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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Antimicrobial and Antioxidant Activity of Endophyte Bacteria Associated with Curcuma longa Rhizome Sulistiyani Sulistiyani; Tri Ardyati; Sri Winarsih
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Most cases of bacterial resistance towards antibiotics, encourage various efforts to gain new sources of antibiotics. Endophyte bacteria is a microorganism has important role as the producer of bioactive compounds. Endophyte bacteria from Curcuma longa with antimicrobial and antioxidant activities have not been studied yet. Curcuma longa has been utilized as the main ingredients of traditional herbal medicines (jamu). The objective of this research was to investigate the antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa rhizome. Based on morphological characteristics of bacterial colonies, eight endophyte bacteria was isolated from Curcuma longa rhizome. Screening of endophyte isolate has antimicrobial activity was done using agar well diffusion method. The culture supernatant of each endophyte isolate was dropped on agar well against pathogenic bacteria Salmonella enterica ser. Typhi, Staphylococcus aureus and yeast Candida albicans. Three endophyte isolates K3, K2 and M1b showed antimicrobial activity against pathogenic bacteria and yeast. Isolate K3 showed strong antimicrobial activity against C. albicans and S. aureus, however, isolate K2 and isolate M1b showed antimicrobial activity against Salmonella enterica ser. Typhi and S. aureus, respectively. Those endophyte bacteria also had antioxidant activity shown by scavenging ability toward DPPH radical with consecutive percentage of isolate K3 (72.3 %), K2 (51.3 %) and M1b (64.6 %). Isolate K3 showed the highest antimicrobial and antioxidant activity. Based on biochemical characteristics using Microbact 24E kit, isolate K3 was identified as Paenibacilus alvei and isolate K2 as Enterobacter agglomerans. Keywords: antimicrobial, antioxidant, Curcuma longa's rhizome, endophyte bacteria.

Transoceanic Disperse of the White-lipped Island Pit Viper (Cryptelytrops insularis; Kramer,1997) from Sundaland to Lesser Sunda, Indonesia Bagus Priambodo; Fu-Guo Robert Liu; Nia Kurniawan
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

White-lipped island pit viper (Cryptelytrops insularis) is one of the most distributed Viperidae in Indonesia, especially in eastern part of Sundaland and Lesser Sunda. To investigate the evolutionary history and the dispersal pattern of C. insularis, we collected 23 samples from 11 localities. Four simultaneous genes composing two mitochondrial genes (16S rRNA & ND4) and two nuclear genes (7IβFIB & 3ITBP) have been successfully amplified and sequenced. Bayesian inference was performed to reconstruct the phylogeny tree. Furthermore, time divergence and the population demography analyses were estimated. The phylogeny tree of C. insularis exhibits monophyletic group, with four geographically structured lineages. The time divergence estimation indicated that C. insularis evolved at approximately 7 million years ago (mya). Population demography was inferred by Bayesian Skyline Plot analysis, it shows that the population increased constantly from the past to recent time. The evolutionary history of C. insularis can be explained by a pattern of the time divergences estimation that indicating movement from West (Java) to East (Lesser Sunda). We expected that the dispersal factor of C. insularis into many different islands (in Lesser Sunda) is caused by the animal helped and also oceanic rafting which could be the stepping stones to another island. Keywords: Cryptelytrops insularis, dispersal patterns, phylogeny, population demography, time divergence

Analisis Sekuensing Nucleotida pada Mutasi Gen MTHFR C677T pada Kondisi Premature Cardio Infarction Muizzuddin Muizzuddin; Tinny Endang H.; I Ketut Muliartha; Liliek Sulistyowati; Djanggan Sargowo
The Journal of Experimental Life Science Vol. 2 No. 2 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Penelitian ini bertujuan untuk mengetahui miokard infark prematur yang terjadi karena homocystein disebabkan oleh adanya mutasi gen C677T MTHFR. Sampel darah diambil dari pasien dengan keadaan intermediate hyperhomocystein) sebanyak 14 orang; dua diantaranya hyperhomocystin obligat. Pengukuran DNA Total menggunakan spektrofotometer 260 nm dan 280 nm dan visualisasi menggunakan elektroforesis gel agarosa. DNA mutan gen MTHFR diamplifikasi dengan PCR dan gen MTHFR (C677T) diisolasi menggunakan primer yang dirancang menggunakan Fast PCR. Gen mutan MTHFR (C677T) diamplifikasi dan visualisasi melalui Automated Sequencer Analyzer dengan pembanding gen CBS exon 3, 7, dan 8 normal, serta data sekuens Gene Bank OMIM. Hasil penelitian menunjukkan bahwa determinasi konstruksi primer untuk lokus mutasi C677T semakin mudah ditetapkan. Sehingga membuka peluang untuk menggali informasi baru mengenai protein patologis pada pasien miokard infark prematur. Molekul polipeptida yang ditemukan memiliki sekuens GRLQLRSGPGEAHPK *VW*LL*HLCGRLPQRPPRSREL*G*PEALEGEGVCGSR FHHHAAFL*G dengan berat molekul 1.6 kda. Pencandraan molekul tersebut dengan NOC memiliki motif Glycosaminoglycan site dan N-Myristolation site, diduga dapat mempercepat proses atherosclerosis dan atherothrombosis. Kata kunci: Gen MTHFR C677t, homocystein, Premature Cardio Infarction

