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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Efek Konsentrasi Sublethal Fenol Terhadap Total Haemocyte Count (THC) dan Histologi Insang Kepiting Bakau (Scylla serata) Alfi Hermawati Waskita Sari; Yenny Risjani; Agung Pramana Warih Mahendra
The Journal of Experimental Life Science Vol. 2 No. 2 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Hadirnya fenol yang melebihi batas ambang ke dalam ekosistem perairan dapat menjadi stresor kimia bagi organisme akuatik, termasuk juga bagi ekosistem muara oleh karena muara (estuaria) merupakan daerah pertemuan antara air tawar dari perairan sungai dan air laut sehingga berpotensi mengandung bahan kimia antropogenik. Kepiting bakau (Scylla spp.) memiliki siklus hidup yang sebagian besar berada pada ekosistem mangrove dan umum digunakan dalam studi ekotoksisitas. Total Haemocyte Count (THC) dan histologi organ insang dapat menginformasikan perubahan histologi akibat stressor oleh karena paparan toksik, terutama fenol. Perlakuan sublethal fenol dengan konsentrasi yang berbedaÂ terhadap kepiting bakau (Scylla serata) pada hari ke-1, hari ke-3, hari ke-5 dan hari ke-8 tidak terdapat perbedaan yang signifikan (p> 0,05) (Lampiran) terhadap rata-rata Total Haemocyte Count (THC). Namun pada hari ke-7 menunjukkan penurunan jumlah THC pada tiap perlakuan bila dibandingkan dengan kontrol (tanpa perlakuan). Perubahan histologi yang terjadi meningkat sebanding dengan konsentrasi fenol yang diperlakukan terhadap kepiting bakau (S. serata). Perubahan histologi yang terjadi adalah kerusakan struktur dari lamela insang (l) dan bagian terluar sinus lamela atau outer lamellar sinuses (ols) yang meliputi infiltrasi hemosit, hiperplasia maupun nekrosis.Kata Kunci : fenol, hepatopankreas, kepiting bakau.

The Effect of Explant Types and Kinetin Concentration on In Vitro Callus Induction in Vetiveria zizanioides (L.) Nash Arbaul Fauziah; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

The aims of this research were to evaluate the effect of explant types and several kinetin concentrations on in vitro induction and growth of callus vetiver (Vetiveria zizanioides (L.) Nash). Crown and tiller of vetiver were cultured on Murashige and Skoogâ€™s (MS) media supplemented with combination of 2,4-D 0.75 ppm and several kinetin concentrations (0, 0.3, 0.5, 0.75, and 1) ppm. The induction and growth of callus were influenced by type of explant and concentration of kinetin. Formation and growth of callus on tiller explant were faster than crown explant. Callus on tiller explant were formed one week after culture, while callus from crown explant were formed at four weeks after culture. Callus growth on tiller explant also was better than crown explant. Eight weeks after culture, callus fresh weight from tiller explant was 0.35 Â± 0.09 g, while callus fresh weight from crown explant was only 0.16 Â± 0.08 g. The addition of kinetin in the medium combined with 2,4-D was able to increase callus growth and the optimum concentration of kinetin used was 0.5 ppm. The addition of kinetin more than 0.5 ppm in the medium decreased the callus fresh weight.Keywords: callus, crown, in vitro culture, tiller, Vetiveria zizanioides.

Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen Rochmatika, Lailiyavina; Widyarti, Sri; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

