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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Articles 520 Documents
Prevalence of mcr-1 Colistin Resistant Gene in Escherichia coli Along the Broiler Meat Supply Chain in Indonesia Palupi, Maria Fatima; Wibawan, I Wayan Teguh; Sudarnika, Etih; Maheshwari, Hera; Darusman, Huda Shalahudin
BIOTROPIA Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.2.1054

Abstract

Colistin is the last drug of choice for dealing with carbapenem-resistant Enterobacteriaceae bacteria; hence, this drug is very crucial to human health. The discovery of a plasmid-mediated colistin-resistant gene, the mobilized colistin resistance-1 (mcr-1), signals a significant global health threat. Colistin sulfate is an antimicrobial agent which has been approved for use in broilers in Indonesia. Thus, this study aimed to measure the prevalence of colistin-resistant Escherichia coli and to detect the mcr-1 colistin-resistant gene in E. coli and E. coli O157:H7 in the entire supply chain of broilers in Bogor Regency, West Java Province, Indonesia. Samples were taken from 47 flocks that used colistin sulfate (47 pools of cloacal swabs, 47 pools of drinking water, and 47 pools of litter), seventy fresh meat samples and seven plucker swab samples from seven small-scale poultry slaughterhouses, seventy fresh meat samples from seven traditional markets, and seventy cooked meat samples from seven small restaurants. The isolation of E. coli was done on each of the 358 samples, and 493 isolates were obtained. All the E. coli isolates were then tested for their susceptibility to colistin sulfate by using the agar dilution method. The detection of the mcr-1 gene from the colistin-resistant isolates (minimum inhibitory concentration > 2 µg/mL) was conducted using the polymerase chain reaction (PCR). The prevalence value of colistin-resistant E. coli in all the isolates was 11.76% (CI 95%; CL 9.21–14.91), and the prevalence of the mcr-1 gene was 10.55% (CI 95%; CL 8.13–13.57%). A very good agreement correlation existed between the colistin-resistant phenotype and the mcr-1 gene (κ = 0.939). The mcr-1 gene was found in 89.66% of colistin-resistant E. coli isolates. Two colistin-resistant and mcr-1 carrying isolates were identified as E. coli O157:H7 serotype. This research was the first study attempt on the mcr-1 gene in Indonesia, covering the entire supply chain of broiler meat from farms to consumers. The results indicated the necessity to reduce the use of colistin sulfate in broiler management and to improve biosecurity measures, not only in farms but also in the entire supply chain of broiler meat production.
SPECTRAL AND TEXTURAL CHARACTERISTICS OF THE LOWLAND TROPICAL RAIN FOREST OF JAMBI, SUMATERA WASRIN, UPIK ROSALINA; ARIEF, MUCHLISIN; SETIABUDHI, SETIABUDHI
BIOTROPIA No. 13 (1999)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.1999.0.13.152

Abstract

Analyses of Landsat TM and SPOT multispectral data were performed with a very detailed description of the vegetation cover in the field to get a relevancy and consistency of digital image classification in a semi-automatic approach. Three main vegetation types, i.e. primary forest, logged-over forest and secondary forest after clear cut were analyzed and the microclimatic parameters were also measured to describe the ecological condition of the vegetation. Spectral and textural analysis of data obtained from field measurements and spectral reflectance values of the remote sensing data are the main topic of this report as one aspect of study on the Digital Method of Detection and Monitoring on Forest Ecosystem Change Using High Resolution Satellite Data funded by the Indonesian National Research Council. This study shows that spectral reflectance values alone cannot differentiate the logged-over forest from the primary forest, but it is very sharply distinguished from the secondary forest. As for the texture analysis, it is possible to distinguish the logged-over forest from the primary forest, as shown by different values of degree of Entropy, although spatially, it is still doubtful. Key words: Indonesia/Jambi/Tropical rain forests/lowland areas/Remote sensing/Vegetation Analysis/ Logged-over forests/Primary forests/Secondary forests.
Two New Aphelenchoides Fischer 1894 (Nematoda: Tylenchina) from Manipur, North East India Chanu, Loukrakpam Bina; Mohilal, Naorem
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.377

Abstract

Two new species of Aphelenchoides discovered from different localities of Manipur, North East India had been described in the present study. Aphelenchoides longistylus sp. nov. has a long stylet and four lateral lines which are gradually merged into two at the tail region. Aphelenchoides neominoris sp. nov. has a small body and short tail with a stylet length of 8.6 µm.
LIPID PRODUCING MICROALGAE FROM SEVERAL ECOSYSTEMS IN WEST AND CENTRAL JAVA, INDONESIA SANTOSA, DWI ANDREAS; SULASTRI
BIOTROPIA Vol. 17 No. 2 (2010): BIOTROPIA Vol. 17 No. 2 December 2010
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2010.17.2.79

Abstract

This study is aimed to get lipid producing microalgae as feedstock for biofuel production. The microalgae were isolated from 355 collected water samples which  represented many distinct ecosystems such as paddy fields, rivers, agricultural dams, ponds, swampy areas and unique ecosystem of volcano and mud-volcano craters in West- and Central Java, Indonesia. A total of  267 strains of microalgae were isolated from the samples of which  221 strains of them have capability to produce lipid. There were four promising strains that produce lipid between 14.7 – 45.7 percent dry weight in optimal condition that were identified as Chlamydomonas sp. KO-7267 and PK-7195, Chlorella sp. KS-7300 and Desmodesmus sp. BK-7291.   Keywords: microalgae, lipid, biofuel, Indonesian ecosystems
EFFECTS OF THE HEAVY METAL, ZINC, ON THE FRESHWATER FISH TILAPIA NILOTICA L. S. CARING, VIRGINIA
BIOTROPIA No. 6 (1992)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.1992.0.6.108

Abstract

Gills, gonads, and blood of Tilapia nilotica exposed to different concentrations of zinc sulfate (ZnSO4. 7H2O) exhibited histological effects. Gills of posthatch larvae exposed chronically for 21 days to 2 ppm zinc sulfate and fingerlings to 10 ppm sublethal zinc concentrations exhibited hyperplasia that resulted in fusion of adjacent secondary gill lamellae. The same effects were observed in 4-hour short-term exposure to 30 ppm lethal dose. Posthatch larvae subjected to 2 and 5 ppm sublethal levels of zinc for 30 days retained undifferentiated gonads with differentiation with oogonial proliferation. Ovaries of control fish demonstrated healthy oocyte growth and other normal histological features after 57 days. In contrast, ovaries in treated groups exhibited excessive amounts of connective tissue, hyperemia and markedly reduced oocyte number. Oocytes had wavy irregular surface outlines. Deviation from normal was observed to be dose dependent. In juvenile tilapia, spermatogenesis was observed in control testes. Testes of zinc-exposed fish, on the other hand, remained immature. Hyperemia was markedly pronounced in both testes and ovary after 90 days exposure to zinc. Blood of Tilapia nilotica fingerlings exposed to sublethal concentrations of 2, 5, and 10 ppm zinc for 30, 60, and 90 days exhibited anisocytosis and poikilocytosis. There was an increase in hematocrit values in zinc-reared fish which, however, reverted to control/near control levels at day 90. Hemoglobin values were inversely proportional to the level of zinc in the rearing water. The marked reduction in hemoglobin values in fish reared at the higher zinc concentrations of 5 and 10 ppm suggests the development of some degree of anemia which is also supported by the observations of anisocytosis and poikilocytosis.
THE NATURAL HABITAT OF BEGONIA ON THE ISLAND OF BALI: MICROCLIMATE PREFERENCE IN TWO FOREST SITES IN BEDUGUL Satyanti, Annisa
BIOTROPIA Vol. 19 No. 2 (2012): BIOTROPIA Vol. 19 No. 2 December 2012
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2012.19.2.237

Abstract

A study on Begonia’s ecology was conducted in two forest sites, a nature reserve and reboisation forest, respectively, in Bedugul Bali. The objective of the study was to describe Begonia species found in respective forest sites, to gather information on abiotic factors and to find the influences of these abiotic factors to the abundance of Begonia species. Three species were identified as B. multangula, B. baliensis and B. longifolia. There were two form of B. longifolia, white and red. The multivariate analysis (PCA) showed that abiotic factors measured were relatively similar among plots. In addition, no particular abiotic factor, from multivariate analysis, showed specific influence on Begonia species frequency.  Interestingly, the multivariate analysis also implied that B. latifolia has different microclimate preference between forms (white and red).  The abundance, refers to the frequency, of all of species in two forests was similar (t-test, p=0.061). Pooling the data from the fourty plots (two forest sites) and white and red forms of B. longifolia together, B. multangula and B. longifolia found were similarly abundant between species (t- test, p=0.460).
Calamus vitiensis (Arecaceae), a New Record of Rattan in Sumbawa Island, Indonesia Rustiami, Himmah; Sulistyaningsih, Lulut Dwi
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.1.1012

Abstract

Calamus vitiensis, previously known to occur only in Papua New Guinea, Australia (Queensland), the Solomon Islands, Vanuatu, and Fiji is now also found in the mountainous area of Batudulang, West Sumbawa, Lesser Sunda Islands, Indonesia. This paper presents a complete report of the description, notes, and illustrations of the species.
IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM NORULAINI, N.A.R. NIK; SALLEH, B.; ISKANDAR, R.A.R.; OMAR, A.K. MOHD
BIOTROPIA No. 12 (1999)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.1999.0.12.143

Abstract

ABSTRACT Artificially inoculated asparagus tissue culture plantlets with a virulent fungus,  Fusarium proliferatum showed signs of infection as early as 4 days after inoculation. Macroscopic observations revealed presence of early symptoms such as necrotic lesions at the affected area and light microscopic examinations clearly revealed the post-penetration events that took place including the destruction of surrounding cells. However, little is known of the hyphal activity or advancement on the host's surface at the initial stage after inoculation. Scanning electron microscopic examination clearly revealed the hyphal advancement on the surface and the mode of entrance into the host tissues beneath. Four days after inoculation, the fungi proceeded to spread out from the inoculation point onto the host surface which eventually developed into a sparse network of both aerial and non-aerial hyphae. Non-aerial hyphae form a network of mycelium that adheres to the surface and it's movement appeared to be oriented towards the stomata. Hyphal penetration occurs more often through the stomata, natural openings or wounds. In some cases, the hyphae crossed over the stomatal opening without entering the host tissues. At places where the cuticle layer is absent or not well developed the hyphae successfully grew in between the epidermal cells into the tissues beneath. Key words: Tissue culture/Asparagus officinalis/shoots/Artificial inoculstion/Fusarium proliferatum.
Diversity and Vertical Distribution of Vascular Epiphytes on a Malaysian Mangrove Island Rohani, Shahrudin; Lee, Fei Lin; Yusoff, Abdul Shukor
BIOTROPIA Vol. 27 No. 2 (2020): BIOTROPIA Vol. 27 No. 2 August 2020
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2020.27.2.1182

Abstract

Many studies have attempted to explain the diversity and abundance of epiphytic plants in major ecosystems worldwide. However, investigations on the abundance of epiphytic plants in mangroves have remained rare. The aim of this research was to study the diversity and vertical distribution of vascular epiphytes in a mangrove forest in Peninsular Malaysia. Sampling was conducted over a 0.1-hectare plot on Pulau Telaga Tujuh, a mangrove island in Terengganu, Malaysia. Trees with vascular epiphytes were divided into three strata: basal, trunk, and canopy. The vascular epiphytes were identified, and the number of individuals in each stratum was recorded. In total, eight species of vascular epiphytes from six genera and four families were recorded. Pulau Telaga Tujuh mangrove forest exhibited a relatively low diversity of vascular epiphytes (H' = 1.43). The dominance of Hydnophytum formicarium significantly contributed to the diversity of vascular epiphytes in this forest. The highest abundance of epiphytes was observed on the trunks of the host trees. The vertical distribution pattern observed in this study is associated with the adaptation of epiphytic plants to stresses in the mangrove ecosystem, particularly drought and salt spray. Pulau Telaga Tujuh had a high density of vascular epiphytes but lower diversity compared with other ecosystems.
ASPERGILLUS FLAVUS INFECTION AND AFLATOXIN CONTAMINATION IN PEANUTS AT VARIOUS STAGES OF THE DELIVERY CHAINS IN WONOGIRI REGENCY, CENTRAL JAVA, INDONESIA Dharmaputra, Okky Setyawati; Retnowati, Ina; Ambarwati, Santi
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2007.14.2.15

Abstract

As a part of an Australian Centre for International Agricultural Research (ACIAR) project on management of aflatoxin in Indonesia and Australia (PHT 97/017), a survey on pre- and postharvest handling of peanuts at farmer, collector retailer levels, including Aspergillus flavus  infection and aflatoxin B1 contamination of peanuts collected in Wonogiri regency, Central Java, was conducted during the harvest period of the wet (February 2003) and dry (June 2003) seasons. Interviews using questionnaires, with farmers, collectors and retailers concerning pre- and post-harvest handling of peanuts were carried out. The moisture contents and physical quality of the peanut kernels were also determined. During the wet and dry seasons, fresh pod samples were collected from farmer fields (24 samples), dry raw pod samples from collector (24 samples) and raw kernel samples from retailer levels (54 samples). Thus, during each season 102 samples of various kinds of peanuts were collected for analysis. The A. flavus infection in kernel samples of peanuts collected from retailers were the highest, followed by samples collected from collectors and farmers. The A. flavus infection in kernel samples collected during the dry season was in general lower than in the wet season. It was alarming to note that all most all samples of raw kernel samples collected from retailers were infected by A. flavus during the wet and dry seasons. In general aflatoxin B1 levels in peanut samples collected in the wet season were higher than the dry season. During the wet and dry seasons, the aflatoxin B1 contents in retailer samples were the highest, followed by those collected from collectors and farmers. During the wet season, around 4, 17 and 33% of peanut samples collected from farmers, collectors and retailers, were respectively contaminated with more than 15 ppb of aflatoxin B1. During the dry season, around 42 and 74% of peanut samples collected from collectors and retailers, were respectively contaminated with more than 15 ppb of aflatoxin B1, while aflatoxin levels in wet raw pod samples collected from farmers were less than 15 ppb. In 2003 Codex Alimentarius Commission has determined a maximum level of total aflatoxins in peanuts intended for further processing at 15 ppb.

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