Effect of 17β-Estradiol on Feminization, Growth Rate and Survival Rate of Pacific White Shrimp (Litopenaeus vannamei, Boone 1931) Postlarvae I Nengah Gde Sugestya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

This study was therefore aimed to determine the effect of different concentration of estrogen hormone, 17β-estradiol (E2) on feminization for the production of all female, growth rate and survival rate of Pacific white shrimp (Litopenaeus vannamei) postlarvae (PL). L. vannamei PL1 were stocked randomly at a density of 10 ind.L-1 into a 10 L of seawater for each experimental flask with three replicates each. PL1 was immersed in seawater containing 0.5, 1.0 and 1.5 mg.L-1 estrogen hormone, E2 for 4 hours. The experiment was continued for 30 days for larval rearing. At the termination of experiment, the specimens in each treatment groups were weighed and measured individually for their wet body weight (BW) and total length (TL) for the mean weight gain, length gain, and the specific growth rate (SGR) estimation. The mean sex ratio values of male to female postlarvae obtained from control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 1:1, 1:2, 1:9 and 0:1 respectively. The weight gain and length gain were 40605% and 606% for control, 46310% and 647% for 0.5 mg.L-1 concentration, 49310% and 663% for 1.0 mg.L-1 concentration and 45048% and 628% for 1.5 mg.L-1. The mean SGR BW and SGR TL for control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 20.00 and 6.51, 20.46 and 6.70, 20.67 and 6.77 and 20.37 and 6.62 respectively. The mean survival rate for E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 52.67%, 54.33%, 54.67% and 52.33% respectively. The study shows that while the concentration of E2 hormone increased, the female sex ratio of L. vannamei PL also increased but the growth rate and survival rate decreased at concentration of 1.5 mg.L-1. Thus the optimum concentration of this hormone usage was 1.0 mg.L-1 immersed for 4 hours. Keywords: 17β-estradiol, feminization, growth rate, Litopenaeus vannamei, postlarvae, survival rate.

Pengamatan Jaringan Lambung Kijing Taiwan (Anodonta woodiana Lea) Yang Terdedah Pestisida Diazinon 60 EC Pada Beberapa Konsentrasi Listiya Gita Lesmana; Diana Arfiati; Asus Maizar
The Journal of Experimental Life Science Vol. 3 No. 1 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

Jaringan lambung yang diamati berasal dari kijing (Anodonta woodiana) yang terdedah pestisida Diazinon 60 EC selama 2 minggu dengan pemberian dosis yang berbeda, yaitu 3,5x10-6 ; 1,75x10-6 ; 0,875x10-6 dan 0 ppm. Kondisi kijing diamati sebelum pemberian pestisida dan setelah 1 minggu dan 2 minggu pendedahan. Irisan lambung dibuat dari sampel 1 dan 2 minggu pendedahan menggunakan pewarnaan HE. Analisis histologi lambung kijing menunjukkan bahwa dinding lambung setelah terdedah pestisida akan mengalami kerusakan berupa penebalan dinding (edema), dengan jarak digestive divericula saling berjauhan, silia dalam lambung terlihat menyatu (fusi) dan terjadi pembengkakan silia (hyperplasia). Bahkan setelah pendedahan pestisida selama 2 minggu, dinding lambung makin menebal serta digestive diverticula mengalami pengerutan (atropi) dan pemecahan atau kerusakan sel (nekrosis).Kata Kunci : Anodonta woodiana, Diazinon 60 EC, Kerusakan lambung

Modified Risk Factors for Coronary Heart Disease (CHD) in Minahasa Ethnic Group From Manado City Indonesia Ester Jeini Nelwan; Edi Widjajanto; Sri Andarini; M. Sasmito Djati
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Coronary heart disease (CHD) is a non-transmitted disease which is particular concern at the global, national, and local level. CHD has become a concern because it has caused a lot of deaths. Risk factors linked with CHD consist of permanent risk factors and variable risk factors. The purpose of the research is to analyze the correlation between variable risk factors and CHD in the Minahasa ethnic society in Manado City. This research was an observational case-control study conducted by Prof. dr. R. D. Kandou Hospital, Manado, from August to October 2016. In total, there were 220 patients included. The sampling used a simple random sampling method and the data obtained were analyzed by chi-squared test. According to the results, hypertension, smoking, and behavioral type were connected to cases of CHD. The correlation degree showed that the respondents suffering hypertension were 5.70 times more likely to suffer CHD, the smoking respondents were 2.25 times more likely to experience, and behavior type A respondents were 2.96 times more likely to suffer CHD. Hypertension, smoking, and behavioral type are linked with CHD, so there should be some promotion and preventive actions from the health governmental institution to society, especially aimed at adults, about the quality of life enhancement by healthy behavior and avoiding CHD risk factors. Keywords: behavioral type, Case-control study, hypertension, smoking behavior.

Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam Fitri Sil Valen; Maheno Sri Widodo; Yuni Kilawati
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%.Keywords: DNA barcode, Molecular identification, Mystacoleucus, Phylogenetic COI.

EMSA Eritin Blocks Nuclear Factor-Kappa B in Diabetes Mellitus Mice Model Maic Audo Lin Sihombing; Sutiman B. Sumitro; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Diabetes mellitus is one of the common metabolic disorders with increasing prevalence during recent years with hyperglycemia as its characteristic. DM has been shown to be a state of free radicals over production resulted from hyperglycemia that can activate cellular signaling pathways transcription of factor NF-κB which stimulates the production of several inflammatory mediators andl lead to chronic inflammation. Chronic inflammation is implicated in β-cell damage and function and promotes apoptosis. EMSA Eritin is a polyherbal consisting of soy bean extracts, coconut water extract and red rice extract that assumed to be antidiabetic and anti-inflammatory. This study will assess the effectiveness of EMSA Eritin against inflammation in diabetes mellitus by measuring levels of NF-κB produced by immunocompetent cells in DM mice model. Streptozotocin 100 mg.kg-1 BW is used to induce diabetes mellitus in mice. Oral administration of EMSA Eritin was given for 14 days with dose of 0.3125 mg.g-1 BW, 3.125 mg.g-1 BW and 31.25 mg.g-1 BW. Data were analyzed using One-way ANOVA (p<0.05) and Duncan test using SPSS 16.0 for Windows. The results showed that EMSA Eritin can be used as an alternative therapy for the treatment of DM. The level of NF-κB in diabetic mice significantly decreased when the mice received EMSA Eritin. Keywords: Diabetes Mellitus, EMSA Eritin, NF-κB, ROS

Dynamical Analysis of Predator-Prey Model Leslie-Gower with Omnivore Rina Exviani; Wuryansari Muharini Kusumawinahyu; Noor Hidayat
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

This article discussed a dynamical analysis on a model of predator-prey Leslie-Gower with omnivores which is modified by Lotka-Volterra model with omnivore. The dynamical analysis is done by determining the equilibrium point with its existing condition and analyzing the local stability of the equilibrium point. Based on the analysis, there are seven points of equilibrium. Three of them always exist while the four others exist under certain conditions. Four points of equilibrium, namely andÂ are unstable, while the others three equilibrium point are local asymptotically stable under certain conditions. Moreover, it's also conducted numerical simulations to illustrate the analytical. The results of numerical simulations agree with the results of the dynamical analysis. Keywords: local stability, omnivore, predator-prey models, the equilibrium point

Regenerasi Kalus Berfilamen Rumput Laut Kappaphycus alvarezii Pada berbagai Perbandingan Zat Pengatur Tumbuh Auksin (Indole Acetic Acid) dan Sitokinin (Kinetin, Zeatin) Sri Redjeki Hesti Mulyaningrum; Andi Parenrengi; Yenny Risjani; Happy Nursyam
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Interaksi auksin-sitokinin dianggap penting untuk mengatur pertumbuhan dan perkembangan dalam jaringan tanaman. Penelitian ini bertujuan untuk menentukan komposisi auksin dan sitokinin yang tepat untuk regenerasi filamen kalus rumput laut K. alvarezii. Kultur filamen kalus dilakukan pada media cair dengan formulasi ZPT indole acetic acid (IAA) : kinetin : zeatin, dengan komposisi konsentrasi sebagai berikut : A (0,4:0:1) ppm; B (0,4:0,25:0,75) ppm; C (0,4:0,5:0,5) ppm; D (0,4:0,75:0,25) ppm; E (0,4:1:0) ppm; kontrol (tanpa ZPT). Penelitian menggunakan rancangan acak lengkap dengan pengulangan masing-masing perlakuan 3 kali. Parameter yang diamati adalah laju pertumbuhan harian, sintasan, kecepatan regenerasi, panjang tunas dan perkembangan morfologi. Hasil yang diperoleh menunjukkan bahwa formula ZPT terbaik adalah formula A dengan laju pertumbuhan harian 1,929%/hari, sintasan 83,33%, kecepatan regenerasi 41,67% dan rata-rata panjang tunas 44,59 µm. Tunas mulai terbentuk pada 15 hari masa kultur. Kata kunci: formula zat pengatur tumbuh, auksin-sitokinin, K. alvarezii, mikropropagasi

Page 14 of 30 | Total Record : 299

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Home Page

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

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Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA