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INDONESIA
Health Science Journal of Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 20877021     EISSN : 23383437     DOI : https://doi.org/10.22435/hsji
Core Subject : Health,
Dentistry Health Professions Nursing Public Health
Health Science Journal of Indonesia is a journal developed to disseminate and discuss the scientific literature and other research on the development of health. This journal is intended as a medium for communication among stake holders on health research such as researchers, educators, students, practitioners of Health Office, Department of Health, Public Health Service center, as well as the general public who have an interest in the matter. The journal is trying to meet the growing need to study health. Vision: Becoming a notable national journal in the field of health research and towards a reputable international journal. Mission: Providing scientific communication media in health research in order to advance science and technology in related fields. Organizes scholarly journal publishing in health research with an attempt to achieve a high impact factor in the development of science and technology.
Arjuna Subject : Kedokteran - Kesehatan Masyarakat, Kesehatan Lingkungan dan Pekerjaan
Articles 95 Documents
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Front Matter HSJi Volume 10 no.1 2019 hsji author
Health Science Journal of Indonesia Vol 10 No 1 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Front Matter HSJi Volume 10 no.1 2019
Back Matter HSJI Volume 10 no.1 2019 hsji author
Health Science Journal of Indonesia Vol 10 No 1 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Back Matter HSJI Volume 10 no.1 2019
Profile of malondialdehyde (MDA) and catalase specific activity in plasma of elderly woman Novi Silvia Hardiany; Sucitra Sucitra; Reni Paramita
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.2239

Abstract

Latar belakang: Malondialdehida (MDA) merupakan petanda stres oksidatif yang merupakan produk akhir dari reaksi berantai proksidasi lipid. Untuk mencegah stres oksidatif, tubuh mensintesis katalase, suatu enzim antioksidan endogen yang mengkatalisis hidrogen peroksida (H2O2) menjadi air dan oksigen. Sampai saat ini kadar MDA dan katalase pada populasi usia lanjut (usila) masih memberikan hasil yang bervariasi dan kadar tersebut pada kelompok usia yang berbeda dalam populasi usila belum pernah dilaporkan. Dengan demikian, penelitian ini bertujuan untuk menganalisis profil kadar MDA dan aktivitas spesifik katalase pada plasma populasi usila berdasarkan peningkatan usia. Metode: Penelitian ini menggunakan 60 subjek wanita usila sehat yang tinggal di Jakarta. Subjek dibagi dalam 2 kelompok berdasarkan kategori usia, kelompok yang lebih muda (60 – 70 tahun) dan kelompok yang lebih tua (> 70 tahun). Kadar MDA dan aktivitas spesifik katalase dianalisis pada plasma dengan spektrofotometer. Hasil: Kadar MDA pada kelompok yang lebih muda (60 – 70 tahun) sedikit lebih tinggi dibandingkan kelompok yang lebih tua (> 70 tahun) namun tidak bermakna secara statistik. Selain itu, akitivitas spesifik katalase pada kelompok yang lebih muda lebih rendah bermakna dibandingkan dengan kelompok yang lebih tua. Kesimpulan: Tidak ada perbedaan bermakna kadar MDA plasma pada populasi usila. Namun, aktivitas spesifik katalase meningkat bermakna seiring dengan pertambahan usia. (Health Science Journal of Indonesia 2019;10(2):132-6) Kata kunci: Malondialdehida, katalase, wanita usila Abstract Background: Malondialdehyde (MDA) is a marker of oxidative stress as an end product from the chain reaction of lipid peroxidation. In order to prevent oxidative stress, our body synthesizes catalase, an endogenous antioxidant enzyme that catalyzes hydrogen peroxide (H2O2) into water and oxygen. Until now, the level of MDA and catalase in aging population were still varied and those level at different age in elderly population has not been yet reported. Therefore, the purpose of this study was to analyse the profile of MDA level and catalase specific activity in plasma of elderly women based on increasing age. Methods: This research used 60 healthy elderly women as the subjects living in Jakarta. The subjects were divided into 2 groups based on age category, the younger group (60 – 70 years old) and the older group (>70 years old). MDA and specific activity of catalase were analyzed in plasma using spectrophotometer. Results: MDA level in the younger group (60-70 years old) was slightly higher than MDA levels in the older group (>70 years old) but it was not significant. Moreover, specific activity of catalase in the younger group was significantly lower than the older group. Conclusions: There was no difference in MDA level of elderly woman between younger and older group. However, catalase specific activity significantly increased with increasing age. (Health Science Journal of Indonesia 2019;10(2):132-6) Keywords: Malondialdehyde, Catalase, elderly woman
The effect of ethyl acetat fraction of Caesalpinia sappan L. wood on PC3 cancer cell line : cell viability and migration study Suyatmi Suyatmi; Endang Listyaningsih Suparyanti; Riza Novierta Pesik
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.2349

Abstract

Latar Belakang: Tingginya insidensi kanker di Indonesia, termasuk kanker prostat menimbulkan beban ekonomi kesehatan yang tinggi bagi Indonesia. Pengembangan terapi kanker berbasis sumber daya alam lokal dapat membantu meringankan beban negara. Penelitian ini bertujuan untuk mengetahui potensi aktivitas anti-kanker fraksi ethyl acetat Caesalpinia sappan L. terhadap sel line kanker PC3 yang merupakan model in vitro kanker prostat. Metode: Fraksi ethyl acetat kayu secang (Caesalpinia sappan L.) diperoleh melalui proses liquid chromatography. Efek Fraksi 9 dari Fraksi ethyl acetat kayu secang terhadap aktivitas anti-proliferasi dan migrasi sel diuji menggunakan desain uji in vitro. Hambatan proliferasi sel diukur dengan metode MTT assay, sedangkan aktivitas migrasi sel diukur dengan metode migration assay Hasil: Fraksi 9 dari Fraksi Ethyl acetat kayu secang memperlihatkan hambatan proliferasi sel line kanker PC3 dengan IC50:14.99μg/ml. Hasil migration assay menunjukkan pada dosis 10μg/ml Fraksi 9 menghambat migrasi sel line kanker PC3, sedangkan pada dosis 100μg/ml sel line kanker PC3 mati. Kesimpulan: Fraksi 9 dari fraksi Ethyl acetat kayu secang menunjukkan aktifitas anti-proliferasi dan anti-migrasi yang kuat terhadap pertumbuhan sel line kanker PC3 secara in vitro. Kata kunci : Ethyl Acetat fraction, Caesalpinia sappan, prostate cancer, PC3, migrasi sel Abstract Background: The high incidence of cancer, including prostate cancer, in Indonesia create a high burden on health economic cost. Development of cancer therapy based on local natural resources may help the country to alleviate the burden. This research aimed to find out the potency of selected compound of Ethyl Acetate fractions of Caesalpinia sappan as anti-cancer by using PC3 cancer cell line as an in vitro model of prostate cancer. Methods: Ethyl Acetate fraction of Caesalpinia sappan L.heartwood was prepared using a liquid chromatography method. The effect of Ethyl acetate fraction 9 on anti-proliferative and cell migration activities was assessed using MTT assay and migration assay. Results: Fraction-9 of Ethyl Acetate fraction of Caesalpinia sappan L. wood showed inhibition of PC3 cancer cell line proliferation. The IC50 of the fraction was 14.99μg/ml. The migration assay showed inhibition of cell migration on dose 10μg/ml compared to the 0 doses, while most of the cells cultured was dead when treated with 100μg/ml Fraction 9. Conclusion: Ethyl Acetate fraction 9 of Caesalpinia sappan L heartwood possibly has anti-cancer properties based on its anti-proliferative and anti-migration activities against PC3 cancer cell line. Keywords: Ethyl Acetate fraction, Caesalpinia sappan, prostate cancer, PC3, cell migration
The safety of kidd-incompatible blood transfusion in a restricted setting: a case report elida marpaung
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.2439

Abstract

Latar belakang: Protein Kidd merupakan transporter urea pada sel darah merah. Walaupun jarang, adanya antibodi terhadap antigen ini dapat menyebabkan reaksi transfusi dan hemolytic disease of the newborn. Keberadaan anti-Jka dan anti-Jkb cukup jarang ditemukan pada pemeriksaan identifikasi antibodi pasien. Studi ini melaporkan kasus pasien dengan keberadaan anti-Jka and anti-Jkb, yang mendapat darah dengan kadar aglutinasi terendah pada kondisi dimana darah kompatibel sulit didapat sementara tindakan transfusi sangat dibutuhkan segera. Penyajian Kasus: Wanita, 36 tahun, G4P3A0, datang dengan perdarahan vaginam sejak sebulan terakhir. Dari hasil pemeriksaan USG, didapatkan adanya mola hidatidosa. Pasien memerlukan terapi kuret segera setelah anemia terkoreksi (Hb 8.3 g/dL). Pada uji kecocokan pre-transfusi dengan prosedur skrining antibodi yang dilanjutkan dengan identifikasi antibodi, ditemukan anti-Jka dan anti-Jkb. Dari setidaknya 50 darah donor yang dilakukan uji kecocokan, tidak ditemukan darah yang kompatibel, sehingga pasien diputuskan untuk mendapat transfusi menggunakan darah inkompatibel dengan derajat aglutinasi terendah (level 2) dari 5 level, disertai dengan pemantauan ketat terhadap potensi terjadinya reaksi transfusi. Demam dan pruritus dilaporkan dalam 24 jam setelah transfusi, dan membaik setelah pemberian injeksi difenhidramin, deksametason, dan parasetamol. Kesimpulan: Transfusi dengan darah yang inkompatibel merupakan pilihan terakhir bila tidak ditemukan darah donor yang kompatibel. Reaksi transfusi merupakan efek yang sulit dihindari, tetapi dapat dilakukan pemantuan ketat. Pemilihan darah dengan level aglutinansi terendah adalah keputusan terbaik, mengingat tindakan medis diperlukan segera untuk menyelamatkan nyawa. Pada kasus ini, pasien mendapat tatalaksana optimal dari aspek tindakan operasi dan respon transfusi, yang ditunjukkan melalui kenaikan nilai Hb yang bermakna. Sementara itu, efek samping reaksi transfusi yang muncul hanya ringan dan dapat ditanggulangi dengan pemberian obatobatan. (Health Science Journal of Indonesia 2019;10(2):137-9) Kata kunci: reaksi transfusi, inkompatibilitas, kelompok darah Kidd Abstract Background: Kidd protein is red blood cell’s (RBC) major urea transporter. Albeit rare, the presence of antibodies against Kidd antigen may cause significant hemolytic transfusion reaction and hemolytic disease of the newborn. Yet, anti-Jka and anti-Jkb are rare to be discovered during antibody identification. This paper reported “bestmatched” transfusion practice in a patient with anti-Jka and anti-Jkb, where compatible PRC cannot be found, but transfusion is urgently needed. Case Presentation: A 36 years old, G4P3A0 female, came with continuous vaginal bleeding for the past one month before admission. USG revealed hydatidiform mole. She needed immediate curettage following correction of her anemia (Hb 8.3g/dL). After antibody screening procedure followed by antibody identification, we found a positive anti-Jka and anti-Jkb in her blood sample. At least 50 blood donors were tested for compatibility and none was a match. She was then transfused with the lowest agglutination blood available (level 2 of 5 levels), with a closed monitoring to anticipate the possibility of transfusion reaction development. Fever and pruritus transpired within 24 hours post transfusion and it resolved following diphenhydramine, dexamethasone, and paracetamol injection. Conclusion: Incompatible blood transfusion is the last option when compatible blood cannot be found. The development of transfusion reaction is inevitable, but it can be anticipated by closed monitoring. In restricted setting, blood transfusion with the lowest level of agglutination is acceptable when transfusion is imperative. In this case, the patient got optimal treatment in term of the medical surgery and transfusion response, which was shown by the significant increase of Hb level. Meanwhile, the adverse transfusion reaction was only mild, and could be treated with medicine. (Health Science Journal of Indonesia 2019;10(2):137-9) Keywords: Transfusion reaction, incompatibility, Kidd blood group
Increasing serum miR-124-3p expression is associated with the high survival rate of a rectal cancer patient after neoadjuvant chemoradiotherapy Sri Nuryani Wahyuningrum; Christina Hari Nawangsih Priharsanti; Sofia Mubarika Haryana; Ahmad Ghozali
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v10i2.2440

Abstract

Latar Belakang: Kanker kolorektal menempati urutan ketiga penyebab kanker di dunia, dengan prevalensi kanker rektum sebanyak 30% dari total kasus. Saat ini belum ada biomarker yang efektif untuk memprediksi respon pasien terhadap terapi yang diberikan. Beberapa penelitian menggunakan potensi miRNA sebagai biomarker untuk melihat respon terapi. Salah satunya yaitu MiR-124-3p berperan sebagai tumor supresor yang mengalami penurunan ekspresi pada berbagi jenis kanker. Tujuan dari penelitian ini adalah untuk meneliti ekspresi miR-124-3p dari pasien kanker rektum yang menerima nCRT, dan menganalisis hubungannya dengan kelangsungan hidup pasien dan parameter klinis lainnya. Metode: Penelitian ini melibatkan 15 orang pasien yang didiagnosis menderita kanker rektum lokal dan menjalani kemoradioterapi neoajuvan (radioterapi 45-50 Gy dengan fraksi 1,8-2 Gy selama 1-3 bulan, dan kemoterapi 5-fluororacil secara oral). Sampel penelitian berupa darah intravena sebanyak 5 ml diambil saat sebelum dan sesudah kemoradioterapi. Selanjutnya ekspresi miR-124-3p dianalisis menggunakan qRT-PCR dan dikalkulasi menggunakan metode Livak. Hasil: Terdapat hubungan signifikan antara peningkatan ekspresi miR-124-3p dengan sintasan hidup pasien (P=0,003; OR =30, 95% CI = 1,41 – 638,15), serta adanya peningkatan ekspresi miR-124-3p yang signifikan (P<0,041, fold change sebelum=1,14 ± 1,25; sesudah=2,4 ± 1,84) setelah dilakukan kemoradioterapi. Kesimpulan: Hasil ini mengindikasikan bahwa miR-124-3p berpotensi menjadi biomarker untuk memprediksi sintasan hidup pasien kanker rektum yang menerima kemoradioterapi. (Health Science Journal of Indonesia 2019;10(2):90-5) Kata kunci: kanker rektum, kemoradioterapi, miR-124-3p, sintasan hidup Abstract Background: Colorectal cancer is the world’s third most prevalent cancer, which 30% of cases are rectal cancer. Today, the effective diagnostic marker to accurately predict clinical outcome patients response to therapy did not found yet. Several research studies have indicated that miRNA potential as a prognostic biomarker. MiR 124-3p plays as tumor suppressor that significantly down-regulated in some cancer and could radiosensitize human colorectal cancer cells. The aim of the study is to investigate the expression of miR-124-3p from rectal cancer patient who receive nCRT, and analyze its association with patient survival and others clinical parameters. Methods: This research involved 15 patients with histologically confirmed locally advanced rectal cancer (LARC) and received neoadjuvant chemotherapy/nCRT (radiotherapy 45-50 Gy with 1,8-2 Gy fractions over 1 to 3 months and chemotherapy 5-fluorouracil was administered orally). Patient blood (5 ml) were collected from peripheral venous before and after neoadjuvant chemoradiotherapy. miR-124-3p expression was performed using qRT-PCR and calculate using Livak method. Results: In this study, we found that increasing of miR-124 was significantly associate with high survival of rectal cancer patient (P = 0,003; OR =30, 95% CI = 1,41 – 638,15). Average of miR-124-3p expression increase significantly after nCRT (P<0,041, fold change before=1,14 ± 1,25; after=2,4 ± 1,84). Conclusion: Our finding suggests that miR-124-3p expression in blood serum was potential as biomarkers to predict rectal cancer patient survival after neoajduvant chemoradiotherapy. (Health Science Journal of Indonesia 2019;10(2):90-5) Keywords : rectal cancer, chemoradiotherapy, miR-124-3p, survival
The effect of ethanolic leaves extract of soursop (Annona muricata L.) on human colorectal cancer cell line: cell viability and in silico study to cyclin D1 protein Murdani Abdullah; Debby Desmarini; Sofy Meilaini; Puji Sari; Luluk Yunaini; Fadilah Fadilah
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.2441

Abstract

Latar Belakang: Kanker kolorektal merupakan transformasi patologis dari epitel kolon dan rektum normal menjadi massa jaringan abnormal, perubahan ini terjadi karena ekspresi berlebih dari protein cyclin D1 yang menginduksi proliferasi sel kolorektal secara berlebihan. Pengobatan dan pencegahan kanker kolorektal dapat dilakukan secara alami dengan mengonsumsi ekstrak daun Annona muricata L. (sirsak). Sirsak dikenal karena banyak komponen fitokimia yang berfungsi sebagai anti kanker. Metode: Penelitian ini menggunakan sel kanker kolorektal HT-29 yang diberi ekstrak etanol daun sirsak dan 5 Fluorourasil (5-FU). Tujuannya untuk menemukan konsentrasi sitotoksisitas yang dapat menghambat 50% populasi sel HT-29 (CC50) dan konsentrasi yang didapat sebelumnya akan diuji dengan metode uji MTT. Analisis docking molekuler dilakukan antara molekul-molekul dari ekstrak etanol daun sirsak terhadap protein Cyclin D1 menggunakan perangkat lunak molecular operating environment (MOE) 2013.08. Hasil: CC50 ekstrak etanol daun sirsak adalah 278 μg / mL dan 5-FU adalah 88 μg / mL. Persentase terendah sel HT-29 yang layak adalah 2 x CC50 setelah perlakuan ekstrak etanol daun sirsak (40,4 ± 1,3%) dibandingkan dengan 5-FU (52,8 ± 4,3%), kontrol pelarut ( 97,2 ± 1,4%), dan kontrol sel (100%). Analisis docking molekuler untuk protein cyclin D1 diperoleh asam N-hexadecanoic dan molekul phytol sebagai kandidat yang baik untuk menghambat protein cyclin D1. Kesimpulan: Ekstrak etanol daun sirsak dapat menurunkan viabilitas sel kultur kanker kolon HT-29 dan berdasarkan analisis molekular docking dilihat dari energi bebas gibbs (ΔG) dan afinitas tertinggi (pKi) diperoleh N-hexadecanoic dan molekul phytol sebagai penghambat protein cyclin D1. (Health Science Journal of Indonesia 2019;10(2):96-102) Kata Kunci: Kanker kolorektal HT-29, ekstrak etanol daun sirsak, viabilitas sel, molecular docking, cyclin D1 Abstract Introduction: Colorectal cancer is a pathological transformation of normal colon and rectum epithelial that becomes an abnormal tissue mass, due to the overexpression of cyclin D1 protein that inducing excessive proliferation of colorectal cell. The treatment and prevention of colorectal cancer could be done naturally by consuming leaves extract of Annona muricata L. (soursop). Soursop is known for many phytochemical components that serve as an anti-cancer. Methods: This study was used HT-29 colorectal cancer cell that treated with ethanolic leaves extract of soursop and 5-Fluorourasil (5-FU) to find the cytotoxicity concentration that can inhibit 50% of HT-29 cell population (CC50) and the next concentrations of them were treated for next treatment with MTT assay. Molecular docking analysis of the compounds of ethanolic leaves extract of soursop to cyclin D1 protein used molecular operating environment (MOE) 2013.08 software. Results: CC50 of ethanolic leaves extracts of soursop was 278 μg/mL dan 5-FU was 88 μg/mL. The lowest percentage of viable HT-29 cell was 2 x CC50 after ethanolic leaves extract of soursop treatment (40,4±1,3%) was compared to 5-FU (52,8±4,3%), solvent control (97,2±1,4%), and cells control (100%). Analysis of molecular docking to cyclin D1 protein was obtained N-hexadecanoic acid and phytol molecules as good candidates to inhibit cyclin D1 protein. Conclusions: The ethanolic leaves extract of soursop could be a good alternative treatment for colorectal cancer and its compounds had ability to inhibit cyclin D1 protein (the highest gibbs free energy (ΔG) and affinity (pKi)). (Health Science Journal of Indonesia 2019;10(2):96-102) Keywords: Colorectal cancer, ethanolic leaves extract of soursop, cell viability, molecular docking, cyclin D1
Cloning and expression of Human Papilloma virus type 16 L1 capsid protein in bacteria Silvia Tri Widyaningtyas; Sofy Meilany; Budiman Bela
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.2442

Abstract

Latar belakang: Secara alamiah protein kapsid L1 Human Papillomavirus (HPV) tipe 16 dapat mengalami auto assembly untuk membentuk Viral like particle (VLP). Terkait dengan penelitian vaksin HPV, VLP dapat digunakan untuk berbagai keperluan seperti vaksin, pseudovirion atau SpyTag-Spycatcher. Penelitian ini ditujukan untuk mendapatkan plasmid rekombinan yang digunakan untuk produksi protein L1 HPV 16. Metode: Gen penyandi protein L1 HPV 16 diklona ke dalam vector pQE80L, suatu plasmid yang mengandung sistem ekspresi untuk prokariota. DNA penyandi HPV 16 L1 disisipkan pada situs restriksi BamHI dan Hind III plasmid pQE80L. Plasmid rekombinan yang mengandung gen L1 HPV 16dikonfirmasi menggunakan PCR dan analisis enzim restriksi. Lebih lanjut untuk memastikan bahwa gen rekombinan L1 HPV 16 dapat diekspresikan dalam prokariota, plasmid rekombinan ditransformasikan ke bakteri Escherichia coli BL21 (DE3). Bakteri diinduksi dengan Isopropyl β-D-1-thiogalactopyranoside (IPTG) dengan berbagai konsentrasi dan berbagai waktu inkubasi. Hasil: protein rekombinan L1, berat 56 kDa, telah berhasil diekspresikan dalam sistem prokariota. Protein rekombinan L1 dapat dimurnikan menggunakan TalonR dalam kondisi denaturasi. Kesimpulan: gen L1 HPV 16 telah dikloning ke dalam pQE80L dan berhasil diekspresikan dalam sistem prokariota. (Health Science Journal of Indonesia 2019;10(2):82-9) Kata kunci: L1, HPV 16, cervical cancer Abstract Background: Naturally Human Papillomavirus (HPV) type 16 L1 capsid protein can auto assemble to form Viral like particles (VLP). Concerning to vaccine development for HPV, VLP can be used for a variety of needs such as a vaccine, pseudovirion or SpyTag-Spycatcher. In this study, to obtain a vector expression that can be used in the production of HPV L1 protein, we cloned gene coding HPV 16 L1 protein into pQE80L a plasmid contains an expression system for prokaryote. Methods: The DNA coding HPV 16 L1 was inserted at BamHI and Hind III restriction sites of pQE80L plasmid. The recombinant plasmid containing the HPV L1 gene was confirmed using PCR colony and enzyme restriction. Further to ensure the recombinant HPV 16 L1 gene could be expressed in a prokaryote, the recombinant plasmid was transformed into bacteria Escherichia coli BL21 (DE3). The bacteria were induced with IPTG with various concentrations and various incubation time. Result: L1 recombinant protein, 56 kDa in weight, has successfully been expressed in prokaryote system. L1 recombinant protein can be purified using TalonR under denaturing conditions. Conclusion: L1 HPV 16 gene has been cloned into pQE80L and successfully expressed in prokaryote system. (Health Science Journal of Indonesia 2019;10(2):82-9) Keywords: L1, HPV 16, cervical cancer
Correlation between dietary fat consumption with body mass index and body composition (a preliminary study in community based) Amelya Augusthina Ayusari; Budiyanti Wiboworini; Kusmadewi Eka Damayanti; Dwi Rahayu; Widardo Widardo; Yulia Lanti
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.2443

Abstract

Pendahuluan: Sejumlah penelitian menunjukkan hubungan antara konsumsi lemak dengan indeks massa tubuh dan komposisi tubuh. Penelitian ini bertujuan untuk mengetahui hubungan antara konsumsi harian lemak total, asam lemak tak jenuh ganda (PUFA) dan kolesterol total dengan beberapa paramater gizi. Metode: Penelitian ini merupakan studi cross-sectional, dengan 102 subjek. Pemeriksaan yang dilakukan adalah tinggi badan, berat badan, indeks massa tubuh dan pengukuran komposisi tubuh menggunakan timbangan komposisi tubuh Omron® HBF-212. Analisis konsumsi lemak total, asam lemak tak jenuh ganda dan kolesterol total menggunakan Software nutrisurvey 2007. Uji korelasi yang digunakan adalah Spearman Rho dengan menggunakan SpSS 21. Hasil: Konsumsi lemak total tidak berhubungan dengan indeks massa tubuh, massa lemak total, dan massa lemak visceral. Konsumsi PUFA berhubungan secara negatif dengan indeks massa tubuh (p <0,014, -0,24) dan massa lemak total (p <0,001, -0,326), sedangkan konsumsi total kolesterol total berhubugan secara negatif dengan indeks massa tubuh (p <0,019, -0,23), dan massa lemak total (p <0,001, -0,337). Kesimpulan: Ada hubungan antara konsumsi lemak dengan indeks massa tubuh dan komposisi tubuh. (Health Science Journal of Indonesia 2019;10(2):128-31) Kata kunci: konsumsi lemak; indeks massa tubuh; komposisi tubuh Abstract Introduction: Studies showed some relation between fat consumption with body mass index and body composition. We conducted a study to investigate relationships between daily consumption of total fat, polyunsaturated fatty acid (PUFA) and total cholesterol with some nutritional parameters. Methods: This was cross-sectional study, with 102 subjects. The study was examined height, body weight, body mass index and body composition measurements using the Omron® HBF-212 body composition monitor. There was nutrisurvey 2007 to measure total fat, polyunsaturated fatty acid and total cholesterol consumption. We analyzed the correlation by using SpSS 21 (Spearman Rho) Results: Total fat consumption was not related to body mass index, total fat mass, and visceral fat mass. PUFA consumption was negatively associated with body mass index (p <0.014, -0.24) and total fat mass (p <0.001, -0.326), while consumption of total cholesterol was negatively associated with body mass index (p <0.019, -0.23), and total fat mass (p <0.001, -0.337) Conclusion: There was a relation between fat consumption with body mass index and body composition.(Health Science Journal of Indonesia 2019;10(2):128-31). Keywords: fat consumption; body mass index; body composition
Perceptions of pregnant woman on monetary and time sacrifice for satisfaction based on health workers roles in antenatal services to reduce the risk of maternal death at Gowa district Serawati Dewi; Yuni Romalita; Yusriani Yusriani; Muhammad Khidri Alwi
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v10i2.2444

Abstract

Latar belakang: Angka Kematian Ibu (AKI) merupakan salah satu indikator pembangunan kesehatan di Indonesia. Upaya percepatan penurunan AKI dapat dilakukan dengan menjamin agar setiap ibu mampu mengakses pelayanan kesehatan ibu hamil yang berkualitas. Apabila antenatal care dimanfaatkan dengan baik maka kesehatan ibu dapat terpantau secara berkesinambungan dari masa kehamilan sampai dengan persalinan. Metode: Desain penelitian adalah kuantitatif dengan rancangan cross sectional study. Populasi pada penelitian ini adalah semua ibu yang memiliki bayi usia 0-11 bulan di Kabupaten Gowa sebanyak 122 orang. Sampel sebanyak 93 orang diambil dengan menggunakan teknik accidental sampling, besar sampel ditentukan dengan menggunakan rumus Slovin Hasil: Persepsi ibu hamil terhadap peran petugas kesehatan dalam pelayanan antenatal berdasarkan pengorbanan moneter tidak menunjukkan korelasi. Ibu hamil yang memiliki persepsi pengorbanan moneter kecil dan mengatakan peran petugas kesehatan kurang dalam pelayanan antenatal sebanyak 90,0%. Ibu hamil yang memiliki persepsi pengorbanan moneter sangat kecil dan mengatakan peran petugas kesehatan kurang sebanyak 83.1%. Sedangkan persepsi pengorbanan waktu menunjukkan adanya korelasi dengan peran petugas kesehatan dalam pelayanan antenatal. Ibu hamil yang memiliki persepsi pengorbanan waktu besar dan mengatakan peran petugas kesehatan kurang dalam pelayanan antenatal sebanyak 100%. Ibu hamil yang memiliki persepsi pengorbanan moneter besar dan mengatakan peran petugas kesehatan kurang dalam pelayanan antenatal sebanyak 90.2%. Kesimpulan: Persepsi ibu hamil terhadap peran petugas kesehatan dalam pelayanan antenatal berdasarkan pengorbanan moneter tidak menunjukkan korelasi sedangkan berdasarkan pengorbanan waktu menunjukkan ada korelasi. Perlu meningkatkan kecepatan proses pelayanan pemeriksaan kehamilan pada ibu hamil oleh tenaga kesehatan. Kata kunci: Pelayanan antenatal, Ibu Hamil, Pengorbanan, Waktu, Moneter Abstract Background: Maternal Mortality Rate (MMR) is one indicator of health development in Indonesia. Efforts to accelerate the reduction of MMR can be done by ensuring that every mother is able to access quality maternal health services. If antenatal care is utilized properly, maternal health can be monitored continuously from pregnancy to delivery. Methods: The study design was quantitative with a cross sectional study design. The population in this study were all pregnant women who had a gestational age of 7 to 9 months in Gowa Regency as many as 122 people. A sample of 93 people was taken using accidental sampling technique, the sample size was determined using the Slovin formula. Results: Perception of pregnant women towards the role of health workers in antenatal care based on monetary sacrifice did not show a correlation. Pregnant women who have a perception of small monetary sacrifice and say the role of health workers is lacking in antenatal care are 90.0%. Pregnant women who have a perception of monetary sacrifice are very small and say the role of health workers is less as much as 83.1%. While the perception of sacrifice of time shows a correlation with the role of health workers in antenatal care. Pregnant women who have the perception of sacrifice of big time and say the role of health workers lacking in antenatal care as much as 100%. Pregnant women who have a perception of great monetary sacrifice and say the role of health workers is lacking in antenatal care are 90.2%. Conclusion: Perception of pregnant women towards the role of health workers in antenatal care based on monetary sacrifice does not show correlation while based on time sacrifice shows there is correlation. Need to increase the speed of the process of pregnancy examination services for pregnant women by health workers. Keywords: Antenatal care, Pregnant Women, Sacrifice, Time, Monetary

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