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INDONESIA
JURNAL SAINS DAN TEKNOLOGI INDONESIA
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Articles 12 Documents
Search results for , issue "Vol. 14 No. 3 (2012)" : 12 Documents clear
PERTUMBUHAN, KUANTITAS DAN KUALITAS RIMPANG JAHE (Zingiber officinale Roscoe) PADA CEKAMAN KEKERINGAN DI BAWAH NAUNGAN Devy, Lukita; Nawfetrias, Winda
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.444 KB) | DOI: 10.29122/jsti.v14i3.929

Abstract

This research studied the growth, quantity and quality of ginger rhizome under drought stress condition. The drought stress condition was 60% of soil field capacity. This experiment was arranged in randomized complete block designed. The treatments were drought stress periods (6, 4, 2 and 0 week before harvesting) with six replications. The result showed that drought stress period affected the quantity (rhizome dry weight) and quality (gingerol content) of ginger. Rhizome dry weight was decreased with the increase of drought stress period, while gingerol content showed opposite trend.
SINTESIS DAN KARAKTERISASI NANOPARTIKEL KITOSAN – EKSTRAK KULIT BUAH MANGGIS (Garcinia Mangostana) rismana, eriawan; Kusumaningrum, susi; p, olivia bunga; rosidah, idah; marhamah, marhamah
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.139 KB) | DOI: 10.29122/jsti.v14i3.925

Abstract

The chitosan – Garcinia Mangostana extract nanoparticles has been prepared by ionic gelation reaction by mixture 0.2 % chitosan solution in acetic acid with Garcinia Mangostana extract and it’s continued by reaction process with 0.1 % sodium tripolyphosphate. The particle size of material was determined by Particle Size Analyzer (PSA) that it showed in the range of 200 – 500 nm. The color, pH, water, α- mangostin, mercury, arsenic, cadmium, lead, totally microbe aerobic, totally mold and yeast, and solvent residue contents of nanoparticles were also examined by many methods that these resulted are yellow, 4.50 – 5.50, 89 – 90 %, 1.05 %, < 0.005 ppm, < 0.01 ppm, < 0.01 ppm, < 0.05 ppm, < 10 CFU/g, < 10 CFU/g and not detected, respectively. The other characterization was also observed that it’sincluded stability andTLC chromatogram. A mixture of nanoparticles with cosmetics bases was showed that it’s increased stability, homogeneity and easy to formed.
AKTIVITAS FITOHORMON INDOLE-3-ACETIC ACID (IAA) DARI BEBERAPA ISOLAT BAKTERI RIZOSFER DAN ENDOFIT sukmadi, R bambang
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.08 KB) | DOI: 10.29122/jsti.v14i3.930

Abstract

A total of 34 isolates of rhizosphere bacteria and endophytic bacteria have been isolated from several fruit and vegetable plants. Bacterial isolates were then screened to produce phytohormone indole-3-acetic acid (IAA) in a minimal medium (MM) with the addition of 1 g/l tryptophan as a precursor. Phytohormone production was carried out in an incubator shaker for 5 days at 28oC in a dark condition. IAA in the culture supernatant was extracted with ethyl acetate. The presence of IAA in the bacterial extract was detected using Thin Layer Chromatography (TLC). The concentration of IAA was determined quantitatively using High Performance LiquidChromatography (HPLC). The result showed that among 34 bacterial isolates, 20 isolates were able to produce IAA with different concentrations. The highest concentration of IAA (16,71 ppm) was produced by the endophytic bacteria isolated from twigs of starfruit plant. In addition, within the group of rhizosphere bacterial isolates, bacteria from kangkung plant was found to be the best one with IAA concentration of 10.99 ppm.
PENGARUH ZEOLIT DALAM PUPUK TERHADAP PERTUMBUHAN DAN PRODUKSI PADI SAWAH DI KABUPATEN BADUNG PROVINSI BALI jufri, akhmad; Rosjidi, Mochamad
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.888 KB) | DOI: 10.29122/jsti.v14i3.921

Abstract

Many efforts are conducted to increase the fertilising efficiency, especially nitrogen in rice cultivation practices. This field experiment was conducted to evaluate the effect of zeolite on growth and production of lowland rice during rainy season in Badung, Bali. The experiment showed that fertilizer mixed with zeolite resulted in the same growth and productivity, although the fertilising doses was reduced. This means that zeolite incread fertilising efficiency in rice cultivation.
PENGARUH MALTOSA SEBAGAI KRIOPROTEKTAN EKSTRASELULER DALAM MENINGKATKAN KUALITAS SEMEN BEKU GUNA MENDUKUNG KEBERHASILAN TEKNOLOGI INSEMINASI BUATAN herdis, herdis; darmawan, i wayan angga
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.82 KB) | DOI: 10.29122/jsti.v14i3.926

Abstract

The research carried out to observe the effect of maltose addition on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature garut rams. Semen was equilibrated at 5oC for four hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. The result showed that percentages of viable sperm for addition of maltose 1,2 g / 100 ml extender (68,50 ± 0,84%) was significantly difference (P<0,05) than control (54,83 ± 1,94%) and addition of maltose 0,6 g / 100 ml extender (65,67 ± 1,03 %). The percentages of progressive motile sperm and percentages of plasma membrane for addition of maltose 1,2 g / 100 ml extender (53% and 64,67%) were significantly difference (P<0,05) than control (43% and 53,83%) but were not significantly different (P>0,05) from addition of maltose 0,6 g / 100 ml extender (50,83% and 64,67%)respectively. In conclusion, the addition of maltose 1,2 g / 100 ml extender is optimal dose to improve the quality of frozen semen of garut rams.
ISOLASI, IDENTIFIKASI, DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI DADIH SUSU KERBAU sunaryanto, rofiq; marwoto, bambang
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.184 KB) | DOI: 10.29122/jsti.v14i3.931

Abstract

Dadih is one of the traditional fermented milk products of West Sumatera. Dadih contain a lot of lactic acid bacteria that acts as a coagulant and preservative. Some lactic acid bacteria also act as a probiotic agent because of characteristics that are resistant to acidic conditions. Some of the main requirements of microbes that can be used as probiotic microbes were resistant to low pH, bile salts, able to colonize, and having antimicrobial activity. Each species of the genus Lactobacillus havedifferent characteristics. This characteristic are influenced by the environment in which the bacteria live. Isolation, identification and characterization of lactic acid bacteria derived from buffalo milk were done. The results of isolated lactic acid bacteria was Lactobacillus plantarum. The characterization of Lactobacillus plantarum indicated that these isolates were able to survive in a concentration of medium containing 0.5% bile salts, resistant to acidic media until pH 2, have antimicrobial activity (inhibit Escherichia coli, Staphylococcus aureus and Enterococcus faecalis).
PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana) Mulyono, Daru
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.761 KB) | DOI: 10.29122/jsti.v14i3.922

Abstract

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.
PEMANFAATAN BAHAN TEKNIS KNO3, CaCl2, MgSO4, KH2PO4 SEBAGAI HARA MAKRO DAN Benzil Adenin DALAM PERBANYAKAN JATI (Tectona grandis L) SECARA IN VITRO karyanti, karyanti; royani, juwartina ida
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.535 KB) | DOI: 10.29122/jsti.v14i3.927

