA, Least Significant Difference (LSD) test, and regression analysis. The results showed that both adjuvants significantly increased antibody titers compared to the control (p<0.05), with a progressive increase in titer levels. Montanide™ ISA 201 VG provided sustained stimulation through a depot effect, while Montanide™ Gel 01 elicited an effective immune activation with minimal local reactions. In conclusion, the combination of S. suis antigen with both adjuvants significantly enhanced humoral immune responses, supporting the potential of both adjuvants to improve S. suis vaccine efficacy. Further research is needed to determine the optimal dose and duration of post-vaccination immune protection.
HISTOPATHOLOGICAL ANALYSIS OF INCISION WOUND HEALING IN RATS GIVEN PLATELET RICH PLASMA DROPS FROM PIG BLOOD Anak Agung Ngurah Hery Brian Priantara; I Wayan Wirata; I Ketut Berata; I Gusti Ngurah Sudisma; I Wayan Gorda Sudisma; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p09

Abstract

Platelet Rich Plasma (PRP) is platelet-rich plasma derived from whole blood. Platelet Rich Plasma (PRP) contains growth factors such as transforming growth factor-beta (TGF-β), insulin like growth factor (IGF-I), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and epidermal growth factor (EGF) which play a role in accelerating wound healing. This study aims to determine the histopathological analysis of incision wound healing of white rats given pig blood PRP drops. This study used white rats of male sex with the age of 2 - 2.5 months with a body weight of 200 - 300 g. The rats used in this study were 27 rats divided into two groups. The 27 rats used were divided into 3 treatment groups, namely P0 (negative control, given 0.9% NaCl solution), P1 (positive control, given Bioplacenton), P2 (given PRP drops). On days 1, 5, and 11, biopsies were taken for histopathological examination. Histopathological examination was performed including four indicators: inflammatory cell infiltration, angiogenesis, fibroblasts, and collagen density. Data were then analyzed using Kruskal-Wallis and if there was a significant difference (P<0.05), it would be followed by the Mann Whitney test. From the results of the study on the histopathology of white rat incision wounds given pig blood PRP drops showed an increase in development. On the first day, inflammatory cell infiltration and angiogenesis increased, but decreased on days 5 and 11. Fibroblasts were seen on the first day then decreased until day 11. Collagen on day 1 began to be seen until on day 11 the density of collagen was very tight. It is necessary to make observations in a more detailed period of time to find out more clearly about the effect of PRP drops on angiogenesis.
THE EFFECT OF MENIRAN EXTRACT AS AN IMUNOSTIMULATOR ON THE INCREASE OF NEWCASTLE DISEASE ANTIBODY TITER IN BROILERS Muhammad Evan Malik; Ida Bagus Kade Suardana; Samsuri; Ni Luh Eka Setiasih; I Made Kardena; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p11

Abstract

Meniran is known as an herbal plant with potential as a natural immunostimulator due to its content of flavonoids, saponins, and tannins. These compounds help boost the immune response by stimulating cytokine production, accelerating immune cell activation, and increasing antibody production. Newcastle Disease (ND) is an infectious disease that frequently affects broilers and causes significant economic losses. Vaccination is the primary method for preventing ND; however, its effectiveness can vary depending on the immune response of the chickens. This study aims to evaluate the effect of meniran extract as an immunostimulant on increasing Newcastle Disease antibody titers in booster-vaccinated broilers. This study used a Completely Randomized Design with 30 Lohman MB 202 broilers divided into three treatment groups. The negative control group (K-) received neither vaccination nor meniran extract, the positive control group (K+) received only the ND La Sota booster vaccine, and the treatment group (P) received both the ND La Sota booster vaccine and meniran extract for 14 days before vaccination. Antibody titers were measured using the Hemagglutination Inhibition (HI) test on days 17, 25, and 32. Data were analyzed using ANOVA and regression analysis. The results showed that the administration of meniran extract significantly increased antibody titers (P<0.05), with mean values of 2.7, 4.1, and 6.0 (HI log 2), and an overall mean of 4.27. Regression analysis indicated that the treatment group (P) experienced a higher increase in antibody titers compared to the control groups, with the peak immune response occurring in the third week post-vaccination. The conclusion of this study is that meniran extract can act as a natural immunostimulant that enhances the effectiveness of the ND vaccine in broiler chickens. Therefore, further research with different doses and administration durations is needed to optimize its benefits.

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