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Norma Tiku Kambuno
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Laboratory Journal of Infectious Diseases
Published by Poltekkes Kemenkes Kupang
ISSN : 31239005     EISSN : 31239005     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Aim and Scope The primary goal of the LJID journal is to advance the understanding and knowledge of infectious diseases by publishing high-quality research focused on laboratory-based investigations. We aim to serve as a platform for discovering and exploring biomarkers include markers of Hematology, Clinical Chemistry, Immunoserology, Microbiology, Parasitology, Cyto-Histotechnology, and Molecular Biology for infectious diseases. Our scope includes significant findings in public health and epidemiology that are supported by laboratory data. This includes both emerging and established diseases, with a particular focus on their prevalence and characteristics in Indonesia and globally. By disseminating these findings, we hope to contribute to better prevention, diagnosis, and treatment strategies worldwide. Manuscript of original research should be written in 4,000-8,000 words (including tables and figures).
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 12 Documents
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Pediculosis capitis in Elementary School Children in Kupang Regency Michael Bhadi Bia; Aldiana Astuti; Lidia Vartika Putri; Noldiana Rini Hello; Junita Mogu Wole
Laboratory Journal of Infectious Diseases Vol. 1 No. 1 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/ljid.v1i1.2157

Abstract

Pediculosis capitis is a parasitic infestation caused by the head louse, Pediculus humanus capitis (PHC). These lice feed on human blood on the head. Head lice infestations most commonly occur among preschool and primary school children, as well as household members and caregivers. This study was conducted to provide an overview of the evidence regarding the prevalence of Pediculosis capitis among primary school students in Kupang Regency, East Nusa Tenggara. This study aimed to determine the prevalence of Pediculosis capitis and identify risk factors influencing its occurrence among primary school students in Kupang Regency. The study was conducted in primary schools in Kupang Regency during the 2023-2024 academic year. To identify cases of pediculosis, the presence of eggs/nymphs/adult Pediculus humanus capitis was detected using a lice comb. Clinical symptoms and signs were collected through interviews using a questionnaire and direct observation of clinical signs. A total of 278 children participated in the study, consisting of 164 boys (59%) and 114 girls (41%). Pediculosis cases were found in 151 children (54,3%). Characteristics of children influencing the occurrence of pediculosis include hair length and the number of family members living in the same household. Reported symptoms associated with pediculosis cases included dandruff, scalp itching, redness, difficulty sleeping, and poor concentration. Behaviours of primary school children related to the transmission of pediculosis include sleeping with an infected person, sharing hats, sharing combs at home, frequency of using a lice comb, and frequency of washing hair per week.
Inhibitory Effect of Moringa Leaf Extract on Escherichia coli Isolated from Urine of UTI Patients Daniela Tri Soge; Yoan Novicadlitha; Wilhelmus Olin; Norma Tiku Kambuno
Laboratory Journal of Infectious Diseases Vol. 1 No. 1 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/ljid.v1i1.2160

Abstract

The moringa plant (Moringa oleifera) are a well-known medicinal plant in Indonesia, often referred to as the “Miracle Tree” due to their numerous health benefits. Urinary Tract Infection (UTI) is a clinical condition in which bacteria are present in the urinary tract, typically caused by microorganisms such as Escherichia coli (E. coli). Moringa leaves contain antibacterial phytochemicals such as flavonoids, saponins, alkaloids, terpenoids, steroids, catechol tannins, gallic tannins, and anthraquinones, which can inhibit the growth of E. coli. UTIs are commonly treated with antibiotics, but excessive use of antibiotic can have consequence, such as antibiotic resistance. This study aims to determine the inhibitory activity of moringa leaf extract against E. coli isolated from the urine of UTI patients. The bacteria were cultured using EMBA media, Gram staining, and biochemical tests. The extract was prepared through maceration, and the Minimum Inhibitory Concentration (MIC) was determined using the broth dilution method. The MIC test used bacterial suspensions at McFarland 0,5 and 0,25 with extract concentrations of 0,25%, 0,5%, 1%, 5%, 10%, 20%, 30%, and 40%. Results were observed using the turbidimetry method or visual observation. The findings showed that moringa leaf extract inhibited E. coli growth at concentrations of 20%, 30%, and 40%, as the suspensions remained clear after incubation. Conclusion: Moringa leaf extract can inhibit E. coli growth isolated from UTI patient urine and may be recommended for UTI prevention and treatment. These results are expected to provide knowledge that moringa leaves can be processed as food ingredients that have the potential to be used in efforts to prevent UTIs.
Examination of Total Cholesterol Levels in the Elderly with Hypertension (Case Study at Abiansemal I Health Center, Badung Regency-Bali) Komang Ayu Adinda Paramitha Triyani; Heri Setiyo Bekti; I Gusti Agung Ayu Dharmawati
Laboratory Journal of Infectious Diseases Vol. 1 No. 1 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/ljid.v1i1.2246

Abstract

An elderly person is someone who is 60 years or older. As we age, changes in the structure and function of cells, tissues, and organ systems occur in the body, increasing the risk of hypertension. Hypertension is a multifactorial disease that can be caused by cholesterol. Hypercholesterolemia can clog peripheral blood vessels, thereby triggering hypertension. The purpose of this study was to describe total cholesterols levels among elderly individuals with hypertension at Abiansemal I Health Center, Badung Regency, Bali. This type of study used is descriptive research using non-probability techniques, purposive sampling. Total cholesterol levels were measured using the POCT method in 44 elderly participants with hypertension; 14 individuals had borderline cholesterol levels, and 17 individuals (38,6%) had high total cholesterol levels. Based on the characteristics of the respondent's dominant gender with High cholesterol levels in women were 10 people (22,7%). Based on Body mass index (BMI) is dominant among respondents with high total cholesterol levels in the normal BMI category there were 11 people (25,0%). It can be concluded that most elderly individuals with hypertension at Abiansemal I Community Health Center had high total cholesterol levels.
Extract of Teak Leaf Buds (Tectona grandis) as Natural Dyes Replacement of Eosin on HE Staining Adrianus Ola wuan; Angelyca Florestra Tulit Bali; Ni Ketut Yuliana Sari
Laboratory Journal of Infectious Diseases Vol. 1 No. 1 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/ljid.v1i1.2247

Abstract

Hematoxylin and Eosin (HE) staining is a routine method in histopathology. In HE staining, eosin functions to stain the cytoplasm of the tissue. Continuous use of eosin may cause health problems due to its carcinogenic properties, thus requiring an alternative. One such alternative is teak leaf buds, which contain anthocyanin compounds. The aim of this study was to determine whether teak leaf bud extract can be used as a substitute for eosin in HE staining. The type of research used was a true experimental study with a post-test only control group design. The samples consisted of paraffin blocks, from which 10 slides were prepared: one control and nine treatment slides, with three repetitions for each concentration (20%, 30%, and 40%). Microscopic observations of HE staining using eosin showed a score of 3 (100%). Using 20% teak bud extract resulted in a score of 2 (100%), 30% concentration gave a score of 2 (33,3%) and score 3 (66,7%), while 40% concentration resulted in score 3 (100%). Kruskal-Wallis test showed eosin and teak leaf bud extract were not significantly different: for 20% concentration, p=0,083; for 30%, p=0,564; and for 40%, p=1,000 (p>0,05), indicating no significant difference.It can be concluded that 40% teak leaf bud extract is the best concentration and has the potential to replace eosin in HE staining. Future research is suggested to use 40% teak leaf bud extract with variations in staining time to further explore its potential as a substitute for eosin in histological staining.
Profile of Atherogenic Index in Elderly Individuals in Bitoa Village, Manggala District Vera Kristianti Woso; Mutmainnah Abbas; Thaslifa Thaslifa; Rosdiana Mus
Laboratory Journal of Infectious Diseases Vol. 1 No. 1 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/ljid.v1i1.2253

Abstract

The atherogenic index is a good test used to measure the level of atherogenicity associated with the risk of atherosclerosis. The atherogenic index is the logarithmic ratio of TG/HDL. Atherogenic index examination in the elderly is done to see their health status. Elderly is someone who has reached the age of more than 60 years and has experienced several changes in physiological functions that will have an impact physical and psychological conditions, including stress and emotions. The purpose of this study was to describe the profile of the atherogenic index in elderly individuals. This type of research is descriptive study with a cross-sectional approach. The amount of data used in this study were 36 subjects obtained using the purposive sampling method. The results of the study at the age of 60-64 who had the resuts of the atherogenic index in the low category there were 6 respondents and the results of the atherogenic index in the moderate category there were 8 respondents, while for atherogenic index in the high category there were 16 respondents. Ages 65-69 obtained an atherogenic index in the low category, with one respondent in the high category, comprising 5 respondents. The conclusion of this study is that the atherogenic index value at the age of 60-69 obtained an atherogenic index in the low category; there were as many as 21 respondents, and the results of the atherogenic index in the moderate category were 8 respondents, while for the atherogenic index in the high category, there were 21 respondents.
Differences in Pre- and Post-Menstruation Hemoglobin Levels in Students of the Medical Laboratory Technology Study Program at Poltekkes Kemenkes Kupang Flistiana Lobain; Novian Agni Yudhaswara; Aldiana Astuti
Laboratory Journal of Infectious Diseases Vol. 1 No. 1 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/ljid.v1i1.2254

Abstract

Anemia is a common health problem experienced by adolescent girls, especially due to blood loss during menstruation which causes a decrease in hemoglobin (Hb) levels. Hemoglobin is an important protein in red blood cells that functions to transport oxygen from the lungs to all body tissues. Lack of hemoglobin can disrupt body function and potentially cause the risk of ongoing mental complications. This study aims to identify differences in hemoglobin (Hb) levels before (pre) and after (post) menstruation among female students of the Medical Laboratory Technology Study Program at Poltekkes Kemenkes Kupang. The research method used is quantitative with a cross-sectional approach. The sample in this study consisted of 30 female students who were taken using random sampling techniques. Hemoglobin levels were measured twice, namely on the first day of menstruation and after the last day of menstruation, using a POCT (Point of Care Testing) tool that provides fast and practical results. Data analysis was carried out using the Wilcoxon test because the data obtained were not normally distributed. The results of the study showed a significant decrease in hemoglobin levels after menstruation. Before menstruation, the average Hb level was recorded at 12,847 g/dL, while after menstruation it decreased to 11,273 g/dL. The Wilcoxon test results showed a significance value of 0,000 (p<0,05), which indicated a significant difference between hemoglobin levels before and after menstruation.
Correlation of Anemia Status in Mothers and Stunted Toddlers Florida Ester Pailau; Michael Bhadi Bia; Supriati Wila Djami; Ni Ketut Yuliana Sari; Atun Farihatun
Laboratory Journal of Infectious Diseases Vol. 1 No. 2 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

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Abstract

Postpartum anemia remains a public health concern due to its potential impact on maternal and child health. This study aimed to analyze the correlation between maternal anemia status and anemia among stunted toddlers. An analytical observational study with a cross-sectional design was conducted at Pustu Noelbaki. The study subjects were mothers and their stunted toddlers selected using purposive sampling. A total of 40 participants were included, consisting of 20 mothers and 20 stunted toddlers. Maternal and toddler anemia status were assessed and analyzed using statistical correlation testing with a significance level of p < 0,05. The results showed that 25% of mothers were anemic, while 55% of stunted toddlers had anemia. Statistical analysis demonstrated a p-value of 0.319 (p > 0,05), indicating no significant correlation between maternal anemia status and anemia among stunted toddlers. In conclusion, maternal anemia was not significantly associated with anemia in stunted toddlers in this study. Further research with larger sample sizes is recommended to better understand potential contributing factors.
Analysis of Lead (Plumbum) Metal Levels in the Urine of Motorcycle Mechanics in Medan Helvetia District, Medan City Ziza Fahriza; Dyna Grace Romatua Aruan
Laboratory Journal of Infectious Diseases Vol. 1 No. 2 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

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Abstract

Lead (Pb) is one of the pollutants originating from vehicle exhaust emissions and materials from vehicle components, such as gasoline, oil, and used batteries. Workshop workers are among the occupations that are highly vulnerable to lead exposure, as their daily activities involve continuous exposure to vehicle exhaust fumes and direct contact with materials from vehicle components during work, thereby increasing the risk of lead exposure. Sampling was conducted at motorcycle workshops located on Jalan Gaperta in Medan City, North Sumatra, and the measurement of lead levels in urine was carried out at the Regional Health Laboratory in Medan City. The purpose of this study was to analyze lead (Pb) levels in the urine of motorcycle workshop workers on Jalan Gaperta, Medan, North Sumatra. The method used in this study was Atomic Absorption Spectrophotometry (AAS). The samples consisted of urine from ten motorcycle workshop workers. The results showed that the highest lead level was found in sample Z04, at 52,5 µg/dL, while the lowest lead level was found in sample Z02, at 3,5 µg/dL. These results indicate that the lead levels in some motorcycle workshop workers exceeded the threshold limit established in the Regulation of the Minister of Health of the Republic of Indonesia No. 1406/MENKES/SK/IX/2002, which states that the permissible threshold limit for lead levels in urine specimens is 10-25 µg/dL.
Knowledge of Mothers and Anemia Status of Stunting Children at Oepoi Community Health Center Valeria Saubaki; Wilhelmus Olin; Yoan Novicadlitha; Retno Martini Widhyasih
Laboratory Journal of Infectious Diseases Vol. 1 No. 2 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

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Abstract

Anemia is a significant factor contributing to stunting, affecting maternal capabilities in childcare, including energy levels and breast milk quality. This descriptive quantitative study was conducted at the Nyiur and Seroja integrated service posts within the Oepoi Community Health Center in Kupang City. Total sampling was employed, involving 44 respondents comprising mothers and children diagnosed with stunting. Among child respondents, 12 (55%) were male and 10 (45%) were female. The most represented age group was > 2-3 years (15 samples, or 68,18%), while the > 3-5 years group had 7 samples (31,81%). Hemoglobin levels indicated that 8 children (36%) were in the abnormal range, while 14 (64%) were normal. All maternal respondents (22 samples, 100%) were aged 20 years and above. Maternal knowledge assessment revealed that 13 respondents (59,09%) had good knowledge, while 9 (40,91%) had poor knowledge regarding anemia. These findings highlight the notable prevalence of anemia among mothers and children in the study area, emphasizing the need for targeted interventions to address this issue, as it significantly impacts child health and development. Addressing anemia in mothers and children is crucial to combat stunting and improve overall health outcomes in the community.
Identification of Bacteria in Foot Wound Swabs from Patients Diabetes Mellitus Nurul Humairoh; Ni Nyoman Ariwidiani; Baiq Isti Hijriani
Laboratory Journal of Infectious Diseases Vol. 1 No. 2 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

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Abstract

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia that predisposes patients to impaired wound healing and chronic infections, including diabetic foot ulcers. Bacterial colonization of these wounds plays a critical role in delayed healing, increased morbidity, and risk of amputation. This study aimed to identify the bacterial profiles isolated from foot wound swabs of patients with diabetes mellitus. A descriptive observational study was conducted using foot wound swab samples collected from patients with diabetes mellitus receiving wound care at Mandalika Care, Mataram City. Bacterial identification was performed through macroscopic and microscopic examination, Gram staining, and standard biochemical tests following culture on Blood Agar Plate (BAP) and MacConkey Agar (MCA). Data were analyzed descriptively and presented as frequencies and percentages. Six samples met the inclusion criteria and were analyzed. All isolates were Gram-negative bacteria. The bacterial species identified were Pseudomonas aeruginosa (33,36%), Morganella morganii (16,6%), Pseudomonas putrefaciens (16,6%), Enterobacter agglomerans (16,6%), and Pseudomonas mallei (16,6%). The conclusion is that Gram-negative bacteria predominated in diabetic foot wound infections in this study, with Pseudomonas aeruginosa being the most frequently isolated species. These findings highlight the importance of early microbiological examination to guide appropriate wound management and antimicrobial therapy in diabetic patients.

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