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JURNAL SAINS DAN TEKNOLOGI INDONESIA

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Search results for , issue "Vol. 12 No. 1 (2010)" : 12 Documents clear

APLIKASI TEKNOLOGI LASERPUNKTUR DALAM MENINGKATKAN LIBIDO PEJANTAN DOMBA GARUT (Ovis Aries) herdis, herdis
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Libido can be measured by the length of time needed by the ram from teasing ewes to ejaculation. Low libido is one inefficient factor in reproductive process of sheep. The research was carried out to assess the application of laserpuncturetechnology for increasing libido of Garut rams. Results of the research showed that from three ejaculations observed, the ram which was treated with laserpuncture was more responsive in its libido than control. At the first ejaculation, the ram which was treated with laserpuncture needed 20.67 seconds and more responsive than control 35,40 seconds. At the second ejaculation, the laserpuncture treated needed 85 seconds to responsif than control 129,2 seconds. At the third ejaculation, the laserpuncture treated needed 198,6 seconds to responsif than control 209 seconds. In conclusion, laserpuncture at six acupoints in Garut rams can improve libido.

MEMECAH DORMANSI RIMPANG TEMULAWAK (Curcuma xanthorrhiza ROXB) MENGGUNAKAN LARUTAN ATONIK DAN STIMULASI PERAKARAN DENGAN APLIKASI AUKSIN Djamhari, Sudaryanto
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Temulawak is a plant propagated vegetatively with rhizomes. Its rhizome undergoes a dormancy phase in dry season and this phase will break up in wet season. The objective of this study is to know the dormancy breaking by soaking the temulawak rhizome in stimulating biology liquid (0, 2, 4, 6 ml/l) and commercial auxin liquid (0, 10, 20, mg/l). The result showed that both liquid could stimulate the dormancy breaking. However those treatments were not different with control (biology liquid 0 g/l and commercial auxin 0 mg/l ). The number of shooting rhizomes, total shoots, rooted rhizomes and total roots in all treatment were not significantly different from the control.

DEMINERALISASI DAN DEPROTEINASI KULIT UDANG SECARA KONTINYU PADA TAHAPAN EKSTRAKSI KITIN SECARA BIOLOGIS waltam, deden rosid; Hermansyah, Heri; Setyahadi, siswa
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Chitin extraction in industry has been conducted by chemical process. The process has been known as a harsh treatment that badly affected to chitin quality, equipment and the environment. Since the last decade biologically chitin extractionhas more attracted attention. The biologically chitin extraction was conducted by batch fermentation or subsequent-batch fermentation. Continous demineralization and deproteinization is a new inovation on biologically chitin production technology.This system promises as an alternative technology for overcoming problems of batch fermentation process and chemical process. The objectives of the experiment was to obtain the optimal condition for continous deminineralizationand deproteinization for producing chitin from Panaeus vannamei shrimp shells. Lactobacillus acidophilus FNCC 116 and Bacillus licheniformis F11.1 was used for demineralization and deproteination process respectively. The results showed that the best condition for continuous demineralization was 6,5% glucose feed, with 16 hours retention time. For continuous deproteinization, the best condition was with 12 hours retention time. The process could remove 92.95% ash and 91.40% protein. The chitin, ash, and protein content of chitin product was 96.69%, 1.44% and 1,76% respectively.

PENGARUH FOTOSINTESIS TERHADAP PERTUMBUHAN TANAMAN KENTANG (SOLANUM TUBEROSUM L.) DALAM LINGKUNGAN FOTOAUTOTROF SECARA INVITRO Pertamawati, Pertamawati
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

The growth responces of potato explant (var. Atlantic), under different temperatures (100C) between photoperiod and dark period air temperatures in culture room, in high intensity of light (7000 lux) and low intensity of light (3000 lux), cultured in 18 h light/dark cycle and incubated for 21 and 28 days wasobserved. The result shown that photoautotroph condition influence the explant growth to be planlet. After 28 days incubation the planlets growth better than in 21 days. Its because the photosynthesis process during 28 days incubation were more effective than in 21 days incubation. The dry weight per planlet, number of leaves, leaf area and dry weight per leaf were enhanced in photoautotroph condition with 7000 lux light intensity in all treatments. In 3000 lux light intensity, the high planlet with small diameter was performanced. The study indicates that the photoautotroph condition affect further growth of the in vitro potato planlet.