Abstract

Indonesia is a major producer of teak after India and will gradually decrease if not followed by replanting. In general, teak plants propagated through seeds but have many disadvantages. Teak plant propagation using in vitro technique being an alternative to get the mass production of teak clones. In vitro technique, to some extent, needs a high cost particularly in using pure chemical substance. As an alternative solution, pure chemical substance can be substituted by using a few technique chemical substances such as KNO3, CaCl2, MgSO4, and KH2PO4. The aim of this research was to evaluated induction of shoot teaks, planted on different media with macro element substituted by different chemicals such as KNO3, CaCl2, MgSO4, and KH2PO4. The result showed that using the same concentration between 2 different chemical substances on teak shoot induction, there was no different shoot growing in teak propagated between 2 media.
BIOKOMPOS CAIR DAN PUPUK KIMIA NPK SEBAGAI ALTERNATIF NUTRISI PADA BUDIDAYA TANAMAN CAISIM TEKNIK HIDROPONIK Djamhari, Sudaryanto
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.003 KB) | DOI: 10.29122/jsti.v14i3.932

Abstract

Technique hydroponic system with Nutrient Film Technique (NFT), is one way of vegetable crops that can be done in a narrow and without the use of soil media. Nutrition is done by pouring so that it can carry oxygen (O2) from the air. Generally fertilizer nutrient used in hydroponic technique is AB-mix, this study sought to examine alternative combinations of NPK fertilizer and liquid biokompos on some degree of concentration thatcan provide optimum production growth in crop varieties caisim Tosakan. This experiment used Completely Randomized Design (CRD) with 4 treatments. Data analysis was performed using different test each plant and resumed Duncan Multiple Test Distance otherwise known Duncan's Multiple Range Test (DMRT) at 5% level (F count> F table orP> 0.05) was conducted to determine the extent of the effect is real or not experiment treatmentResearch results indicate that administration of a mixture of NPK fertilizer and liquid fertilizer biokompos An1O1 the treatment can be used as a substitute for AB-mix in the provision of nutrition to the cultivation of varieties caisim Tosakan with hydroponictechniques. An1O1 treatment is the best dose when compared to the control treatment AB-mix, An2O2, and An3O3 the highest production yield component (gross production amounted to 97.67 g / plant and 85.75 g CP / tan).
Analisis Keragaman Genetik Temulawak (Curcuma xanthorrhiza Roxb.) Menggunakan Penanda Amplified Fragment Length Polymorphism (AFLP) damayanti, dini; tajuddin, teuku; purwoko, devit; zulaeha, siti; suharsono, suharsono
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.369 KB) | DOI: 10.29122/jsti.v14i3.923

Abstract

Curcuma xanthorrhiza Roxb, which is well-known as Java turmeric, has been extensively used in pharmaceutical industries in Indonesia. In spite of this commercial value, the identity of this species is commonly mistaken from other similar orange rhizomes Curcuma. Correct identity of these species is vital in pharmaceutical industries. The objective of the study was to determine genetic diversity of 32 accession Curcuma xanthorrhiza Roxb. Genomic DNA was extracted from leaf using Sodium Dodesyl Sulphate (SDS) modification. Amplified fragment length polymorphism (AFLP) was carried out according to the protocol ofAFLPTM plant mapping kit and the final polymerase chain reaction (PCR) products were separated using The Agilent 2100 Bioanalyzer. The number of fragment produced by 12 pairs primer combination of AFLP ranged from 42 to 60 with an average of 52. Data obtained was analyzed by the NTSys program. From the AFLP amplification on 32 DNA samples, it was proven that the accession of Curcuma xanthorrhiza Roxb. had a high degree of diversity. Based on analysis of AFLP and unweighted pair group with arithme average (UPGMA) it was shown that the accession of Curcuma xanthorrhiza Roxb. could be grouped into two cluster at relative ecludian distance of 0.10 (10%). Cluster I for accession from Palembang, Pacitan and Ciamis 2. Cluster II for accession from Makale, Pontianak, Kulonprogo, Mataram, Boyolali, Salatiga, Sumberejo, Bali, P. Seram, Sentolo, Purworejo, Samas Bantul, Ciamis1, Blora, Semarang, Poso, Kalsesl, Tagari, Merapi Farm, Salakaria, NTB, Menoreh, Karang Anyar, Mangunan, Medan, Toraja, dan Solok.

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