The carcinogenic compounds such as DMBA known for having the ability to promote the DNA damage that affects the growth regulator genes. Therefore, uncontrolled cell growth will occur and change the immune system. Elephantopus scaber extract is considered as an alternative way of handling breast cancer since it capable to affect the work of the immune system. Hence, this study aims to determine the effect of E. scaber extract on quantitative changes of CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFNγ+ and CD4+TNF-α+ after induction of DMBA and estrogen. In vivo experiments were carried out by using 5-6 weeks old of female mice BALB/c strain. The extract of E. scaber leaf powder was obtained from Material Medica Batu, Malang. The experimental animals were divided into five groups such as negative control group (K-), positive control group (K+), mice treated with E. scaber extract for a week (group 1), mice treated with E. scaber extract for 2 weeks (group 2), and mice treated with E. scaber extract for 3 weeks (group 3). Induction of DMBA and estrogen was conducted by injection of DMBA (7.12 dimetylbenz (α) anthracene) 0.56 mg.kgBW-1 and estradiol hormone 0.0504 mg.kgBW-1. Mice were treated with E. scaber extract with a dose of 50 mg.kgBW-1. Lymphocytes were isolated from spleen and observed the CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFN-γ+ and CD4+TNFα+ T cells through flow cytometry analysis. One-way ANOVA (p <0.05) and SPSS were used to analyze this data. The results showed that the induction of DMBA and estradiol hormone affected mice fur loss. It also decreased the immune system by lowering the number of CD4 and CD8 T cells. However, E. scaber extracts increased the number of pro-inflammatory cytokines TNF-α. Furthermore, the DMBA and estradiol induction also promoted the number of CD4+IFN-γ+ in the first week, but decreased the number of CD4+IFN-γ+ at weeks 2 and 3, then increase the number of CD4+IFN-γ+. So, the treatment of E. scaber extracts demonstrated the ability to restore the homeostasis of the immune system in the first and second weeks. Keywords: CD4+, CD8+, DMBA, estrogen, E.scaber, IFN-γ+, TNF-α+

Hambatan EGCG terhadap Ekspresi Vascular Endothelial Growth Factor (VEGF) dan Ekspresi Vascular -endothelial Cadherin (VE-Cadherin) dalam Vaskulo-angiogenesis Embrio ayam Indriati Dwi Rahayu; Mochammad Sasmito Djati; Mohammad Rasjad Indra
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Keamanan konsumsi EGCG terhadap kehamilan, terutama dampaknya terhadap perkembangan embrio sejauh ini belum diketahui. Penelitian ini bertujuan untuk mengetahui pengaruh EGCG terhadap mekanisme vasculo-angiogenesis pada embrio ayam secara in ovo. Telur tetas diinjeksi dengan EGCG sebelum diinkubasi. Kemudian survival rate embrio, ekspresi VEGF dan ekspresi VE-Cadherin pada embrio dibandingkan dan dievaluasi. Dalam penelitian ini, nampak adanya penurunan survival rate embrio, ekspresi VEGF dan VE-Cadherin, sesuai dengan peningkatan dosis paparan EGCG. Hasil tersebut menunjukkan bahwa EGCG dapat mempengaruhi pertumbuhan embrio ayam, melalui hambatan terhadap ekspresi VEGF dan VE-Cadherin. Kata Kunci: EGCG, embrio, angiogenesis, VEGF, VE-Cadherin

The Analysis of Hepatopancreas Histologycal Damage in Neocallichirus karumba (Poore and Griffin) Shrimp Caused by Heavy Metal Pb Exposure in Madura Strait Maria Kristiani; Endang Yuli Herawati; Uun Yanuhar
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Madura strait known as the second busiest shipping lanes in Indonesia. Human activities on the environment can influence the marine ecosystem derived from the household, fishery processing and capture fisheries. It can pollute coastal environments, one of which is heavy metal exposure such as Cd, Hg, Ca, As and Pb. These metals are greatly impacting the life of sea biota. The objective of this research, therefore, is to examine the hepatopancreas damage rate of shrimp Neocallichirus karumba due to the exposure of heavy metal Pb in Madura Strait. Method of research is by analyzing Pb content in water, sediment and shrimp by taking sample from 3 different stations. Hepatopancreas damage is then analyzed with SEM-EDX. Result of research indicates that at Station A, Pb pollution in water is the biggest and counted for 0.25 ppm, while those in sediment and shrimp are 5.85 ppm and 1.24 ppm. At Station B, Pb pollution in water is 0.19 ppm, whereas that in sediment is 5.51 ppm, but that in shrimp is 1.04 ppm. At Station C, Pb pollution in water counts for 0.18 ppm, and in sediment, it stands for 5.5 ppm but 0.02 ppm for shrimp. Result of analysis against hepatopancreas damage is explained as follows. At Station A, vacuolization is 20 % and Pb content in organ is 0.520 ppm. At Station B, the parameters are 10% and 0.196ppm. At Station C, it includes 15% and 0.173ppm. Organ damage is straightforwardly related to Pb content in water and sediment. Shrimp age is quite influential to the percentage of organ damage.Keywords: Heavy metal Pb pollution, hepatopancreas organ damage, Madura Strait, N. karumba shrimp.

Isolation and Identification of Antagonistic Bacterium against a Pathogens of Bacterial Tuber Rot of Amorphophallus muelleri Nurfitri Arfani; Rodiyati Azrianingsih; Suharjono Suharjono
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Rhizosphere bacteria have the ability to protect the host plants from the infection of pathogenic microorganisms. This study aimed to identify rhizosphere bacteria that were capable of inhibiting the growth of bacterial isolates that cause tuber rot of Amorphophallus muelleri. Rhizosphere bacteria were isolated using Nutrient Agar medium by pour plate method. Isolates were subjected to antagonistic assay against several bacterial isolates from the rotten tuber of A. muelleri using dual culture method. The potential isolate was identified based on 16S rDNA sequence. Isolate R7 showed the strongest inhibition to the growth of bacterial isolates from rotten tuber with an inhibition zone diameter of 19.66 mm. The 16S rDNA sequence of isolate R7 R7 was 99.7% similar to Delftia tsuruhatensis PCL1755. The isolate was potential to be developed as phytopathogen control agent.Keywords: Amorphophallus, antagonistic bacteria, rhizosphere bacteria, rotten tuber, 16S rDNA.

Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells Aisyah Zahroh Aden; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

This experiment was aimed to determine the bioactivity of ethanolic extract of propolis (EEP) against the changes in the quantity of CD4+CD25+ and B220+ lymphocytes and determine the optimal dose of EEP to increase the number of CD4+CD25+ and B220+ cells in Balb/c mice. Balb/c mice were divided into four treatment groups: control treatment, treatments of EEP at a dose of 50 mg.kg-1, 100 mg.kg-1, and 200 mg.kg-1 body weight of mice. All mice were dissected after two weeks post treatment. Profiles of lymphocytes from the spleen expressing CD4+CD25+ and B220+ cells were analyzed by flowcytometry using CellQuest software. Data was analyzed by Kruskall Wallis and Mann Whitney statistical test with P<0.05 using SPSS 16.0 for Windows. The results showed that the treatment of dose of 50 mg.kg-1 of EEP can increase relative number of CD4+CD25+ cells significantly, but those cells decrease significantly when we apply the dose of 100 and 200 mg.kg-1. The relative number of B220+ cells increase in the dose of 50 and 200 mg.kg-1 and decrease in the dose of 100 mg.kg-1 compared to the control. This experiment suggest that EEP has bioactivity to modulate the quantity of CD4+CD25+ and B220+ in dependent manner.Keywords: B220+, CD4+CD25+, lymphocyte, propolis

Study of Glucosamine Production from Shrimp Shells by Fermentation Using Trichoderma harzianum Hardoko Hardoko; Bambang B. Sasmito; Yunita E. Puspitasari; Hamid M. Afandi; Nugroho Maulia
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Shrimp shells are one of chitin sources in Indonesia which is potential to be converted into multifunctional glucosamine. This research was aimed to study the glucosamine production by fermentation using Trichoderma harzianum.Â Method used was experimental fermentation with pH treatment of 3-5 and fermentation duration of 10-20 days, and designed using Response Surface Methodology (RSM). Results showed that fermentation duration of 10-20 days has reached the optimum point of glucosamine production from shrimp shells using T. harzianum. The highest production of D-glucosamine in fermentation using T. harzianum occurred on initial pH of 5.41 and fermentation duration of 15 days (18,294.95 ppm), while the highest N-Acethyl-D-Glucosamine production occurred on initial pH of 3.00 and fermentation duration of 20 days (127,000.00 ppm).Keywords: fermentation, glucosamine, shrimp shells, T. harzianum.

Effect of Pb and Cd Elicitors on Growth and Content of Vetiver Oil Adventitious Root In Vitro of Vetiver (Vetiveria zizanioides L. Nash) Ivani, Trecy; Widoretno, Wahyu
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

This research aimed to evaluate the effect of Pb and Cd elicitors on root growth and essential oil content of vetiver oil on the root culture of vetiver (Vetiveria zizanioides L. Nash). Roots were induced by culturing of the shoot on MS media supplemented with 0.1 mg.L-1 kinetin and 7 mg.L-1 NAA. Elicitation was done by culturing roots on MS media with 0.1 mg.L-1 kinetin and 7 mg.L-1 NAA + 0.1 mM Pb or Cd. Roots then observed for fresh weight, dry weight, number, and root length, and vetiver oil contents were analyzed using GC-MS. The addition of Pb and Cd heavy metal elicitor affected the formation and the content of vetiver oil compounds of root culture. Pb elicitor increased the number of roots, while Cd elicitor increased root length. However, the addition of Pb and Cd elicitor on culture media decreased the fresh weight and root dry weight. Fifty eight vetiver oil compounds in adventitious roots of vetiver plants were identified. The main compounds of vetiver oil in roots culture were Khusimone (6.94%), Khusimol (6.05%), Khusimene (4.85%), α Vetivone (3.70%), β Vetivone (3, 53%), Vetiverol (3.22%), Prezizaene (2.35%), and Zizaene (1.91%). Elicitation with Pb and Cd increased the composition of the main compounds of vetiver oil. Cd elicitor increased the composition of the main compound vetiver oil higher than the Pb elicitor. Keywords: adventives root, elicitor, in vitro, Pb and Cd, vetiver oil

Kajian Penambahan Dosis Beberapa Pupuk Hijau dan Pengaruhnya Terhadap Pertumbuhan Tanaman Selada (Lactuca sativa L.) Yuni Agung Nugroho; Yogi Sugito; Lily Agustina; Soemarno Soemarno
The Journal of Experimental Life Science Vol. 3 No. 2 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

Penambahan pupuk hijau pada budidaya selada anorganik bertujuan mendapatkan dosis beberapa pupuk hijau yang dapat meningkatkan pertumbuhan selada dan meningkatkan kesuburan tanah. Penelitian menggunakan Rancangan Acak Kelompok diulang tiga kali. Pemberian pupuk urea dengan dosis 100 kg.ha-1 sebagai kontrol. Perlakuan penambahan pupuk hijau adalah penambahan Tithonia 5 ton.ha-1 (OT1), 10 ton.ha-1 (OT2), Tithonia 15 ton.ha-1 (OT3); penambahan Gliricidia 5 ton.ha-1 (OG1), 10 ton.ha-1 (OG2), 15 ton.ha-1 (OG3); penambahan Cromolaena 5 ton.ha-1 (OC1), 10 ton.ha-1 (OC2), dan 15 ton.ha-1 (OC3). Pengamatan tanaman selada meliputi Bobot Segar Tanaman, Bobot Kering Tanaman, Indeks Luas Daun, Laju Pertumbuhan Tanaman, dan Laju Asimilasi Bersih. Pengamatan tanah meliputi C organik tanah, N total, KTK, pH, Bobot Isi Tanah dan CN rasio. Analisa ragam dilakukan terhadap peubah pengamatan tanaman selada dan kesuburan tanah, uji Tukey dan uji kontras dilakukan bila ada pengaruh nyata perlakuan terhadap peubah pengamatan. Uji regresi linier berganda dilakukan terhadap sekumpulan peubah kesuburan tanah terhadap hasil tanaman selada. Hasil menunjukkan bahwa penambahan Tithonia, Gliricidia dan Cromolaena sebesar 5 ton.ha-1 pada tanaman selada yang dipupuk urea meningkatkan indeks luas daun, bobot segar dan kering tanaman, dan laju pertumbuhan tanaman. Bobot segar tanaman selada tertinggi sebesar 17,18 ton.ha-1 dicapai pada penambahan Tithonia 15 ton.ha-1 yang berarti meningkat 88% dari pemupukan urea. Penambahan pupuk hijau Tithonia, Gliricidia dan Cromolaena sebesar 5 ton.ha-1 pada budidaya selada meningkatkan KTKÂ dan C organik tanah setelah panen. Residu C organik tanah tertinggi 2,97% meningkat 53,1% dan KTK tanah tertinggi 43,24 me.100g-1 meningkat 14,75% dari kontrol oleh penambahan Cromolaena 15 ton.ha-1. KTK tanah, N total, Bobot Isi tanah, dan pH tanah berturut-turut mempunyai pengaruh positif dan nyata terhadap bobot segar tanaman saat panen.Kata kunci: Pupuk hijau, pupuk buatan, tanaman selada.

Page 13 of 30 | Total Record : 299

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Jehan Ramdani Hariyati

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA