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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol. 10 No. 2 (2020)" : 11 Documents clear
Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia Mamluatul Faizah; Tri Ardyati; Suharjono Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.09

Abstract

Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82 - 1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence. Keywords: Burkholderia cepacia, cellulolytic bacteria, cellulase enzyme, sago waste.
Characterization of Probiotics Isolated from Intestine of Mackerel Fish (Rastrelliger sp.) from Lembata Regency of East Nusa Tenggara Helena Daten; Tri Ardyati; Yoga Dwi Jatmiko
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.04

Abstract

The research aimed to isolate, characterize, and analyze the ability of lactic acid bacteria (LAB) potential as probiotics to produce hydrolase enzyme. The LAB was isolated using MRS agar by the spread plate method. The LAB characterization includes antimicrobial activity, tolerance to low pH, bile salt, salinity, autoaggregation properties, and ability to produce hydrolytic enzymes. The isolate which has the highest ability to inhibit Aeromonas hydrophila is KBP 3.3, while the isolate which inhibits the highest Streptococcus agalactiae is KBP 1.1.1. The KBP 3.3 and KBP 1.1.1 were able to survive at pH 1 for 24 hours with a survival rate of 93.6% and 98.3%. The KBP 3.3 and KBP 1.1.1 are tolerant to 7.5% bile salt concentrations for 24 hours of 99.46% and 99.11%. The KBP 3.3 is tolerant to 0.5 % salinity for 24 hours with the highest survival rate of 113.38%, while KBP 1.1.1 is 94%. The KBP 3.3 and 1.1.1 have autoaggregation properties of 92.18% and 87.84%. The KBP 3.3 produced the highest lipase enzyme, while KBP 1.1.1 produced the protease enzyme.Keywords: hydrolytic enzyme, lactic acid bacteria, mackerel, probiotic
The Use of Food Coloring Dyes in Bacterial Staining Rio Risandiansyah; Arniyati Arniyati; Nofie Irmalia Nurita; Natasya Hana Gionika
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.10

Abstract

Staining creates a contrast between the cells and its surrounding, and enables the microscopic characteristics of bacterial cells to be easily visible and distinguished. However, staining often relies on dyes which are expensive, not readily available, or toxic. In this study, the use of food coloring dyes to stain bacteria was explored. We stained Gram- positive bacteria (Bacillus sp. and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) using several food coloring dyes of different colors, which were purchased locally. After slide fixation, the dye was flooded on the bacterial smear and air-dried for up to 30 minutes and observed by using microscope before and after washing with water. The results of this study show that prior to washing, most food coloring dyes were able to stain bacterial cells. However, after washing, only pink and purple food coloring dyes were retained, showing pink colored cells. We suspected that erythrosine was the agent responsible for this result, and was able to show similar characteristics with erythrosine alone. This study concludes that food coloring dyes containing erythrosine can be used to stain bacterial cells indiscriminately.Keywords: Bacterial cell staining, erythrosine, food coloring dyes, resource-limited.
Virtual Prediction of The Effect Phenolic And Glucosinolate Compounds In Broccoli (Brassica Oleracea) On Anti-aging As Stimulant Nrf-2 Viona Faiqoh Hikmawati; Fajar Mustika Alam; Jihan Shavira Ainnayah; Fatchiyah Fatchiyah
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.05

Abstract

Aging is caused by an imbalance between antioxidants and ROS. Nuclear Factor Erythroid 2-related factor 2 (Nrf2) is a transcription factor that regulates antioxidant genes. Under normal conditions, Nrf2 will bind keap1 and cause degradation of Nrf2. Nrf2 activation can be stimulated by secondary metabolites, such as glucosinolate (glucoraphanin and sulforaphane) and phenolic (kaempferol and quercetin) groups found in broccoli (Brassica oleracea). The purposes of this study were to analyze the interaction of the four compounds with Keap1 through molecular docking, to identify interactions that inhibit Keap1, and also to know the bioactivity scores, drug-likeness, and bioactivity prediction of each compound. The Nrf2-Keap1 protein (ID: 2FLU) structure was retrieved from the protein database, whereas the quercetin (CID: 5280343); kaempferol (CID: 5280863), sulforaphane (CID: 5350), and glucoraphanin (CID: 656556) were obtained from the PubChem Database. Molecular docking was done with HEX 8.0. The docking results were visualized with Discovery Studio 2020. Drug-likeness and bioactivity scores of the compounds were identified using mollinspiration. Prediction of bioactivity was carried out with PASS Online. The results showed that the binding energy of quercetin with Keap1 was -268.72 kcal.mol-1, and glucoraphanin with Keap1 was -318.01 kcal.mol-1. We found that quercetin from the phenolic group and glucoraphanin from the glucosinolate group had a strong interaction with Keap1, indicated by the number of interactions occurred and the smaller energy needed. Hence both compounds could inhibit the interaction of Keap1-Nrf2. Consequently, Nrf2 could transcribe antioxidant genes. The interaction between Keap1 and quercetin may play a role related to ROS reduction activities, such as enhancing HMOXI expression. This study indicates that quercetin has more potential in drug development as peroxidase inhibitors.Keyword: Aging, bioinformatic, glucoraphanin, keap1, quercetin
The Effect of Gamma Irradiation on the Growth and Multiplication of the In Vitro Shoot of Patchouli (Pogostemon cablin Benth.) Yunia Efrice Banyo Efrice Banyo; Serafinah Indriyani; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.11

