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INDONESIA
Health Science Journal of Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 20877021     EISSN : 23383437     DOI : https://doi.org/10.22435/hsji
Core Subject : Health,
Dentistry Health Professions Nursing Public Health
Health Science Journal of Indonesia is a journal developed to disseminate and discuss the scientific literature and other research on the development of health. This journal is intended as a medium for communication among stake holders on health research such as researchers, educators, students, practitioners of Health Office, Department of Health, Public Health Service center, as well as the general public who have an interest in the matter. The journal is trying to meet the growing need to study health. Vision: Becoming a notable national journal in the field of health research and towards a reputable international journal. Mission: Providing scientific communication media in health research in order to advance science and technology in related fields. Organizes scholarly journal publishing in health research with an attempt to achieve a high impact factor in the development of science and technology.
Arjuna Subject : Kedokteran - Kesehatan Masyarakat, Kesehatan Lingkungan dan Pekerjaan
Articles 95 Documents
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Quality of life among ovarian cancer survivors in Haji Adam Malik General Hospital Medan, Indonesia Kristivani Br Ginting; Muhammad Rizki Yaznil; M. Oky Prabudi; Lili Rahmawati
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3080

Abstract

Latar belakang: Kanker ovarium memiliki angka mortalitas yang cukup tinggi dikarenakan gejalanya yang tidak spesifik, sering ditemukan pada stadium lanjut, dan belum adanya metode deteksi dini yang sudah terbukti. Untuk menilai keberhasilan terapi penyintas kanker ovarium, tidak hanya dinilai dari aspek klinis tetapi juga dinilai dari kualitas hidup penyintas kanker ovarium yang penilaiannya berdasarkan skala fungsional dan skala gejala dalam kuesioner EORTC QLQ C30 dan EORTC QLQ OV28. Metode: Penelitian ini menggunakan desain penelitian cross sectional, menggunakan data primer dari hasil wawancara dengan kuesioner EORTC QLQ C30 dan EORTC QLQ OV28 serta data sekunder yang berasal dari rekam medik di RSUP Haji Adam Malik Medan tahun 2017 - 2018. Sampel penelitian dipilih dengan metode total sampling dari seluruh data rekam medik yang memenuhi kriteria penelitian. Hasil: Hasil penelitian ini didapatkan kualitas hidup global penyintas kanker ovarium 89.36% adalah baik, dan 10.64% adalah sedang serta tidak ada yang memiliki kualitas hidup buruk. Namun, didapatkan adanya gangguan pada skala fungsional berupa: fungsi emosional, fungsi kognitif, fungsi seksual, dan sikap terhadap penyakit, serta adanya permasalahan pada skala gejala berupa: kelelahan, nyeri, neuropati perifer, dan gejala menopause. Didapatkan juga tidak ada hubungan karakteristik usia, jenis histopatologis, stadium, lama terapi dengan kualitas hidup penyintas kanker ovarium, namun terdapat hubungan antara jenis terapi dengan kualitas hidup penyintas kanker ovarium. Kesimpulan: Kualitas hidup penyintas kanker ovarium secara global adalah baik. Kata Kunci: Kualitas Hidup, Penyintas Kanker Ovarium, EORTC QLQ C-30, EORTC QLQ OV-28 Abstract Background: Ovarian cancer has a high mortality rate due to nonspecific symptoms, often found at an advanced stage, and also the absence of proven early detection methods. To assess the success of ovarian cancer survivors therapy, it is not only assessed from the clinical aspect but also from the quality of life of ovarian cancer survivors which is based on the functional and symptom scale in the EORTC QLQ C30 and EORTC QLQ OV28 questionnaires. Methods: This study used a cross-sectional study design, using primary data from interviews with the survivors based on the questionnaire EORTC QLQ C30 and EORTC QLQ OV28 as well as secondary data derived from medical records at Haji Adam Malik General Hospital Medan in 2017 - 2018. The research sample was used with a total sampling method from all medical record data that fulfill the research criteria. Result: The quality of life of ovarian cancer survivors is generally good (89.36%), meanwhile the rest is moderate (10.64%) without the poor quality of life. However, there are disorders on the functional scale in the form of emotional function, cognitive function, sexual function, and attitude toward disease. Likewise on the scale of symptoms, there are problems including: fatigue, pain, peripheral neuropathy, and menopausal symptoms. Conclusion: The quality of life of ovarian cancer survivors globally is good. Keywords: Quality of Life, Ovarian Cancer Survivors, EORTC QLQ C-30, EORTC QLQ OV-28
Comparison of BCYE and BMPA media on recovery rate of Legionella pneumophila Lucky Moehario; Enty Tjoa; Mutiara J. Taslim; Yohanna Angelina
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i1.3127

Abstract

Latar belakang: Legionella pneumophila (L. pneumophila) telah banyak diketahui sebagai penyebab legionellosis; habitat nya di berbagai sumber air, lingkungan lembab dan hangat. Metode kultur menggunakan media spesifik masih menjadi baku emas untuk identifikasi L. pneumophila. Penelitian ini bertujuan membandingkan recovery rate L. pneumophila ATCC®33823 pada medium spesifik BCYE, dan medium selektif BMPA. Metode: Dilakukan dilusi serial pada suspensi 0,5 McFarland L. pneumophila ATCC®33823; 100 ul dari tiap tingkat dilusi diinokulasi pada medium Buffered Charcoal Yeast Extract (BCYE) dan medium BMPA (medium BCYE yang ditambahkan suplemen BMPA-α), secara duplo. Konsentrasi suspensi dihitung menggunakan metode Angka Lempeng Total (ALT) dari Standar Nasional Indonesia No. 01-2332.3-2006. Persentase recovery rate dihitung dan dianalisis secara statistik menggunakan SPSS versi 23,0. Hasil: Jumlah koloni L. pneumophila yang tumbuh pada medium BMPA jauh lebih tinggi daripada yang tumbuh di medium BCYE; konsentrasi tertinggi yang diperoleh adalah pada medium BMPA sebesar 1,45 x 107 CFU/ml. Persentase recovery rate pada medium BMPA adalah 96,67%, dan 60,67% pada medium BCYE. Kesimpulan: Recovery rate medium BMPA untuk pertumbuhan koloni L. pneumophila ATCC®33823 jauh lebih tinggi daripada media BCYE, karena itu medium BMPA dapat direkomendasikan untuk kultivasi L. pneumophila, khususnya pada program surveilans berbagai sumber air dengan biaya lebih terjangkau. Kata kunci: Legionella pneumophila, medium spesifik, BCYE , BMPA, recovery rate Abstract Background: Legionella pneumophila (L. pneumophila) has been known as the etiology of legionellosis; they live in aquatic environment, warm and moist. Culture method using specific medium remains as the gold standard in the identification of L. pneumophila. This study aimed to compare the recovery rate of L. pneumophila ATCC® 33823 on the specific medium BCYE for the cultivation of Legionella, and BMPA, the selective medium. Methods: Suspension of L. pneumophila ATCC® 33823 of 0.5 McFarland was diluted to 10 fold serial dilution; 100 ul of each dilution was inoculated on Buffered Charcoal Yeast Extract (BCYE) medium, and BMPA (BCYE supplemented with BMPA-α) in duplicate manner. The concentration was calculated using Total Plate Count standard as of Indonesian Nasional Standard number 01-2332.3-2006. The percentage of recovery rate was calculated, and the statistical analysis was performed using SPSS version 23.0. Results: Numbers of colonies of L. pneumophila grew on BMPA was much higher than on BCYE medium; the highest concentration was yielded on BMPA medium i.e. 1.45x107 CFU/ml. The recovery rates were 96.67% and 60.67% on BMPA medium and BCYE subsequently. Conclusion: The recovery rate of the BMPA medium on the colony growth of L. pneumophila ATCC®33823 was markedly higher than the BCYE, therefore BMPA medium can be suggested to be used in the cultivation of L. pneumophila especially in the routine surveillance program for water sources with less cost. Keywords: Legionella pneumophila, specific medium, BCYE , BMPA, recovery rate
AFB smear positive 1+: a dominant factor in Pulmonary TB household transmission Alinea Dwi Elisanti; Diany Yoke Safira; Efri Tri Ardianto
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i1.3129

Abstract

Latar belakang: TBC menjadi masalah kesehatan dunia, termasuk Indonesia karena kasus baru TB paru terus meningkat. Penelitian ini mengkaji faktor dominant yang mempengaruhi penularan kontak serumah pasien TB paru. Metode: Studi observasional dengan desain analitik cross sectional ini menggunakan populasi seluruh penderita dan anggota keluarga pasien TB paru di Puskesmas Kedundung tahun 2015 2016. Sampel sejumlah 52 orang dihitung menggunakan rumus besar sampel infinith dan diambil secara konsekutif. Variabel penelitian meliputi perilaku pencegahan, Gradasi BTA dan penularan kontak serumah. Pengumpulan data menggunakan lembar wawancara, lembar pengumpul data, sputum pot steril. Data dianalisis menggunakan uji Spearman Rank dan uji regresi logistik binary dengan tingkat signifikansi 0,05. Hasil: Perilaku pencegahan (p=0.093), BTA positive 2+ (p=0.377) tidak berpengaruh terhadap penularan kontak serumah pasien TB paru, sedangkan BTA positive 1+ mempengaruhi penularan kontak serumah pasien TB paru (p= 0,007). Nilai Exp (B) BTA positif (1+) menunjukkan 12,144 artinya pasien BTA positif (1+) memiliki risiko 12,144 kali lebih tinggi menularkan ke kontak serumah dibandingkan dengan BTA positif (3+). Sedangkan pasien BTA positif (2+) memiliki risiko 3,328 kali menularkan ke kontak serumah dibandingkan dengan BTA positif (3+). Kesimpulan: Pasien TB paru dengan pemeriksaan BTA positif (1+) menjadi faktor dominan yang mempengaruhi penularan kontak serumah pasien TB paru. Upaya komunikasi dan edukasi personal higiene, tingkat kepatuhan pengobatan pasien TB paru perlu ditingkatkan untuk menekan kejadian baru TB paru. Kata kunci: Gradasi BTA, TB Paru, Perilaku Pencegahan, Penularan Kontak Rumah Tangga Abstract Background: Tuberculosis has become a global health problem, included in Indonesia, new cases of pulmonary TB increase continuously. This study examined the dominant factors that influenced the transmission of household contacts in pulmonary TB patients. Methods: This observational study was a cross-sectional analytic design which used a population of all patients and family members of pulmonary TB patients at the Kedundung Health Center in 2015 until 2016. A sample of 52 people was counted using the infinith sample size formula and taken consecutively. Research variables include prevention behavior, AFB smear gradation, and household contact transmission. Collecting data used interview sheets, data collection sheets, sterile sputum pots. Data was analyzed by chi-square test and binary logistic regression test with a significance level of 0.05. Results: Prevention behavior (p=0.093), AFB smear-positive 2+ (p=0.377) did not affect on household contact transmission in pulmonary TB patients, whereas AFB smear-positive 1+ affected household contacts transmission of pulmonary TB patients (p=0.007). The value of Exp (B), AFB smear-positive (1+) have a risk of 12.144 times transmitting to household contact when compared to AFB smear-positive (3+). Whereas patients with AFB smear-positive (2+) have a risk of 3,328 times transmitting to household contact when compared with AFB smear-positive 3+. Conclusion: Pulmonary TB patients with AFB smear-positive (1+) was the dominant factor affecting household contact transmission. Communication and personal hygiene education efforts, the level of adherence in the treatment of pulmonary TB patients needs to be increased to suppress the new incidence of pulmonary TB. Keywords: AcidFast Bacilli (AFB) gradation, pulmonary TB, Prevention Behavior, Transmission of Household Contacts
Dietary intake changes in adolescent girl after iron deficiency anemia diagnosis Dominikus Raditya Atmaka; Windi Indah Fajar Ningsih; Risnukathulistiwi Maghribi
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i1.3143

Abstract

Latar Belakang: Defisiensi zat besi dapat terjadi karena rendahnya konsumsi makanan yang mengandung tinggi zat besi dan tingginya konsumsi makanan yang dapat menghambat absorpsi zat besi. Meningkatkan asupan zat gizi adalah salah satu cara paling efektif untuk memutus rantai permasalahan anemia defisiensi zat besi. Tujuan penelitian ini untuk mempelajari perubahan asupan gizi dan kesadaran akan kesehatan pada remaja perempuan sebelum dan setelah diagnosis anemia defisiensi besi. Metode: Penelitian menggunakan desain kohort dengan subyek sebanyak 62 orang dari 2 Sekolah Menengah Pertama di Wates setelah dilakukan tes hemoglobin (metode cyanmethemoglobin) dan baru didiagnosis anemia defisiensi besi. Asupan energi, zat gizi makro (protein, lemak, karbohidrat), zat gizi mikro (zat besi, vitamin C, tembaga, zink, vitamin B12), faktor penghambat serapan (tanin, oksalat, fitat, serat), juga asupan, buah, sayur, kopi, dan teh diperoleh dengan semi quantitative food frequency questionnaire (SQFFQ), 3 bulan sebelum dan 3 bulan setelah diagnosis anemia defisiensi besi. Data dihitung dengan Nutrisurvey® and STATA 12® menggunakan paired T-test. Hasil: Tidak ada perubahan signifikan (p>0,05) pada asupan energi, protein, lemak, zink, vitamin B12, tembaga, serat, tanin, dan oksalat. Terdapat peningkatan signifikan asupan karbohidrat (p=0,0161), zat besi(p=0,0057), fitat (p=0,000), dan vitamin C (p=0,0017). Tidak ada perubahan signifikan rata-rata asupan buah, sayur, dan teh (p>0,05), tetapi konsumsi kopi lebih tinggi (p=0,0018). Kesimpulan: Diagnosis anemia defisiensi besi mengarahkan pada perubahan asupan zat gizi. Subyek menjadi lebih sadar pada asupan zat gizi setelah diagnosis anemia defisiensi besi. Dibutuhkan usaha lebih untuk merubah asupan buah dan sayur yang rendah dan konsumsi teh dan kopi yang tinggi. Kata Kunci: perubahan asupan gizi, kebiasaan makan, remaja perempuan, anemia defisiensi zat besi Abstract Background: Iron deficiency may happen because of low consumption of foods rich in bioavailable iron and high consumption of foods rich in inhibitors of iron absorption. Improving dietary intake is the most effective way to break the chain of iron deficiency anemia problems. This study aimed to study the changes in dietary intake and health awareness among adolescent girl before and after iron deficiency anemia diagnosis. Method: Cohort study with 62 subjects from 2 junior high school in Wates after hemoglobin test (cyanmethemoglobin method) and were newly iron deficiency anemia diagnosed. Intake of energy, macronutrient (protein, fat, carbohydrate), micronutrient (iron, vitamin C, Copper, Zinc, vitamin B12), inhibitor factors (tannin, oxalate, phytate, fiber), also intake of fruit, vegetable, coffee, and tea, collected using semi quantitative food frequency questionnaire (SQFFQ), 3 months before and 3 months after iron deficiency anemia diagnosis. Data calculated with Nutrisurvey® and STATA 12® for paired T-test. Result: No significant changes (p>0,05) in energy, protein, fat, zinc, vitamin B12, copper, fiber, tannin, and oxalate intake. There were significant improvement in intake of carbohydrate (p=0,0161), iron (p=0,0057), phytate (p=0,000), and vitamin C (p=0,0017). No significant changes in mean intake of fruit, vegetable, and tea servings (p>0,05), but higher consumption of coffee (p=0,0018). Conclusion: Iron deficiency anemia diagnosis resulted in dietary intake changes. Subjects were more aware of their dietary intake after iron deficiency anemia diagnosis. Small fruit and vegetable intake and high tea and coffee consumption suggested that efforts were needed to encourage dietary changes in these foods. Keywords: dietary changes, eating habit, adolescent girl, iron deficiency anemia
The expression of GLUT-1 and VEGF-A mRNA in the rectal cancer patients with neoadjuvant chemoradiation Sri Nuryani Wahyuningrum; Christina Prihharsanti; Sofia Mubarika Haryana; Ahmad Ghozali; Firly Putri Fardhila
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3149

Abstract

Latar belakang: Angka kekambuhan dan resistensi pasien kanker rektum mencapai 40 persen. Kondisi tersebut bisa disebabkan karena peningkatan ekspresi GLUT-1 dan VEGF-A, serta mempengaruhi prognosis pasien. Tujuan penelitian ini adalah untuk mengetahui korelasi ekspresi mRNA GLUT-1 dan VEGF-A, serta hubungannya dengan prognosis pasien kanker rektum yang menjalani kemoradiasi. Metode: Penelitian Kohor ini melibatkan 16 orang pasien kanker rektum lokal stadium II atau III yang menjalani kemoradiasi di RSUP Kariadi Semarang. Sampel darah intravena diambil 5 mL pada saat sebelum dan sesudah kemoradiasi. Total RNA diisolasi dari 200 μl serum, kemudian dilakukan sintesis cDNA. Ekspresi mRNA GLUT-1 dan VEGF-A dikuantifikasi dengan metode Livak menggunakan reference gene β-actin. Hasil: Rata-rata ekspresi mRNA GLUT-1 menurun signifikan 2,14 kali (P=0,044) dan mRNA VEGF-A menurun 1,9 kali (P = 0,03). Ekspresi mRNA GLUT-1 dan VEGF-A berkorelasi positif kuat dan signifikan pada saat sebelum (r = 0,6; R2 = 0,455; P = 0,013) dan sesudah kemoradiasi (r = 0,8; R2 = 0,598; P < 0,001). Peningkatan ekspresi mRNA GLUT-1 berhubungan dengan prognosis buruk pasien, dengan resiko 18 kali lebih tinggi (P=0,036; OR=18, 95% CI=1,2 – 261). Peningkatan ekspresi mRNA VEGF-A tidak berhubungan signifikan dengan prognosis pasien (P=0,12; OR=9; 95%CI=0,6-123). Kesimpulan: Ekspresi mRNA GLUT-1 dan VEGF-A berkorelasi positif dan saling mempengaruhi satu dengan lainnya. Peningkatan ekspresi mRNA GLUT-1 berhubungan dengan prognosis buruk pasien. Hasil penelitian ini mengindikasikan bahwa ekspresi mRNA GLUT-1 dari sampel darah berpotensi sebagai biomarker prognosis pada pasien kanker rektum yang menjalani kemoradiasi. Kata kunci: kanker rektum, kemoradiasi, GLUT-1, VEGF-A, prognosis Abstract Background: Rectal cancer patients have 40 percent risk of recurrence and resistance, which is triggered by increasing in GLUT-1 and VEGF-A mRNA expression. This condition associate with the patients prognosis. This study aimed to determine the correlation between GLUT-1 and VEGF-A mRNA expression, and analyze its association with the rectal cancer patients prognosis who received chemoradiation. Methods: This was a Cohort study involving 16 rectal cancer patients with stage II or III undergoing chemoradiation at Kariadi Hospital Semarang. Five milliliters of intravenous blood samples were taken before and after chemoradiation. Total RNA was isolated from 200 μl of blood serum, followed by cDNA synthesis. GLUT-1 and VEGF-A mRNA expression was quantified by the Livak method using β-actin as a reference gene. Results: GLUT-1 and VEGF-A mRNA expression decreased significantly 2.14 times (P=0,044) and 1,9 times (P=0,03), respectively. Expression of GLUT-1 and VEGF-A mRNA have a significant and strong positive correlation at before (r=0,6; R2=0,455; P=0,013) and after chemoradiation (r=0,8; R2=0,598; P<0,001). GLUT-1 mRNA expression enhancement significantly associate with poor prognosis and risk 18 times of worse prognosis (P=0,036; OR=18, 95% CI=1,2–261). VEGF-A mRNA expression did not associate with patient prognosis (P=0,12; OR=9; 95%CI=0,6-123). Conclusion: Expression of GLUT-1 and VEGF-A mRNA have a significant and strong positive correlation. GLUT-1 mRNA expression enhancement significantly associate with the poor prognosis of the rectal cancer patients. Our finding suggests that GLUT-1 mRNA expression from blood sample was potential as a biomarker to predict rectal cancer patient prognosis who received chemoradiation Keywords: rectal cancer, chemoradiotherapy, GLUT-1, VEGF-A, prognosis
Risk factors associated with Dengue incidence in Bandung, Indonesia: a household based case-control study hubullah fuadzy; Mutiara Widawati; Endang P. Astuti; Heni Prasetyowati; Joni Hendri; Rohmansyah W. Nurindra; Dewi N. Hodijah
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i1.3150

Abstract

Latar belakang: Bandung memiliki daerah perkotaan dengan kualitas bangunan rumah yang memadai, namun masih memiliki kasus endemik Demam Berdarah Dengue (DBD) yang tinggi. Penelitian ini bertujuan untuk mengetahui karakteristik kejadian demam berdarah di tingkat rumah tangga. Metode: Data dianalisis dari 781 rumah tangga yang terdiri dari 261 kasus dan 522 kontrol. Pemilihan sampel menggunakan metode kasus kontrol berpasangan dengan rasio 1:2. Tahapan penelitian terdiri dari pengamatan status rumah menggunakan form ceklis pemeriksaan dan wawancara menggunakan kuesioner tertutup. Analisis data korelasi pairwise spearmen, kemudian regresi logistik biner digunakan untuk prediksi faktor risiko. Hasil: Faktor risiko usia produktif dan rendahnya tingkat pendidikan kepala keluarga, toilet yang kotor, dan status rumah tidak sehat memiliki pengaruh yang signifikan terhadap peningkatan kasus demam berdarah di Kota Bandung ((p<0.05). Faktor determinan adalah usia produktif kepala keluarga (31 - 60 tahun) dimana memiliki kemungkinan 2,53 (95%CI 1.34-4.78;p<0.05) kali lebih besar untuk memiliki anggota rumah tangga yang menderita DBD di Bandung. Kesimpulan: Usia dan pendidikan kepala rumah tangga, kebersihan toilet dan status rumah sehat memilikiperan penting dalam mempengaruhi kejadian demam berdarah. Dengan demikian, petugas kesehatanperlu melakukan promosi kesehatan mengenai DBD secara intensif kepada kepala rumah tangga. Kata kunci: Demam Berdarah Dengue (DBD), kepala rumah tangga, usia, sanitasi rumah, Kota Bandung Abstract Background: Bandung have urban areas with adequate housebuilding quality, yet still has high Dengue endemic cases. This study aims to investigate the characteristics of dengue incidence at the household level. Methods: Data analyzed from 781 households consisted of 261 cases and 522 controls. We applied matched case-control samples with a ratio of 1:2 (case: controls). The stages of the research consisted of a house status observation using a form inspection checklist and interviews using a closed-ended questionnaire. The data analyzed by pairwise spearmen correlation and binary logistic regression for risk factor prediction. Results: Risk factors for productive age and low level of education of family heads, dirty toilets, and unhealthy house status have a significant effect on the increase of dengue cases in Bandung (p<0.05). The determinant factor is the productive age of the head of the family (31 - 60 years), it means that the family who has a family head in a productive age is 2.53 (95% CI 1.34-4.78; p <0.05) times more likely to have a household member suffering from DHF in Bandung. Conclusion: The age and the level of education of the household heads, toilet hygiene, and healthy home status have an essential role in influencing dengue fever. Therefore, health workers need to perform an intensive health promotion regarding DHF to the household heads. Keywords: Dengue, head of household, ages, house sanitation, Bandung City
Front Matter HSJi Volume 11 no.2 2019 hsji author
Health Science Journal of Indonesia Vol 10 No 2 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Front Matter HSJi Volume 11 no.2 2019
Front Matter HSJi Volume 11 no.1 2020 hsji author
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Front Matter HSJi Volume 11 no.2 2019
In silico analysis of antihypertensive and hepatotoxicity potential of the n-butanol fraction of the methanol extract of of cantaloupe (Cucumis melo var. cantalupensis) Dian Laila Purwaningroom; Dianita Rifqia Putri; Galuh Wening Permatasari
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3629

Abstract

Latar belakang: Hipertensi merupakan faktor risiko utama penyakit kardiovaskular. Penduduk Indonesia cenderung mengkonsumsi herbal dalam terapi hipertensi dalam mempertahankan kadar tekanan darah seperti buah blewah (Cucumis melo var. cantalupensis). Namun mekanisme kerja buah blewah dalam menurunkan tekanan darah, dan potensi toksisitasnya jika dikonsumsi dalam jangka panjang masih belum jelas. Tujuan studi ini adalah untuk menganalisis mekanisme antihipertensi dari buah blewah dan potensi toksiknya melalui pendekatan in silico. Metode: Bubuk blewah kering dimaserasi menggunakan metanol absolut, difraksinasi menggunakan n-butanol. Uji fitokimia dilakukan dengan metode LC-MS, kemudian senyawa bioaktif ditelusuri hingga SMILESnya di PubChem. Analisis QSAR untuk analisis potensi antihipertensi dilakukan dengan PASS server. Kelas toksisitas dan potensi hepatotoksisitas dianalisis menggunakan ProTox-II, dilanjutkan dengan analisis networking menggunakan STITCH dan STRINGdb. Hasil: Setidaknya terdapat 434 jenis senyawa yang terdapat pada fraksi n-butanol dari ekstrak metanol buah blewah (FBEMB). Berdasarkan analisis STITCH dan STRINGdb, FBEMB dapat bekerja dalam menurunkan tekanan darah melalui mekanisme aksi seperti senyawa amlodipine, yang menstabilkan saluran kalsium tipe-L yang terisi tegangan dalam konformasi tidak aktifnya. Dengan demikian, mencegah kontraksi myocyte yang bergantung pada kalsium dan vasokonstriksi. FBEMB mungkin berpotensi hepatotoksik melalui mekanisme kerja senyawa seperti itrakonazol yang menghambat enzim sitokrom P450 yang mempengaruhi gangguan pada sintesis ergosterol, dan efavirenz yang memiliki efek neurotoksik. Penghambatan sitokrom P450 dapat menyebabkan toksisitas obat dan kerusakan hati. Kesimpulan: FBEMB dapat bekerja dalam menurunkan tekanan darah melalui mekanisme penstabilan saluran kalsium tipe-L yang terisi tegangan dalam konformasi tidak aktifnya. Kata kunci: in silico, antihipertensi, hepatotoksisitas, blewah Abstract Background: Hypertension is a major cardiovascular disease risk factor. Indonesian people tend to consume herbal medicine to maintain hypertension therapy, i.e cantaloupe (Cucumis melo var. cantalupensis). However, the mechanism of action of cantaloupe in lowering blood pressure and toxicity potential for long term consumption is unclear. The study aimed to analyze the antihypertensive mechanism of cantaloupe and its toxic potential through the in silico. Methods: The dried cantaloupe powder was macerated using absolute methanol, then fractionated using n-butanol. The phytochemical test was done by LC-MS method, then the bioactive compounds were traced to their SMILES in the PubChem. The QSAR analysis of the antihypertensive potential was done using the PASS server. The toxicity class and hepatotoxicity potential were analyzed using ProTox-II, followed by networking analysis using STITCH and STRINGdb. Results: At least 434 types of compounds were shown in the n-butanol fraction of the methanol extract of cantaloupe (BFMEC). Based on the networking analysis, BFMEC may work in lowering blood pressure through the action mechanism of the amlodipine compound-like, which stabilizes voltage-gated L-type calcium channels in an inactive conformation, thus, prevents calcium-dependent myocyte contraction and vasoconstriction. BFMEC presumably has hepatotoxic through the action mechanism of itraconazole compound-like inhibited cytochrome P450-dependent enzymes, affecting the impairment of ergosterol synthesis, and efavirenz which has neurotoxic effects. The inhibition of cytochrome P450 may cause drug toxicity and liver damage. Conclusion: BFMEC may work in lowering blood pressure through the action mechanism which stabilizes voltage-gated L-type calcium channels in an inactive conformation. Keywords: in silico, antihypertensive, hepatotoxicity, cantaloupe
In silico analysis of V48A dihydropteroate synthase mutation to dapsone on Mycobacterium leprae from Papua Yustinus Maladan; Hana Krismawati; Hotma Martogi Laurensia Hutapea; Antonius Oktavian
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.3744

Abstract

Latar belakang: Lepra merupakan penyakit yang disebabkan oleh Mycobacterium leprae. Resistensi obat merupakan salah satu tantangan dalam pemberantasan kusta khususnya di Papua. Adanya mutasi pada gen folP1 penyandi dihydropteroate synthase (DHPS) merupakan dasar untuk deteksi molekuler resistensi dapson pada penyakit lepra. Tujuan penelitian ini adalah mendeteksi mutasi pada gen folP1 Mycobacterium leprae dari Papua, Indonesia dan menganalisis pengaruh mutasi tersebut terhadap dapson dengan metode in silico. Metode: Identifikasi mutasi pada gen folp1 M. leprae dilakukan melalui proses Basic Local Alignment Search Tool (BLAST) di gene bank. Analisis efek mutasi dengan menggunakan server Have (y) Our Protein Explained (HOPE). Prediksi binding pocket menggunakan Computed Atlas of Surface Topography of proteins (CASTp). Homologi modeling struktur 3D DHPS menggunakan server Iterative Threading ASSEmbly Refinement (I TASSER). Analisis docking dengan menggunakan AutoDock Vina yang terintegrasi dengan aplikasi Python Prescription (PyRx). Hasil: Hasil sekuensing menunjukkan adanya variasi dalam gen folP1 M. leprae yaitu perubahan dari Timin (T) menjadi Sitosin (C) pada nukleotida 143. Residu yang bermutasi (V48A) terletak pada domain yang penting untuk aktivitas protein dan kontak dengan residu di domain lain. Ada kemungkinan bahwa interaksi ini penting untuk fungsi protein secara benar. Mutan V48A tidak banyak mempengaruhi stabilitas dari dihydropteroate synthase M. leprae. Kesimpulan: Berdasarkan analisis molecular docking, mutasi V48A tidak mempengaruhi binding affinity dapson terhadap dihydropteroate synthase M. leprae. Hasil ini menunjukkan mutan V48A kemungkinan tetaprentan terhadap dapson. Dengan demikian perlu dilakukan uji in vivo untuk mengkofirmasi efek mutasi V48A. Kata kunci: Mycobacterium leprae, folP1 gene, dihydropteroate synthase, dapson Abstract Background: Leprosy is a disease caused by Mycobacterium leprae. Drug resistance is one of the challenges in leprosy elimination especially in Papua. The presence of mutations in folP1 gene that encode dihydropteroate synthase (DHPS) was considered as the exclusive basis for molecular detection of dapsone resistance in leprosy. The objective of this study was to detect mutations in the folP1 gene of Mycobacterium leprae from Papua, Indonesia and to analyze the effect of these mutations on dapsone using the in-silico method. Methods: Identification of mutations in the folp1 M. leprae gene is carried out through the Basic Local Alignment Search Tool (BLAST) process in the gene bank. The analysis of the effects of mutations using the Have (y)Our Protein Explained (HOPE) server. Bindings pocket prediction is done using the Computed Atlas of Surface Topography of proteins (CASTp). Homology modeling 3D structure of DHPS using the Iterative Threading ASSEmbly Refinement (I-TASSER) server. Docking analysis was performed using AutoDock Vina which is integrated with the Python Prescription (PyRx) application. Results: The sequencing results showed a variation in the folP1 M. leprae gene, namely a change from thymine (T) to cytosine (C) in nucleotide 143. The mutated residue (V48A) is in a domain that is essential for the activity of the protein and in contact with residues in another domain. It is possible that this interaction is important for the correct function of the protein. V48A mutants did not significantly affect the stability of DHPS M. leprae. Conclusion: Based on molecular docking analysis, this mutation does not affect binding affinity dapsone against M. leprae dihydropteroate synthase. These results indicate that the V48A mutant is likely to remain susceptible to dapsone. Thus, it is necessary to do an in vivo test to confirm the effect of the V48A mutation. Keywords: Mycobacterium leprae, folP1 gene, dihydropteroate synthase, dapsone

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