KUALITAS INDUK UDANG WINDU (Penaeus monodon) DI PERAIRAN PANTAI PANGANDARAN, SELATAN JAWA BARAT Sachoemar, Suhendar I; aliah, ratu siti
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jsti.v12i1.853

An evaluation of the quality of the Black Tiger Broodstock Shrimp (Penaeus monodon) in the southern coastal area of Pangandaran, West Java was conducted during period of Juny-October 2005. The evaluation was focused on the waterquality identification and the quality of the broodstock collected from the farmer sorround the Pangandaran Area. The result of the evaluation showed that the water quality of the coastal area of Pangandaran was good compare to the northern coastal area of West Java in which the organic and inorganic pollutantwas . This situation was affected on the quality of the broodstock. The identification result on the quality of broodstock using polymerase chain reaction (PCR) method shows that the broodstock shrimp of the Black Tiger Shrimp(Penaeus monodon) in the coastal area of Pangandaran is free from the White Spot Syndrome Virus (WSSV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV). From 99 broodstock identified, only 4 indicated by very light contaminated WSSV and 2 by IHHNV. This condition shows that in general the quality of the broodstock shrimp of the Black Tiger Shrimp (Penaeus monodon) in the coastal area of Pangandaran was excellent and these sources are potentialto be applied as broodstock sources for developing the Black Tiger Shrimp (Penaeus monodon) culture in the national level.

IDENTIFIKASI TERJADINYA TRANSFER GEN PADA TANAMAN Tjokrokusumo, Donowati S
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Identification of somatic hybridization is one of the important steps to cope with that fusion and migration of gene to be analyzed. Therefore, following fusion various approaches have been used to identify hybrid materials. Putative somatic hybridsmay be confirmed and characterized in a number of ways, depending upon the characteristics of the parental materials. Both characterization and identification methods are discussed in this paper.

PERILAKU DISOLUSI KETOPROFEN DAN INDOMETASIN FARNESIL TERSALUT GEL KITOSAN-GOM GUAR Sugita, Purwantiningsih; Srijanto, Bambang; arifin, budi; amelia, fithri; mubarok, mahdi
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Chitosan, a modification of shrimp-shell waste, has been utilized as microcapsule. However, it’s fragile gel property needs to be strengthened by adding glutaraldehyde (glu) and natural hydrocolloid guar gum (gg). This research’s purposes were to study dissolution behaviour of ketoprofen and infar through optimum chitosan-guar gum microcapsule. Into 228.6 mL of 1.75% (w/v) chitosan solution in 1% (v/v) acetic acid,38.1 mL of gg solution was added with concentration variation of 0.35, 0.55, and 0.75% (w/v) for ketoprofen microcapsules and 0.05, 0.19, and 0.33% (w/v) for infar microcapsules, and stirred with magnetic stirrer until homogenous. Afterwards, 7.62mL of glu was added slowly under stirring, with concentrations varied: 3, 3.5, and 4% (v/v) for ketoprofen microcapsules, and 4, 4.5, and 5% (v/v) for infar microcapsules. All mixtures were shaked for 20 minutes for homogenization. All mixtures wereshaked for 20 minutes for homogenization. Into each microcapsule mixture for ketoprofen, a solution of 2 g of ketoprofen in 250 mL of 96% ethanol was added, whereas solution of 100 mg of in 250 mL of 96% ethanol was added into each microcapsule mixture for infar. Every mixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Everymixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Conversion of suspension into fine powders/granules (microcapsules) was done by using spray dryer. The data of [gg], [glu], and medicine’s content from each microcapsule were treated with Minitab 14 software to obtain optimum [gg] and [glu] for microencapsulation. The dissolution behaviour of optimum ketoprofen and infar microcapsules were investigated. The result of optimization by using Minitab Release 14 software showed that among the microcapsule compositions of [gg] and [glu] were 0.35% (w/v) and 3.75% (v/v), respectively, optimum to coat ketoprofen, whereas [gg] and [glu] of 0.05% (w/v) and4.00% (v/v), respectively, optimum to coat infar, at constant chitosan concentration (1.75% [w/v]). In vitro dissolution profile showed that chitosan-guar gum gel microcapsule was more resistant in intestinal pH condition (rather basic) compared with that in gastric pH (very acidic).

UJI IN VITRO EKSTRAK AIR DAN ETANOL DARI BUAH ASAM GELUGUR, RIMPANG LENGKUAS, DAN KENCUR SEBAGAI INHIBITOR AKTIVITAS LIPASE PANKREAS Iswantini, Dyah; Darusman, Latifah K.; Fitriyani, Ana
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Asam gelugur fruits of Garcinia often used to reduce body weight. Lengkuas and kencur are traditional herbal that potential for antiobesity because they could reduce the level of phospholipids, triglycerides, and cholesterol. The aim of the research was to evaluate the potencies of these herbal as antiobesity by measurement of their water and ethanol extracts capabilities as in vitro inhibitor of pancreatic lipase activity. The water and ethanol extracts of asam gelugur fruits contained saponins and alkaliods, respectively. The water extract of lengkuas rhizomes contained alkaloids, flavonoids, saponins, and quinones, while the ethanol extract contained alkaloids, flavonoids, saponins, and steroids. The water extract of kencur rhizomes contained saponins and quinones, while the ethanolextract contained alkaloids, flavonoids, steroids, and quinones. The highest inhibitory effect of all extracts was obtained from the ethanol extract of asam gelugur fruits with value of 86.3% at 150 ppm. The highest inhibitory effect of lengkuas extracts was from the ethanol extract at 200 ppm (56.2%). The highestinhibitory effect of kencur was showed by the ethanol extract with the value 37.6% at 300 ppm. These values were higher than the inhibitory effect of the positive control (Xenical®) at 100 ppm (10.6%).

PEMANFAATAN COPEPODA Oithona sp SEBAGAI PAKAN HIDUP LARVA IKAN KERAPU aliah, ratu siti; yaniharto, dedy
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

The key success of producing grouper seed depends on the availability of high and complete nutritive live food (zooplankton) during larvae development stage. Rotiferand Artemia are common zooplankton that has been used in most of grouper hatchery. The cysts of Artemia is expensive because it’s still imported, therefore potential zooplankton from Indonesians waters should be developed to replace or to substitute Artemia. Copepod Oithona sp can be found easily in Indonesian marine waters. Oithona sp can be developed to adult stage within 7 days. One female Oithona can produce 8 to 22 nauplii with average 14,39 ± 3,62 nauplii. The larva ofhumpback grouper (Cromileptes altivelis) fed by Oithona sp showed a higher DHA content (0,30 % b/b) than those fed by copepod + Artemia (0,15 % b/b) and and Artemia only (0,03 % b/b). The EPA content in larva fed by Oithona also showed thehighest, 0,30 % b/b. However, improvement technique of monoculture Oithona should be carried out due to mass mortality and contamination by protozoan and rotifer.

PARTIAL DIALLEL CROSS ANALYSIS AMONG THREE BREEDS OF PIG FOR PRODUCTIVE AND REPRODUCTIVE TRAITS kurnianto, edy; Arifin, Mukh; Nugroho, Prasetyo
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Suatu penelitian telah dilakukan untuk mengevaluasi general combining ability (GCA) dan specific cobining ability (SCA) untuk sifat produksi dan reproduksi pada babi. Materi yang digunakan adalah catatan keturunan hasil persilangan tiga bangsa babi (Duroc, Landrace, Yorkshire) sebanyak 573 anak babi yang berasal dari 13 pejantan dan 65 induk. Formula yang digunakan untuk menganalisis parameter adalah Partial Diallel Cross Metode II dari Griffings. Parameters yang diamati pada anak adalah litter size, bobobt lahir, jumlah puting, pertambahan bobot badan (PBB) sebelum dan sesudah sapih dan bobot sapih. Hasil penelitian menunjukkan bahwa GCA Duroc lebih tinggi dibandingkan Yorksire and Landrace, yang nilai GCA untuk litter size, bobot lahir, jumlah puting, jumlah anak pada saat sapih, bobot sapih, PBB sebelum sapih, PBB setelah sapih dan bobot badan pada umur 42 hari masing-masing -0,18; -0,02; -0,52; -0,13; -0,21; -0,01; 0,01 dan 0,03. Persiilangan antara Duroc x Yorkshire menunjukkan SCA paling tinggi untuk bobot lahir (0.30), jumlah puting (3.60), bobot sapih (1.10) dan PBB sebelum sapih (0.04). Persilangan antara Yorkshire x Landrace menghasilkan SCA paling tinggi untuk litter size (2.47), jumlah anak waktu sapih (2.23), PBB sebelum sapih (0.04), PBB setelah sapih (0.06) and bobot badan pada umur 42 hari (2.30). Kesimpulan penelitian ini adalah nilai paling baik untuk GCA ditunjukkan oleh Duroc, sementara itu SCA oleh persilangan antara Yorkshire x Landrace.

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