Abstract

The objective of this research was to evaluate the effect of gamma irradiation on shoot growth and multiplication of Patchouli (Pogostemon cablin Benth.) Two weeks-old in vitro shoots were irradiated gamma-ray, at doses of 0, 15, 30, 45, 60, and 75 Gy. The control shoot was not irradiated. The irradiated shoots were cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg.L-1 NAA and 0.3 mg.L-1 BA and incubated in a growth room for eight weeks at a temperature of 25±2°C. The results showed that the gamma irradiation inhibited the growth and multiplication of shoots. Explants irradiated with high-dose gamma-ray (45-75 Gy) had not formed shoot in four weeks of culture, while 58.3-83.3% of the explants without irradiation or irradiated at low doses 15 and 30 Gy formed shoots. The higher irradiation doses increased percentage of browning explants and reduced the percentage of forming shoots. Within the eight weeks of culture, explant without irradiation was able to form shoots at the percentage of 100% with 24 shoots per explant, while explants irradiated at 15-45 Gy were able to grow form shoots at the percentage of 77.7-95.5%. The high doses-irradiated explants (60 and 75 Gy) were only able to form shoots less than 13-20%, with 2-3 shoots per explant. Keywords: Gamma rays (Gy), in vitro shoot, Pogostemon cablin Benth.
Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study Siska Nanda Widhaningrum; Septiawan Putranto; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.06

Abstract

Water clover (Marsilea crenata) is one of the herbal plants that has been using in alternative medicine. It possesses pharmacologically active compounds like flavonoid, which has cellular activities such as antioxidant and estrogenic activity. This study aimed to evaluate the impact of water clover ethanol extract (WCE) at different concentrations on the growth of follicles and oocytes based on follicles and oocytes diameter, respectively, after six days of culture. This experimental study used 24 isolated antral follicles (2.5-3.2 mm), which were randomly divided into four groups including control (without supplemented WCE) and experimental groups that supplemented with different concentrations of WCE (21.6, 43.2, and 86.4 µg ml-1) in culture medium for six days culture. The diameter of follicles was measured on days 0, 3, and 6. Additionally, oocytes diameter was also measured on day 6. The results indicate that the mean diameter of antral follicles and oocyte diameter of WCE 43.2 µg ml-1 was significantly increase compared to the other groups (P≤0.05). According to our results, WCE exerts its effect on the growth of the antral follicle and oocyte based on follicles and oocytes diameter respectively in a dose-dependent manner after six days of the antral follicle cultured.  Keywords: antral follicle, flavonoid, in vitro, oocyte, water clover.
Optimal Control of Cervical Cancer Model with Vaccination and Screening Karunia Theda Kristanti; Trisilowati Trisilowati; Agus Widodo
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.01

Abstract

In this paper, an optimal control problem of a cervical cancer model with vaccination and screening as controls is discussed. This vaccine can stimulate the immune system to produce antibodies that can prevent the occurrence of human papillomavirus (HPV) infections, while screening is used as secondary prevention of early detection of cervical cancer cells so that treatment can begin immediately. The models were divided into two compartments, females and males. The female's compartment consists of susceptible, vaccinated, infected, screening, cervical cancer, and recovered females. Meanwhile, the male's compartment consists of susceptible, infected, and recovered males. The purpose of this optimal control was to minimize the number of infected females, infected males, and cervical cancer, as well as to minimize the cost of the controls. Optimal control was obtained by using the Pontryagin principle. Furthermore, an optimal control problem was solved numerically using the Forward-Backward Sweep method to determine the effect of vaccination and screening on the model. The results indicate that vaccination and screening as controls are effective in reducing the subpopulation of HPV infection, which can further reduce the occurrence of cervical cancer. Keywords: cervical cancer, vaccination, screening, optimal control
Plant Growth Promoting Endophytic Bacteria of Coffea canephora and Coffea arabica L. in UB Forest Esti Rizkiana Pratiwi; Tri Ardyati; Suharjono Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.07

Abstract

Plant Growth Promoting (PGP) Endophytic bacteria are used as an alternative biofertilizer to support soil health and plant productivity. This research aimed to isolate, analyze the potential, and identify the endophytic bacteria of Robusta and Arabica coffee plants as biofertilizer agents. Endophytic bacteria were isolated from the roots of coffee plants and tested for their potential to produce IAA, phosphate-solubilizing, and nitrogen fixation. Potential endophytic bacterial isolates were identified based on 16S rDNA sequence similarity. Total isolates from Robusta coffee consisting of ten IAA-producing bacteria, eight phosphate-solubilizing, and seven nitrogen fixation bacteria isolates. Total isolates from Arabica coffee roots were 12 isolates of IAA-producing bacteria, seven isolates of phosphate-solubilizing bacteria, and six isolates of nitrogen fixation bacteria. The highest potential of the isolate from Robusta roots was SS.E2 isolate to produce IAA 110.73 μg.mL-1; SS.P3 isolate to dissolve phosphate 4.42 μg.mL-1, and SS.N2 isolate to produce ammonium 3.15 μg.mL-1. The highest potential of the isolate from Arabica roots was SW.E9 isolate to produce IAA up to 257.16 μg.mL-1; SW.P5 isolate to dissolve phosphate up to 4.55 μg.mL-1; and SW.N6 isolate to produce ammonium up to 1.16 μg.mL-1. Isolates SS.E2, SW.E9, SS.P3, SW.P5, SS.N2, and SW.N6 were respectively identified as Bacillus cereus ATCC 14579, Bacillus cereus ATCC 14579, Rahnella aquatilis B35, Kluyvera intermedia TPY16, Rahnella aquatilis B35, and Pseudomonas tolaasii NCPPB 2192. Potential PGP isolates can be developed as biofertilizer agents for the coffee plant. Keywords: Coffee, Endophytic bacteria, IAA, Nitrogen, Phosphate
Differential Intestinal Microbiota Composition Inhibits the Lactobacillus Growth in Rheumatoid Arthritis Patients in Malang, Indonesia. Mufidah Mufidah; Eko Suyanto; Viranda Sutanti; Hazna Noor Meidinna; Fatchiyah Fatchiyah
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.02

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease that can cause progressive damage to the joints of patients. The number of patients is expected to increase, along with the exact cause of this disease remains unknown. However, there are several risk factors associated with RA, including dysbiosis. The purpose of this study was to characterize the composition of intestinal microbiota in the RA and control groups through fecal analysis and reveal the association of microbiota composition with RA disease in Indonesia, especially Malang. Fecal samples were obtained from RA patients and controls. Fecal analysis was carried out through several stages, namely the calculation of total bacterial colonies, isolation and characterization of anaerobic bacteria, calculation of the Simpson diversity index, and DNA isolation. Analysis of bacterial composition profiles in fecal was carried out using 6 specific primer sets through PCR analysis. The results of the 16S rRNA PCR analysis showed different microbiota compositions between RA patients and controls. The number of Enterococcus bacterial group was lower in the control patients than the RA group, whereas the Lactobacillus bacteria decreased in RA patients. In addition, our study found that the existence of bacterial isolate 11 changed the composition of microbiota in RA patients, and the DNA band only appeared in Universal primers. The diversity of bacterial species can provide symbiotic and pathogenetic effects in RA patients.Keywords: Dysbiosis, intestinal microbiota, PCR, rheumatoid arthritis.
Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain Rosyi Wirastuti; Agung Pramana Warih Marhendra; Jantje Wiliem Souhaly; Sri Rahayu
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.08

Abstract

The aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0.9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and 96 h at 4°C. The data were analyzed using ANOVA (P<0.05). The results showed that the quality (motility and viability) of spermatozoa C. carpio L. punten strain in cold storage could be maintained at 96 h. The optimum storage for motility was found in diluent of egg yolk concentrate 5% at 48 h. MDA levels of semen from C. carpio L. Punten strains after being stored at cold temperatures increased at 0 h to 24 h, then decreased after 48 h of storage and increase at 72 h - 96 h stored. Egg yolk concentration and storage time had a significant effect on MDA levels. There is a negative correlation between MDA levels and motility. There was no correlation between MDA levels and the viability of spermatozoa C. carpio L. punten strain. Keywords: C. carpio L, Egg yolk, Malondialdehyde, Motiliy, Viability

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