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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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Genotypic Characterization of Rhizopus Spp. Tempeh and Usar: Traditional Inoculum of Tempeh in Indonesia tati barus; Jason Wiranata Sanjaya; Anastasia Tatik Hartanti; Adi Yulandi; Vivitri Dewi Prasasty; David Tandjung
Microbiology Indonesia Vol. 14 No. 3 (2020): September 2020
Publisher : Indonesian Society for microbiology

Abstract. Soybeans tempeh (tempeh) is processed by fermentation using Rhizopus spp. Tempeh is an important source of protein in Indonesia. The traditional inoculum in fermentation locally is known as Usar which is made from the leaves of Hibiscus tiliaceus. However, Rhizopus information from Usar is still limited. Therefore, this study aims to identify and investigate the genetic diversity of Rhizopus species from Usar and tempeh based on the Internal Transcribed Spacer (ITS) sequence and the Random Amplified Polymorphic DNA (RAPD) markers. Twenty-three Rhizopus strains were isolated from Usar and ten Rhizopus strains were isolated from tempeh. Based on ITS sequences, the isolates were similar to R Rhizopus microsporus (30 isolates) and Rhizopus delemar (3 isolates) with 98-99% similarity. The genetics of R. microsporus and R. delemar are varied and different from the genetics of R. microsporus from tempeh. The growth temperature of R. microsporus varies from 33 to 48Â°C and R. delemar can grow to a maximum at 33Â°C. The role of R. microsporus and R. delemar from Usar in determining the quality of tempeh is still limited. Therefore, it needs to be investigated further.

The Antifungal Activity of Artesunate toward Candida albicans: Two Opposite Activities MOHAMMAD RIZKI F ADHIL PRATAMA; DARYONO HADI TJAHJONO; TUTUS GUSDINAR
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

The artemisinin and its derivatives antifungal activity continue to be an interesting research object, with the potential shown to be developed as an antifungal compound. Artesunate, one of the artemisinin derivatives known to have antifungal activity against various pathogenic fungi, including Candida albicans. This study aims to determine the effect of artesunate on antifungal activity toward C. albicans in vitro at concentrations below 1 mg -1m L . The method used is yeast-plate count, with a parameter of observation were the number of C. albicans colonies viable after exposure with artesunate for five days. The concentration of artesunate used was divided into -1 -2 -3 -4 -1si x groups, which were 10; 1; 10 ; 10 ; 10 ; and 10 mg mL . Compared to control, a significant decrease in -1c olony counts was only shown at the highest concentration of 10 mg mL . Interestingly, at the lowest -4 -1c oncentration of 10 mgmL , it showed an increase in a number of colonies almost twice of the blank. These results suggest that while at higher concentration of artesunate may inhibit the growth of C. albicans, a lower concentration of artesunate may stimulate their growth.

Prevalence of Hepatitis B Virus Infection in Blood Donors Based on Titer Hepatitis B Surface Antigen Examination (HBsAg) SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Hepatitis B remains a global public health problem. Infection from hepatitis B virus (HBV) can be transmitted through a blood test or a blood transfusion. This study was conducted to identify the prevalence of HBV infection in blood donors based on examination of HBsAg titers . Blood donors from Tuban Red Cross used as sample. The method used in this research is HBsAg titers examination performed by ELISA according to the procedureoutlined in the Kit. HBsAg titers positive mostly found in men. In men from 13 samples (8.67%) are HBsAg titers positive of 150 samples while in woman all negative for HBsAg titers from 137 samples. The average titer positive was 3.095 with a standard deviation of 0.187. While HBsAg titers negative have average of 0.03 with a standard deviation of 0.14. This study showed that the prevalence of HBV infection in blood donors is most numerous in men with HBsAg titers positive number of 8.67%.

Gene Cloning of Xylanase Glycoside Hydrolase Family 11 from Bacillus halodurans CM1 in Escherichia coli DH5Î± Muhamad Taufiqul Naufal; Agustin Krisna Wardani; IS HELIANTI
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Xylanase is an enzyme that can break down xylan into xylose and xylooligosaccharide that is widely used in industry. Seeing the many applications of this enzyme, researchers conducted many studies on how to increase the productivity and effectiveness of the xylanase enzyme. One of the method that can be used to increase the xylanase enzyme production process is by using recombinant DNA technology such as cloning. Bacillus halodurans CM1 is a local alkalothermophilic bacterium that potential producer for xylanase and other industrial enzymes. This research was conducted to clone the GH11 xylanase coding gene from B. halodurans CM1 using pJET 1.2 / blunt plasmid as vector into Escherichia coli DH5Î± as cell host andÂ determine the nucleotide base sequence of the GH11 xylanase coding gene from B. halodurans CM1. The results showed the GH11 xylanase gene from B. halodurans CM1 was successfully cloned inÂ E. coli DH5Î± and based on the results of BLAST nucleotides had 99% similarities with that of endo-1,4-beta -xylanhydrolase (xyn11A) from B. halodurans C-125. Key words: Bacillus halodurans CM1, cloning, xylanase glycoside hydrolase family 11

Influence of indigenous mixotrophic bacteria on pyrite surface chemistry: Implications for bioflotation Edy Sanwani; Nuslia Bayangkara Lamandhi; Halimatul Husni; Siti Khodijah Chaerun; Widi Astuti; Fika Rofiek Mufakhir
Microbiology Indonesia Vol. 14 No. 1 (2020): March 2020
Publisher : Indonesian Society for microbiology

Given the low-cost and eco-friendly method, biotechnology has been widely utilized in industries as an alternative for physical and chemical processes, including in the biomining process (e.g., bioflotation and biobeneficiation). However, the use of biochemical reagent, which is selective for certain minerals, has not been well studied. This research was aimed to investigate the potential use of biosurfactant-producing mixotrophic bacteria as an alternative to chemical reagents during bioflotation and biobeneficiation process. Thirteen bacterial strains were investigated for their ability to produce biosurfactants and their effects on the surface properties of pyrite minerals. Bacteria-pyrite interaction experimental results showed that pyrite surface properties became more hydrophilic in the experimental systems inoculated with bacteria adapted with pyrite for 48 h than that without bacterial adaptation to pyrite, which was evidenced by the decrease in the contact angle of pyrite minerals by up to 50%. This evidence was also confirmed by the highest emulsifying index value (51.6%) attained during the bacteria-pyrite interaction. Hence, these bacteria can potentially be applied to selective flotation as pyrite depressants.

The utilization of auto-inducible Plyb promoter and media optimation for cell density-dependent expression of recombinant xylanase in Bacillus subtilis DB104 Haniyya Haniyya; Dini Achnafani; Maria Ulfah; Niknik Nurhayati; Is Helianti
Microbiology Indonesia Vol. 14 No. 1 (2020): March 2020
Publisher : Indonesian Society for microbiology

Strong promoters are one of the fundamental aspects to increase the level of gene expression, and one of approach to improve the recombinant enzyme productivity so that the efficiency of production cost for enzyme production in industrial scale can be reached. Here we assessed the application of a cell density-dependent promoter and media optimation to promote cell growth and protein expression of Bacillus subtilis without excess usage of inducers. An auto-inducible Pylb promoter that is potential to provide inducer-free enzyme production was cloned and introduced into xylanase recombinant system in B. subtilis DB104 by PCR cloning and protoplast transformation. A 200 bp target gene was successfully inserted in between xynCM1 ORF -coding for B. halodurans CM1 xylanase- and its native promoter sequence at the upstream region. The disruption of the native promoter was intended to replace the native promoter with Pylb. Recombinant xylanase gene under Pylb was successfully expressed in B. subtilis DB104 and the enzyme was produced at stationary phase. Different media with various concentrations of glucose and nitrogen were used to optimize recombinant xylanase expression. It achieved a higher level of xylanase expression compared to wild-type and recombinant xylanase with native promoter B. subtilis in media containing a 2-fold recipe of LB media thus leads to increase cell density and xylanase expression (81.461 U mL-1).

Utilization of Leaves in Mine Reclamation Land as Organic Fertilizer with Effective Bioactivatory of Microorganism 4 (em4) and Molasses Supiana Dian Nurtjahyani; Dwi Oktafitria; Sriwulan Sriwulan; Nova Maulidina; Imas Cintamulya; Eko Purnomo
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Organic fertilizer as an alternative to reduce the scarcity of chemical fertilizers, otherwise it can improve soil fertility. PT. Semen Gresik - Semen Indonesia Persero (Tbk.) Has a lot of plants in the treatment period in ex-mining land that needs fertilizer to help plant growth. In addition, in post-mining land, there is a lot of organic waste in the form of dry leaves under the stands of reclamation trees. The organic waste has the potential to be processed and used as organic fertilizer. This study aims to determine the utilize leaf litter in the reclaimed mine land as organic fertilizer with EM 4 and Molasses bioactivators. This study used is a laboratory experimental method. The results showed the composition of solid material in the form of leaf litter, bran, cow dung and husk charcoal in a ratio of 2: 1: 1: 1 as well as looking material in the form of molasses and EM4 with a dose of 100ml per 100kg of solid material dissolved in enough water (fertilizer can be clenched and expanded slowly and not broken). Conclusion leaf litter in the former reclaimed land can be processed and used as organic fertilizer.

Antibacterial Potential of Star Anise (Illicium verum Hook. f.) Against Food Pathogen Bacteria EVELINE EVELINE; AGUSTIN NOVITA
Microbiology Indonesia Vol. 14 No. 1 (2020): March 2020
Publisher : Indonesian Society for microbiology

Star anise (Illicium verum Hook. f.) is commonly used as spice and flavor enhancer in food. Previous research revealed the presence of active compound which could inhibit bacterial growth. Thus, in order to apply star anise as natural antibacterial agent in food product, a further research concerning antibacterial activity and stability of star anise was conducted. Crude extract of star anise was obtained using ethanol and acetone with maceration method for 3 days, then diluted to 10, 20, 30, 40, and 50% (w/v). Well diffusion was conducted against three food spoilage bacteria (Staphylococcus aureus, Escherichia coli, and Bacillus cereus). Extract from ethanol with 30% concentration was selected as the best extract in which inhibit more than 6 mm inhibition zone with MIC and MBC value: 1.59% and 6.36% (S. aureus), 1.04% and 4.18% (E. coli), and 0.59% and 2.39% (B. cereus). This selected extract was used to test the extract stability against 4 levels of heating temperature (60, 70, 80, and 90Â°C) for 2 levels of heating time (15 and 30 minutes), and 4 levels of pH (4, 5, 6, and 7). Based on our results, different heating treatment and pH caused extract instability. Star anise extract was more stable at 60Â°C for 15 minutes heating treatment and pH 4, which resulting the lowest inhibition zone reduction compared to control extract. Star anise extract was categorized as low toxic compound (LC50 = 212.09 ppm). Terpenoids (anethole, 2,6-dimethyl-6-(4-methyl-3-pentenyl)-2-norpinene, Î²-caryophyllene, Î²-bisabolene) was founded as major antibacterial compound in star anise extract; fatty acid (6-octadecenoic acid, hexadecanoic acid, stearic acid) and benzaldehyde (4-anisaldehyde, p-allylanisole) were also founded as minor compound.

The Effect Of Aeration Rate On The Growth Of Blue Green Microalgae in Buffalo Dung As Alternative Media EDWIN YONATHAN GURNING GURNING; AMOS IMANUEL; NINA JULIANA ROBERTA TURNIP; ADELINA MANURUNG
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

The high demand of Arthrospiraplatensis as a veritable protein source encourages its mass production worldwide. Currently, mass production of Arthrospiraplatensis is hindered by the relatively high price of the growth media. Recently, it is discovered that Arthrospiraplatensis can be cultivated using buffalo dung as an alternative medium. Buffalo dung is an excellent source of nitrogen and phosphorus which are principal macronutrients for the growth of Arthospiraplatensis. In addition to nitrogen and phosphorus, carbon is also a macronutrient that is important to the growth of microalgae. The carbon source used by the microalgae is carbon dioxide, which is consumed through photosynthesis. Carbon dioxide can be derived directly from the atmosphere as atmospheric CO2 existing as much as 0.04%-v/v in air, which can be provided directly using an aeration pump into the growth medium microalgae. During the aeration process, CO2 mass transfer occurs from the gaseous phase into the liquid phase. This research aims to investigate the effect of the aeration rate on the growth of the blue-green microalgae Arthrospiraplatensisusing buffalo dung media as an alternative medium. Arthrospiraplatensis will be cultivated on buffalo dung media using various aeration rates to determine the effect of aeration on the specific growth rate (Âµ). The air will also be pumped into the growth medium without Arthrospiraplatensis at the specific aeration rates to determine the mass transfer coefficient (kLa) that occurs from the air leading to growth medium. Analysis of mass transfer coefficient (kLa) of carbon dioxide will be conducted using the sulfite method. Variation of aeration that used in this research are 0.2 vvm; 0.4 vvm; 0.6 vvm; 1.2 vvm; 2.4 vvm that has mass transfer coefficient dan specific growth rate are 0.005 min-1 and 0.1987 day-1; 0.009 min-1 and 0.2279 day-1; 0.012 min-1 and 0.2044 day-1; 0.034 min-1 and 0.1918 day-1; 0.035 min-1 and Âµ in 2.4 vvm canâ€™t determine, respectively.

Antagonistic Effect of Two Indigenous Phosphate Solubilizing Bacteria, Burkholderia contaminans PSB3 and Acinetobacter baumannii PSB11 Isolated From Different Crop Soils RULLY ADI NUGROHO; VINCENTIA IRENE MEITINIARTI; CHRISSEPTINA DAMAYANTI
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Phosphorus is the most important key element in the nutrition of plants. Although P is abundant in soils, it is a major limiting factor for plant growth as it is in an unavailable form for roots uptake. Phosphate solubilizing bacteria (PSB) has ability to convert insoluble form of P to an available form. This study was aimed at screening and characterizing phosphate-solubilizing bacteria from manure and different rhizosphere and to ascertain a potential benefit to use mixed cultures to improve P solubilization. A total of 12 PSB colonies were isolated on Pikovskayaâ€™s agar medium containing tricalcium phosphate. Out of 12 bacterial isolates, 2 isolates showed high phosphate solubilization index (2.17 and 1.83, respectively) were selected for further study. Based on the 16S rRNA gene sequence analysis, PSB3 was closely related to Burkholderia contaminans (99%), and PSB11 was closely related to Acinetobacter baumannii (99%). The mean P dissolved in liquid cultures of PSB3 and PSB11 in a 14-day incubation were 96.7 and 39.3 mg l-1, respectively. Mixed inoculation of B. contaminans PSB3 and A. baumannii PSB11 could not increase the solubilization activity significantly, suggesting there is antagonistic behavior of one isolate towards another. As the interaction of these two isolates may be antagonistic, co-inoculation of these bacteria for P solubilization is not recommended. However, further study is needed to confirm these results.

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Iman Rusmana

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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

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Kota tangerang,

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INDONESIA

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Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA