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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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The Effect of Electroporation Method towards the Motility and Viability of Java Barb Fish (Puntius javanicus) Sperm Dimas Adetia Rikianto; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Electric shock treatment of Java Barb Fish (Puntius javanicus) sperm using electroporation method on sperm as transfer gen (Sperm Mediated Gen Transfer) has not been implemented in Indonesia. This study was conducted to know the effect of electric shock using gene pulser at different voltage level toward motility and viability of Java barb fish sperm. This research was conducted at Fish Breeding Laboratory, Faculty of Marine and Fisheries and LSIH of Brawijaya University-Malang in May 2017. The trial design used Complete Randomized Design with 3 different treatments and 1 control which each treatment repeated 3 times. The treatments used the Gene pulser with 3 different voltages: A (20 V), B (30 V), C (40 V). The result showed that the electric shock treatment with different voltages level affected motility and viability of Java barb fish sperm. Based on the data analysis used polynomial orthogonal, a linear-form of the relationship among the treatments in the form of equation was found (Puntius javanicus) with R2=0.9815 and equation y = -40.5x + 130.83 with R2= 0.8626. Based on the result of this research, electroporation for Java barb fish' sperm as gene transfer media should be done with voltage 20 V, pulse number 4 times and pulse length 1 ms. Keywords: electroporation, motility, Puntius javanicus, sperm, viability.

Growth and Development of Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae) on White, Red and Black Rice Dewi Fajarwati; Ludji Pantja Astuti; Toto Himawan
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Rhyzopertha dominica is a polyphagous stored pest to many grains including rice. R. dominica can easily spread from one place to another to infest stored products. The aim of this research was to know the influence of three grains types with different weight to R. dominica growth, development, net reproduction rate and gross reproduction rate on red, black and white rice. The research was conducted in Pest Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya Malang. The research result showed that there was an interaction among three grains types with different weight to the number of R. dominica larval, pupal, and newly adult emerged, while the number of eggs, weight of newly adult emerged, the reduction percentage of grain and phase of the egg, larval, pupal and the life cycle period of R. dominica were affected by different grains types factor. The R0value showed that on the next generation, number of female was increase 1.25 times from the previous generation on white rice, while on red and black rice the population was increase up to 2.63 and 2.49 times from the previous generation.Keywords: Growth and Development, Reproduction Rate, Rice, Rhyzopertha dominica.

Bioactivity of Sauropus androgynus and Elephantopus scaber to CD4+IL2+ and CD4+IL4+ T Cells Modulation in Balb/c Pregnant Mice Model of Typhoid Yayu Tsamrotul Fuadah; Moch. Sasmito Djati; Sri Widyarti
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Pregnant woman have higher risk to get infection, because pregnancy decreasing the cell T activity. Sauropus androgynus and Elephantopus scaber has substance like saponin and flavonoid which has been well known as natural imunomodulator, particularly to increase amount of immunocompetent cell. This research is important to recognize effective supplement supply for immunomodulator of S. androgynus and E. scaber to increase mice's (Mus musculus Balb/c) immune system. This research conducted in seven treatments by 3 repetitions for each treatment by using pregnant mice which has been infected by bacteria Salmonella typhimurium (dose 107 CFU.mL-1). Bacteria are injected to mice intraperitoneal in day 5th after giving combination of extract E. scaber and S. androgynus. The dose of E. scaber and S. androgynus combination are 200; 150:37.5; 100:75; 50: 112.5; 150. Five group of treatment were infected by S. typhimurium. Two other groups were the control, namely negative control which was only given NaCMC 0.05% without infection and positive control which was given NaCMC 0.05% and infected by S. typhimurium. After being injected, treatment was redone till the day of surgery. The surgery was executed in day 12th and 18th of pregnancy. Data were analyzed using ANOVA (p < 0.05) and Duncan test. Result indicated that extract of S. androgynus and E. scaber could increase amount of immune system in pregnant mice. This was indicated from significant increasing in amount of cell T CD4+IL2+ and CD4+IL4+ in pregnant mice which has been infected by S. thypimurium. Formula of extract S. androgynus and E. scaber which could return immune condition was approached condition of healthy pregnant mice such as E. scaber 200 mg.kg-1 BW; E. scaber 100 mg.kg-1 BW and S. androgynus 75 mg.kg-1 BW; E. scaber 50 mg.kg-1 BW and S. androgynus 112.5 mg.kg-1 BW; and S. androgynus 150 mg.kg-1 BW, respectively. Keywords: CD4+IL2+,CD4+IL4+, E. scaber, Immunomodulator, S. androgynus, S. typhimurium

Rosella Flower Extract Prevent Increasing of Interleukin-6 and Amyloid-β Levels in Brain Tissue of Heated Diet-Treated Rats Ardhiyanti Puspita Ratna; Setyawati Soeharto; Edi widjajanto; Silvy Amalia Falyani; Pia Bataif Batmomolin
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Heated food is a source of formation of various types of Advanced Glycation Endproducts (AGEs). One of which is N-carboxymethyl-lysine (CML) that is often used as a sign of the formation of AGEs in the diet. The accumulation of AGEs in the brain can trigger neurodegenerative conditions. It also accelerates the accumulation of Amyloid β and proinflammatory cytokines which exacerbate neurodegeneration. Hibiscus sabdariffa L or rosella known to have antiglycation, antioxidant, and anti-inflammatory effects. This study aims to prove the effect of food heating to the increasing levels of CML serum and evaluate the effects of daily intake of Rosella extract IL-6 levels and Amyloid β in the Wistar rats' brain given a diet high of AGEs. This experimental study with post test only control group design was conducted using 25 white male rats Wistar strain (Rattus novergicus) which were divided into 5 groups namely positive control, negative control, the treatment group with rosella dose 200mg.kgBW-1, 300mg.kgBW-1, and 400mg.kgBW-1. The high diets of AGEs are given for 12 weeks, and it was added to the rosella extract with various doses on the 9th to 12th weeks. The examination of CML serum level, interleukin 6 brain level, and Amyloid β of the brain was carried by ELISA. This study found differences between CML serum level of rats which fed a standard diet and the one that was given a heated diet (p= 0.0001). It also found that rosella flower extract can prevent the increasing levels of interleukin 6 and Amyloid β in the brain tissue of rats which were given a heated diet, and both effective doses were on 200mg.kgBW-1. Keywords: Advanced Glycation End products, Amyloid β, Interleukin 6, N-carboxymethyl-lysine, Rosella.

Histologi Organ Hepatopankreas Kepiting Bakau (Scylla serata) pada Konsentrasi Sublethal Fenol sebagai Peringatan Dini (Early warning) Toksisitas Fenol di Estuaria Alfi Hermawati; Yenny Risjani; Agung Pramana Warih Mahendra
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Fenol dan senyawa fenolik merupakan salah satu xenobiotik yang menjadi salah satu faktor stres lingkungan pada biota yang terpapar dan telah menjadi masalah lingkungan akibat dampak antropogenik lingkungan. Konsentrasi fenol yang melebihi batas ambang ke dalam ekosistem perairan dapat menjadi stresor kimia bagi organisme akuatik, termasuk kepiting bakau (Scylla serata). Teknik histologi juga merupakan salah satu metode umum yang dapat digunakan untuk mengetahui efek sublethal polutan. Sebelum menetapkan konsentrasi sublethal, terlebih dahulu dilakukan penentuan konsentrasi LC50 (96 jam). Berdasarkan data mortalitas dari hasil uji toksisitas akut LC50 (96 jam) fenol terhadap kepiting bakau (Scylla serata) yang dianalisis dengan analisis probit, diperoleh konsentrasi LC50 (96 jam) sebesar 26 mg.L-1. Dari hasil tersebut kemudian ditentukan perlakuan konsentrasi sublethal fenol (96 jam) yang dilakukan, yakni perlakuan A (1,62 mg.L-1), B (6,5 mg.L-1), C (13 mg.L-1) serta kontrol. Hasil pengamatan pada akhir perlakuan diketahui terdapat perbedaan yang signifikan terhadap histologi organ insang dan hepatopankreas pada tiap perlakuan dimana pada konsentrasi yang semakin tinggi menunjukkan kerusakan jaringan yang semakin terlihat jelas pada hepatopankreas juga terlihat perubahan yang signifikan yang terlihat pada tubulus hepatopankreatik kepiting bakau (S. serata) yaitu vakuola lebih terbentuk, epitel tampak tidak teratur, hilangnya bentuk bintang pada lumen. Kata Kunci : fenol, hepatopankreas, kepiting bakau.

The Effect of Organic Stimulant and Inorganic Fertilizer on Two Rice Varieties (Oryza sativa L.) Erningtyas Widyaswari; Mudji Santosa; Moch. Dawam Maghfoer
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Increased the yield of rice could be done by organic stimulant application and inorganic fertilizer, as well as the used of appropriate variety. The field experiment was aimed to study the interaction of fertilizer application and the using of variety on rice. The research was conducted at April until July 2016 in Sekarputih Hamlet, Pendem Village, Junrejo District, Batu City. This research used the Randomized Complete Block Design (RCBD) Factorial methods with 2 factors are Rice Variety (V) and Fertilizers Doses (P) with 3 replications. Cows Biourine application by dissolved 1 L biourine with 10 L water sprayed on soil and plants. EM-4 application was doing by dissolved 100 cc EM-4 with 10 L water sprayed on soil. The result of research showed that interaction of Mapan-P.05 hybrid variety (V2) added with fertilizer doses 100 kg phonska+100 kg urea+cows biourine+EM-4 (P6) can increase yield on rice in parameter 1000 grain weight to 15.29% against which added fertilizer doses 200 kg phonska+200 kg urea (P1). Keywords: Inorganic Fertilizer, Organic Stimulant, Rice, Variety.

The Effect of Monosodium L-Glutamate (MSG) Treatment for Short and Long Terms to The Semen Quality of Adult Male Rats Ivakhul Anzila; Agung Pramana Warih Marhendra; Sri Rahayu
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

This study was conducted to investigate the effect of MSG treatment for short and long term on the semen quality of adult male rats. Twelve male adult Wistar rats with 200-300g of body weight (BW) and 3-4 month of age were used in this study. The animals were divided randomly into 3 groups. M0 was used as a control, M1 and M2 were given with MSG 4mg/gBW for 15 andÂ 45 days respectively. The experimental animals were sacrificed on the days 16th (to M1 group) and 46th (to M0 and M2 groups). The epididymis was isolated and semen quality (motility, viability, concentration, and abnormality of sperm) was evaluated. The results showed motility and concentration of M1 and M2 were not significantly decreased compared to M0. MSG treatment also significantly reduced viability and increased abnormality of sperm. Analysis of sperm abnormality character shows that the use of long-term MSG caused a formation of the primary abnormality (round and double head sperm) and increased the secondary abnormality (bent neck, curve tail, coiled tail, headless, and tailless) compared to control. Conclusion, semen quality decreases with consumed MSG for the long term. For this reason, reconsidering the use of MSG as an enhancer for the teste of food is very important.Keywords: epididymis, Monosodium L-Glutamate, semen quality.

Identifikasi Molekul Adhesi Pili Pseudomonas aeruginosa pada Human Umbilical Vein Endothelial Cells (HUVECs) Culture Dwi Yuni Nur Hidayati
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Pseudomonas aeruginosa merupakan salah satu penyebab Gram negatif bakteriemia yang bisa berlanjut menjadi sepsis. Bakteri ini banyak menginfeksi penderita di rumah sakit dengan membentuk koloni pada pembuluh darah melalui proses adhesi (pelekatan). Pili dan bagian Outer Membrane Protein (OMP) adalah faktor yang mempengaruhi pelekatan bakteri P. aeruginosa. Tujuan dari penelitian ini adalah untuk mengetahui berat molekul protein hemaglutinin yang terdapat pada pili dan membuktikan peran protein hemaglutinin pada pili dalam pelekatan bakteri P. aeruginosa. Metode yang digunakan dalam penelitian ini adalah isolasi protein pili P. aeruginosa secara bertingkat, dilanjutkan dengan uji hemaglutinasi (metode mikrotiter) dan uji adhesi menggunakan protein pili hasil elektroelusi yang disalutkan pada kultur sel endotel (HUVECs) (konsentrasi 1, ½, ¼, ⅛, ⅟16, dan 0 [kontrol]). Hasil penelitian menunjukkan adanya reaksi hemaglutinasi pada potongan pili ketiga dengan titer tertinggi (1/128). Protein hemaglutinin dengan berat molekul 38,19 kDa memberikan titer tertinggi (⅟16). Hasil uji adhesi protein hemaglutinin yang disalutkan pada sel endotel menunjukkan bahwa semakin tinggi pengenceran maka adhesi bakteri menunjukkan peningkatan secara signifikan dengan konstanta regresi (r)= 0,98 dan p value= 0,00. Berdasarkan penelitian dapat disimpulkan bahwa protein hemaglutinin pili merupakan protein adhesin. Protein adhesin dengan berat molekul 38,19 kDa berperan pada perlekatan bakteri P. aeruginosa 9064 dengan kultur sel endotel (HUVECs). Kata kunci: adhesi, hemaglutinasi, pili, Pseudomonas aeruginosa

Effect of Cell Density and Benzyl Amino Purine on the Growth of Somatic Embryo of Citrus Mandarin Batu 55 (Citrus reticulata Blanco.) in Liquid Culture Nawafila Februyani; Wahyu Widoretno; Serafinah Indriyani
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Citrus mandarin Batu 55 (Citrus reticulata Blanco.) is one of Indonesian fruits commodities that have high economic value and consumers demand. The propagation of citrus mandarin by plant tissue culture generally was carried out on solid medium. The liquid culture system could increase cell multiplication therefore it became alternative method of plant propagation through somatic embryogenesis. The effect of initial cell density and Benzyl Amino Purin (BAP) concentration in liquid media were investigated. The initial cells density and right concentration of BAP given in media can increase cell proliferation of somatic embryo in liquid culture. Globular somatic embryo were cultured on Murashige and Tucker media with initial cell density 4, 6, 8 and 10 mgL-1 and BAP 0, 0.25, 0.5, and 0.75 mgL-1. Growth evaluation of somatic embryo were obtained by weighing fresh and dry weight every 2 weeks for 8 weeks for initial cell density treatment and 6 weeks of BAP treatment. The result of the research showed that cell density affect the growth of somatic embryo of citrus mandarin. Somatic embryo with low cell density showed slower growth compared than high cell density. Peak growth occured in 6th cultured with cell density 10 mgL-1. In addition to cell density, the growth of somatic embryo in liquid culture was affected by BAP. The growth of somatic embryo on the media containing BAP showed better results than without BAP. The highest BAP concentration on media showed fresh and dry weight of somatic embryo increased. In this research, growth of somatic embryo is not optimal yet because fresh and dry weights of somatic embryo still increase with high concentration 0.75 mgL-1 of BAP.Keywords: Benzyl Amino Purine, cell density, citrus mandarin, liquid medium, somatic embryo.

Screening of Rhizosphere Bacteria from Clove (Syzygium aromaticum) in Tidore Island as Plant Growth Promoting Rhizobacteria Ismat Ishak; Tri Ardyati; Luqman Qurata Aini
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Tidore Island in North Maluku Province is one of the clove (Syzygium aromaticum) producing regions. Clove plant fertility is maintained even though it is not given organic and inorganic fertilizers, it also keeps the fertility of the soil and the environment as a whole. Therefore, this study aims to explore bacteria in the rhizosphere of the clove trees planted in Tidore island as biofertilizer agents of Indol-3-Acetic Acid (IAA) production, phosphate solubilization, and ammonium production. Bacteria were isolated from the rhizosphere of clove trees. Screening of the isolates was done according to qualitative and quantitative methods. Data collection of the qualitative method were based on medium color changes for each variable while that the quantitative method (i.e. the concentrations of produced IAA, solubilized phosphate, and produced ammonium) were obtained based on spectrophotometry. The results obtained 110 bacterial isolates. Nineteen bacteria were potential as biofertilizer agents, of which isolate R11, R8P, and A1N showed the highest in IAA production (8.71 μgL-1), phosphate solubilization (8.37 μgL-1), and ammonium production (11.71 μgL-1), respectively. The three isolates respectively have similarities to the genus of Enterobacter, Burkholderia, and Stenotrophomonas. Keywords: Biofertilizer, Clove, Rhizobacteria, Rhizosphere.

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Home Page

OAI Link

Editorial Team

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

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Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA